ST0179

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

53 ESTs from this library were grouped into 47 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
3	56604		Hs.181368	PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae).
2	37736		Hs.558468	Prenyl (decaprenyl) diphosphate synthase, subunit 1.
2	37736		Hs.533122	Splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila).
2	37736		Hs.388668	Kelch-like 2, Mayven (Drosophila).
2	37736		Hs.179986	Flotillin 1.
1	18868		Hs.74137	Transmembrane emp24-like trafficking protein 10 (yeast).
1	18868		Hs.718504	Mitochondrial ribosomal protein S22.
1	18868		Hs.716396	Heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa).
1	18868		Hs.715930	Gastric cancer up-regulated-2.
1	18868		Hs.715594	Peptidylprolyl isomerase G (cyclophilin G).
1	18868		Hs.673372	Transcribed locus, strongly similar to NP_001129575.1 transmembrane protein 132C [Homo sapiens].
1	18868		Hs.666067	Transcribed locus.
1	18868		Hs.633792	Transcribed locus.
1	18868		Hs.631733	G protein-coupled receptor, family C, group 5, member A.
1	18868		Hs.591680	Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating).
1	18868		Hs.583805	MAK16 homolog (S. cerevisiae).
1	18868		Hs.575631	Tripartite motif-containing 14.
1	18868		Hs.562389	Transcribed locus.
1	18868		Hs.555731	Phosphorylated adaptor for RNA export.
1	18868		Hs.519718	Tetratricopeptide repeat domain 1.
1	18868		Hs.519557	Transmembrane protein 14C.
1	18868		Hs.51891	Hect domain and RLD 4.
1	18868		Hs.517307	Myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse).
1	18868		Hs.514342	PAP associated domain containing 5.
1	18868		Hs.511757	Gap junction protein, beta 6, 30kDa.
1	18868		Hs.504237	STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae).
1	18868		Hs.502883	Squamous cell carcinoma antigen recognized by T cells.
1	18868		Hs.491060	Ash1 (absent, small, or homeotic)-like (Drosophila).
1	18868		Hs.480876	Anaphase promoting complex subunit 10.
1	18868		Hs.476209	Plexin B1.
1	18868		Hs.475125	Ataxin 10.
1	18868		Hs.472558	ERGIC and golgi 3.
1	18868		Hs.467084	Eukaryotic translation initiation factor 4 gamma, 3.
1	18868		Hs.437474	RIO kinase 1 (yeast).
1	18868		Hs.436687	SET nuclear oncogene.
1	18868		Hs.433702	Eukaryotic translation initiation factor 5.
1	18868		Hs.390736	CASP8 and FADD-like apoptosis regulator.
1	18868		Hs.282901	RNA binding motif protein 39.
1	18868		Hs.258855	Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila).
1	18868		Hs.205627	Rearranged L-myc fusion.
1	18868		Hs.193613	Transportin 3.
1	18868		Hs.184233	Heat shock 70kDa protein 9 (mortalin).
1	18868		Hs.15535	Ribosomal protein L11.
1	18868		Hs.136947	RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)).
1	18868		Hs.136164	TSPY-like 2.
1	18868		Hs.1116	Lymphotoxin beta receptor (TNFR superfamily, member 3).
1	18868		Hs.104925	Ectodermal-neural cortex (with BTB-like domain).

Source: NCBI UniGene

EST Sequences

150 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• stomach
• gastrointestinal tumor
• adult

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).