MAJOR MYCOTOXINS Aflatoxins The aflatoxins were isolated and characterized after the death of more than 100,000 turkey poults (turkey X disease) was traced to the consumption of a mold-contaminated peanut meal ( 22, 91). The four major aflatoxins are called B 1, B 2, G 1, and G 2 based on their fluorescence under UV light (blue or green) and relative chromatographic mobility during thin-layer chromatography. Aflatoxin B 1 (Fig. 1) is the most potent natural carcinogen known ( 243) and is usually the major aflatoxin produced by toxigenic strains. It is also the best studied: in a large percentage of the papers published, the term aflatoxin can be construed to mean aflatoxin B 1. However, well over a dozen other aflatoxins (e.g., P 1. Q 1, B 2a, and G 2a) have been described, especially as mammalian biotransformation products of the major metabolites ( 104). The classic book Aflatoxin: Scientific Background, Control, and Implications, published in 1969 ( 91), is still a valuable resource for reviewing the history, chemistry, toxicology, and agricultural implications of aflatoxin research. Aflatoxins are difuranocoumarin derivatives produced by a polyketide pathway by many strains of Aspergillus flavus and Aspergillus parasiticus; in particular, Aspergillus flavus is a common contaminant in agriculture. Aspergillus bombycis, Aspergillus ochraceoroseus, Aspergillus nomius, and Aspergillus pseudotamari are also aflatoxin-producing species, but they are encountered less frequently ( 92, 139, 197). From the mycological perspective, there are great qualitative and quantitative differences in the toxigenic abilities displayed by different strains within each aflatoxigenic species. For example, only about half of Aspergillus flavus strains produce aflatoxins ( 138), while those that do may produce more than 10 6 μg/kg ( 51). Many substrates support growth and aflatoxin production by aflatoxigenic molds. Natural contamination of cereals, figs, oilseeds, nuts, tobacco, and a long list of other commodities is a common occurrence ( 63, 65). Like the genetic ability to make aflatoxin, contamination is highly variable. Sometimes crops become contaminated with aflatoxin in the field before harvest, where it is usually associated with drought stress ( 65, 137); even more problematic is the fate of crops stored under conditions that favor mold growth. In storage, usually the most important variables are the moisture content of the substrate and the relative humidity of the surroundings ( 63, 280). Aflatoxin contamination has been linked to increased mortality in farm animals and thus significantly lowers the value of grains as an animal feed and as an export commodity ( 238). Milk products can also serve as an indirect source of aflatoxin. When cows consume aflatoxin-contaminated feeds, they metabolically biotransform aflatoxin B 1 into a hydroxylated form called aflatoxin M 1 ( 267). Aflatoxin is associated with both toxicity and carcinogenicity in human and animal populations ( 70, 186, 193, 232). The diseases caused by aflatoxin consumption are loosely called aflatoxicoses. Acute aflatoxicosis results in death; chronic aflatoxicosis results in cancer, immune suppression, and other “slow” pathological conditions ( 121). The liver is the primary target organ, with liver damage occurring when poultry, fish, rodents, and nonhuman primates are fed aflatoxin B 1. There are substantial differences in species susceptibility. Moreover, within a given species, the magnitude of the response is influenced by age, sex, weight, diet, exposure to infectious agents, and the presence of other mycotoxins and pharmacologically active substances. Thousands of studies on aflatoxin toxicity have been conducted, mostly concerning laboratory models or agriculturally important species ( 56, 70, 186). Cytochrome P450 enzymes convert aflatoxins to the reactive 8,9-epoxide form (also referred to as aflatoxin-2,3 epoxide in the older literature), which is capable of binding to both DNA and proteins ( 70). Mechanistically, it is known that the reactive aflatoxin epoxide binds to the N 7 position of guanines. Moreover, aflatoxin B 1-DNA adducts can result in GC to TA transversions. A reactive glutathione S-transferase system found in the cytosol and microsomes catalyzes the conjugation of activated aflatoxins with reduced glutathione, leading to the excretion of aflatoxin ( 208). Variation in the level of the glutathione transferase system as well as variations in the cytochrome P450 system are thought to contribute to the differences observed in interspecies aflatoxin susceptibility ( 70, 71). Because of the differences in aflatoxin susceptibility in test animals, it has been difficult to extrapolate the possible effects of aflatoxin to humans, but acute toxicity of aflatoxins in Homo sapiens has not been observed very often. It is believed that a 1974 Indian outbreak of hepatitis in which 100 people died may have been due to the consumption of maize that was heavily contaminated with aflatoxin. Some adults may have eaten 2 to 6 mg of aflatoxin in a single day ( 141). Subsequently, it was calculated that the acute lethal dose for adults is approximately 10 to 20 mg of aflatoxins ( 200). One anecdotal report refutes this estimate. A woman who had ingested over 40 mg of purified aflatoxin in a suicide attempt was still alive 14 years later. Multiple laboratory tests of her urine and blood, and X-ray, ultrasound, and computerized axial tomography analyses of her abdomen, liver, and spleen all gave normal results ( 279). It has been hypothesized that kwashiorkor, a severe malnutrition disease, may be a form of pediatric aflatoxicosis ( 109). Further early speculations that aflatoxin might be involved in Reye's syndrome, an encephalopathy, and fatty degeneration of the viscera in children and