Array Number: 12906624 Total RNA was extracted from Drosophila third instar larvae using Trizol Reagent (Invitrogen). Please refer to Osborne et al. 1997 (PMID: 9242616) for information on the fly strains. 80ug of RNA, quantified by A260 using a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE), was used in each direct labeling reaction (see <http://www.flyarrays.com>) to generate fluorescently labeled cDNA. The respective Cy3 and Cy5 targets were suspended in 75 uL DIG EasyHyb solution (Roche, Mississauga, ON) and were competitively hybridized to the array for 17.75 hours in a CMT-GAPS hybridization chamber (Corning, Acton, MA). Scanning was performed with a ScanArray 4000 system (Perkin Elmer, Boston, MA) using the following settings: 10uM scan: Cy3(543 nm) 100-74, Cy5(633 nm) 100-64 (laser power-PMT Gain). Quantification was performed using QuantArray v3 software (Perkin Elmer) using the adaptive algorithm without background subtraction. The data in the file is not normalized. The ratio in the Value column was derived from normalized data (not shown) as discussed in the manuscript. Keywords = Drosophila Keywords = foraging Keywords = behavior Keywords = cGMP Keywords = guanylyl cyclase Keywords = genetic background Lot batch = D12K_300404
