BLASTP 2.2.17 [Aug-26-2007]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schäffer, Alejandro A., L. Aravind, Thomas L. Madden,
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= MII0006
(246 letters)
Database: nr
5,470,121 sequences; 1,894,087,724 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|4322368|gb|AAD16031.1| membrane spanning protein MutE [S... 389 e-106
gi|38257114|ref|NP_940776.1| NukE [Staphylococcus warneri] ... 84 9e-15
gi|24379580|ref|NP_721535.1| putative transporter, trans-me... 65 3e-09
gi|2581774|gb|AAC72254.1| LctE [Lactococcus lactis subsp. l... 64 5e-09
gi|90962354|ref|YP_536270.1| ABC transporter [Lactobacillus... 63 2e-08
gi|118477350|ref|YP_894501.1| possible ABC transporter, per... 60 9e-08
gi|145955513|ref|ZP_01804514.1| hypothetical protein CdifQ_... 57 9e-07
gi|126698245|ref|YP_001087142.1| putative lantibiotic ABC t... 57 1e-06
gi|47569991|ref|ZP_00240654.1| cdd3 [Bacillus cereus G9241]... 57 1e-06
gi|1770139|emb|CAA63568.1| cdd3 [Clostridium difficile] 57 1e-06
>gi|4322368|gb|AAD16031.1| membrane spanning protein MutE [Streptococcus mutans]
Length = 246
Score = 389 bits (998), Expect = e-106, Method: Composition-based stats.
Identities = 246/246 (100%), Positives = 246/246 (100%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDIFQILIFNLYTINNL 60
MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDIFQILIFNLYTINNL
Sbjct: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDIFQILIFNLYTINNL 60
Query: 61 IIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFTF 120
IIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFTF
Sbjct: 61 IIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFTF 120
Query: 121 ISSYQYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLSMLT 180
ISSYQYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLSMLT
Sbjct: 121 ISSYQYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLSMLT 180
Query: 181 SGVLAKQITIFIPWEYLSTLNPYTINDSGIHLSANYPLYFSLVILIHLFLLVTSIWLVFK 240
SGVLAKQITIFIPWEYLSTLNPYTINDSGIHLSANYPLYFSLVILIHLFLLVTSIWLVFK
Sbjct: 181 SGVLAKQITIFIPWEYLSTLNPYTINDSGIHLSANYPLYFSLVILIHLFLLVTSIWLVFK 240
Query: 241 ERVIHE 246
ERVIHE
Sbjct: 241 ERVIHE 246
>gi|38257114|ref|NP_940776.1| NukE [Staphylococcus warneri]
gi|37572673|dbj|BAC98763.1| NukE [Staphylococcus warneri]
gi|38142407|dbj|BAD01011.1| NukE [Staphylococcus warneri]
Length = 250
Score = 83.6 bits (205), Expect = 9e-15, Method: Composition-based stats.
Identities = 69/245 (28%), Positives = 131/245 (53%), Gaps = 13/245 (5%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYW-CSVS-NNLQINKNSSDIFQILIFNLYTIN 58
++ + E K RR + LPIL + I W C+++ L I I+ +LI N+ TI+
Sbjct: 2 LKAINLEFFKMRRRKFILPILLITFVGILWFCAIAIKELNITDTKYGIY-MLISNILTID 60
Query: 59 NLIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIF 118
++I P +L SR+ I+ + + + +L T+ ++ LF SK + L+ILF+ + QL
Sbjct: 61 SMIYPILIGILCSRLADIEHQGKTFQLLNTSKQSVFNLFTSKVGVSLIILFVIDIIQLTT 120
Query: 119 TFISSYQYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLSM 178
+ + ++ + F+++ ++ S ++LI + LSF + + IV L GSFL +
Sbjct: 121 ILLIANSHNISLKLEIISKFMLSFIIASFFLILIHMALSFFFEKQSVSIVSALVGSFLGL 180
Query: 179 LTSGVLAKQITIFIPWEYLSTLNPYTINDSGIHLSANYP--------LYFSLVILIHLFL 230
+T G+L I IF+PW+Y S LNP ++ +H Y ++ S+++++ LF
Sbjct: 181 VTGGMLPSYIKIFLPWQYYSLLNP--VHKKMVHKGYEYSNNDYYFIYIFISILVIVILFF 238
Query: 231 LVTSI 235
++ ++
Sbjct: 239 IIKAL 243
>gi|24379580|ref|NP_721535.1| putative transporter, trans-membrane domain bacteriocin immunity
protein [Streptococcus mutans UA159]
gi|24377526|gb|AAN58841.1|AE014951_12 putative transporter, trans-membrane domain bacteriocin immunity
protein [Streptococcus mutans UA159]
Length = 246
Score = 65.1 bits (157), Expect = 3e-09, Method: Composition-based stats.