adolescents ( 102) have not been substantiated. Nevertheless, aflatoxin has achieved some notoriety as a poison. The plot of The Human Factor, a spy thriller by Graham Greene ( 95), revolves around the murder of a central figure whose whiskey was laced with aflatoxin (a toxicologically improbable way to kill someone). Nevertheless, aflatoxin's reputation has a potent poison may explain why it has been adopted for use in bioterrorism. There is substantial evidence that Iraq stockpiled aflatoxin to be delivered in missiles (see section on bioterrorism below). The data on aflatoxin as a human carcinogen are far more damning than the data implicating it in acute human toxicities. Exposure to aflatoxins in the diet is considered an important risk factor for the development of primary hepatocellular carcinoma, particularly in individuals already exposed to hepatitis B. In classical epidemiology, several studies have linked liver cancer incidence to estimated aflatoxin consumption in the diet ( 152, 193, 270). The results of these studies have not been entirely consistent, and quantification of lifetime individual exposure to aflatoxin is extremely difficult. The incidence of liver cancer varies widely from country to country, but it is one of the most common cancers in China, the Philippines, Thailand, and many African countries. The presence of hepatitis B virus infection, an important risk factor for primary liver cancer, complicates many of the epidemiological studies. In one case-control study involving more than 18,000 urine samples collected over 3.5 years in Shanghai, China, aflatoxin exposure alone yielded a relative risk of about 2; hepatitis B virus antigen alone yielded a relative risk of about 5; combined exposure to aflatoxin and hepatitis B yielded a relative risk of about 60 ( 217). Vaccination against hepatitis B virus is recommended as a more realistic and cost-effective strategy for lowering liver cancer incidence than removing aflatoxin from the diet ( 111, 112). In molecular epidemiology, it is possible to demonstrate with more certainty the association between putative carcinogens and specific cancers. Biomonitoring of aflatoxins can be done by analyzing for the presence of aflatoxin metabolites in blood, milk, and urine; moreover, excreted DNA adducts and blood protein adducts can also be monitored ( 221). The aflatoxin B 1-N 7-guanine adduct represents the most reliable urinary biomarker for aflatoxin exposure but reflects only recent exposure. Numerous studies have shown that carcinogenic potency is highly correlated with the extent of total DNA adducts formed in vivo ( 69, 70). Inactivation of the p53 tumor suppressor gene may be important in the development of primary hepatocellular carcinoma. Studies of liver cancer patients in Africa and China have shown that a mutation in the p53 tumor suppressor gene at codon 249 is associated with a G-to-T transversion ( 23, 122). Mechanistically, it is known that the reactive aflatoxin epoxide binds to the N 7 position of guanines. Moreover, aflatoxin B 1-DNA adducts can result in GC to TA transversions. The specific mutation in codon 249 of the p53 gene has been called the first example of a “carcinogen-specific” biomarker that remains fixed in the tumor tissue ( 69). There is also considerable evidence associating aflatoxin with neoplasms in extrahepatic tissues, particularly the lungs. For example, one early epidemiological study of Dutch peanut processing workers exposed to dust contaminated with aflatoxin B 1 showed a correlation between both respiratory cancer and total cancer in the exposed group compared with unexposed cohorts ( 103). Anecdotal and circumstantial evidence of air-borne aflatoxin exposure leading to cancer has been reported. Deger ( 59) concluded that dust from scrapings of chromatographic plates from aflatoxin analyses contributed to causing cancer in two young adults. Aflatoxin is a pulmonary carcinogen in experimental animals. The nonhepatic effects of aflatoxin B 1 have been summarized by Coulombe ( 52). To recapitulate, there is no other natural product for which the data on human carcinogenicity are so compelling. The International Agency for Research on Cancer has classified aflatoxin B 1 as a group I carcinogen ( 127). In developed countries, sufficient amounts of food combined with regulations that monitor aflatoxin levels in these foods protect human populations from significant aflatoxin ingestion. However, in countries where populations are facing starvation or where regulations are either not enforced or nonexistent, routine ingestion of aflatoxin may occur ( 51). Worldwide, liver cancer incidence rates are 2 to 10 times higher in developing countries than in developed countries ( 112). Unfortunately, strict limitation of aflatoxin-contaminated food is not always an option. A joint Food and Agriculture Organization/World Health Organization/United Nations Environment Programme Conference report stated that “in developing countries, where food supplies are already limited, drastic legal measure may lead to lack of food and to excessive prices. It must be remembered that people living in these countries cannot exercise the option of starving to death today in order to live a better life tomorrow” (quoted in Henry et al. [ 112]). There has been considerable fundamental work on the aflatoxins, especially on their biosynthesis and molecular biology. The first stable step in the biosynthetic pathway is the production of norsolorinic acid, an anthraquinone precursor, by a type II polyketide synthase. An elaborate series of at least 15 post-polyketide synthase steps follows, yielding a series of increasingly toxigenic metabolites ( 12, 48, 114, 192, 256, 257). Sterigmatocystin, a related dihydrofuran toxin, is a late metabolite in the aflatoxin pathway and