WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.
BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.
BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract
Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract
RepeatMasker repeats found in sequence:No Repeats Found.Reference: Gish, Warren (1994-1997). unpublished. Gish, Warren and David J. States (1993). Identification of protein coding regions by database similarity search. Nat. Genet. 3:266-72.Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.
Query= A12A07_CONSENSUS (508 letters)
Translating both strands of query sequence in all 6 reading framesDatabase: nr 625,274 sequences; 197,782,623 total letters.Observed Numbers of Database Sequences Satisfying Various EXPECTation Thresholds (E parameter values) Histogram units: = 5 Sequences : less than 5 sequences EXPECTation Threshold (E parameter) | V Observed Counts--> 10000 997 291 |========================================================== 6310 706 151 |============================== 3980 555 136 |=========================== 2510 419 118 |======================= 1580 301 124 |======================== 1000 177 65 |============= 631 112 45 |========= 398 67 23 |==== 251 44 12 |== 158 32 10 |== 100 22 3 |: 63.1 19 3 |: 39.8 16 4 |: 25.1 12 0 | 15.8 12 1 |: >>>>>>>>>>>>>>>>>>>>> Expect = 10.0, Observed = 11 <<<<<<<<<<<<<<<<< 10.0 11 0 | 6.31 11 0 | 3.98 11 0 | 2.51 11 0 | 1.58 11 1 |: Smallest Sum Reading High Probability Sequences producing High-scoring Segment Pairs: Frame Score P(N) N gi|7460098|pir||T05076hypothetical protein T6K21.80 -... +2 425 6.9e-39 1 gi|6714272|gb|AAF25968.1|AC017118_5(AC017118) F6N18.8... +2 303 5.8e-26 1 gi|12643058|gb|AAK00447.1|AC060755_17(AC060755) unkno... +2 264 7.9e-22 1 gi|9758507|dbj|BAB08915.1|(AB016882) gene_id:MZA15.12... +2 245 8.1e-20 1 gi|4836888|gb|AAD30591.1|AC007369_1(AC007369) Unknown... +2 203 2.3e-15 1 gi|12323060|gb|AAG51520.1|AC068324_8(AC068324) hypoth... +2 199 6.1e-15 1 gi|12323979|gb|AAG51950.1|AC015450_11(AC015450) unkno... +2 190 5.5e-14 1 gi|4510423|gb|AAD21509.1|(AC006929) unknown protein [... +2 129 1.6e-07 1 gi|11280631|pir||T47878hypothetical protein T4C21.80 ... +2 125 9.5e-07 1 gi|9755373|gb|AAF98180.1|AC000107_3(AC000107) F17F8.4... +2 124 1.2e-06 1 gi|5441885|dbj|BAA82383.1|(AP000367) Similar to Arabi... +2 73 0.74 2 Locally-aligned regions (HSPs) with respect to query sequence: Locus_ID Frame 2 Hits gi|7460098 | _____________________________________ gi|6714272 | ______________________________________________ gi|12643058 | _____________________________________ gi|9758507 | _____________________________________ gi|4836888 | ______________________________ gi|12323060 | ___________________________ gi|12323979 | ____________________________ gi|4510423 | _________________________ gi|11280631 | _____________________________ gi|9755373 | ___________________________ gi|5441885 | ___________ ______________ __________________________________________________ Query sequence: | | | | | 170 0 50 100 150
