RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus peru
FDA Seafood List Common Name: Pacific Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19970130gel08
Unique Web Code: psh01Srr
Tissue Aliquot Code: lutjperu I94-002 #09p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	51	6.4	0.100423	4	0.696869	5.7	4.4	4.55	51.1	4	0.0972506
2	5.2	134	16.7	0.145683	4	1.05251	8.6	6.6	5.2	134.3	4.2	0.142649
3	5.85	224	28	0.183951	4	1.16855	9.5	7.3	5.85	224	3.6	0.18198
4	6.55	358	44.7	0.122508	10	2.09218	17	13.1	6.55	356.7	10.3	0.114668
5	6.85	396	49.4	0.075081	4	0.565788	4.6	3.5	6.85	395.8	4.5	0.0717319
6	7.35	469	58.6	0.0985225	5	0.823743	6.7	5.1	7.35	468.9	5	0.0938106
7	8.15	602	75.2	0.140733	7	1.77055	14.4	11.1	8.15	601.8	7.4	0.135579
8	8.45	673	84	0.149403	7	1.80952	14.7	11.3	8.45	672.4	6.9	0.147242
9	8.65	726	90.6	0.117012	4	0.823402	6.7	5.1	8.65	725.1	4	0.11539
10	9.3	783	97.8	0.126132	7	1.47533	12	9.2	9.3	781.3	7.4	0.111855

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.365	25	3.1	0.0657772	10	1.03045	2.5	2.4	4.365	24.5	9.9	0.0588343
2	4.886	94	11.7	0.0230989	4	0.161293	0.4	0.4	4.886	94.3	4.4	0.0208094
3	5.06	117	14.6	0.0933215	4	0.497211	1.2	1.1	5.06	117.2	3.9	0.072798
4	5.246	142	17.7	0.0241886	17	0.808124	1.9	1.8	5.246	127.4	17	0.02689
5	5.365	159	19.9	0.077633	3	0.387632	0.9	0.9	5.365	159.2	2.8	0.0772123
6	5.527	182	22.7	0.0478221	14	1.32905	3.2	3	5.527	192.3	13.9	0.0538312
7	5.681	204	25.5	0.259056	5	1.9482	4.7	4.5	5.681	203.6	4.8	0.230575
8	5.808	222	27.7	0.0128522	3	0.0506747	0.1	0.1	5.808	223.4	3.1	0.0090797
9	5.902	238	29.7	0.0335871	5	0.279529	0.7	0.6	5.902	237.6	4.7	0.0337032
10	6.012	259	32.3	0.599802	4	3.75604	9	8.6	6.012	259.3	3.6	0.580823
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.106	277	34.6	0.350015	4	2.22337	5.3	5.1	6.106	276.7	3.7	0.335949
12	6.205	296	37	0.267248	5	2.50683	6	5.7	6.205	295	5.3	0.26687
13	6.289	312	39	0.0393638	4	0.259982	0.6	0.6	6.289	312.4	3.6	0.04106
14	6.362	326	40.7	0.123096	3	0.64934	1.6	1.5	6.362	326.4	2.9	0.12643
15	6.451	343	42.8	0.0769913	8	1.51775	3.6	3.5	6.451	347.1	8.4	0.102175
16	6.508	354	44.2	0.224529	2	0.765191	1.8	1.8	6.508	354.5	2.4	0.182003
17	6.559	363	45.3	0.0456704	2	0.130874	0.3	0.3	6.559	364.3	1.7	0.0444758
18	6.661	375	46.8	0.550009	4	3.65048	8.7	8.3	6.661	375	3.9	0.532297
19	6.764	387	48.3	0.513016	2	2.00632	4.8	4.6	6.764	387.4	2.3	0.497694
20	6.864	399	49.8	0.230353	8	3.05599	7.3	7	6.864	400	7.8	0.220547
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.265	456	56.9	0.213297	3	0.814632	1.9	1.9	7.265	456	2.6	0.179535
22	7.35	468	58.4	0.0632151	9	1.21136	2.9	2.8	7.35	464.1	9	0.076065
23	7.572	505	63	0.0085709	3	0.0484759	0.1	0.1	7.572	504.2	3.3	0.0082732
24	7.668	521	65	0.0411093	5	0.367167	0.9	0.8	7.668	519.6	5.1	0.0402609
25	7.805	544	67.9	0.359184	5	2.90118	6.9	6.6	7.805	544.4	4.8	0.341406
26	7.985	574	71.7	0.240054	15	8.37373	20	19.2	7.985	580.5	14.6	0.323643
27	9.232	775	96.8	0.0624794	10	1.07614	2.6	2.5	9.232	774	10	0.0607359