is also produced as a final biosynthetic product by a number of species such as Aspergillus versicolor and Aspergillus nidulans. Sterigmatocystin is both mutagenic and tumorigenic but is less potent than aflatoxin ( 14). Analysis of the molecular genetics of sterigmatocystin biosynthesis in the genetically tractable species Aspergillus nidulans has provided a useful model system. The genes for the sterigmatocystin gene cluster from Aspergillus nidulans have been cloned and sequenced ( 25). Cognate genes for aflatoxin pathway enzymes from Aspergillus flavus and Aspergillus parasiticus show high sequence similarity to the sterigmatocystin pathway genes ( 192, 288, 289). The organization of genes in the Aspergillus flavus, Aspergillus nidulans, and Aspergillus parasiticus sterigmatocystin-aflatoxin pathway has been compared by Cary et al. ( 34) and Hicks et al. ( 114). Finally, it should be mentioned that Aspergillus oryzae and Aspergillus sojae, species that are widely used in Asian food fermentations such as soy sauce, miso, and sake, are closely related to the aflatoxigenic species Aspergillus flavus and Aspergillus parasiticus. Although these food fungi have never been shown to produce aflatoxin ( 276), they contain homologues of several aflatoxin biosynthesis pathway genes ( 140). Deletions and other genetic defects have led to silencing of the aflatoxin pathway in both Aspergillus oryzae and Aspergillus sojae ( 254, 274). Citrinin Citrinin (Fig. 2) was first isolated from Penicillium citrinum prior to World War II ( 113); subsequently, it was identified in over a dozen species of Penicillium and several species of Aspergillus (e.g., Aspergillus terreus and Aspergillus niveus), including certain strains of Penicillium camemberti (used to produce cheese) and Aspergillus oryzae (used to produce sake, miso, and soy sauce) ( 158). More recently, citrinin has also been isolated from Monascus ruber and Monascus purpureus, industrial species used to produce red pigments ( 21). Citrinin has been associated with yellow rice disease in Japan ( 222). It has also been implicated as a contributor to porcine nephropathy. Citrinin acts as a nephrotoxin in all animal species tested, but its acute toxicity varies in different species ( 33). The 50% lethal dose for ducks is 57 mg/kg; for chickens it is 95 mg/kg; and for rabbits it is 134 mg/kg ( 100). Citrinin can act synergistically with ochratoxin A to depress RNA synthesis in murine kidneys ( 223). For a review of the early literature, see Krogh ( 142). Wheat, oats, rye, corn, barley, and rice have all been reported to contain citrinin ( 2). With immunoassays, citrinin was detected in certain vegetarian foods colored with Monascus pigments ( 39). Citrinin has also been found in naturally fermented sausages from Italy ( 4). Although citrinin is regularly associated with human foods, its significance for human health is unknown. Ergot Alkaloids The ergot alkaloids are among the most fascinating of fungal metabolites. They are classified as indole alkaloids and are derived from a tetracyclic ergoline ring system. Lysergic acid, a structure common to all ergot alkaloids, was first isolated in 1934. The clavines have ergoline as a basic structure but lack peptide components; the lysergic acid alkaloids include ergotamine and lysergic acid amide (ergine) ( 11). The structure of ergotamine is shown in Fig. 3. These compounds are produced as a toxic cocktail of alkaloids in the sclerotia of species of Claviceps, which are common pathogens of various grass species. The ingestion of these sclerotia, or ergots, has been associated with diseases since antiquity. An Assyrian tablet dated to 600 B.C.E., referring to a “noxious pustule in the ear of grain,” is believed to be an early reference to ergot ( 120). The human disease acquired by eating cereals infected with ergot sclerotia, usually in the form of bread made from contaminated flour, is called ergotism or St. Anthony's fire. Two forms of ergotism are usually recognized, gangrenous and convulsive. The gangrenous form affects the blood supply to the extremities, while convulsive ergotism affects the central nervous system ( 11). Human ergotism was common in Europe in the Middle Ages. For example, a three-volume work entitled Handbook of Geographical and Historical Pathology published in London by August Hirch between 1883 and 1886 recorded 132 epidemics of European ergotism between the 6th and 18th centuries ( 98). Matossian ( 170) has suggested that the “slow nervous fever” described by the 18th century English physician Jon Huxham may be another example of human ergotism. Slow nervous fever usually occurred in the summer and fall after a severe winter; Huxham suspected “bad food” as the source of the trouble. Matossian ( 171) has also postulated that ergot alkaloids may have had a strong influence on fertility trends in England and other European countries during the 17th and 18th centuries. Modern methods of grain cleaning have almost eliminated ergotism as a human disease. Nevertheless, purported ergot poisoning occurred in the French town of Pont-St.