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>gi|7460098|pir||T05076 hypothetical protein T6K21.80 - Arabidopsis thaliana >gi|2894599|emb|CAA17133.1| (AL021889) putative protein [Arabidopsis thaliana] >gi|7268542|emb|CAB78792.1| (AL161547) putative protein [Arabidopsis thaliana] Length = 254 Frame 2 hits (HSPs): _________________________ __________________________________________________ Database sequence: | | | | | || 254 0 50 100 150 200 250 Plus Strand HSPs: Score = 425 (149.6 bits), Expect = 6.9e-39, P = 6.9e-39 Identities = 78/123 (63%), Positives = 91/123 (73%), Frame = +2 Query: 137 MAIENQDNTVREIKPKNRRIMGAGGPDDDDNRWPPWLKPLLKESFFVQCKLHADSHKSEC 316 MAIE+Q+NT+REIKPKNRRIMGAGGP++++NRWPPWLKPLLKE FFV CK H DSHKSEC Sbjct: 1 MAIEDQENTIREIKPKNRRIMGAGGPEEEENRWPPWLKPLLKEQFFVHCKFHGDSHKSEC 60 Query: 317 NMLLLGLYEWPLCSLCLAHHKDHRAIQIXSPHTMMLIRXSXIQKVFGLTGVQPTLSTAQG 496 NM L PLCSLCLAHHKDHR IQI +IR + IQK + G+Q + + Sbjct: 61 NMYCLDCTNGPLCSLCLAHHKDHRTIQIRRSSYHDVIRVNEIQKYLDIGGIQTYVINSAK 120 Query: 497 XVF 505 VF Sbjct: 121 VVF 123
>gi|6714272|gb|AAF25968.1|AC017118_5 (AC017118) F6N18.8 [Arabidopsis thaliana] Length = 270 Frame 2 hits (HSPs): _____________________________ __________________________________________________ Database sequence: | | | | | | | 270 0 50 100 150 200 250 Plus Strand HSPs: Score = 303 (106.7 bits), Expect = 5.8e-26, P = 5.8e-26 Identities = 74/152 (48%), Positives = 91/152 (59%), Frame = +2 Query: 50 FSSIFLKKQKHEKSSTLSHSLSLSLLSTNMAIE---NQDNTVREIKPKNRRIMGAGGPDD 220 F S+ QK S SL L S +AIE +Q+ T+REIK + A ++ Sbjct: 8 FGSLNPPYQKQGLSQL--RSLGSVLGSKKLAIEENHHQEATIREIKALQHKNQHA---EE 62 Query: 221 DDNR-WPPWLKPLLKESFFVQCKLHADSHKSECNMLLLGLYEWPLCSLCLAHHKDHRAIQ 397 + N+ +P WLKPLL+E FFVQCKLHADSHKSECNM L PLCSLCL+ HKDH AIQ Sbjct: 63 ETNKTYPHWLKPLLREKFFVQCKLHADSHKSECNMYCLDCTNGPLCSLCLSFHKDHHAIQ 122 Query: 398 IXSPHTMMLIRXSXIQKVFGLTGVQPTLSTAQGXVF 505 I +IR S IQK +TGVQ + + VF Sbjct: 123 IRRSSYHDVIRVSEIQKFLDITGVQTYVINSAKVVF 158
>gi|12643058|gb|AAK00447.1|AC060755_17 (AC060755) unknown protein [Oryza sativa] Length = 254 Frame 2 hits (HSPs): ___________________________ __________________________________________________ Database sequence: | | | | | || 254 0 50 100 150 200 250 Plus Strand HSPs: Score = 264 (92.9 bits), Expect = 7.9e-22, P = 7.9e-22 Identities = 62/137 (45%), Positives = 81/137 (59%), Frame = +2 Query: 137 MAIENQDNTVREIKPKNRRIMGAGGPDDDDN-------RWPPWLKPLLKESFFVQCKLHA 295 MAI+++ + +E++ KNRRIMG GGP+ ++ +WP WL PLL SFF QCK+HA Sbjct: 1 MAIDHE-SPFKELRLKNRRIMGGGGPEPEEEEAVAHGEQWPRWLSPLLSASFFSQCKVHA 59 Query: 296 DSHKS-ECNMLLLGLYE------WPLCSLCLAH-HKDHRAIQIXSPHTMMLIRXSXIQKV 451 DSH+S ECNM L LCSLCLAH H+DH IQI +IR S IQ+ Sbjct: 60 DSHRSGECNMFCLDCAADADAAAAALCSLCLAHNHRDHHTIQIRRSSYHDVIRVSDIQRF 119 Query: 452 FGLTGVQPTLSTAQGXVF 505 + GVQ + + VF Sbjct: 120 MDIGGVQTYVINSARVVF 137