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	48	6	0.125485	5	0.977144	8.7	6.3	4.55	48.4	4.7	0.117454
2	5.2	137	17.1	0.122468	5	1.16419	10.4	7.5	5.2	135.8	5.4	0.122237
3	5.85	232	29	0.154497	4	1.01307	9	6.6	5.85	231.7	3.8	0.150447
4	6.55	366	45.7	0.132877	9	1.90406	17	12.3	6.55	365	8.8	0.12257
5	6.85	398	49.7	0.0744127	5	0.569616	5.1	3.7	6.85	398.1	4.5	0.0707407
6	7.35	467	58.3	0.0938823	5	0.775789	6.9	5	7.35	466.4	4.9	0.0892839
7	8.15	601	75	0.134924	7	1.60436	14.3	10.4	8.15	600.8	6.9	0.131691
8	8.45	666	83.1	0.1074	8	1.47879	13.2	9.6	8.45	666.3	7.9	0.105181
9	8.65	721	90	0.0973857	5	0.814234	7.3	5.3	8.65	721.2	4.8	0.0956995
10	9.3	779	97.3	0.10825	5	0.920742	8.2	6	9.3	778.4	5.1	0.101619

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.32	24	3	0.0454126	7	0.515796	1.5	1.5	4.32	23	7.5	0.0389203
2	4.55	53	6.6	0.0742314	4	0.486621	1.4	1.4	4.55	52.8	3.7	0.0735981
3	4.891	96	12	0.0176641	5	0.151169	0.4	0.4	4.891	95.5	4.8	0.0177151
4	5.065	118	14.8	0.0449665	6	0.479444	1.4	1.4	5.065	118.6	6.2	0.0433563
5	5.2	135	16.9	0.067824	6	0.69974	2.1	2	5.2	134.5	5.8	0.0675463
6	5.315	152	19	0.0403414	6	0.415685	1.2	1.2	5.315	152.6	5.8	0.0403297
7	5.505	180	22.5	0.0248735	16	0.916048	2.7	2.6	5.505	192.4	16.4	0.0315047
8	5.667	204	25.5	0.182626	5	1.52178	4.5	4.4	5.667	203.9	5.2	0.164662
9	5.85	231	28.9	0.0719322	6	0.650385	1.9	1.9	5.85	231	5.5	0.0663364
10	6.019	263	32.9	0.361994	5	3.22875	9.5	9.3	6.019	261.9	4.9	0.374053
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.104	279	34.9	0.224887	5	2.05539	6.1	5.9	6.104	278.6	5.2	0.223173
12	6.194	296	37	0.226141	5	2.19573	6.5	6.3	6.194	296.2	5.5	0.226924
13	6.284	313	39.2	0.0432917	3	0.24994	0.7	0.7	6.284	313.5	3.3	0.0433343
14	6.358	327	40.9	0.0735375	3	0.365984	1.1	1	6.358	327	2.8	0.0732243
15	6.427	340	42.6	0.0313678	10	1.27686	3.8	3.7	6.427	349.2	10.4	0.0691747
16	6.506	355	44.4	0.220168	2	0.648373	1.9	1.9	6.506	355	2.1	0.170281
17	6.575	366	45.8	0.0315114	1	0	0	0	6.575	360	1	0
18	6.668	376	47.1	0.513751	4	3.12007	9.2	9	6.668	376.2	3.6	0.492698
19	6.788	389	48.7	0.436872	2	1.65158	4.9	4.7	6.788	388.8	2.2	0.41965
20	6.881	400	50.1	0.159661	7	1.97989	5.8	5.7	6.881	400.4	7.2	0.155052
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.286	457	57.2	0.163548	3	0.891952	2.6	2.6	7.286	457.3	3.2	0.15575
22	7.35	466	58.3	0.129414	5	1.12872	3.3	3.2	7.35	465.7	4.9	0.129816
23	7.576	503	63	0.0121968	5	0.0975886	0.3	0.3	7.576	502.6	5.2	0.0106671
24	7.674	519	65	0.0337721	5	0.257334	0.8	0.7	7.674	518.6	4.5	0.0319883
25	7.808	541	67.7	0.308109	4	2.22687	6.6	6.4	7.808	541	4.1	0.30345
26	7.985	570	71.3	0.212535	12	4.15567	12.3	11.9	7.985	572.8	12	0.195926
27	8.45	669	83.7	0.0636815	5	0.616439	1.8	1.8	8.45	669	5.5	0.0635497
28	8.65	721	90.2	0.0623307	4	0.475207	1.4	1.4	8.65	720.6	4.3	0.0620418
29	9.3	775	97	0.0823769	11	1.39321	4.1	4	9.3	771.4	10.5	0.0747681