-Esprit in 1951 and was the subject of a full-length book treatment, The Day of St. Anthony's Fire ( 85). Ergotism is still an important veterinarian problem. The principal animals at risk are cattle, sheep, pigs, and chickens. Clinical symptoms of ergotism in animals include gangrene, abortion, convulsions, suppression of lactation, hypersensitivity, and ataxia ( 154). Sometimes the line between toxin and drug is defined with the shift of a decimal point or a change in a small chemical moiety. The ergot alkaloids are a case in point. Their myriad actions have long engaged the interest of physicians and pharmacologists. Several ergot alkaloids induce smooth muscle contractions. For centuries it had been observed that grazing on grass infected with ergot caused abortion in pregnant farm animals, so it is not surprising that midwives and others adopted ergot as a folk medicine, using it as both an abortifacient and a drug to accelerate to uterine contractions for women in labor ( 213). During the 20th century, the famous hallucinogen lysergic acid diethylamide (LSD) was discovered as the result of research with ergot alkaloids conducted at the Sandoz Laboratories in Basel, Switzerland. A chemist named Hofmann combined different amines in peptide linkage with lysergic acid to produce ergobasine (also called ergometrine and ergonovine), the first semisynthetic ergot alkaloid. Then, by varying the amino alcohol constituent, he obtained Methergine, a compound prescribed widely for decades to control hemorrhage after childbirth. Hofmann continued to synthesize new lysergic acid derivatives; the 25th substance in his series was d-lysergic acid diethylamide (LSD-25). In 1943, after accidentally ingesting some of the compound, he discovered the hallucinogenic properties of this semisynthetic derivative ( 120). For a while, Sandoz marketed LSD to psychiatrists under the trademark Delysid. It was used unsuccessfully to treat schizophrenia. In a bizarre chapter of American history, the Central Intelligence Agency, under the code name MK- ULTRA, used LSD as a truth serum for interrogating suspected communists ( 262). More recently, pure ergotamine has been used for the treatment of migraine headaches. Other ergot derivatives are used as prolactin inhibitors, in the treatment of Parkinsonism, and in cases of cerebrovascular insufficiency ( 11). The therapeutic administration of ergot alkaloids may cause sporadic cases of human ergotism ( 30). Finally, it has been hypothesized that the Salem witchcraft affair may have been a form of convulsive ergotism related to consumption of rye infected with Claviceps sclerotia ( 32, 169). Although some historians dispute this hypothesis ( 242), the epidemiological and clinical data are quite provocative ( 11). Robin Cook, the author of a number of bestsellers, used the Salem-ergot hypothesis as the basis for a novel in which a young physician isolates Claviceps spores from a damp New England cellar, cultures the fungus, and then discovers a new alkaloid with mind- and energy-enhancing properties. Taking a cue from the Central Intelligence Agency, he names his drug Ultra and founds a biotechnology company in order to reap profits from the would-be “billion-dollar molecule.” In the end it is discovered that the euphoria-inducing fictional alkaloid has horrifying side effects ( 50). Cook's novel is our personal favorite example of mycotoxins in popular culture. Fumonisins Fumonisins were first described and characterized in 1988 ( 17, 87). The most abundantly produced member of the family is fumonisin B 1 (Fig. 4). They are thought to be synthesized by condensation of the amino acid alanine into an acetate-derived precursor ( 250). Fumonisins are produced by a number of Fusarium species, notably Fusarium verticillioides (formerly Fusarium moniliforme = Gibberella fujikuroi), Fusarium proliferatum, and Fusarium nygamai, as well as Alternaria alternata f. sp. lycopersici ( 164, 210). These fungi are taxonomically challenging, with a complex and rapidly changing nomenclature which has perplexed many nonmycologists (and some mycologists, too) ( 151, 165). The major species of economic importance is Fusarium verticillioides, which grows as a corn endophyte in both vegetative and reproductive tissues, often without causing disease symptoms in the plant. However, when weather conditions, insect damage, and the appropriate fungal and plant genotype are present, it can cause seedling blight, stalk rot, and ear rot ( 185). Fusarium verticillioides is present in virtually all corn samples ( 167, 164). Most strains do not produce the toxin, so the presence of the fungus does not necessarily mean that fumonisin is also present ( 203). Although it is phytotoxic, fumonisin B 1 is not required for plant pathogenesis ( 60, 160). Fumonisins affect animals in different ways by interfering with sphingolipid metabolism ( 68, 161, 175, 273). They cause leukoencephalomalacia (hole in the head syndrome) in equines ( 163) and rabbits ( 27); pulmonary edema and hydrothorax in swine ( 101); and hepatotoxic and carcinogenic effects ( 87, 88, 89) and apoptosis in the liver of rats ( 204). In humans, there is a probable link with esophageal cancer ( 252). The occurrence of fumonisin B 1 is correlated with the occurrence of a higher incidence of esophageal cancer in regions of Transkei (South Africa), China, and northeast Italy ( 195). It has been isolated at high levels in corn meal and corn grits, including seven samples from a supermarket in Charleston, S.C., a city which has the highest incidence of esophageal cancer among African-Americans in the United States ( 252). Several other mycotoxins, nutritional parameters, and other factors have also been implicated in the etiology of human esophageal cancer; see Beardall and Miller ( 7) for an excellent discussion of the way in which multiple etiological factors are suspected to interact. A possible case of acute exposure to fumonisin B 1 involved 27 villages in India, where consumption of unleavened bread made from moldy sorghum or corn caused transient abdominal pain, borborygmus, and diarrhea. All those affected recovered fully ( 18). Finally, fumonisins can cause neural tube defects in experimental animals and thus may also have a role in human cases. It has been hypothesized that a cluster of anencephaly and spina bifida cases in southern Texas may have been related to fumonisins in corn products ( 107, 108, 181). The International Agency for Research on Cancer has evaluated the cancer risk of fumonisins to