Identities = 55/200 (27%), Positives = 100/200 (50%), Gaps = 2/200 (1%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDIFQILIFNLYTINNL 60
M L E K +R L ++ + + + W SV N ++ N+ + + IF++ IN+L
Sbjct: 1 MLNLGLEFTKLKRRSFLLSLIVIIIVELVWISVVINHEL-ANTHEAYNA-IFDMAYINDL 58
Query: 61 IIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFTF 120
I+P ++LASR++ ++ + + +L T+ E+ LF +K +L + F+ Q IF
Sbjct: 59 ILPLFISVLASRLLEMEHLGKTFKLLQTSNESPWQLFKAKLILMTIFTFVVSWLQTIFLK 118
Query: 121 ISSYQYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLSMLT 180
+ + L ++T L + + L L+F+ Q + + +V GL GSFLS +
Sbjct: 119 FIIQAAQVKVTPAQLSLSLLTTFLVCIFLSCLHLCLAFIYQKANVTVVTGLIGSFLSFVG 178
Query: 181 SGVLAKQITIFIPWEYLSTL 200
L I F+PW+Y S +
Sbjct: 179 ISALPLPIRFFVPWQYFSMM 198
>gi|2581774|gb|AAC72254.1| LctE [Lactococcus lactis subsp. lactis]
Length = 250
Score = 64.3 bits (155), Expect = 5e-09, Method: Composition-based stats.
Identities = 51/203 (25%), Positives = 102/203 (50%), Gaps = 1/203 (0%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQIN-KNSSDIFQILIFNLYTINN 59
++ + E+ K +R R + P++ F+ + W SV + S D ILI +L IN+
Sbjct: 2 LKYIYLELLKTKRRRFFSPLIIFFVVGLLWSSVLFVKEYPLPYSGDKIVILINDLTIINS 61
Query: 60 LIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFT 119
+ P ++ S + ++ N+ + VL TNG++ LF +K + LI+ I + Q +
Sbjct: 62 MFSPLIIGIMCSNLFSLEHDNQTFKVLQTNGQSIENLFYAKTIFAFLIILIFGLFQFFYI 121
Query: 120 FISSYQYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLSML 179
+ + + L+ I ++ S ++++ L +S I+ + I+I + SF+ ++
Sbjct: 122 NLIAILFKIGDPIYLSFLYFIGYIIASLFLIILHLFISLKIRKQSLGIIISVIASFVGLV 181
Query: 180 TSGVLAKQITIFIPWEYLSTLNP 202
+ G+L + IPW+Y S L+P
Sbjct: 182 SGGMLPLYLKFIIPWQYFSLLSP 204
>gi|90962354|ref|YP_536270.1| ABC transporter [Lactobacillus salivarius subsp. salivarius UCC118]
gi|90821548|gb|ABE00187.1| ABC transporter [Lactobacillus salivarius subsp. salivarius UCC118]
Length = 246
Score = 62.8 bits (151), Expect = 2e-08, Method: Composition-based stats.
Identities = 52/200 (26%), Positives = 91/200 (45%), Gaps = 2/200 (1%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDIFQILIFNLYTINNL 60
M L+ E K +R L ++ + + W V ++ K I + + +N+
Sbjct: 1 MLNLQLEFIKLKRRSFLLSLIAIVAVGLIWSGVVIKYELPKTHEAYNAI--YTMTYLNDC 58
Query: 61 IIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFTF 120
I+P A+LASR++ ++ + + +L T+ E+ LF +K + + F+ + Q +F
Sbjct: 59 ILPLFIAVLASRLLELEHLGKTFKLLQTSNESPWQLFKAKLTVMAIFAFVVSLIQTLFLK 118
Query: 121 ISSYQYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLSMLT 180
+ LF T L + + L L+F+ + +V GL GSFLS +
Sbjct: 119 FIIQGMQVSVTPVSLSLFWFTTFLVCLFLSCLHLCLAFIYPKPSVTVVTGLIGSFLSFVG 178
Query: 181 SGVLAKQITIFIPWEYLSTL 200
G L I IFIPW+Y S +
Sbjct: 179 IGALPFPIRIFIPWQYFSMM 198
>gi|118477350|ref|YP_894501.1| possible ABC transporter, permease [Bacillus thuringiensis str. Al
Hakam]
gi|118416575|gb|ABK84994.1| possible ABC transporter, permease [Bacillus thuringiensis str. Al
Hakam]
Length = 250
Score = 60.5 bits (145), Expect = 9e-08, Method: Composition-based stats.