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.351	23	2.9	0.0840996	6	0.875457	2.1	2	4.351	22.7	6.3	0.0788094
2	4.951	100	12.5	0.0197596	5	0.218889	0.5	0.5	4.951	97.3	5.3	0.0232268
3	5.099	119	14.9	0.102945	5	0.836584	2	1.9	5.099	119.1	5.3	0.0889855
4	5.259	141	17.6	0.0212004	15	0.641543	1.6	1.5	5.259	132.4	14.8	0.0244699
5	5.385	160	20	0.0669713	4	0.389275	0.9	0.9	5.385	159.9	3.6	0.0617571
6	5.563	187	23.4	0.0439016	16	1.45023	3.5	3.4	5.563	196.9	16.3	0.0502858
7	5.715	210	26.3	0.188643	5	1.46447	3.6	3.4	5.715	209	5.1	0.161643
8	5.853	231	28.9	0.0114548	2	0.02721	0.1	0.1	5.853	231.2	2.5	0.0061977
9	5.927	245	30.7	0.0243432	4	0.192841	0.5	0.4	5.927	245.2	4.5	0.0242066
10	6.038	266	33.3	0.578718	4	3.95308	9.6	9.2	6.038	266	3.9	0.57085
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.117	281	35.2	0.348691	4	2.24456	5.5	5.2	6.117	280.8	3.8	0.334346
12	6.197	296	37	0.302925	5	2.48528	6	5.8	6.197	295.6	4.6	0.305485
13	6.266	309	38.7	0.0502663	3	0.249092	0.6	0.6	6.266	310.1	2.8	0.0494077
14	6.34	323	40.4	0.135406	3	0.625824	1.5	1.5	6.34	322.6	2.6	0.136766
15	6.425	339	42.4	0.0818632	8	1.60081	3.9	3.7	6.425	342.6	8.4	0.107499
16	6.488	351	43.9	0.273278	2	0.884188	2.2	2.1	6.488	350.9	2.1	0.234763
17	6.553	363	45.4	0.0382959	1	0	0	0	6.553	357	1	0
18	6.654	374	46.8	0.53823	3	3.21942	7.8	7.5	6.654	373.8	3.5	0.520294
19	6.783	388	48.6	0.475971	2	1.70605	4.1	4	6.783	387.7	2	0.486071
20	6.883	400	50.1	0.215758	7	2.69102	6.5	6.2	6.883	401.3	7.4	0.204099
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.278	455	56.9	0.224412	5	1.71447	4.2	4	7.278	455.6	5	0.194785
22	7.572	501	62.7	0.0097081	3	0.050112	0.1	0.1	7.572	500.3	3.3	0.0086672
23	7.658	515	64.5	0.0364364	4	0.304709	0.7	0.7	7.658	514.7	4.5	0.0384042
24	7.805	539	67.5	0.347897	5	2.87903	7	6.7	7.805	538.5	4.9	0.333715
25	7.972	566	70.8	0.21372	18	9.5916	23.3	22.3	7.972	576.6	18	0.300643
26	9.225	769	96.2	0.0400338	12	0.814658	2	1.9	9.225	768.2	12.4	0.0371795

