|
| Status |
Public on Jun 15, 2008 |
| Title |
Small intestinal GIST 5 |
| Sample type |
RNA |
| |
|
| Source Name |
Total RNA from human small intestinal GIST.
|
| Organism(s) |
Homo sapiens |
| Characteristics |
Gender: male
Age: 34
Tumor size: 18
KIT mutation: Exon 11
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using IsoGen lysis buffer following manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix human genome HG-U133A genechip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanned on a GeneArray scanner.
Images were quantified using Microarray Analysis Suite version 4.0 software (Affymetrix).
|
| Description |
Frozen tumour specimens from untreated GIST of the stomach or small intestine.
|
| Data processing |
The data were analyzed and normalized using MAS5 algorithm with the ArrayAssist 4.X. software.
|
| |
|
| Submission date |
Jun 18, 2007 |
| Contact name |
Umio Yamaguchi |
| E-mail(s) |
umioy@yahoo.co.jp
|
| Organization name |
NCCRI
|
| Department |
Chemotherapy
|
| Street address |
5-1-1, Tsukiji
|
| City |
Chuo |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8167 |
Distinct gene-expression-defined classes of gastrointestinal stromal tumor (GIST). |
|
