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Library:7619 (dbEST ID)
CI0154
Library Description
| Organism: | Homo sapiens |
| Developmental stage: | Adult |
| Organ: | colon_ins |
| Vector: | puc18 |
| Vector type: | Plasmid |
| Cloning site 1: | SmaI |
| Cloning site 2: | SmaI |
A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.
Source: Simpson A.J.G., Ludwig Institute for Cancer Research
Gene Content Analysis
122 ESTs from this library were grouped into 97 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 8 | 65574 |  |
Hs.625725 | Basal cell adhesion molecule (Lutheran blood group). |
| 5 | 40984 | |
Hs.433343 | Serine/arginine repetitive matrix 2. |
| 3 | 24590 | |
Hs.653312 | Transcribed locus. |
| 3 | 24590 | |
Hs.300816 | RAB1B, member RAS oncogene family. |
| 2 | 16393 | |
Hs.700112 | Immunoglobulin heavy locus. |
| 2 | 16393 | |
Hs.590958 | FBJ murine osteosarcoma viral oncogene homolog B. |
| 2 | 16393 | |
Hs.548018 | LIM and SH3 protein 1. |
| 2 | 16393 | |
Hs.511605 | Annexin A2. |
| 2 | 16393 | |
Hs.502842 | Calpain 1, (mu/I) large subunit. |
| 2 | 16393 | |
Hs.446852 | Eukaryotic translation initiation factor 3, subunit L. |
| 2 | 16393 | |
Hs.290758 | Damage-specific DNA binding protein 1, 127kDa. |
| 2 | 16393 | |
Hs.270017 | Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa. |
| 2 | 16393 | |
Hs.203717 | Fibronectin 1. |
| 2 | 16393 | |
Hs.154578 | Myosin VIIB. |
| 1 | 8197 |  |
Hs.93002 | Ubiquitin-conjugating enzyme E2C. |
| 1 | 8197 | |
Hs.89649 | Epoxide hydrolase 1, microsomal (xenobiotic). |
| 1 | 8197 | |
Hs.78582 | Developmentally regulated GTP binding protein 2. |
| 1 | 8197 | |
Hs.75199 | Protein phosphatase 2, regulatory subunit B', beta isoform. |
| 1 | 8197 | |
Hs.75069 | Serine hydroxymethyltransferase 2 (mitochondrial). |
| 1 | 8197 | |
Hs.74576 | GDP dissociation inhibitor 1. |
| 1 | 8197 | |
Hs.73090 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100). |
| 1 | 8197 | |
Hs.716105 | Transcribed locus, moderately similar to NP_001099421.1 phosphoinositide-3-kinase, class 2, beta polypeptide [Rattus norvegicus]. |
| 1 | 8197 | |
Hs.708975 | EF-hand calcium binding domain 4A. |
| 1 | 8197 | |
Hs.703889 | Immunoglobulin heavy chain variable region (IGHV gene), clone slvl18. |
| 1 | 8197 | |
Hs.694019 | Transcribed locus, moderately similar to NP_000880.1 integrin, beta 7 [Homo sapiens]. |
| 1 | 8197 | |
Hs.679335 | Transcribed locus. |
| 1 | 8197 | |
Hs.673372 | Transcribed locus, strongly similar to NP_001129575.1 transmembrane protein 132C [Homo sapiens]. |
| 1 | 8197 | |
Hs.661431 | Hypothetical protein LOC100130275. |
| 1 | 8197 | |
Hs.655734 | Similar to GLI-Kruppel family member HKR1. |
| 1 | 8197 | |
Hs.655431 | Leukotriene B4 receptor. |
| 1 | 8197 | |
Hs.654389 | Cut-like homeobox 1. |
| 1 | 8197 | |
Hs.647718 | V-set and immunoglobulin domain containing 8. |
| 1 | 8197 | |
Hs.641214 | Transcribed locus. |
| 1 | 8197 | |
Hs.591104 | Bcl2 modifying factor. |
| 1 | 8197 | |
Hs.562015 | Transcribed locus. |
| 1 | 8197 | |
Hs.555947 | Lymphoid enhancer-binding factor 1. |
| 1 | 8197 | |
Hs.533782 | Keratin 8. |
| 1 | 8197 | |
Hs.533282 | Non-POU domain containing, octamer-binding. |
| 1 | 8197 | |
Hs.533273 | Ubiquitin-like modifier activating enzyme 1. |
| 1 | 8197 | |
Hs.5302 | Lectin, galactoside-binding, soluble, 4. |
| 1 | 8197 | |
Hs.529755 | SHANK-associated RH domain interactor. |
| 1 | 8197 | |
Hs.529555 | Transcribed locus, strongly similar to NP_032998.1 prothymosin alpha [Mus musculus]. |
| 1 | 8197 | |
Hs.528007 | U2 small nuclear RNA auxiliary factor 2. |
| 1 | 8197 | |
Hs.524763 | Two pore segment channel 1. |
| 1 | 8197 | |
