Origin IGS: taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaccaaacatgtcggggcaaaaaacgaatatcgcgtggggtgcgcggagatgttcgatcgccgttgaagagatttgtgaagctcgcgaagactttcggatacttgacgaacgtctgcgcaagacgtatgggcatttctctcacaaaaaacaataaaaaaacagctctgtcacataagtgacatttgaagaacagacctatccggatatttgcatcatgaaattta Mask Tandem Repeat Region ================================================ taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Find is-nt database================================================ Query_seq: TF2790:TF2791|TF2790:TF2791:prismane protein, hybrid-cluster protein:GTP-binding protein TypA:->->:2975211..2975437 227 taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: TF2790:TF2791|TF2790:TF2791:prismane protein, hybrid-cluster protein:GTP-binding protein TypA:->->:2975211..2975437 227 taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: TF2790:TF2791|TF2790:TF2791:prismane protein, hybrid-cluster protein:GTP-binding protein TypA:->->:2975211..2975437 227 taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Predict ORF larger than 30AA ================================================ Protein_Len: 51 Strand: + Start: 73 End: 225 ........................................................................ M V F C E R N A H T S C A D V R Q V S E S L R E L H K S L Q R R S N I S A H P T R Y S F F A P T C L V .. Protein_Len: 60 Strand: + Start: 20 End: 226 ................... M R I G L F F K C H L C D R A V F L L F F V R E M P I R L A Q T F V K Y P K V F A S F T N L F N G D R T S P R T P R D I . Protein_Len: 41 Strand: + Start: 105 End: 227 ........................................................................................................ M R R R S S S I R K S S R A S Q I S S T A I E H L R A P H A I F V F C P D M F G L Protein_Len: 48 Strand: - Start: 74 End: 217 ......................................................................... Q K K H S F H G Y T K R L R E D L I R F D E R A E C I E E V A I S C R R A G W A I N T K Q G S M .......... Protein_Len: 50 Strand: - Start: 49 End: 198 ................................................ K H S L A T K K N N K T L S I G M R R A C V N T L Y G F T K A L K V F R K L P S R V D G R V G R S M ............................. taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: TF2790:TF2791|TF2790:TF2791:prismane protein, hybrid-cluster protein:GTP-binding protein TypA:->->:2975211..2975437 227 taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctgtttttttattgttttttgtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta Intra-Species Hit: Count: 1 Min: 1 Max: 227 Len: 227 Subject: tfor_TF2790_TF2791|prismane protein, hybrid-cluster protein:GTP-binding protein TypA|POSITIVE:POSITIVE|[2975211,2975437]|227 HSP 1 e-value: 8.0E-77 bit: 287.0 Len: 145 Query Start:83 Query End:227 Subject Strand: POSITIVE Subject Start: 83 Subject End: 227 ..................................................................................gtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta ..................................................................................gtgagagaaatgcccatacgtcttgcgcagacgttcgtcaagtatccgaaagtcttcgcgagcttcacaaatctcttcaacggcgatcgaacatctccgcgcaccccacgcgatattcgttttttgccccgacatgtttggttta HSP 2 e-value: 4.0E-29 bit: 129.0 Len: 65 Query Start:1 Query End:65 Subject Strand: POSITIVE Subject Start: 1 Subject End: 65 taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctg.................................................................................................................................................................. taaatttcatgatgcaaatatccggataggtctgttcttcaaatgtcacttatgtgacagagctg.................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUUCAUGAUGCAAAUAUCCGGAUAGGUCUGUUCUUCAAAUGUCACUUAUGUGACAGAGCUGUUUUUUUAUUGUUUUUUGUGAGAGAAAUGCCCAUACGUCUUGCGCAGACGUUCGUCAAGUAUCCGAAAGUCUUCGCGAGCUUCACAAAUCUCUUCAACGGCGAUCGAACAUCUCCGCGCACCCCACGCGAUAUUCGUUUUUUGCCCCGACAUGUUUGGUUUA ((((((.((((..(((((.(..((((((....(((((......((((((....))))))..((((((........(...((((((((....(((......)))((((((.((((.((((.........)))).)))).)))))).)))))))).).....))))))....)))))....((((.......)))).))))))..)))))).....))))