RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Lingcod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ophiodon elongatus
FDA Seafood List Common Name: Lingcod
FDA Seafood List Market Name: Lingcod
Unique IEF Gel Code: 19950831gel03
Unique Web Code: lch01Srr
Tissue Aliquot Code: ophielon I94-001 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	78	9.6	0.0550599	5	0.443917	12.8	8.8	4.55	78.2	4.7	0.053687
2	5.2	181	22.3	0.0304734	6	0.300222	8.7	5.9	5.2	180.3	5.7	0.0295454
3	5.85	276	34.1	0.0404616	4	0.278335	8	5.5	5.85	276.3	3.9	0.040361
4	6.55	394	48.6	0.0447723	12	0.820915	23.7	16.2	6.55	390.9	12.2	0.0380781
5	7.35	613	75.7	0.0420059	8	0.517604	14.9	10.2	7.35	612.6	7.7	0.0377101
6	8.15	669	82.6	0.0415256	7	0.513767	14.8	10.2	8.15	667.9	7.2	0.040211
7	8.45	725	89.5	0.0249758	6	0.256319	7.4	5.1	8.45	723.3	5.8	0.0249521
8	8.65	793	97.9	0.0233903	9	0.331598	9.6	6.6	8.65	792.9	8.7	0.0214633

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.167	19	2.3	0.132144	7	1.71123	6.1	4.6	4.167	19.7	7.4	0.130533
2	4.414	57	7	0.0750516	8	0.967379	3.5	2.6	4.414	56.1	8	0.0680497
3	4.453	63	7.8	0.0558314	5	0.348107	1.2	0.9	4.453	65.4	5.3	0.0368219
4	4.511	72	8.9	0.0477252	3	0.221321	0.8	0.6	4.511	72.7	3.2	0.0389985
5	4.563	80	9.9	0.0115428	2	0.0506546	0.2	0.1	4.563	81.9	2	0.0140001
6	4.648	93	11.5	0.0115784	2	0.0454272	0.2	0.1	4.648	93.3	2.2	0.0117275
7	4.732	106	13.1	0.0142891	4	0.1197	0.4	0.3	4.732	107.1	4.4	0.0155212
8	4.998	147	18.1	0.182694	6	1.71566	6.1	4.6	4.998	146.8	5.5	0.175137
9	5.494	225	27.7	0.0323041	3	0.17695	0.6	0.5	5.494	224.2	3.2	0.0315634
10	5.588	240	29.6	0.056311	7	0.595538	2.1	1.6	5.588	237.7	6.9	0.0490378
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.737	264	32.6	0.528364	4	3.50045	12.5	9.4	5.737	264.7	3.8	0.513928
12	5.856	283	34.9	0.171253	9	2.39271	8.6	6.4	5.856	282.5	9.5	0.142163
13	6.003	307	37.9	0.382767	3	1.90917	6.8	5.1	6.003	307.3	2.8	0.385927
14	6.168	334	41.2	0.160889	5	1.37557	4.9	3.7	6.168	332.9	5.1	0.153669
15	6.229	344	42.4	0.0271566	2	0.0549654	0.2	0.1	6.229	345.2	1.6	0.019234
16	6.278	352	43.4	0.135187	2	0.437116	1.6	1.2	6.278	352	2.2	0.114396
17	6.321	359	44.3	0.259533	4	1.84045	6.6	4.9	6.321	358.7	4.4	0.234832
18	6.48	385	47.5	0.0488929	7	0.661739	2.4	1.8	6.48	386.6	7.3	0.0509735
19	6.567	401	49.4	0.0947923	6	0.980472	3.5	2.6	6.567	401.3	5.9	0.0944857
20	6.626	417	51.4	0.260569	2	1.0498	3.8	2.8	6.626	417.1	2.3	0.258403
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.714	441	54.4	0.010958	3	0.0684064	0.2	0.2	6.714	439.9	3.1	0.0125648
22	6.851	478	58.9	0.0743125	4	0.502411	1.8	1.3	6.851	478.2	4.2	0.0682054
23	6.984	514	63.4	0.0471139	14	1.04697	3.7	2.8	6.984	514.7	13.5	0.0436553
24	8.447	725	89.4	0.236965	15	6.20457	22.2	16.6	8.447	725.8	14.9	0.234827