humans and classified them as group 2B (probably carcinogenic) ( 210). Unlike most known mycotoxins, which are soluble in organic solvents, fumonisins are hydrophilic. This makes them difficult to study. Usually they are extracted in aqueous methanol or aqueous acetonitrile ( 20). High-performance liquid chromatography with fluorescent detection is the most widely used analytic method ( 202). The fumonisin story raises the specter that there may be many other occult but toxic products of fungal metabolism that have not yet been discovered because of their hydrophilic nature. Ochratoxin Ochratoxin A (Fig. 5) was discovered as a metabolite of Aspergillus ochraceus in 1965 during a large screen of fungal metabolites that was designed specifically to identify new mycotoxins ( 266). Shortly thereafter, it was isolated from a commercial corn sample in the United States ( 234) and recognized as a potent nephrotoxin. Members of the ochratoxin family have been found as metabolites of many different species of Aspergillus, including Aspergillus alliaceus, Aspergillus auricomus, Aspergillus carbonarius, Aspergillus glaucus, Aspergillus melleus, and Aspergillus niger ( 1, 6, 46). Because Aspergillus niger is used widely in the production of enzymes and citric acid for human consumption, it is important to ensure that industrial strains are nonproducers ( 105, 255). Although some early reports implicated several Penicillium species, it is now thought that Penicillium verrucosum, a common contaminant of barley, is the only confirmed ochratoxin producer in this genus ( 41, 199). Nevertheless, many mycotoxin reviews reiterate erroneous species lists. As with other mycotoxins, the substrate on which the molds grow as well as the moisture level, temperature, and presence of competitive microflora interact to influence the level of toxin produced. Ochratoxin A has been found in barley, oats, rye, wheat, coffee beans, and other plant products, with barley having a particularly high likelihood of contamination. There is also concern that ochratoxin may be present in certain wines, especially those from grapes contaminated with Aspergillus carbonarius ( 166, 200, 269). Of the Aspergillus toxins, only ochratoxin is potentially as important as the aflatoxins. The kidney is the primary target organ. Ochratoxin A is a nephrotoxin to all animal species studied to date and is most likely toxic to humans, who have the longest half-life for its elimination of any of the species examined ( 54). In addition to being a nephrotoxin, animal studies indicate that ochratoxin A is a liver toxin, an immune suppressant, a potent teratogen, and a carcinogen ( 7, 146). Ochratoxin A disturbs cellular physiology in multiple ways, but it seems that the primary effects are associated with the enzymes involved in phenylalanine metabolism, mostly by inhibiting the enzyme involved in the synthesis of the phenylalanine-tRNA complex ( 28, 166). In addition, it inhibits mitochondrial ATP production ( 174) and stimulates lipid peroxidation ( 207). Ochratoxin has been detected in blood and other animal tissues and in milk, including human milk ( 166). It is frequently found in pork intended for human consumption ( 80). Ochratoxin is believed to be responsible for a porcine nephropathy that has been studied intensively in the Scandinavian countries. The disease is endemic in Denmark, where rates of porcine nephropathy and ochratoxin contamination in pig feed are highly correlated ( 143). In addition, ochratoxin is associated with disease and death in poultry ( 29, 99). There has been speculation that ochratoxins are involved in a human disease called endemic Balkan nephropathy ( 123, 143). This condition is a progressive chronic nephritis that occurs in populations who live in areas bordering the Danube River in parts of Romania, Bulgaria, and the former Yugoslavia. In one Bulgarian study, ochratoxin contamination of food and the presence of ochratoxin in human serum were more common in families with endemic Balkan nephropathy and urinary tract tumors than in unaffected families ( 35). In addition to ochratoxin poisoning, this curious disease has been attributed to genetic factors, heavy metals, and possible occult infectious agents. The current consensus is that endemic Balkan nephropathy is of unknown etiology, but many mycotoxin reviews list it, without caveat, as an ochratoxicosis. It has also been hypothesized that the gene for phenylketonuria might occur in relatively high frequency because of a heterozygous advantage against ochratoxin poisoning ( 284) and that ochratoxin might be a risk factor for testicular cancer ( 226). How common is human exposure to ochratoxin? Studies from Canada, Sweden, West Germany, and Yugoslavia detected ochratoxin in human blood and serum ( 146). Analyses of urine from children in Sierra Leone detected both ochratoxin and aflatoxin throughout the year ( 135). Several detailed risk assessments have been conducted for ochratoxin A ( 146). Given the known human exposure and the abundance of toxicological data from animal studies, the European Union Scientific Committee has recommended that ochratoxin A levels be reduced to below 5 ng/kg of body weight per day ( 251). In addition, several European countries have proposed individual regulations, with maximum tolerated concentrations varying greatly from country to country ( 131, 268). The International Agency for Research on Cancer has rated ochratoxin as a possible human carcinogen (category 2B) ( 7). In conclusion, it would be prudent for the medical community to pay closer attention to the possibility of ochratoxin toxicity in patients with symptoms of renal pathology ( 54). Although the role of ochratoxin A in human disease is still speculative, its acute nephrotoxicity, immunosuppressive actions, and teratogenic