RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Rex Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Errex zachirus
FDA Seafood List Common Name: Rex Sole
FDA Seafood List Market Name: Sole (Flounder)
Unique IEF Gel Code: 19971126gel03
Unique Web Code: rxh01Srr
Tissue Aliquot Code: errezach 001 #3 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	41	5.1	0.142242	4	1.01304	10	7.4	4.55	42	4.5	0.127909
2	5.2	129	16	0.151273	4	0.977201	9.7	7.2	5.2	129.2	3.9	0.140843
3	5.85	231	28.7	0.199332	3	1.19229	11.8	8.8	5.85	231.1	3.4	0.19692
4	6.55	384	47.8	0.174763	7	1.77166	17.5	13	6.55	382	6.6	0.152045
5	6.85	416	51.7	0.0462949	5	0.401014	4	2.9	6.85	416.4	5.4	0.041923
6	7.35	492	61.2	0.104595	5	0.811615	8	6	7.35	491.5	4.7	0.0972382
7	8.15	616	76.6	0.0879376	8	1.13073	11.2	8.3	8.15	616.1	7.8	0.0820553
8	8.45	681	84.7	0.125589	7	1.41975	14	10.4	8.45	680.6	6.5	0.122969
9	8.65	733	91.2	0.114145	4	0.752435	7.4	5.5	8.65	732.5	4	0.10715
10	9.3	784	97.5	0.0848406	5	0.645736	6.4	4.7	9.3	783.4	4.5	0.0809047

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.382	20	2.5	0.427093	5	3.35323	13.7	11.2	4.382	20.9	4.6	0.411986
2	4.665	57	7.1	0.23037	20	7.59303	31	25.4	4.665	62.7	20.3	0.211435
3	5.3	143	17.8	0.0156674	4	0.0949002	0.4	0.3	5.3	142.6	3.5	0.0151937
4	5.363	153	19	0.435167	3	2.0008	8.2	6.7	5.363	153.3	2.5	0.446626
5	5.407	160	19.9	0.378073	3	2.1455	8.8	7.2	5.407	160.4	3.2	0.38313
6	5.552	183	22.8	0.240978	4	1.52476	6.2	5.1	5.552	182.7	4	0.21528
7	5.709	208	25.9	0.0720612	18	1.85638	7.6	6.2	5.709	199.6	18.2	0.0575182
8	5.815	225	28	0.120174	3	0.640494	2.6	2.1	5.815	225	3	0.122073
9	6.239	317	39.4	0.185082	9	2.61041	10.6	8.7	6.239	316.6	8.8	0.167234
10	6.307	332	41.3	0.165808	9	2.59199	10.6	8.7	6.307	333.3	9.3	0.157755
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.446	363	45.1	0.0194902	3	0.105689	0.4	0.4	6.446	362.9	3.4	0.017798

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	43	5.3	0.127934	6	1.10574	9.9	7.4	4.55	44.2	5.8	0.108381
2	5.2	125	15.5	0.131077	4	0.855063	7.6	5.8	5.2	125.4	3.9	0.123349
3	5.85	230	28.6	0.187799	3	1.13962	10.2	7.7	5.85	230.4	3.4	0.189793
4	6.55	388	48.3	0.192512	6	1.74607	15.6	11.7	6.55	386.4	5.7	0.172882
5	6.85	419	52.1	0.0492667	5	0.428203	3.8	2.9	6.85	419.2	5.5	0.043951
6	7.35	493	61.3	0.134612	5	1.01092	9	6.8	7.35	492.8	4.6	0.125022
7	8.15	622	77.4	0.120588	8	1.59986	14.3	10.8	8.15	621.5	8	0.112799
8	8.45	682	84.8	0.149149	7	1.73031	15.4	11.6	8.45	681	6.7	0.145004
9	8.65	732	91	0.125856	4	0.886373	7.9	6	8.65	732	4.1	0.122346
10	9.3	781	97.1	0.08715	5	0.705688	6.3	4.7	9.3	780.6	5.1	0.0775696

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.362	19	2.4	0.392205	5	2.98966	13.7	10.6	4.362	19.3	4.5	0.374056
2	4.55	43	5.3	0.0973316	1	0.00470158	0	0	4.55	42.6	1.2	0.00218336
3	4.66	57	7.1	0.187225	18	5.37972	24.6	19	4.66	60.6	18	0.168624
4	5.2	126	15.7	0.0530981	3	0.308544	1.4	1.1	5.2	126.3	3.3	0.0531809
5	5.298	142	17.7	0.011768	2	0.0437292	0.2	0.2	5.298	142.2	1.8	0.0135877
6	5.359	152	18.9	0.440312	2	1.87931	8.6	6.6	5.359	152.2	2.4	0.438447
7	5.402	159	19.8	0.37844	3	1.86397	8.5	6.6	5.402	159.3	2.9	0.360922
8	5.543	182	22.6	0.211454	4	1.40796	6.4	5	5.543	182.1	3.9	0.201734
9	5.703	208	25.9	0.0395746	2	0.145682	0.7	0.5	5.703	208.3	2.1	0.0387599
10	5.807	225	28	0.149295	2	0.601378	2.8	2.1	5.807	224.6	2.2	0.157287
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	232	28.9	0.0798958	3	0.464451	2.1	1.6	5.85	231.6	3.3	0.0802738
12	6.252	321	39.9	0.188094	7	2.00322	9.2	7.1	6.252	321.3	7.3	0.15524
13	6.329	338	42	0.143669	10	2.37069	10.8	8.4	6.329	339.1	9.5	0.140071
14	6.469	369	45.9	0.0517714	3	0.296771	1.4	1	6.469	368.9	3.3	0.0510189
15	6.55	387	48.1	0.00946073	5	0.0894852	0.4	0.3	6.55	384.5	5	0.0101188
16	6.85	420	52.2	0.0151126	5	0.109064	0.5	0.4	6.85	420.4	4.8	0.0126949
17	7.35	496	61.7	0.0153637	12	0.253063	1.2	0.9	7.35	497.9	12.3	0.0116543
18	8.15	627	78	0.0288746	10	0.486211	2.2	1.7	8.15	624.2	10.2	0.0269732
19	8.45	689	85.7	0.0470542	6	0.456517	2.1	1.6	8.45	688.9	5.7	0.0454914
20	8.65	736	91.5	0.043811	4	0.323052	1.5	1.1	8.65	735.8	4.4	0.0412975
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.3	783	97.4	0.0487314	5	0.390771	1.8	1.4	9.3	782.8	4.7	0.0465835