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	78	9.6	0.0318274	8	0.484957	16.5	11.4	4.55	79.1	8	0.0340912
2	5.2	175	21.6	0.0133486	6	0.132926	4.5	3.1	5.2	175.4	6.2	0.0121891
3	5.85	288	35.5	0.0194848	9	0.308861	10.5	7.3	5.85	284.4	9.3	0.0187933
4	6.55	399	49.2	0.037431	9	0.533929	18.2	12.6	6.55	397.2	8.8	0.0343021
5	7.35	613	75.6	0.0301664	8	0.368504	12.6	8.7	7.35	613.2	7.6	0.0275314
6	8.15	672	82.9	0.0331237	8	0.437242	14.9	10.3	8.15	671.1	7.9	0.0312004
7	8.45	726	89.5	0.0313637	5	0.243959	8.3	5.7	8.45	725.2	4.5	0.0304646
8	8.65	785	96.8	0.0229614	13	0.423561	14.4	10	8.65	783.6	13.1	0.0183057

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.225	32	3.9	0.067911	10	1.19829	6.6	5.2	4.225	33.4	10.3	0.0653881
2	4.457	67	8.3	0.0349152	6	0.342172	1.9	1.5	4.457	65.1	5.6	0.0347087
3	4.55	81	10	0.0794718	9	1.09772	6.1	4.8	4.55	81.7	8.6	0.0721596
4	5.041	155	19.1	0.0928017	7	1.11351	6.1	4.8	5.041	154	7.2	0.0871597
5	5.2	179	22	0.017287	4	0.126188	0.7	0.5	5.2	178.7	4.2	0.0171515
6	5.5	227	28	0.0115456	6	0.108714	0.6	0.5	5.5	227.7	5.8	0.0105664
7	5.637	249	30.7	0.0243443	4	0.147591	0.8	0.6	5.637	248.6	3.7	0.0225431
8	5.787	273	33.6	0.370133	4	2.621	14.4	11.4	5.787	272.7	4.1	0.364192
9	5.874	287	35.3	0.139996	6	1.30038	7.2	5.6	5.874	287.3	5.8	0.125449
10	6.021	311	38.3	0.272665	3	1.40718	7.8	6.1	6.021	311.2	2.9	0.272386
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.187	338	41.6	0.13821	5	1.1772	6.5	5.1	6.187	337.1	4.9	0.134654
12	6.254	349	43	0.00922602	1	0.0192049	0.1	0.1	6.254	348.6	1	0.011046
13	6.297	356	43.8	0.073625	2	0.299739	1.7	1.3	6.297	356.3	2.2	0.0755059
14	6.34	363	44.7	0.180296	3	0.887849	4.9	3.9	6.34	362.9	3.2	0.154937
15	6.517	392	48.3	0.0423626	8	0.602869	3.3	2.6	6.517	392	8	0.0427063
16	6.579	405	49.9	0.0582503	5	0.522365	2.9	2.3	6.579	405.3	5.2	0.0563869
17	6.635	420	51.7	0.121206	2	0.542912	3	2.4	6.635	420.5	2.5	0.124684
18	6.72	443	54.6	0.00658537	3	0.0354734	0.2	0.2	6.72	443.3	3.3	0.0060211
19	6.866	482	59.4	0.0149065	3	0.107463	0.6	0.5	6.866	480.2	2.6	0.0236235
20	6.982	513	63.2	0.0247772	9	0.371546	2	1.6	6.982	513.5	8.8	0.023954
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.15	674	83	0.0138102	6	0.156307	0.9	0.7	8.15	672.4	6.5	0.0135705
22	8.45	727	89.5	0.164061	13	3.61985	20	15.7	8.45	726.7	12.9	0.158035
23	8.65	785	96.7	0.0187264	12	0.333991	1.8	1.5	8.65	784.3	12.4	0.0151459

