Liang CM, Lian SM, Henry S, Epstein JS; International Conference on AIDS.

Int Conf AIDS. 1990 Jun 20-23; 6: 336 (abstract no. 1088).

Food and Drug Administration, Bethesda, Maryland, USA

OBJECTIVE: To study the effect of glutathione (GSH) on expression of murine CD4 and CD8 in splenic lymphocytes, as a model system. METHODS: Murine splenic lymphocytes were cultured in vitro for 48-72 h with or without GSH or with L-buthionine-(S,R)-sulfoximine (BSO), an inhibitor of de novo GSH synthesis, and then treated with fluorescein isothiocyanate-labeled anti-murine CD8 or phycoerythrin-labeled anti-murine CD4 antibodies. The cells were then analyzed on a FACStar Plus (Becton Dickinson) flow cytometer. The frequency and intensity of the CD4+ and CD8+ cells cultured with and without GSH or BSO were compared. RESULTS: Treatment of murine lymphocytes with GSH (1 mg/ml) increased the cellular GSH level by 20 fold (from 100 ng to 2,000 ng/ 10(6) cells), and this increase was fully inhibited by BSO. GSH treatment increased the population of CD8+-cells by 3-fold (from 3.2% to 10.6%) while untreated cells were the same as BSO treated cells. Although GSH treatment did not significantly increase the population of CD4+-cells (34.2% vs. 36.8%), the fluorescence intensity of CD4+ cells was higher after the treatment (peak channel shift from 550 to 593). CONCLUSION: Elevation of cellular GSH increases the expression of CD4 and CD8 receptors in murine lymphocytes. This result provides indirect support for the hypothesis that depletion of cellular GSH contributes to the immune dysfunction in AIDS.

Publication Types:

• Meeting Abstracts

Keywords:

• Antigens, CD4
• Antigens, CD8
• CD4-Positive T-Lymphocytes
• CD8 receptor
• CD8-Positive T-Lymphocytes
• Cell Adhesion Molecules
• Flow Cytometry
• Glutathione
• In Vitro
• Lymphocytes
• Methionine
• Receptors, Antigen, T-Cell
• Spleen
• buthionine
• immunology
• surgery

Other ID:

• 40108890

UI: 102196939

From Meeting Abstracts