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.382	21	2.6	0.409319	5	3.16041	15.2	11	4.382	21.7	4.6	0.390726
2	4.666	59	7.3	0.23555	18	6.92191	33.3	24.1	4.666	64.8	18	0.216963
3	5.289	145	18	0.012427	3	0.0680138	0.3	0.2	5.289	145.8	2.9	0.0133005
4	5.362	157	19.5	0.478657	2	2.03553	9.8	7.1	5.362	157	2.4	0.482216
5	5.405	164	20.4	0.406149	3	2.04134	9.8	7.1	5.405	164.6	2.8	0.40467
6	5.54	186	23.1	0.211808	4	1.40579	6.8	4.9	5.54	186.9	3.9	0.203514
7	5.694	211	26.2	0.0402023	2	0.151329	0.7	0.5	5.694	211.5	2.1	0.040835
8	5.798	228	28.4	0.159144	2	0.595527	2.9	2.1	5.798	228.6	2	0.165266
9	6.216	318	39.6	0.18141	8	2.20982	10.6	7.7	6.216	319.5	8.3	0.150411
10	6.296	336	41.8	0.123132	9	1.88401	9.1	6.6	6.296	336.8	8.9	0.119576
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.445	369	45.9	0.0219516	9	0.294884	1.4	1	6.445	372.5	9.1	0.0183202

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	44	5.5	0.135664	5	1.05292	10.1	7.3	4.55	45.2	5	0.118468
2	5.2	135	16.8	0.131171	5	1.04054	10	7.2	5.2	135.2	4.6	0.126267
3	5.85	241	30	0.168095	4	1.11946	10.7	7.8	5.85	241.2	3.9	0.162806
4	6.55	398	49.5	0.174047	6	1.77299	17	12.3	6.55	396.7	6.4	0.157231
5	6.85	426	53	0.0437547	6	0.422454	4.1	2.9	6.85	427	6.1	0.0390928
6	7.35	498	61.9	0.0894756	5	0.703225	6.7	4.9	7.35	497	4.7	0.0842418
7	8.15	624	77.6	0.103008	8	1.33279	12.8	9.3	8.15	624.2	7.7	0.0974168
8	8.45	689	85.7	0.125946	7	1.44515	13.9	10	8.45	688.3	6.6	0.12323
9	8.65	741	92.2	0.117804	4	0.774783	7.4	5.4	8.65	740.7	3.8	0.113801
10	9.3	786	97.8	0.0897218	5	0.762142	7.3	5.3	9.3	785.6	5.3	0.0818041

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 73

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 10.000 5.200 9.700 5.850 11.800 6.550 17.500 6.850 4.000 7.350 8.000 8.150 11.200 8.450 14.000 8.650 7.400 9.300 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.382 13.700 4.665 31.000 5.300 0.400 5.363 8.200 5.407 8.800 5.552 6.200 5.709 7.600 5.815 2.600 6.239 10.600 6.307 10.600 6.446 0.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 11 Return to Lane Navigator
4.550 9.900 5.200 7.600 5.850 10.200 6.550 15.600 6.850 3.800 7.350 9.000 8.150 14.300 8.450 15.400 8.650 7.900 9.300 6.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.362 13.700 4.550 0 4.660 24.600 5.200 1.400 5.298 0.200 5.359 8.600 5.402 8.500 5.543 6.400 5.703 0.700 5.807 2.800 5.850 2.100 6.252 9.200 6.329 10.800 6.469 1.400 6.550 0.400 6.850 0.500 7.350 1.200 8.150 2.200 8.450 2.100 8.650 1.500 9.300 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 21 Return to Lane Navigator
4.382 15.200 4.666 33.300 5.289 0.300 5.362 9.800 5.405 9.800 5.540 6.800 5.694 0.700 5.798 2.900 6.216 10.600 6.296 9.100 6.445 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 11 Return to Lane Navigator
4.550 10.100 5.200 10.000 5.850 10.700 6.550 17.000 6.850 4.100 7.350 6.700 8.150 12.800 8.450 13.900 8.650 7.400 9.300 7.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Rex Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Rex Sole ---- Go to Display Gel Lane Navigator.