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.227	27	3.3	0.0971275	11	1.91906	6.7	5.1	4.227	28.6	11.3	0.0957367
2	4.466	64	7.9	0.168681	13	3.94818	13.9	10.5	4.466	66.3	13.3	0.167657
3	4.531	74	9.1	0.138734	8	0.6834	2.4	1.8	4.531	83.4	7.8	0.0491199
4	5.016	149	18.3	0.129805	6	1.37554	4.8	3.7	5.016	148.3	6.3	0.122297
5	5.501	224	27.6	0.023085	3	0.122263	0.4	0.3	5.501	223.9	3	0.0231746
6	5.604	240	29.6	0.040045	3	0.227288	0.8	0.6	5.604	239.8	3.4	0.0381754
7	5.747	262	32.3	0.415266	4	2.90615	10.2	7.7	5.747	262.4	4	0.412194
8	5.837	276	34	0.135642	9	2.0864	7.3	5.6	5.837	276.2	8.9	0.131865
9	5.987	301	37.1	0.184554	5	1.58453	5.6	4.2	5.987	302.1	4.8	0.187493
10	6.165	331	40.8	0.125797	6	1.34826	4.7	3.6	6.165	330.1	6.2	0.122613
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.243	344	42.4	0.0265467	3	0.0876208	0.3	0.2	6.243	344.4	2.9	0.0170007
12	6.284	351	43.2	0.118186	3	0.54278	1.9	1.4	6.284	350.7	2.9	0.107272
13	6.32	357	44	0.220867	4	1.31209	4.6	3.5	6.32	357.2	3.5	0.209334
14	6.368	365	45	0.0522519	5	0.404137	1.4	1.1	6.368	364.9	4.6	0.0491224
15	6.504	388	47.8	0.0488115	8	0.679926	2.4	1.8	6.504	388.4	7.7	0.0497918
16	6.574	402	49.5	0.0924642	6	0.898427	3.2	2.4	6.574	402.2	5.6	0.0899497
17	6.63	417	51.4	0.204921	2	0.853931	3	2.3	6.63	417.5	2.3	0.209587
18	6.719	441	54.3	0.00867675	4	0.0636074	0.2	0.2	6.719	441	4	0.00898371
19	6.861	479	59	0.0623146	4	0.393401	1.4	1	6.861	478.8	3.8	0.0583843
20	6.984	512	63.1	0.0532293	10	0.906721	3.2	2.4	6.984	511	10.2	0.049987
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.439	724	89.2	0.275129	13	6.12151	21.5	16.3	8.439	723	13.1	0.264331

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	73	9	0.0481035	5	0.429928	14.7	10.2	4.55	74	5.2	0.0466088
2	5.2	176	21.6	0.0229052	6	0.250374	8.5	6	5.2	175.6	6.3	0.0222558
3	5.85	273	33.6	0.0304639	5	0.275252	9.4	6.5	5.85	273.5	5.1	0.0303498
4	6.55	394	48.5	0.0210755	10	0.38145	13	9.1	6.55	391.7	9.9	0.0216563
5	7.35	609	74.9	0.0348867	8	0.445692	15.2	10.6	7.35	608.1	7.6	0.0329865
6	8.15	669	82.3	0.0310657	9	0.438532	15	10.4	8.15	667.3	8.6	0.02869
7	8.45	725	89.2	0.0346503	5	0.271665	9.3	6.5	8.45	724.3	4.7	0.0322749
8	8.65	788	96.9	0.0302907	10	0.436561	14.9	10.4	8.65	787.3	9.5	0.0258594

PeakCounter: 8

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 92

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 12.800 5.200 8.700 5.850 8.000 6.550 23.700 7.350 14.900 8.150 14.800 8.450 7.400 8.650 9.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 8 Return to Lane Navigator
4.167 6.100 4.414 3.500 4.453 1.200 4.511 0.800 4.563 0.200 4.648 0.200 4.732 0.400 4.998 6.100 5.494 0.600 5.588 2.100 5.737 12.500 5.856 8.600 6.003 6.800 6.168 4.900 6.229 0.200 6.278 1.600 6.321 6.600 6.480 2.400 6.567 3.500 6.626 3.800 6.714 0.200 6.851 1.800 6.984 3.700 8.447 22.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 24 Return to Lane Navigator
4.550 16.500 5.200 4.500 5.850 10.500 6.550 18.200 7.350 12.600 8.150 14.900 8.450 8.300 8.650 14.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 8 Return to Lane Navigator
4.225 6.600 4.457 1.900 4.550 6.100 5.041 6.100 5.200 0.700 5.500 0.600 5.637 0.800 5.787 14.400 5.874 7.200 6.021 7.800 6.187 6.500 6.254 0.100 6.297 1.700 6.340 4.900 6.517 3.300 6.579 2.900 6.635 3.000 6.720 0.200 6.866 0.600 6.982 2.000 8.150 0.900 8.450 20.000 8.650 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 23 Return to Lane Navigator
4.227 6.700 4.466 13.900 4.531 2.400 5.016 4.800 5.501 0.400 5.604 0.800 5.747 10.200 5.837 7.300 5.987 5.600 6.165 4.700 6.243 0.300 6.284 1.900 6.320 4.600 6.368 1.400 6.504 2.400 6.574 3.200 6.630 3.000 6.719 0.200 6.861 1.400 6.984 3.200 8.439 21.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 21 Return to Lane Navigator
4.550 14.700 5.200 8.500 5.850 9.400 6.550 13.000 7.350 15.200 8.150 15.000 8.450 9.300 8.650 14.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 8 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Lingcod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Lingcod ---- Go to Display Gel Lane Navigator.