Gartner S, Liu Y, Lewis M, Polonis V, Hunter E, Brown C, Elkins WR, Zack P, Eddy G; Symposium on Nonhuman Primate Models for AIDS.
J Med Primatol. 1993 Sep-Oct; 22: abstract no. 37.
Henry M. Jackson Foundation, Rockville, MD.
Prior to animal studies, in vitro experiments were performed to assess the relative susceptibility of cells from various monkey species to HIV-1. We found: (1) Cultured T cells from pig-tailed macaques were more susceptible and/or permissive for HIV-1 than T cells from cynomolgus and rhesus monkeys, although peak expression for the pig-tailed macaque cells was only 1/10 to 1/100th that for human cells. (2) The majority of HIV-1 isolates were unable to productively infect pig-tailed macaque cells, regardless of the host cell of origin of the viral inoculum. (3) The level of HIV-1 expression in healthy, ongoing pig-tailed macaque T cell cultures could not be boosted by the addition of fresh autologous or allogeneic PHA-activated pig-tailed macaque T cells, and cell-free virus derived from macaque cells was poorly infectious. Semi-quantitative PCR analyses and virus rescue experiments suggested that suboptimal HIV-1 infection of macaque cells may be a consequence of limited permissivity rather than susceptibility. We then inoculated 4 pig-tailed macaques with autologous, HIV-1-expressing cells. During the first 10 weeks, infectious virus was recovered from blood leukocytes and lymph nodes, but no HIV-1-specific antibodies were detectable. Subsequently, HIV-1 could not be isolated from blood leukocytes, although the cells were frequently PCR positive. Between weeks 10-15, animals began to develop antibodies to HIV-1 p17, p24, gp120 and gp160 proteins as determined by Western blot, and levels of these antibodies have increased with time. Recently, at week 36, infectious virus was recovered from blood leukocytes from one of the animals. Active virus replication appears to be ongoing in these animals, pointing to the possibility for improved adaptation of HIV-1 to this species.
Publication Types:
Keywords:
- Animals
- Gene Products, env
- HIV Antibodies
- HIV Envelope Protein gp120
- HIV Infections
- HIV-1
- Humans
- In Vitro
- Macaca
- Macaca mulatta
- Sus scrofa
- Swine
- Virus Replication
- immunology
- virology
Other ID:
UI: 102207765
From Meeting Abstracts