|
Status |
Public on Jul 08, 2008 |
Title |
Induced beta cells 3 |
Sample type |
RNA |
|
|
Source Name |
induced beta cells
|
Organism(s) |
Mus musculus |
Characteristics |
adult mouse GFP+
|
Extracted molecule |
total RNA |
Extraction protocol |
~5000 GFP+ cells, 4-5 islets, or ~10,000 no islet cells per sample was extracted for total RNA with trizol reagent (invitorgen)
|
Label |
biotin
|
Label protocol |
Total prep RNA amplification kit (Ambion)
|
|
|
Hybridization protocol |
standard as recommended by illumina
|
Scan protocol |
standard as recommended by illumina
|
Description |
rag-/- adult pancreas infected with M3 adeno virus 1 month FACS sorted contain 22% ins+ cells
|
Data processing |
with Beadstudio, standardized. Differentially expressed genes identified with illumina recommended parameters: pvalue<0.05, diff score >30, average signal >100
|
|
|
Submission date |
Jul 07, 2008 |
Contact name |
qiao zhou |
E-mail(s) |
qiao@mcb.harvard.edu
|
Phone |
617 495 2918
|
Organization name |
Harvard University
|
Department |
Stem cell and regenerative biology
|
Street address |
7 Divinity Ave
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02143 |
Country |
USA |
|
|
Platform ID |
GPL6103 |
Series (1) |
GSE12025 |
Comparison of endocrine enriched genes in islet beta cells versus induced beta cells |
|