RNase III

Patents

1.        "O²-aryl substituted diazeniumdiolates and use thereof," J. Saavedra, L. Keefer, A. Srinivasan, C. Bogdan, W. Rice, and X. Ji, Australia Patent Number 733590 (2001).

2.        "O²-arylated or O²-glycosylated 1-substituted diazen-1-ium-1,2-diolates and O²-substituted 1-[(2-carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolates," J.E. Saavedra, L.K. Keefer, A. Srinivasan, W.G. Rice, X. Ji, and C. Bogdan, US Patent Number 6610660 (2003).

3.        "O²-arylated or O²-glycosylated 1-substituted diazen-1-ium-1,2-diolates and O²-substituted 1-[(2-carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolates," J.E. Saavedra, L.K. Keefer, A. Srinivasan, C. Bogdan, W.G. Rice, and X. Ji, European Patent Number 0929538 (2004).

4.       “O²-arylated or O²-glycosylated 1-substituted diazen-1-ium-1,2-diolates and O²-substituted 1-[(2-carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolates,” J.E. Saavedra, L.K. Keefer, A. Srinivasan, W.G. Rice, and X. Ji, Germany Patent Number 69731758.7-0 (2005).

5.       “O²-substituted 1-[(2-carboxylato)pyrrolidin-1-yl]diazen-1-ium-1,2-diolates as nitric oxide (NO)-releasing prodrugs,” J.E. Saavedra, L.K. Keefer, A. Srinivasan, C. Bogdan, W.G. Rice, and X. Ji, US Divisional Patent Number 7081524 (2006).

Recent Publications

79.     “Crystal structure of the broadly cross-reactive HIV-1-neutralizing Fab X5 and fine mapping of its epitope,” R. Darbha, S. Phogat, A.F. Labrijn, Y. Shu, Y. Gu, M. Andrykovitch, M.-Y. Zhang, R. Pantophlet, L. Martin, C. Vita, D.R. Burton, D.S. Dimitrov, and X. Ji, Biochemistry (Accelerated Publications) 43, 1410-1417 (2004). [Abstract]

80.    “Essential roles of a dynamic loop in the catalysis of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase,” J. Blaszczyk, Y. Li, Y. Wu, G. Shi, X. Ji, and H. Yan, Biochemistry 43, 1469-1477 (2004). [Abstract]

81.     “Non-catalytic assembly of ribonuclease III with double-stranded RNA,” J. Blaszczyk, J. Gan, J.E. Tropea, D.L. Court, D.S. Waugh, and X. Ji, Structure 12, 457-466 (2004). [Abstract]

82.    “Reaction trajectory of pyrophosphoryl transfer catalyzed by a 6-hydroxymethyl-7,8- dihydropterin pyrophosphokinase,” J. Blaszczyk, G. Shi, Y. Li, H. Yan, and X. Ji, Structure 12, 467-475 (2004). [Abstract]

83.    "Tumor cell responses to a novel glutathione S-transferase-activated nitric oxide releasing prodrug," V.J. Findlay, D.M. Townsend, J.E. Saavedra, G.S. Buzard, M.L. Citro, L.K. Keefer, X. Ji, and K.D. Tew, Mol. Pharmacol. 65, 1070-1079 (2004).[Abstract]

84.    "Impact of antibody framework residue V_H-71 on the stability of a humanised anti-MUC1 scFv and derived immunoenzyme," J. Krauss, M.A.E. Arndt, Z. Zhu, D. L. Newton, B.K. Vu, V. Choudhry, R. Darbha, X. Ji, N.S. Courtenay-Luck, M.P. Deonarain, J. Richards, and S.M. Rybak, Br. J. Cancer, 90, 1863-1870 (2004). [Abstract]

85.    "Structural Basis for Catalytic Differences between Alpha Class Human Glutathione Transferases hGSTA1-1 and hGSTA2-2 for Glutathione Conjugation of Environmental Carcinogen Benzo[a]pyrene-7,8-diol-9,10-epoxide," S. V. Singh, V. Varma, P. Zimniak, S. K. Srivastava, S.W. Marynowski, S. Amin, and X. Ji, Biochemistry, 43, 9708-9715 (2004). [Abstract]

86.    "Crystal structure of human glutathione S-transferase A3-3 and mechanistic implications for its high steroid isomerase activity," Y. Gu, A. Pal, S.-S. Pan, S.V. Singh, and X. Ji, Biochemistry 43, 15673-15679 (2004). [Abstract]

87.    "Structural basis for the function of stringent starvation protein A as a transcription factor," A.-M. Hansen, Y. Gu, M. Li, M. Andrykovitch, D.S. Waugh, D.J. Jin, and X. Ji, J. Biol. Chem. 208, 17380-17391 (2005). [Abstract]

88.    "Is the Critical Role of Loop 3 of Escherichia coli 6-Hydroxymethyl-7,8-dihydropterin Pyrophosphokinase in Catalysis Due to Loop-3 Residues Arginine-84 and Tryptophan-89? Site-directed Mutagenesis, Biochemical, and Crystallographic Studies," Y. Li, J. Blaszczyk, Y. Wu, G. Shi, X. Ji, and H. Yan, Biochemistry 44, 8590-8599 (2005). [Abstract]

89.    "Intermediate states of ribonuclease III in complex with double-stransed RNA," J. Gan, J.E. Tropea, B.P. Austin, D.L. Court, D.S. Waugh, and X. Ji, Structure 13, 1435-1442 (2005). [Abstract]