effects in animal models, coupled with its ability to be carried through the food chain, merit concern. Patulin Patulin, 4-hydroxy-4H-furo[3,2c]pyran-2(6H)-one, is produced by many different molds but was first isolated as an antimicrobial active principle during the 1940s from Penicillium patulum (later called Penicillium urticae, now Penicillium griseofulvum). The same metabolite was also isolated from other species and given the names clavacin, claviformin, expansin, mycoin c, and penicidin ( 45). The chemical structure is shown in Fig. 6. A number of early studies were directed towards harnessing its antibiotic activity. For example, it was tested as both a nose and throat spray for treating the common cold and as an ointment for treating fungal skin infections ( 44, 45). However, during the 1950s and 1960s, it became apparent that, in addition to its antibacterial, antiviral, and antiprotozoal activity, patulin was toxic to both plants and animals, precluding its clinical use as an antibiotic. During the 1960s, patulin was reclassified as a mycotoxin. Nowadays , Penicillium expansum, the blue mold that causes soft rot of apples, pears, cherries, and other fruits, is recognized as one of the most common offenders in patulin contamination. Patulin is regularly found in unfermented apple juice, although it does not survive the fermentation into cider products ( 260). Patulin is toxic at high concentration in laboratory settings, but evidence for natural poisoning is indirect and inconclusive. Nevertheless, the Joint Food and Agriculture Organization-World Health Organization Expert Committee on Food Additives has established a provisional maximum tolerable daily intake for patulin of 0.4 mg/kg of body weight per day ( 260). Patulin has also played an important role in the study of the classical biochemistry of polyketide biosynthesis. The first cell extract for a fungal polyketide synthase involved studies on a 6-methylsalicylic acid synthase from the species then called Penicillium urticae (now Penicillium griseofulvum); see Bentley and Bennett ( 13) for a review. Trichothecenes The trichothecenes constitute a family of more than sixty sesquiterpenoid metabolites produced by a number of fungal genera, including Fusarium, Myrothecium, Phomopsis, Stachybotrys, Trichoderma, Trichothecium, and others ( 49, 227, 261). The term trichothecene is derived from trichothecin, which was the one of the first members of the family identified. All trichothecenes contain a common 12,13-epoxytrichothene skeleton and an olefinic bond with various side chain substitutions. They are commonly found as food and feed contaminants, and consumption of these mycotoxins can result in alimentary hemorrhage and vomiting; direct contact causes dermatitis ( 8, 133, 165). Trichothecenes are classified as macrocylic or nonmacrocyclic, depending on the presence of a macrocylic ester or an ester-ether bridge between C-4 and C-15 ( 40). The nonmacrocylic trichothecenes in turn can be subclassified into two groups: type A, which have a hydrogen or ester type side chain at the C-8 position, and include T-2 toxin (Fig. 7), neosolaniol, and diacetoxyscirpenol, while the type B group contain a ketone and include fusarenon-x, nivalenol, and deoxynivalenol (Fig. 8). Fusarium is the major genus implicated in producing the nonmacrocylic trichothecenes. Many members of this genus are significant plant pathogens; their convoluted taxonomy has already been mentioned ( 165). The trichothecenes are extremely potent inhibitors of eukaryotic protein synthesis; different trichothecenes interfere with initiation, elongation, and termination stages. Trichodermin was the first trichothecene shown to inhibit peptidyl transferase activity ( 244, 275). Subsequently, it would appear that while all trichothecenes inhibit peptidyl transferase by binding to the same ribosome-binding site ( 79), they exert different effects which can be correlated with different functional groups. The 12,13-epoxide group is essential for inhibition of protein synthesis; reduction of the 9,10 double bond reduces toxicity ( 173). There is a long history of moldy grain “intoxications” in Japan, where disease in both human beings and farm animals has been attributed to Fusarium mycotoxicoses. Fusarium graminearum (teleomorph Gibberella zeae), regularly found on barley, oats, rye, and wheat, is considered the most important plant pathogen in Japan and is believed to be the cause of red mold disease (Akakabi toxicosis) ( 227, 261). As with all mycotoxins, depending on weather conditions, the growth of trichothecene-producing fungi and subsequent production of toxins vary considerably from year to year and from place to place ( 42). Diacetoxyscirpenol, deoxynivalenol, and T-2 are the best studied of the trichothecenes produced by Fusarium species. Deoxynivalenol is one of the most common mycotoxins found in grains. When ingested in high doses by agricultural animals, it causes nausea, vomiting, and diarrhea; at lower doses, pigs and other farm animals exhibit weight loss and food refusal ( 218). For this reason, deoxynivalenol is sometimes called vomitoxin or food refusal factor. Although less toxic than many other major trichothecenes, it is the most prevalent and is commonly found in barley, corn, rye, safflower seeds, wheat, and mixed feeds ( 178). The symptoms produced by various trichothecenes include effects on almost every major system of the vertebrate body; many of these effects are due to secondary processes that are initiated by often poorly understood metabolic mechanisms related to the inhibition of protein synthesis. A valiant attempt to compile and interpret the data for different trichothecenes, studied in different organisms, administered by different routes, at different doses, at dissimilar intervals, is given