...)))))) (-56.12) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACCAAACAUGUCGGGGCAAAAAACGAAUAUCGCGUGGGGUGCGCGGAGAUGUUCGAUCGCCGUUGAAGAGAUUUGUGAAGCUCGCGAAGACUUUCGGAUACUUGACGAACGUCUGCGCAAGACGUAUGGGCAUUUCUCUCACAAAAAACAAUAAAAAAACAGCUCUGUCACAUAAGUGACAUUUGAAGAACAGACCUAUCCGGAUAUUUGCAUCAUGAAAUUUA .........((((.....(((((...((((((((.((((.....))))..))))))))....(((.(((.((((..(((..(.((.(((.((((.((((.........)))).)))).))).)).....)..)))..)))))))........................((((((....))))))...................)))...)))))..))))....... (-56.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 435 215 179 217 169 tfor:TF2561|5end_50S_ribosomal_protein_L16_2738693..2738923_POSITIVE 402 200 153 56 5 tfor:TF1548|5end_thiophene_and_furan_oxidation_protein_1652501..1652731_POSITIVE 394 150 101 229 170 tfor:TF0809|5end_cation_efflux_protein_865358..865588_POSITIVE 382 144 101 151 107 tfor:TF0280|5end_hypothetical_protein_308807..309037_POSITIVE 371 130 81 173 125 tfor:TF0729|5end_DNA_primase_780107..780337_POSITIVE 368 208 163 159 111 tfor:TF0215|5end_tRNA/rRNA_methyltransferase,_TrmH_family_241463..241693_POSITIVE 365 148 101 97 45 tfor:TF2853|5end_2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase_3040172..3040402_POSITIVE 359 203 162 206 147 tfor:TF2166|5end_hypothetical_protein_2342115..2342345_POSITIVE 356 148 101 195 144 tfor:TF2786|5end_hypothetical_protein_2969851..2970081_POSITIVE 353 217 173 60 3 tfor:TF2358|5end_possible_small-conductance_mechanosensitive_channel_2523829..2524059_POSITIVE 350 148 101 69 32 tfor:TF2310|5end_dehydrogenase,_possible_3-oxoacyl-[acyl-carrier-protein]_reductase_2470481..2470711_POSITIVE 349 210 164 111 53 tfor:TF2914|5end_hypothetical_protein_3109345..3109575_POSITIVE 349 140 101 184 142 tfor:TF0953|5end_conserved_hypothetical_protein;_possible_DNA_binding_protein_997929..998159_POSITIVE 345 148 101 172 121 tfor:TF2226|5end_CTn_excision_protein_2395529..2395759_POSITIVE 344 195 154 57 11 tfor:TF0944|5end_TonB-dependent_receptor_986340..986570_POSITIVE 343 150 101 144 93 tfor:TF2517|5end_hypothetical_protein_2695089..2695319_POSITIVE 340 214 175 102 54 tfor:TF2793|5end_possible_L-lysine_2,3-aminomutase_2977661..2977891_POSITIVE 337 139 95 62 4 tfor:TF2385|5end_conserved_hypothetical_protein;_possible_MazG_family_protein_2550676..2550906_POSITIVE 335 160 113 69 8 tfor:TF3064|5end_oxidoreductase,_aldo/keto_reductase_family_3289765..3289995_POSITIVE 335 197 163 50 10 tfor:TF0038|5end_conserved_hypothetical_protein_39907..40137_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 365 148 101 66 14 tfor:TF2852|3end_conserved_hypothetical_protein_3040221..3040371_POSITIVE 347 136 93 133 92 tfor:TF1846|3end_hypothetical_protein_1997015..1997165_POSITIVE 347 136 93 123 82 tfor:TF1845|3end_hypothetical_protein_1997005..1997155_POSITIVE 338 189 141 135 82 tfor:TF1168|3end_hypothetical_protein_1249092..1249242_POSITIVE 337 139 95 62 4 tfor:TF2384|3end_thiol:disulfide_interchange_protein_2550756..2550906_POSITIVE 335 197 163 54 14 tfor:TF0037|3end_sialic_acid-specific_9-O-acetylesterase_39991..40141_POSITIVE 329 208 166 68 15 tfor:TF0705|3end_hypothetical_protein_750789..750939_POSITIVE 326 220 172 110 72 tfor:TF2547|3end_hypothetical_protein_2730947..2731097_POSITIVE 325 140 93 148 92 tfor:TF3105|3end_hypothetical_protein_3338342..3338492_POSITIVE 325 130 92 80 40 tfor:TF0603|3end_glycosyltransferase_634838..634988_POSITIVE 324 209 163 78 10 tfor:TF0599|3end_hypothetical_protein_630130..630280_POSITIVE 322 209 166 74 31 tfor:TF2159|3end_conserved_hypothetical_protein_2336076..2336226_POSITIVE 319 150 101 141 101 tfor:TF2831|3end_ABC_transporter,_permease_component_3022671..3022821_POSITIVE 318 110 63 151 94 tfor:TF1638|3end_conserved_hypothetical_protein_1747732..1747882_POSITIVE 316 189 141 57 4 tfor:TF1588|3end_hypothetical_protein_1702560..1702710_POSITIVE 316 210 162 60 7 tfor:TF0745|3end_conserved_hypothetical_protein_791074..791224_POSITIVE 315 189 141 50 8 tfor:TF2978|3end_conserved_hypothetical_protein_3190862..3191012_POSITIVE 314 147 102 113 71 tfor:TF3006|3end_response_regulator,_transcriptional_regulator_RprY_3227162..3227312_POSITIVE 314 210 164 135 80 tfor:TF1443|3end_hypothetical_protein_1528203..1528353_POSITIVE 314 143 102 151 98 tfor:TF0271|3end_oxidase/dehydrogenase_298530..298680_POSITIVE