Herchenroeder O, Renne R, Mergia A, Loncar D, Murthy K, Luciw P; Symposium on Nonhuman Primate Models for AIDS.
J Med Primatol. 1993 Sep-Oct; 22: abstract no. 87.
Department of Medical Pathology, University of California, Davis 95616.
Foamy viruses, members of one of the seven genera of retroviruses, have been recovered from several species of non-human primates, and a foamy virus, designated HFV, has also been isolated from a human. In in vitro tissue culture systems, the SFVs and HFV display a wide cell tropism and cause extensive cytopathology; no disease has yet been associated with foamy viruses in indigenous hosts or in experimentally infected heterologous hosts. The complete genomes of SFV-1 (rhesus macaque virus), SFV-3 (African green monkey virus), and HFV have been cloned and sequenced. Depending on the region being compared, SFV-1 and SFV-3 show a closer sequence relationship (50% to 80%) to each other than either virus does with HFV (35% to 70%). Foamy viruses have also been isolated from healthy chimpanzees. To determine the precise genetic relationship of chimpanzee foamy virus with other retroviruses, we have cloned and sequenced the genomes of two independent virus isolates: (i) SFV-6 is a chimpanzee foamy virus obtained from the American Type Culture Collection and (ii) SFVcpz is an virus that we recovered from a B-cell line from a chimpanzee at the Southwest Foundation for Biomedical Research (San Antonio, TX). The cloned viruses encode gag, pol, and env genes as well as additional open reading frames (ORFs) in the 3' portion of the genome; one of these ORFs is a transcriptional transactivator (taf). SFVcpz, SFV-1, and SFV-3 each encode two ORFs (i.e., taf and ORF-1) whereas HFV encodes three ORFs (bel-1 or taf, bel2, and bel-3). Both chimpanzee viruses are greater than 98% homologous to each other; thus, independent viral isolates from the same species exhibit very little sequence variation. Predicted amino acid sequences of the gag, env, and taf genes of SIVcpz show 45% to 65% homology to counterpart proteins in either SFV-1 or SFV-3; the pol gene of SIVcpz has 78% to 80% homology at the amino acid level with the pol genes of either SFV-1 or SFV-3. Comparisons between SFVcpz and HFV reveal that the predicted gag, env, and taf protein sequences are 88% to 90% homologous, and the pol proteins are 95% related. In conclusion, the sequence information suggests that SFVcpz is an evolutionary link between foamy viruses of other non-human primates and HFV.
Publication Types:
Keywords:
- Animals
- Base Sequence
- Gene Products, pol
- Genes, env
- Genes, gag
- Genes, pol
- Humans
- In Vitro
- Open Reading Frames
- Pan troglodytes
- Primates
- Retroviridae
- Retroviridae Proteins
- Spumavirus
- Trans-Activators
- bel1 protein, Human foamy virus
- genetics
Other ID:
UI: 102207816
From Meeting Abstracts