Gray bat - Myotis grisescens (E)
 Indiana bat - Myotis sodalis (E)
 Bald eagle - Haliaeetus leucocephalus (T)
 Snail darter - Percina tanasi (T)
 Pale lilliput pearly mussel - Toxolasma cylindrella (E)(h)
 Dromedary pearly mussel - Dromus dromas (E)(h)
 Pink mucket pearly mussel - Lampsilis abrupta (=Lampsilis orbiculata) (E)
 Rough pigtoe - Pleurobema plenum (E)(h)
 Orangefoot pimpleback - Plethobasus cooperianus (E)(h)
 White wartyback pearly mussel - Plethobasus cicatricosus (E)(h)
 Anthony's snail - Athearnia anthonyi (E)
 Royal (obese) marstonia snail - Marstonia ogmoraphe (E)
 Large-flowered skullcap - Scutellaria montana (E)
 American hart's tongue fern - Asplenium scolependrium (T)
 Price's potato bean - Apios priceana (T)
 Sequatchie caddisfly - Glyphopsyche sequatchie (C)
White fringeless orchid - Platanthera integrilabia (C)