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	44	5.5	0.114306	6	1.24999	10.3	7.7	4.55	44.9	6.5	0.108707
2	5.2	129	16.1	0.106566	6	1.22258	10	7.6	5.2	131.2	6.5	0.106132
3	5.85	230	28.8	0.175375	4	1.1664	9.6	7.2	5.85	229.5	3.7	0.176903
4	6.55	362	45.3	0.126543	8	1.66122	13.7	10.3	6.55	361.2	7.7	0.12129
5	6.85	395	49.4	0.10114	5	0.820647	6.7	5.1	6.85	395.9	4.9	0.0948383
6	7.35	464	58.1	0.12378	4	0.947494	7.8	5.9	7.35	463.4	4.5	0.118978
7	8.15	593	74.2	0.120209	7	1.39439	11.5	8.6	8.15	593	6.6	0.119705
8	8.45	662	82.9	0.135842	7	1.61938	13.3	10	8.45	661.4	6.9	0.132615
9	8.65	717	89.7	0.107822	4	0.822669	6.8	5.1	8.65	716.2	4.3	0.106872
10	9.3	776	97.1	0.0674236	14	1.26388	10.4	7.8	9.3	771.7	13.7	0.0520425

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 112

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.700 5.200 8.600 5.850 9.500 6.550 17.000 6.850 4.600 7.350 6.700 8.150 14.400 8.450 14.700 8.650 6.700 9.300 12.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.365 2.500 4.886 0.400 5.060 1.200 5.246 1.900 5.365 0.900 5.527 3.200 5.681 4.700 5.808 0.100 5.902 0.700 6.012 9.000 6.106 5.300 6.205 6.000 6.289 0.600 6.362 1.600 6.451 3.600 6.508 1.800 6.559 0.300 6.661 8.700 6.764 4.800 6.864 7.300 7.265 1.900 7.350 2.900 7.572 0.100 7.668 0.900 7.805 6.900 7.985 20.000 9.232 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 27 Return to Lane Navigator
4.550 8.700 5.200 10.400 5.850 9.000 6.550 17.000 6.850 5.100 7.350 6.900 8.150 14.300 8.450 13.200 8.650 7.300 9.300 8.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.320 1.500 4.550 1.400 4.891 0.400 5.065 1.400 5.200 2.100 5.315 1.200 5.505 2.700 5.667 4.500 5.850 1.900 6.019 9.500 6.104 6.100 6.194 6.500 6.284 0.700 6.358 1.100 6.427 3.800 6.506 1.900 6.575 0 6.668 9.200 6.788 4.900 6.881 5.800 7.286 2.600 7.350 3.300 7.576 0.300 7.674 0.800 7.808 6.600 7.985 12.300 8.450 1.800 8.650 1.400 9.300 4.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 29 Return to Lane Navigator
4.351 2.100 4.951 0.500 5.099 2.000 5.259 1.600 5.385 0.900 5.563 3.500 5.715 3.600 5.853 0.100 5.927 0.500 6.038 9.600 6.117 5.500 6.197 6.000 6.266 0.600 6.340 1.500 6.425 3.900 6.488 2.200 6.553 0 6.654 7.800 6.783 4.100 6.883 6.500 7.278 4.200 7.572 0.100 7.658 0.700 7.805 7.000 7.972 23.300 9.225 2.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 26 Return to Lane Navigator
4.550 10.300 5.200 10.000 5.850 9.600 6.550 13.700 6.850 6.700 7.350 7.800 8.150 11.500 8.450 13.300 8.650 6.800 9.300 10.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Snapper ---- Go to Display Gel Lane Navigator.