Crowe S, Elbeik T, McManus N, Lekas P, Mills J, McGrath M; International Conference on AIDS.

Int Conf AIDS. 1989 Jun 4-9; 5: 639 (abstract no. Th.C.P.137).

San Francisco General Hospital/University of California, San Francisco, California, USA

OBJECTIVE: To compare the techniques of ICF and ISH in assessment of proportion of mphi which contain HIV antigen and HIV RNA respectively. METHODS: Mphi were isolated from (1) peripheral blood (PB) of HIV seronegative donors (n=14) infected in vitro with HIV-DV at multiplicity of one; (2) PB of HIV-infected individuals (n=6). Mphi were stained with Mab directed against HIV p24 (provided by J. Carlson, U.C. Davis) and the proportion of cells expressing HIV was quantitated using an Ortho IIS cytofluorograph. Parallel samples of mphi were spotted onto slides, fixed and ISH was performed with 35S-labelled full-length HIV-SF2 gene probe (provided by Chiron, CA). Slides were coded and examined by a blinded observer. RESULTS: In the 20 samples examined, the proportion of mphi containing HIV antigen varied from 0-70% and the proportion of cells containing RNA varied from 0.001 - 88%. Linear regression analysis showed a strong correlation between the two methods (r=0.77). The calculated relationship between ICF and ISH is: % mphi HIV positive (ISH) = 0.8 [% mphi p24 positive (ICF)]- 3.75. CONCLUSION: ICF and ISH correlate well in determining the proportion of mphi within a population which contain HIV p24 antigen or HIV RNA respectively.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Evaluation Studies
• HIV
• HIV Antibodies
• HIV Core Protein p24
• HIV Envelope Protein gp120
• HIV Infections
• HIV Seropositivity
• In Situ Hybridization
• In Vitro
• RNA Probes
• immunology

Other ID:

• 00338989

UI: 102179291

From Meeting Abstracts