MT0128

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

46 ESTs from this library were grouped into 31 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
7	152174		Hs.528305	DEAD (Asp-Glu-Ala-Asp) box polypeptide 17.
3	65217		Hs.3260	Presenilin 1.
2	43478		Hs.716898	Transcribed locus.
2	43478		Hs.675962	CDNA FLJ33840 fis, clone CTONG2004912.
2	43478		Hs.528324	Transcribed locus.
2	43478		Hs.477440	Oxysterol binding protein-like 11.
2	43478		Hs.381099	Lymphocyte cytosolic protein 1 (L-plastin).
2	43478		Hs.282410	Calmodulin 1 (phosphorylase kinase, delta).
2	43478		Hs.144130	Solute carrier family 25, member 36.
1	21739		Hs.89649	Epoxide hydrolase 1, microsomal (xenobiotic).
1	21739		Hs.708270	Transcribed locus.
1	21739		Hs.679116	Transcribed locus.
1	21739		Hs.658464	GINS complex subunit 1 (Psf1 homolog).
1	21739		Hs.657569	Chondroitin sulfate N-acetylgalactosaminyltransferase 2.
1	21739		Hs.654481	Tumor protein p53.
1	21739		Hs.645309	Embigin homolog (mouse).
1	21739		Hs.608498	MRNA; cDNA DKFZp564H092 (from clone DKFZp564H092).
1	21739		Hs.570362	Virus-induced signaling adapter.
1	21739		Hs.529517	Lactotransferrin.
1	21739		Hs.524530	CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2.
1	21739		Hs.507122	SET domain containing 1B.
1	21739		Hs.473937	NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa.
1	21739		Hs.458272	Myeloperoxidase.
1	21739		Hs.408241	Nucleoporin like 2.
1	21739		Hs.369438	V-ets erythroblastosis virus E26 oncogene homolog 1 (avian).
1	21739		Hs.369265	Interleukin-1 receptor-associated kinase 3.
1	21739		Hs.290404	Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3.
1	21739		Hs.213690	WD repeat domain 70.
1	21739		Hs.181855	Cancer susceptibility candidate 5.
1	21739		Hs.181301	Cathepsin S.
1	21739		Hs.180877	H3 histone, family 3B (H3.3B).

Source: NCBI UniGene

EST Sequences

151 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• blood
• leukemia
• adult

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).