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Library:4383 (dbEST ID)
PT0052
Library Description
| Organism: | Homo sapiens |
| Developmental stage: | Adult |
| Organ: | pnet |
| Vector: | puc18 |
| Vector type: | Plasmid |
| Cloning site 1: | SmaI |
| Cloning site 2: | SmaI |
A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.
Source: Simpson A.J.G., Ludwig Institute for Cancer Research
Gene Content Analysis
76 ESTs from this library were grouped into 36 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 17 | 223684 |  |
Hs.517145 | Enolase 1, (alpha). |
| 7 | 92105 | |
Hs.584864 | Prostaglandin reductase 1. |
| 6 | 78947 | |
Hs.500375 | Ectonucleoside triphosphate diphosphohydrolase 6 (putative function). |
| 5 | 65789 | |
Hs.643452 | DiGeorge syndrome critical region gene 8. |
| 3 | 39474 | |
Hs.596704 | Staufen, RNA binding protein, homolog 1 (Drosophila). |
| 3 | 39474 | |
Hs.435736 | CDNA FLJ46553 fis, clone THYMU3038879. |
| 2 | 26316 | |
Hs.8008 | DNA methyltransferase 1 associated protein 1. |
| 2 | 26316 | |
Hs.533282 | Non-POU domain containing, octamer-binding. |
| 2 | 26316 | |
Hs.495349 | HLA-B associated transcript 2-like. |
| 2 | 26316 | |
Hs.472330 | Chromosome 20 open reading frame 3. |
| 2 | 26316 | |
Hs.40888 | Activity-regulated cytoskeleton-associated protein. |
| 1 | 13158 | |
Hs.80976 | Antigen identified by monoclonal antibody Ki-67. |
| 1 | 13158 | |
Hs.706999 | Transcribed locus, moderately similar to NP_065392.1 chromosome 20 open reading frame 3 [Homo sapiens]. |
| 1 | 13158 | |
Hs.69851 | GAR1 ribonucleoprotein homolog (yeast). |
| 1 | 13158 | |
Hs.665555 | Transcribed locus. |
| 1 | 13158 | |
Hs.658000 | Myosin ID. |
| 1 | 13158 | |
Hs.654794 | Neuralized homolog 4 (Drosophila). |
| 1 | 13158 | |
Hs.644906 | Heterogeneous nuclear ribonucleoprotein L. |
| 1 | 13158 | |
Hs.643464 | DIS3 mitotic control homolog (S. cerevisiae). |
| 1 | 13158 | |
Hs.63788 | Propionyl Coenzyme A carboxylase, beta polypeptide. |
| 1 | 13158 | |
Hs.563978 | Transcribed locus. |
| 1 | 13158 | |
Hs.517044 | PHD finger protein 20. |
| 1 | 13158 | |
Hs.509622 | Ral guanine nucleotide dissociation stimulator-like 2. |
| 1 | 13158 | |
Hs.506374 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12. |
| 1 | 13158 | |
Hs.484068 | RAB40B, member RAS oncogene family. |
| 1 | 13158 | |
Hs.483305 | Histidine triad nucleotide binding protein 1. |
| 1 | 13158 | |
Hs.472831 | Protein kinase (cAMP-dependent, catalytic) inhibitor gamma. |
| 1 | 13158 | |
Hs.471637 | DIS3 mitotic control homolog (S. cerevisiae)-like 2. |
| 1 | 13158 | |
Hs.462915 | G protein-coupled receptor 179. |
| 1 | 13158 | |
Hs.445775 | Protein tyrosine phosphatase, non-receptor type 9. |
| 1 | 13158 | |
Hs.436667 | Pregnancy up-regulated non-ubiquitously expressed CaM kinase. |
| 1 | 13158 | |
Hs.408153 | Proline rich 4 (lacrimal). |
| 1 | 13158 | |
Hs.302903 | Ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast). |
| 1 | 13158 | |
Hs.247280 | RanBP-type and C3HC4-type zinc finger containing 1. |
| 1 | 13158 | |
Hs.20274 | Solute carrier family 41, member 1. |
| 1 | 13158 | |
Hs.130607 | Mevalonate kinase. |
Source: NCBI UniGene
EST Sequences
216 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- uncharacterized tissue
- peripheral neuroectodermal tumor
- adult
Source: NCBI Clone Registry
References
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).