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Quantity of HIV positive blood and viability of lymphomononuclear cells transferred to skin and "interstitial" fluid following needle stick: an in vitro model.

Shirazian D, Spatoliatore G, Mokhtarian F, Herzlich B, Grob D; International Conference on AIDS.

Int Conf AIDS. 1991 Jun 16-21; 7: 312 (abstract no. M.C.3056).

Maimonides Medical Center, SUNY Health Science Center at Brooklyn, NY USA

OBJECTIVE: We investigated the quantity of blood and viability of lymphomononuclear cells transmitted following needle stick as these factors are important in affecting HIV seroconversion in health care workers. METHODS: A model consisting of human, mouse, and mock skin covering "interstitial" fluid was developed. We measured quantity of HIV positive blood (using modified Durkin's method and Cr 51 labeled RBCs) transferred to skin and fluid following needle stick puncture from 20-27 gauge needles and viability of lymphomononuclear cells transferred to fluid using cultivation by limiting dilution. RESULTS: Using human skin, 0.198 +/- 62 mul (microliters) to .004 +/- 5 mul (measured by Durkin's method) and 0.233 +/- 119 mul to .0035 +/- 4.5 mul (measured by Cr 51 labeled RBCs) were transferred by 20-27 g needle sticks to fluid. Human skin reduced the volume of blood transmitted to fluid by 71% when 22 gauge needles were used. Viable lymphocytes were transmitted to fluid in 60% of experiments. DISCUSSION AND CONCLUSIONS: Approximately 0.3 to 0.004 microliters of blood and viable lymphomononuclear cells are transmitted from 20-27 gauge needles to interstitial fluid following needle stick. This contrasts to the previous reported quantity of 1.4 microliters. We are presently investigating transmission of HIV to skin and fluid from a variety of HIV infected specimens in order to assess the importance of the various factors in contributing to the seroconversion following a needle stick injury.

Publication Types:
  • Meeting Abstracts
Keywords:
  • Acquired Immunodeficiency Syndrome
  • Animals
  • Extracellular Fluid
  • HIV Infections
  • HIV Seropositivity
  • Health Personnel
  • Humans
  • In Vitro
  • Mice
  • Needles
  • Needlestick Injuries
  • Skin
  • blood
  • organization & administration
Other ID:
  • 1305691
UI: 102183443

From Meeting Abstracts




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