Sato N, Iwata S, Masutani H, Nakamura K, Hori T, Yodoi J; International Conference on AIDS.
Int Conf AIDS. 1994 Aug 7-12; 10: 95 (abstract no. PA0262).
Dept. of Biological Responses, Kyoto Univ., Japan.
OBJECTIVE: Recent evidence suggests that oxidative stress may mediates HIV-induced apoptosis of lymphocytes. We have previously shown that ADF/human thioredoxin (hTrx), an endogenous antioxidant, was downregulated by HIV infection. To study the possible involvement of ADF/thioredoxin in apoptotic process, we have analyzed the effect of thiol modification on a human T lymphoid cell line. METHODS: A human T lymphoid cell line, Jurkat, were treated with either diamide (a sulfhydryl-specific oxidant) or buthionine sulfoximine (BSO; an inhibitor of glutathione synthesis). After drug treatment, we have analyzed 1) intracellular GSH content by calorimetric assay, 2) oxidation state of ADF/hTrx by native-PAGE/immunoblotting, 3) DNA content after detergent extraction by propidium iodide staining/flowcytometry. RESULTS: When cells were treated with diamide, decrease of GSH content, oxidation of ADF/hTrx and induction of apoptosis were observed, whereas the treatment with BSO did not induce apoptosis or oxidation of ADF/hTrx up to 48 hours in spite of marked decrease of GSH content. DISCUSSION AND CONCLUSIONS: Our results implies that oxidation or inactivation of ADF/hTrx may lead to apoptotic cell death. The downregulation of ADF/hTrx may have pathogenic or facilitatory roles in HIV-induced apoptosis. Whether replenishment of ADF/hTrx blocks HIV-induced apoptosis remains to be determined to establish the therapeutic potential of ADF/hTrx for HIV infection.
Publication Types:
Keywords:
- AIDS Vaccines
- Apoptosis
- Buthionine Sulfoximine
- CASP4 protein, human
- Caspases
- Cell Death
- Cytokines
- Diamide
- Down-Regulation
- Fungal Proteins
- Glutathione
- Humans
- Lymphocytes
- Neoplasm Proteins
- Oxidants
- Oxidation-Reduction
- Oxidoreductases
- Sulfhydryl Compounds
- Thioredoxins
- adult T cell leukemia-derived factor
- genetics
Other ID:
UI: 102211388
From Meeting Abstracts