|
| Status |
Public on May 01, 2008 |
| Title |
WT_Doxorubicinvs.WT_4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source Name |
wildtype mice
|
| Organism(s) |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: male
age: 10 months
tissue: whole heart
|
| Biomaterial provider |
Cecilia Hertig, INGEBI-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires
|
| Treatment protocol |
No treatment was applied.
|
| Growth protocol |
Animals were kept in a SPF barrier under standardisized conditions with respect to temperature and humidity, and were housed on a 12h light/12h dark cycle in groups of 4-5 with free access to food and water. Animal housing, care, and applications of experimental procedures complied with the Guide for the Care and Use of Laboratory Animals of the Government of Berlin, Germany and are in accordance to the recommendations of the Society for Laboratory Animal Science (GV-SOLAS) and the Federation of European Laboratory Animal Science Associations (FELASA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol Reagent (Invitrogen) according to manufacturer`s instructions.
|
| Label |
Cy3
|
| Label protocol |
Labeling of the targets during reverse transcription was performed as described by Pat Brown (http://brownlab.stanford.edu/).
|
| |
|
| Channel 2 |
| Source Name |
wildtype mice treated with Doxorubicin
|
| Organism(s) |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: male
age: 10 months
tissue: whole heart
|
| Biomaterial provider |
Cecilia Hertig, INGEBI-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires
|
| Treatment protocol |
Wildtype mice were treated 3 times a week with 15mg/kg Doxorubicin.
|
| Growth protocol |
Animals were kept in a SPF barrier under standardisized conditions with respect to temperature and humidity, and were housed on a 12h light/12h dark cycle in groups of 4-5 with free access to food and water. Animal housing, care, and applications of experimental procedures complied with the Guide for the Care and Use of Laboratory Animals of the Government of Berlin, Germany and are in accordance to the recommendations of the Society for Laboratory Animal Science (GV-SOLAS) and the Federation of European Laboratory Animal Science Associations (FELASA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol Reagent (Invitrogen) according to manufacturer`s instructions.
|
| Label |
Cy5
|
| Label protocol |
Labeling of the targets during reverse transcription was performed as described by Pat Brown (http://brownlab.stanford.edu/).
|
| |
|
| |
| Hybridization protocol |
Targets were vacuum-centrifuged and resuspended in 50 μl DIG hybridisation buffer (Roche). Denatured targets (98°C, 2 min) were hybridised in a humidified chamber at 42°C overnight. The following day arrays were washed (2xSSC; 0,03% SDS for 10min, 2xSSC for 5min, 1xSSC for 5min, 0,2 x SSC for 2min) and dried in a centrifuge.
|
| Scan protocol |
Agilent Microarray scanner (G2565BA)
|
| Description |
The gene expression of the whole heart of Doxorubicin treated wildtype mice was compared with wildtype mice.
|
| Data processing |
Local background was subtracted and LOWESS normalisation was performed using Feature Extraction Software (version A 6.1.1).
|
| |
|
| Submission date |
May 07, 2007 |
| Contact name |
Henning Witt |
| E-mail(s) |
witt@molgen.mpg.de
|
| Phone |
++49-30-450578727
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Hans Lehrach
|
| Lab |
Patricia Ruiz
|
| Street address |
Ihnestrasse 65
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL871 |
| Series (1) |
| GSE7746 |
Gene expression profiling on mouse models of dilated cardiomyopathy |
|
