|
| Status |
Public on Jan 10, 2009 |
| Title |
retina_hypox_2h_2 |
| Sample type |
RNA |
| |
|
| Source Name |
retina after 2 h hypoxic treatment
|
| Organism(s) |
Mus musculus |
| Characteristics |
retina
BALB/c
|
| Treatment protocol |
After hypoxic preconditioning (6% O2 for 6 hours) reoxygenation was allowed in darkness for 0 h, 2 h, 4 h, 12 h and 16 h in normal room air in darkness. After reoxygenation BALB/c mice were exposed to 5'000 lux of white fluorescent light for 1 h and analyzed at the time points as indicated (Thiersch et al, BMC Genomics. 2008, 9:73).
|
| Growth protocol |
BALB/c mice were grown under standard conditons (ARVO).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinas were isolated immediately, 0 h, 2 h, 4 h and 16 h after hypoxic preconditioning, frozen in liquid nitrogen and stored at -70°C, 3 retinas of 3 different mice were pooled and the procedure repeated 3 times to generate independent biological triplicates. RNA was extracted using the RNeasy isolation kit (Qiagen, Hilden, Germany), including a DNase treatment to digest residual genomic DNA.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from the total RNA according to the Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2007, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2007, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner.
|
| Description |
retina after 2 h hypoxic treatment
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jan 09, 2009 |
| Contact name |
Wolfgang Raffelsberger |
| E-mail(s) |
wolfgang.raffelsberger@igbmc.fr
|
| URL |
http://www-bio3d-igbmc.u-strasbg.fr/~wraff
|
| Organization name |
IGBMC
|
| Department |
DBGS
|
| Lab |
LBGI
|
| Street address |
1 rue Laurent Fries
|
| City |
Illkirch/Strasbourg |
| ZIP/Postal code |
67404 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14366 |
Analysis of the retinal gene expression after hypoxic preconditioning identifies candidate genes for neuroprotection |
|
