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Experiment Id: 11569
Pattern Id: MT1277
Clone number:
GenBank Acc:
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Rationale: The solid tumor lines were characterized individually using immunocytochemical techniques to determine their relative degrees of representativity for the subpanels within which they are grouped.
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Investigator(s):
Stinson, Dr. Sherman
NCI-FCRDC
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Method:
Immunostaining
Unit:
Percent positive cells
Protocol:
Cells were grown to near-confluence and harvested with a cell scaper. Cytology specimens were propared on glass or Teflon-coated slides. Immunostaining was performed on air-dired, unfixed specimens (for cell surface antigens) or on actone fixed specimens (for intracellular antigens) with commercially available kits. Slides were prepared froma minimum of 2 separate cultures for each cell line. Micorscopically, 3 separate fields, each with ~100 cells, were selected from each slide and the % positive stained cells was counted. Results from all fields and slides were averaged and rounded to the nearest 5%.
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Target Data:
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Compare:
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