Hs.524214 | Myeloid leukemia factor 2. |
| 1 | 8197 | |
Hs.515053 | Amino-terminal enhancer of split. |
| 1 | 8197 | |
Hs.514051 | G protein-coupled receptor kinase interacting ArfGAP 1. |
| 1 | 8197 | |
Hs.513242 | Ras homolog gene family, member T2. |
| 1 | 8197 | |
Hs.510635 | Immunoglobulin heavy constant gamma 1 (G1m marker). |
| 1 | 8197 | |
Hs.504998 | Uridine-cytidine kinase 1-like 1. |
| 1 | 8197 | |
Hs.502823 | Peroxiredoxin 5. |
| 1 | 8197 | |
Hs.502116 | Neuron navigator 2. |
| 1 | 8197 | |
Hs.500822 | F-box and WD repeat domain containing 4. |
| 1 | 8197 | |
Hs.485449 | GTP binding protein 2. |
| 1 | 8197 | |
Hs.474213 | Ubiquitin fusion degradation 1 like (yeast). |
| 1 | 8197 | |
Hs.469798 | TSC22 domain family, member 4. |
| 1 | 8197 | |
Hs.464210 | Synaptogyrin 2. |
| 1 | 8197 | |
Hs.462998 | Insulin-like growth factor binding protein 4. |
| 1 | 8197 | |
Hs.461647 | KIAA0182. |
| 1 | 8197 | |
Hs.455336 | Nasal embryonic LHRH factor. |
| 1 | 8197 | |
Hs.442623 | CDNA FLJ30141 fis, clone BRACE2000148. |
| 1 | 8197 | |
Hs.436568 | CD74 molecule, major histocompatibility complex, class II invariant chain. |
| 1 | 8197 | |
Hs.433845 | Keratin 5. |
| 1 | 8197 | |
Hs.432760 | Capping protein (actin filament) muscle Z-line, beta. |
| 1 | 8197 | |
Hs.42853 | Tenascin XB. |
| 1 | 8197 | |
Hs.421907 | Glioma tumor suppressor candidate region gene 2. |
| 1 | 8197 | |
Hs.413748 | Protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3. |
| 1 | 8197 | |
Hs.412842 | Cell division cycle 123 homolog (S. cerevisiae). |
| 1 | 8197 | |
Hs.410977 | Transgelin. |
| 1 | 8197 | |
Hs.405987 | Nucleolar complex associated 2 homolog (S. cerevisiae). |
| 1 | 8197 | |
Hs.386390 | Transcriptional adaptor 3 (NGG1 homolog, yeast)-like. |
| 1 | 8197 | |
Hs.3745 | Milk fat globule-EGF factor 8 protein. |
| 1 | 8197 | |
Hs.370254 | BCL2-associated agonist of cell death. |
| 1 | 8197 | |
Hs.343244 | Adaptor-related protein complex 1, gamma 2 subunit. |
| 1 | 8197 | |
Hs.334684 | Vacuolar protein sorting 26 homolog B (S. pombe). |
| 1 | 8197 | |
Hs.299315 | Dihydropyrimidinase-like 5. |
| 1 | 8197 | |
Hs.292356 | Cytochrome b-245, beta polypeptide. |
| 1 | 8197 | |
Hs.292078 | La ribonucleoprotein domain family, member 1. |
| 1 | 8197 | |
Hs.288959 | Acyl-CoA synthetase family member 2. |
| 1 | 8197 | |
Hs.2785 | Keratin 17. |
| 1 | 8197 | |
Hs.270291 | Actinin, alpha 4. |
| 1 | 8197 | |
Hs.256022 | RAR-related orphan receptor C. |
| 1 | 8197 | |
Hs.246413 | RNA binding motif protein 12. |
| 1 | 8197 | |
Hs.2399 | Matrix metallopeptidase 14 (membrane-inserted). |
| 1 | 8197 | |
Hs.23361 | Ankyrin repeat and BTB (POZ) domain containing 2. |
| 1 | 8197 | |
Hs.22907 | Xylosyltransferase I. |
| 1 | 8197 | |
Hs.211594 | Proteasome (prosome, macropain) 26S subunit, ATPase, 4. |
| 1 | 8197 | |
Hs.204238 | Lipocalin 2. |
| 1 | 8197 | |
Hs.181163 | High-mobility group nucleosomal binding domain 2. |
| 1 | 8197 | |
Hs.179986 | Flotillin 1. |
| 1 | 8197 | |
Hs.172613 | Solute carrier family 12 (potassium/chloride transporters), member 7. |
| 1 | 8197 | |
Hs.160958 | Cell division cycle 37 homolog (S. cerevisiae). |
| 1 | 8197 | |
Hs.152717 | Family with sequence similarity 65, member A. |
| 1 | 8197 | |
Hs.150580 | Eukaryotic translation initiation factor 1. |
| 1 | 8197 | |
Hs.149261 | Runt-related transcription factor 1. |
| 1 | 8197 | |
Hs.1437 | Glucosidase, alpha; acid. |
| 1 | 8197 | |
Hs.119598 | Ribosomal protein L3. |
Source: NCBI UniGene
EST Sequences
206 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- intestine
- colorectal tumor
- adult
Source: NCBI Clone Registry
References
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).