Tamalet C, Agut H, Ferchal F, Nicholas JC, Puel J, Fleury H, Vezinet F, Rouzioux C; Conference on Retroviruses and Opportunistic Infections.
Program Abstr 3rd Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 3rd 1996 Wash D C. 1996 Jan 28-Feb 1; 3rd: 145.
ANRS, Paris,France.
Objectives: To determine whether SI ability of isolates is related to IVT and RC. Material and Methods: 46 HIV-1 infected subjects (11 greater than 500 CD4+ cells/microliter, 16 with 200-500 , 19 less than 200/microliter) have been studied using parallel quantitative cocultivation of patient PBMC and plasma with healthy donor PBMC and MT-2 cells. Fluids were tested by p24 Antigen(Ag) assay and SI detection in MT-2 cells was documented. Serum ICD p24 Ag level was determined. Results: 30 HIV-1 isolates (19 from PBMC, 11 from plasma) were obtained from 20 HIV-1 infected subjects (43%) in MT-2 cells. 20 of these isolates were SI phenotype and 14 subjects (30%) harbored SI viruses. SI phenotype and MT-2 tropism of NSI isolates were unrelated to RC (p greater than 0.05). The IVT in MT-2 cells were lower than in donor PBMC. The mean log titer (MLT) of SI viruses in donor PBMC was 0.5 log higher than that of NSI viruses (p greater than 0.05). Moreover, SI phenotype was detected despite a low IVT in PBMC. Fifty percent of AIDS patients lacked SI phenotype. IVT in MT-2 cells was similar to quantitative assessment of SI phenotype designated as SI titer. 79% of subjects with SI viruses had p24 Ag detectable level vs 38% for NSI HIV-1 infected subjects. 18/20 subjects with MT-2 tropic viruses (SI and NSI) had less than 500 CD4+ cells/microliter. Conclusion: SI phenotype :1) is unrelated to RC of isolates; 2) relationship between SI phenotype and IVT is unclear; 3) SI phenotype is an independent parameter of poor predictive value.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- CD4-Positive T-Lymphocytes
- Giant Cells
- HIV-1
- Humans
- Phenotype
- genetics
- organization & administration
Other ID:
UI: 102216550
From Meeting Abstracts