by Beasley ( 8). Of the naturally occurring trichothecenes, T-2 and diacetoxyscirpenol appear to be the most potent in animal studies. In addition to their cytotoxic activity, they have an immunosuppressive effect that results in decreased resistance to infectious microbes ( 196, 218). They cause a wide range of gastrointestinal, dermatological, and neurologic symptoms; see Trenholm et al. ( 258) for a summary of 50% lethal dose and other effects in a wide range of experimental and agricultural animals. It has been hypothesized that T-2 and diacetoxyscirpenol are associated with a human disease called alimentary toxic aleukia. The symptoms of the disease include inflammation of the skin, vomiting, and damage to hematopoietic tissues. The acute phase is accompanied by necrosis in the oral cavity, bleeding from the nose, mouth, and vagina, and central nervous system disorders. It is possible that alimentary toxic aleukia may sometimes have been misdiagnosed as diphtheria or scurvy ( 171). The Soviet literature contains many reports of disease outbreaks associated with eating moldy grain, some dating back to the 19th century. Alimentary toxic aleukia affected a large population in the Orenburg district of the former U.S.S.R. during World War II. The sick people had eaten overwintered grain colonized with Fusarium sporotrichioides and Fusarium poae ( 132, 155). The work on alimentary toxic aleukia has been reviewed in detail by Joffe ( 133) and by Beadall and Miller ( 7). Likewise, Matossian ( 171) analyzed patterns of weather and mortality from Russian data for 1861 to 1913 and concluded that low April temperatures (taken as a predictor of T-2 toxin in overwintered and stored grain) were predictive of increased mortality the following summer. It has been pointed out that the hypothesis for an alimentary toxic aleukia-trichothecene connection would be strengthened if T-2 toxin were actually detected in samples of overwintered grain associated with alimentary toxic aleukia outbreaks ( 165) There has been little recent study of alimentary toxic aleukia, but mention of the disease is a fixture of mycotoxin reviews that emphasize human health. More research is needed. The macrocyclic trichothecenes are produced largely by Myrothecium, Stachybotrys, and Trichothecium species. Glutinosin, a mixture of the macrocyclic trichothecenes verrucarin A and B, was originally identified as an antimicrobial agent ( 96). Recently, the trichothecenes produced by Stachybotrys atra ( Stachybotrys chartarum) have received the most attention. They include satratoxins (Fig. 9), roridins, verrucarins, and atranones ( 116). Stachybotryotoxicosis was first described as an equine disease of high mortality associated with moldy straw and hay. Much of the early work on Stachybotrys toxins was published in the Russian literature, focused on horses, and has been well summarized by Forgacs ( 83). Until recently, human stachybotryotoxicosis was considered a rare occupational disease limited largely to farm workers who handle moldy hay ( 117). However, it has become apparent that Stachybotrys grows well on all sorts of wet building materials with high cellulose content, for example, water-damaged gypsum board, ceiling tiles, wood fiber boards, and even dust-lined air conditioning ducts ( 55, 188, 189). The presence of Stachybotrys has been associated with pulmonary bleeding in infants ( 36, 77). Although toxic mold was found in the homes of the children with pulmonary bleeding, a cause-and-effect relationship has been difficult to prove ( 86, 134). Nevertheless, this incident and a few other highly publicized mold cases seem to have precipitated a series of multimillion-dollar law suits against contractors, real estate developers, and insurance companies as well as a great deal of public apprehension about “toxic molds.” See the section on sick building syndrome below for more on this aspect of mycotoxicology. It was suggested that the 10th plague visited on Egypt was a Stachybotrys infestation, but since the plague infected grain, not hay, it is more likely that Aspergillus or Penicillium was the culprit ( 224). Many of the genes involved in trichothecene biosynthesis in Fusarium sporotrichioides and in Myrothecium roridum have been cloned and sequenced ( 34, 61, 206), and there is also considerable progress in understanding the regulation of the trichothecene gene cluster ( 253). Homologues of these trichothecene pathway genes have also been reported for Fusarium graminearum ( 24). Zearalenone Zearalenone (6-[10-hydroxy-6-oxo- trans-1-undecenyl]-B-resorcyclic acid lactone), a secondary metabolite from Fusarium graminearum (teleomorph Gibberella zeae) was given the trivial name zearalenone as a combination of G. zeae, resorcylic acid lactone, -ene (for the presence of the C-1′ to C-2 double bond), and -one, for the C-6′ ketone ( 263). Almost simultaneously, a second group isolated, crystallized, and studied the metabolic properties of the same compound and named it F-2 ( 38, 180). Much of the early literature uses zearalenone and F-2 as synonyms; the family of analogues are known as zearalenones and F-2 toxins, respectively. The structure of zearalenone is given in Fig. 10. Perhaps because the original work on these fungal macrolides coincided with the discovery of aflatoxins, chapters on zearalenone have become a regular fixture in monographs on mycotoxins (see, for example, Mirocha and Christensen [ 179] and Betina [ 15]). Nevertheless, the word toxin is almost certainly a misnomer because zearalenone, while biologically potent, is hardly toxic; rather, it sufficiently resembles 17β-estradiol, the principal hormone produced by the human ovary, to allow it to bind to estrogen receptors in mammalian target cells ( 147) Zearalenone is better classified as a nonsteroidal estrogen