Identities = 50/207 (24%), Positives = 99/207 (47%), Gaps = 4/207 (1%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSD-IFQILIFNLYTINN 59
++ L E K RR ++W+ I+ + + W +S + I N + +++ + F++ ++N
Sbjct: 2 IKLLGLEYFKIRRKKIWIMIILFLIVEMLWAFMSISRSIASNPDNRVWEAIFFSISSMNG 61
Query: 60 LIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFT 119
L +P +A++ASR+ ++ K + +L L+ +KY+ +L G Q++F
Sbjct: 62 LFMPIISAVVASRICDMEHKGSTWKMLAATNVERGQLYAAKYICINSLLLYGIFAQVLFI 121
Query: 120 FISSY--QYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLS 177
+ + I I FI LL + + +Q +SF I++ + +G+ G F+
Sbjct: 122 IVFGLINDFPGTIPIGLLIRFIGGNLLTTLAVTALQQWISFSIKNQSFALCLGMLGGFIG 181
Query: 178 MLTSGVLAKQITIFIPWEYLSTLNPYT 204
M T+G+ I W Y L+P T
Sbjct: 182 M-TAGLFPAAIRHIFIWSYYLELSPVT 207
>gi|145955513|ref|ZP_01804514.1| hypothetical protein CdifQ_04000711 [Clostridium difficile
QCD-32g58]
Length = 253
Score = 57.0 bits (136), Expect = 9e-07, Method: Composition-based stats.
Identities = 56/252 (22%), Positives = 122/252 (48%), Gaps = 7/252 (2%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDI----FQILIFNLYT 56
M+ L E K +R ++ L I WC V++N I +N + + ++ + +
Sbjct: 1 MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPDMLGGFEYSYMLMSFAS 60
Query: 57 INNLIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQL 116
+N L P +++ SR+ I+ K + +L ++ + ++LSK++ +++ I + Q+
Sbjct: 61 LNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYLSKFLCSAILVSIPILMQV 120
Query: 117 IFTFISSYQYHAI--INKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGS 174
+ + + I I+ + + + + +L S I+ + L ++ VI + +I I G+ GS
Sbjct: 121 LSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHLWIATVIPNQMIAITFGMIGS 180
Query: 175 FLSMLTSGVLAKQITIFIPWEYLSTLNPYTINDSGIHLSANYPLYFSLVILIHLFLLVTS 234
F+ LTSG+ + I W Y L+P + + S+ Y + + I I +FL+
Sbjct: 181 FIG-LTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGSSVYRIDMNFSITILVFLIGML 239
Query: 235 IWLVFKERVIHE 246
I+ + + ++ +
Sbjct: 240 IYYIGRNHLLKK 251
>gi|126698245|ref|YP_001087142.1| putative lantibiotic ABC transporter, peremase protein [Clostridium
difficile 630]
gi|115249682|emb|CAJ67499.1| putative lantibiotic ABC transporter, peremase protein [Clostridium
difficile 630]
Length = 253
Score = 57.0 bits (136), Expect = 1e-06, Method: Composition-based stats.
Identities = 56/252 (22%), Positives = 122/252 (48%), Gaps = 7/252 (2%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDI----FQILIFNLYT 56
M+ L E K +R ++ L I WC V++N I +N + + ++ + +
Sbjct: 1 MKYLGLEFYKLKRKKIILMTFLFVTVEIMWCIVNSNRAITRNPDMLGGFEYSYMLMSFAS 60
Query: 57 INNLIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQL 116
+N L P +++ SR+ I+ K + +L ++ + ++LSK++ +++ I + Q+
Sbjct: 61 LNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYLSKFLCSAILVSIPVLMQV 120
Query: 117 IFTFISSYQYHAI--INKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGS 174
+ + + I I+ + + + + +L S I+ + L ++ VI + +I I G+ GS
Sbjct: 121 LSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHLWIATVIPNQMIAITFGMIGS 180
Query: 175 FLSMLTSGVLAKQITIFIPWEYLSTLNPYTINDSGIHLSANYPLYFSLVILIHLFLLVTS 234
F+ LTSG+ + I W Y L+P + + S+ Y + + I I +FL+
Sbjct: 181 FIG-LTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGSSVYRIDMNFSITILVFLIGML 239
Query: 235 IWLVFKERVIHE 246
I+ + + ++ +
Sbjct: 240 IYYIGRNHLLKK 251
>gi|47569991|ref|ZP_00240654.1| cdd3 [Bacillus cereus G9241]
gi|47553339|gb|EAL11727.1| cdd3 [Bacillus cereus G9241]
Length = 250
Score = 56.6 bits (135), Expect = 1e-06, Method: Composition-based stats.