90.    "A glutathione S-transferase π activated pro-drug causes kinase activation concurrent with S-glutathionylation of proteins,” D.M. Townsend, V.J. Findlay, F. Fazilev, M. Ogle, J. Fraser, J. Saavedra, X. Ji, L. Keefer, and K.D. Tew, Molecular Pharmacology 69, 501-508 (2006, online 11/2005). [Abstract]

91.    “Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III,” J. Gan, J.E. Tropea, B.P. Austin, D.L. Court, D.S. Waugh, and X. Ji, Cell 124, 355-366 (2006). [Abstract]

92.    “PABA/NO as an anticancer lead: Analogue synthesis, structural revision, solution chemistry, reactivity toward glutathione, and in vitro activity,” J.E. Saavedra, A. Srinivasan, G.S. Buzard, K.M. Davies, D.J. Waterhouse, K. Inami, T.C. Wilde, M.L. Citro, M. Cuellar, J.R. Deschamps, D. Parrish, P.J. Sharmi, V.J. Findlay, D.M. Townsend, K.D. Tew, S.V. Singh, L. Jia, X. Ji, and L.K. Keefer, J. Med. Chem. (online 01/2006). [Abstract]

93.    “Structural mimicry of CD4 by a cross-reactive HIV-1 neutralizing antibody with CDR-H2 and H3 containing unique motifs,” P.Prabakaran, J. Gan, Y.-Q. Wu, M.-Y. Zhang, D.S. Dimitrov, and X. Ji, J. Mol. Biol. (online 01/2006). [Abstract]

94.    “Structure of severe acute respiratory syndrome coronavirus receptor-binding domain complexed with neutralizing antibody,” P. Prabakaran, J. Gan, Y. Feng, Z. Zhu, V. Choudhry, X. Xiao, X. Ji, and D.S. Dimitrov, J. Biol. Chem. 281, 15829-15836 (2006). [Abstract]

95.    “Crystal structure of Mycobacterium tuberculosis shikimate kinase in complex with shikimic acid and an ATP analog,” J. Gan, Y. Gu, Y. Li, H. Yan, and X. Ji, Biochemistry 45, 8539-8545 (2006). [Abstract]

96.    “Antitumor activity of JS-K [O2-(2,4-dinitrophenyl) 1-[(4-ethoxycarbonyl)piperazin-1-yl]diazen-1-ium-1,2-diolate] and related O2-aryl diazeniumdiolates in vitro and in vivo,” P.J. Shami, J.E. Saavedra, C.L. Bonifant, J. Chu, B.I. Carr, S. Kar, M. Wang, L. Jia, X. Ji,  L.K. Keefer, J. Med. Chem. 49, 4356-4366 (2006). [Abstract]

97.    “Structural basis for non-catalytic and catalytic activities of ribonuclease III,” X. Ji, Acta Crystallogr. D62, 933-940 (2006). [Abstract]

98.    “Structural basis for the aldolase and epimerase activities of Staphylococcus aureus dihydroneopterin aldolase,” J. Blaszczyk, Y. Li, J. Gan, H. Yan and X. Ji, J. Mol. Biol. 368, 161-169 (2007). [Abstract]

99.    “Structural and Biochemical Analyses of Shikimate Dehydrogenase AroE from Aquifex aeolicus: Implications for the Catalytic Mechanism,” J. Gan, Y. Wu, P. Prabakaran, Y. Gu, Y. Li, M. Andrykovitch, H. Liu, Y. Gong, H. Yan, and X. Ji, Biochemistry 46, 9513-9522 (2007). [Abstract]

100.  “Structure and activity of Yersinia pestis6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase as a novel target for the development of antiplague therapeutics,” J. Blaszczyk, Y. Li, S. Cherry, J. Alexandratos, Y. Wu, G. Shaw, J.E. Tropea, D.S. Waugh, H. Yan, and X. Ji, Acta Crystallogr. D63, 1169-1177 (2007). [Abstract]

101.  “A stepwise model for double-stranded RNA processing by ribonuclease III,” J. Gan, G. Shaw, J.E. Tropea, D.S. Waugh, D.L. Court and X. Ji, Mol. Microbiol. 67, 143-154 (2008). [Abstract]

102.  “The mechanism of RNase III action: How Dicer dices,” X. Ji, in Current Topics in Microbiology and Immunology, Volume 320, RNAi, P.J. Paddison and P.K. Vogt (eds), Springer Publishers, New York, pp 99-116 (2008). [Abstract] 

103.  “Impact of low-frequency hotspot mutation R282Q on the structure of p53 DNA-binding domain as revealed by crystallography at 1.54-Å resolution,” C. Tu, Y.-H. Tan, G. Shaw, Z. Zhou, Y. Bai, R. Luo, and X. Ji, Acta Crystallogr. D64, 471-477 (2008). [Abstract]

104.  “Structure-based design of anticancer prodrug PABA/NO,” X. Ji, A. Pal, R. Kalathur, X. Hu, Y. Gu, J.E. Saavedra, G.S. Buzard, A. Srinivasan, L. Keefer, and S.V. Singh, Drug Design, Development and Therapy 2, 123-130 (2008). [Abstract]

105.  “Crystal structure of an isolated unglycosylated antibody CH2 domain,” P. Prabakaran, B.K. Vu, J. Gan, Y. Feng, D.S. Dimitrov, and X. Ji, Acta Crystallogr. D64, 1062-1067 (2008). [Abstract]

106.  “Structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription,” G. Shaw, Y.N. Zhou, H. Zhi, P. Subburaman, R. Zhang, A. Joachimiak, D.J. Jin, and X. Ji, Structure 16, 1417-1427 (2008). [Abstract]