or mycoestrogen. Sometimes it is called a phytoestrogen. For the structure-activity relationships of zearalenone and its analogues, see Hurd ( 124) and Shier ( 233). The zearalenones are biosynthesized through a polyketide pathway by Fusarium graminearum, Fusarium culmorum, Fusarium equiseti, and Fusarium crookwellense. All these species are regular contaminants of cereal crops worldwide ( 97). An association between moldy grain consumption and hyperestrogenism in swine has been observed since the 1920s; modern work shows that dietary concentrations of zearalenone as low as 1.0 ppm may lead to hyperestrogenic syndromes in pigs; higher concentrations can lead to disrupted conception, abortion, and other problems ( 148). Reproductive problems have also been observed in cattle and sheep ( 73). The reduced form of zearalenone, zearalenol, has increased estrogenic activity. A synthetic commercial formulation called zeranol (Ralgro) has been marketed successfully for use as an anabolic agent for both sheep and cattle ( 53, 119). In 1989, zeranol was banned by the European Union, but it is still used in other parts of the world ( 97). Zearalenone has also been used to treat postmenopausal symptoms in women ( 264), and both zearelanol and zearalenone have been patented as oral contraceptives ( 115). It has been claimed that the high frequency of early menarche in Puerto Rico might be due to zearalenone and related compounds in the human diet ( 225); however, studies by the Food and Drug Administration do not support this hypothesis ( 147). Recently, endocrine (hormone) disrupters have received a lot of public attention and are widely believed to reduce male fertility in human and wildlife populations ( 106) but it is not clear how much the zearalenones contribute to the total environmental load of xenoestrogens ( 233). Sometimes, hormone disrupters are labeled environmental toxicants, further muddying the distinction between a compound that can cause death (toxin) and a compound that has other pharmacological activities. In summary, the zearalenone family of metabolites illustrates some of the limitations of scientific language. The biological potency of these compounds is high, but the actual toxicity is low. The 50% lethal dose in female rats is greater than 10,000 mg/kg; in female guinea pigs it is 5,000 mg/kg ( 115), while as little as one μg/kg may create a detectable uterogenic response in female swine. Thus, mycoestrogen is a more appropriate rubric than mycotoxin. Moreover, like the ergot alkaloids, in certain formulations some analogues of these macrolides can be called drugs. Extensive reviews of Canadian and Scandinavian epidemiological data have concluded that the risk to human populations is minimal. The recommended safe human intake of zearalenone is estimated to be 0.05 μg/kg of body weight per day ( 147). Zearalenone levels in foodstuffs are not yet regulated anywhere ( 97). Nevertheless, because of its genuine biological potency and regular dietary cooccurrence with true mycotoxins such as the fumonisins and trichothecenes, it would not be prudent to ignore the potential of zearalenone to cause adverse health effects. Other Mycotoxins and Purported Mycotoxicoses Penicillium roqueforti and Penicillium camemberti (= Penicillium caseicola), species used to manufacture mold-ripened cheeses, produce a number of toxic metabolites, including penicillin acid, roquefortine, isoflumigaclavines A and B, PR toxin, and cyclopiazonic acid ( 228). Several mycotoxins induce tremors as a neurological response in farm animals; most of these fungal tremorgens contain a modified indole moiety and are produced by certain species of Aspergillus, Penicillium, and Claviceps. The tremorgenic mycotoxins include the penitrems, janthitrems, lolitrems, aflatrem, paxilline, paspaline, paspalicine, paspalinine, and paspalitrem A and B ( 247). Penicillium crustosum produces penitrem A, a compound implicated in several cases of canine intoxication and one case of human tremor, vomiting, and bloody diarrhea ( 53, 118). Originally isolated from Penicillium cyclopium (now Penicillium aurantiogriseum), cyclopiazonic acid is an indole tetramic acid. This mycotoxin is a specific inhibitor of calcium-dependent ATPase and induces alterations in ion transport across cell membranes ( 214). It is produced by many other species of Penicillium as well as several species of Aspergillus, including Aspergillus flavus. Cyclopiazonic acid was isolated from a sample of the ground nut meal that had been implicated in the original turkey X disease and may have contributed to the severity of that early aflatoxicosis. Furthermore, consumption of a kodo millet that was heavily contaminated with molds and contained detectable levels of cyclopiazonic acid produced kuduo poisoning, characterized by giddiness and nausea ( 209). Finally, some strains of Penicillium camembertii involved in the production of gourmet cheese produce cyclopiazonic acid ( 201). The yellow rice toxins (citrinin, citreoviridin, luteoskyrin, rugulosin, rubroskyrin, and related compounds) are believed to have exacerbated Shoshin-kakke, a particularly malignant form of beriberi seen in Japan in the early 20th century ( 222). A number of rare and obscure diseases have been hypothesized to be possible mycotoxicoses, often on extremely meager evidence. These include Kashin-Beck disease in Russia, mselini joint disease and onylalai in Africa, endemic familial arthritis of Malnad in India, frontoethmoidal encephalomeningocele in Myanmar, sago hemolysis in Papua New Guinea, and deteriorated sugar cane poisoning in China. Descriptions of these conditions and their purported association with exposure to toxigenic fungi have been admirably summarized by Beardall and Miller ( 7). |
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