Identities = 48/207 (23%), Positives = 96/207 (46%), Gaps = 4/207 (1%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSD-IFQILIFNLYTINN 59
++ L E K RR ++W I+ + + W +S + I N + +++ + F++ ++N
Sbjct: 2 IKILALEYFKIRRKKIWTMIILFLIVEMLWAFMSISRSIASNPDNRVWESIFFSISSMNG 61
Query: 60 LIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIFT 119
L +P +A++ASR+ ++ K + +L L+ +KY+ ++ Q++F
Sbjct: 62 LFMPIISAVVASRICDMEHKGSTWKMLVATNVEHRQLYAAKYICINSLMLNAIFAQILFI 121
Query: 120 FISSY--QYHAIINKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGSFLS 177
+ + + FI LL + I IQ +SF I++ + +G+ G F+
Sbjct: 122 IVFGLIKNFPGTVPIELLTRFIFGNLLTTLAITAIQQWVSFSIKNQSFALCLGMLGGFIG 181
Query: 178 MLTSGVLAKQITIFIPWEYLSTLNPYT 204
M T+G+ I W Y L+P T
Sbjct: 182 M-TAGLFPAAIRHIFIWSYYLELSPVT 207
>gi|1770139|emb|CAA63568.1| cdd3 [Clostridium difficile]
Length = 253
Score = 56.6 bits (135), Expect = 1e-06, Method: Composition-based stats.
Identities = 56/252 (22%), Positives = 122/252 (48%), Gaps = 7/252 (2%)
Query: 1 MRQLRSEIQKYRRNRLWLPILGLFLFSIYWCSVSNNLQINKNSSDI----FQILIFNLYT 56
M+ L E K +R ++ L I WC V++N I +N + + ++ + +
Sbjct: 1 MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPDMLGGFEYSYMLMSFAS 60
Query: 57 INNLIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQL 116
+N L P +++ SR+ I+ K + +L ++ + ++LSK++ +++ I + Q+
Sbjct: 61 LNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNTIYLSKFLCSAILVSIPVLMQV 120
Query: 117 IFTFISSYQYHAI--INKNEFILFIITMLLGSGTILLIQLLLSFVIQSSIIPIVIGLAGS 174
+ + + I I+ + + + + +L S I+ + L ++ VI + +I I G+ GS
Sbjct: 121 LSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHLWIATVIPNQMIAITFGMIGS 180
Query: 175 FLSMLTSGVLAKQITIFIPWEYLSTLNPYTINDSGIHLSANYPLYFSLVILIHLFLLVTS 234
F+ LTSG+ + I W Y L+P + + S+ Y + + I I +FL+
Sbjct: 181 FIG-LTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGSSVYRIDMNFSITILVFLIGML 239
Query: 235 IWLVFKERVIHE 246
I+ + + ++ +
Sbjct: 240 IYYIGRNHLLKK 251
Database: nr
Posted date: Sep 17, 2007 11:41 AM
Number of letters in database: 999,999,834
Number of sequences in database: 2,976,859
Database: /nucleus1/users/jsaw/ncbi/db/nr.01
Posted date: Sep 17, 2007 11:48 AM
Number of letters in database: 894,087,890
Number of sequences in database: 2,493,262
Lambda K H
0.332 0.146 0.433
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 829,800,314
Number of Sequences: 5470121
Number of extensions: 33324177
Number of successful extensions: 137247
Number of sequences better than 1.0e-05: 18
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 137225
Number of HSP's gapped (non-prelim): 18
length of query: 246
length of database: 1,894,087,724
effective HSP length: 129
effective length of query: 117
effective length of database: 1,188,442,115
effective search space: 139047727455
effective search space used: 139047727455
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 128 (53.9 bits)