BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract

RepeatMasker Server unavailable.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= B05H02.seq(1>593) (553 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 3 Sequences     : less than 3 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 903 176 |==========================================================
   6310 727 122 |========================================
   3980 605 178 |===========================================================
   2510 427 132 |============================================
   1580 295 108 |====================================
   1000 187  46 |===============
    631 141  43 |==============
    398  98  30 |==========
    251  68  20 |======
    158  48  13 |====
    100  35  12 |====
   63.1  23   7 |==
   39.8  16   7 |==
   25.1   9   2 |:
   15.8   7   1 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 6  <<<<<<<<<<<<<<<<<
   10.0   6   1 |:
   6.31   5   0 |
   3.98   5   0 |
   2.51   5   0 |
   1.58   5   0 |
   1.00   5   0 |
   0.63   5   1 |:
   0.40   4   0 |
   0.25   4   0 |
   0.16   4   0 |
   0.10   4   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|9294516|dbj|BAB02778.1|(AB023036) contains similar... +1   356  1.4e-31   1
gi|9294513|dbj|BAB02775.1|(AB023036) contains similar... +1   335  2.4e-29   1
gi|6319267ref|NP_009350.1| Yal049cp [Saccharomyces ce... +1   114  0.00048   1
gi|8928122|sp|Q9ZT66|E134_MAIZEENDO-1,3;1,4-BETA-D-GL... +1    98  0.076     1
gi|11498723ref|NP_069952.1| hypothetical protein [Arc... +1    71  0.44      1
gi|11359146|pir||T50286hypothetical protein SPAC977.1... +1    80  0.9995    1

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|9294516|dbj|BAB02778.1|  (AB023036) contains similarity to
            endo-1,3-1,4-beta-D-glucanase~gene_id:MDB19.8 [Arabidopsis
            thaliana]
            Length = 239

Frame  1 hits (HSPs):                               ______________________
                        __________________________________________________
Database sequence:     |          |         |          |         |        | 239
                       0         50       100        150       200

  Plus Strand HSPs:

 Score = 356 (125.3 bits), Expect = 1.4e-31, P = 1.4e-31
 Identities = 66/103 (64%), Positives = 83/103 (80%), Frame = +1

Query:     4 AKSRLIQTAVLLHPSFVSLDDIKGVDIPIAILGAEVDQVSPPELVKQFEQVLAAKSGVAS 183
             +K  LIQ AVLLHPSFV++DDIKG   PIAILGAE+DQ+SPP L+KQFE++L++K  V S
Sbjct:   137 SKEELIQAAVLLHPSFVNVDDIKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNS 196

Query:   184 FVKIFPKVSHGWAVRYNTEDAETVKVAEEAHQDLLDWLAKHHK 312
             +VKI PKVSHGW VRYN ++ E VK AEEAH+++LDW   + K
Sbjct:   197 YVKIHPKVSHGWTVRYNIDEPEAVKAAEEAHKEMLDWFVTYIK 239


to_Entrezto_Relatedto_Related >gi|9294513|dbj|BAB02775.1|  (AB023036) contains similarity to
            endo-1,3-1,4-beta-D-glucanase~gene_id:MDB19.5 [Arabidopsis
            thaliana]
            Length = 232

Frame  1 hits (HSPs):                              _______________________
                        __________________________________________________
Database sequence:     |          |          |          |         |       | 232
                       0         50        100        150       200

  Plus Strand HSPs:

 Score = 335 (117.9 bits), Expect = 2.4e-29, P = 2.4e-29
 Identities = 62/103 (60%), Positives = 79/103 (76%), Frame = +1

Query:     4 AKSRLIQTAVLLHPSFVSLDDIKGVDIPIAILGAEVDQVSPPELVKQFEQVLAAKSGVAS 183
             AK +L+   VLLHP+ V++DDIK V++PIA+LGAE+DQVSPPELV+QFE +LA+K  V S
Sbjct:   130 AKEKLVDATVLLHPARVTVDDIKEVNLPIAVLGAEIDQVSPPELVRQFEDILASKPQVKS 189

Query:   184 FVKIFPKVSHGWAVRYNTEDAETVKVAEEAHQDLLDWLAKHHK 312
             FVKIFP+  HGW VRYN  D   V+ A EAH+D+L WL  + K
Sbjct:   190 FVKIFPRCKHGWTVRYNENDPSEVEAAMEAHKDMLAWLIDYLK 232


to_Entrezto_Related >gi|6319267  ref|NP_009350.1| Yal049cp [Saccharomyces cerevisiae]
            >gi|731285|sp|P39721|YAE9_YEAST HYPOTHETICAL 27.1 KD PROTEIN IN
            ACS1-GCV3 INTERGENIC REGION >gi|1077483|pir||S51970 hypothetical
            protein YAL049c - yeast (Saccharomyces cerevisiae)
            >gi|595535|gb|AAC04982.1| (U12980) Yal049cp [Saccharomyces
            cerevisiae]
            Length = 246

Frame  1 hits (HSPs):                                ___________________  
                        __________________________________________________
Database sequence:     |         |          |         |         |         | 246
                       0        50        100       150       200

  Plus Strand HSPs:

 Score = 114 (40.1 bits), Expect = 0.00048, P = 0.00048
 Identities = 35/92 (38%), Positives = 52/92 (56%), Frame = +1

Query:    16 LIQTAVLLHPSFVSLDDIKGVDI--PIAILGAEVDQVSPPELVKQFEQVLAAKSGVASF- 186
             L   A + HPSFVS+++I+ +D   PI I  AE D + P  L    E+ L  K   A++ 
Sbjct:   146 LANAAAIAHPSFVSIEEIEAIDSKKPILISAAEEDHIFPANLRHLTEEKL--KDNHATYQ 203

Query:   187 VKIFPKVSHGWAVRYNTEDAETVKVAEEAHQDLLD 291
             + +F  V+HG+A R +      VK A+E  + LLD
Sbjct:   204 LDLFSGVAHGFAARGDIS-IPAVKYAKE--KVLLD 235


to_Entrezto_Relatedto_Related >gi|8928122|sp|Q9ZT66|E134_MAIZE  ENDO-1,3;1,4-BETA-D-GLUCANASE PRECURSOR
            >gi|3822036|gb|AAC69757.1| (AF072326) endo-1,3-1,4-beta-D-glucanase
            [Zea mays]
            Length = 303

Frame  1 hits (HSPs):                             __________              
                        __________________________________________________
Database sequence:     |        |       |       |       |        |       || 303
                       0       50     100     150     200      250     300

  Plus Strand HSPs:

 Score = 98 (34.5 bits), Expect = 0.079, P = 0.076
 Identities = 23/57 (40%), Positives = 33/57 (57%), Frame = +1

Query:     7 KSRLIQTAVLLHPSFVSLDDIKGVDIPIAILGAEVDQVSPPELVKQFEQVLAAKSGV 177
             K+  ++   L HP  V+ DD+K V  PI ILGA+ D  +PP+ V +F  VL  +  V
Sbjct:   163 KTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEV 219


to_Entrezto_Related >gi|11498723  ref|NP_069952.1| hypothetical protein [Archaeoglobus fulgidus]
            >gi|7483554|pir||B69390 hypothetical protein AF1123 - Archaeoglobus
            fulgidus >gi|2649469|gb|AAB90124.1| (AE001026) A. fulgidus
            predicted coding region AF1123 [Archaeoglobus fulgidus]
            Length = 71

Frame  1 hits (HSPs):   ________________________                          
                        __________________________________________________
Database sequence:     |             |             |             |        | 71
                       0            20            40            60

  Plus Strand HSPs:

 Score = 71 (25.0 bits), Expect = 0.58, P = 0.44
 Identities = 12/34 (35%), Positives = 22/34 (64%), Frame = +1

Query:    67 IKGVDIPIAILGAEVDQVSPPELVKQFEQVLAAK 168
             +K + +P+ +L AE D ++PPE V  F + + +K
Sbjct:     1 MKNIKMPVLVLAAEKDHITPPESVTAFFEKIPSK 34


to_Entrezto_Relatedto_Related >gi|11359146|pir||T50286  hypothetical protein SPAC977.15 [imported] - fission
            yeast  (Schizosaccharomyces pombe) >gi|6742164|emb|CAB69637.1|
            (AL137130) hypothetical protein [Schizosaccharomyces pombe]
            Length = 247

Frame  1 hits (HSPs):                                ____________________ 
                        __________________________________________________
Database sequence:     |         |          |         |         |         | 247
                       0        50        100       150       200

  Plus Strand HSPs:

 Score = 80 (28.2 bits), Expect = 7.6, P = 1.0
 Identities = 28/94 (29%), Positives = 44/94 (46%), Frame = +1

Query:     7 KSRLIQTAVLLHPSFVSLDDIKGVDIPIAILGAEVDQVSPPELVKQFEQVLAAKSGVA-S 183
             K R ++     HPS +   D K V  P+  L ++ D+   PE V  +++        + S
Sbjct:   149 KERFLRIGCA-HPSLLDPVDAKHVHCPVCFLCSK-DE--DPEEVDAWKKSFENSPYFSES 204

Query:   184 FVKIFPKVSHGW-AVRYNTEDAETVKVAEEAHQDLL 288
             + + F K+ HGW A R N  D E  K  +  +Q  L
Sbjct:   205 YFETFGKMHHGWMAARANLSDPENRKYFDLGYQIFL 240


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.99

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.364   0.163   0.667  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.371   0.168   0.655  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.345   0.152   0.508  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.351   0.155   0.534  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.349   0.155   0.551  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.334   0.137   0.435  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      183       182       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   +2      0      184       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   +1      0      184       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -1      0      184       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -2      0      184       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -3      0      183       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  6
  No. of states in DFA:  597 (59 KB)
  Total size of DFA:  220 KB (256 KB)
  Time to generate neighborhood:  0.01u 0.00s 0.01t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  178.66u 1.02s 179.68t  Elapsed: 00:00:31
  Total cpu time:  178.69u 1.04s 179.73t  Elapsed: 00:00:31
  Start:  Sat Feb  2 04:58:08 2002   End:  Sat Feb  2 04:58:39 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000