>gi|9758507|dbj|BAB08915.1| (AB016882) gene_id:MZA15.12~pir||T05076~similar to unknown protein [Arabidopsis thaliana] Length = 226 Frame 2 hits (HSPs): __________________________ __________________________________________________ Database sequence: | | | | | | 226 0 50 100 150 200 Plus Strand HSPs: Score = 245 (86.2 bits), Expect = 8.1e-20, P = 8.1e-20 Identities = 50/123 (40%), Positives = 69/123 (56%), Frame = +2 Query: 137 MAIENQDNTVREIKPKNRRIMGAGGPDDDDNRWPPWLKPLLKESFFVQCKLHADSHKSEC 316 MAIE+ +N REIKPKNRR M + +N+WP WLKPLL + FF QCK H ++EC Sbjct: 1 MAIEDYENPNREIKPKNRRFM------EGENQWPIWLKPLLNQHFFAQCKFHGHLPRTEC 54 Query: 317 NMLLLGLYEWPLCSLCLAHHKDHRAIQIXSPHTMMLIRXSXIQKVFGLTGVQPTLSTAQG 496 M L CSLCL+ H++HR IQI + + IQK ++ +Q + + Sbjct: 55 KMYCLDCTNDSFCSLCLSEHENHRTIQIRISSYHNVTKVDEIQKYLDISSIQTYVINSSK 114 Query: 497 XVF 505 +F Sbjct: 115 VLF 117
>gi|4836888|gb|AAD30591.1|AC007369_1 (AC007369) Unknown protein [Arabidopsis thaliana] Length = 243 Frame 2 hits (HSPs): ____________________ __________________________________________________ Database sequence: | | | | | | 243 0 50 100 150 200 Plus Strand HSPs: Score = 203 (71.5 bits), Expect = 2.3e-15, P = 2.3e-15 Identities = 38/97 (39%), Positives = 55/97 (56%), Frame = +2 Query: 215 DDDDNRWPPWLKPLLKESFFVQCKLHADSHKSECNMLLLGLYEWPLCSLCLAHHKDHRAI 394 +++D PPWL P+L+ S+FV C +H DS+K+ECN+ L CS CL HKDHR + Sbjct: 6 EEEDYTSPPWLMPMLRGSYFVPCSIHVDSNKNECNLFCLDCAGNAFCSYCLVKHKDHRVV 65 Query: 395 QIXSPHTMMLIRXSXIQKVFGLTGVQPTLSTAQGXVF 505 QI ++R + IQK + VQ + + VF Sbjct: 66 QIRRSSYHNVVRVNEIQKFIDIACVQTYIINSAKIVF 102
>gi|12323060|gb|AAG51520.1|AC068324_8 (AC068324) hypothetical protein [Arabidopsis thaliana] Length = 216 Frame 2 hits (HSPs): ____________________ __________________________________________________ Database sequence: | | | | | | 216 0 50 100 150 200 Plus Strand HSPs: Score = 199 (70.1 bits), Expect = 6.1e-15, P = 6.1e-15 Identities = 37/86 (43%), Positives = 51/86 (59%), Frame = +2 Query: 215 DDDDNRWPPWLKPLLKESFFVQCKLHADSHKSECNMLLLGLYEWPLCSLCLAHHKDHRAI 394 ++DD PPWL P+L+ +FV C +H+ S KSECN+ L CS CLAHH+ HR I Sbjct: 2 ENDDVMTPPWLTPMLRADYFVTCSIHSQSSKSECNLFCLDCSGNAFCSSCLAHHRTHRVI 61 Query: 395 QIXSPHTMMLIRXSXIQKVFGLTGVQ 472 QI ++R S IQK ++ +Q Sbjct: 62 QIRRSSYHNVVRVSEIQKHIDISCIQ 87
>gi|12323979|gb|AAG51950.1|AC015450_11 (AC015450) unknown protein; 77280-78196 [Arabidopsis thaliana] Length = 242 Frame 2 hits (HSPs): ____________________ __________________________________________________ Database sequence: | | | | | | 242 0 50 100 150 200 Plus Strand HSPs: Score = 190 (66.9 bits), Expect = 5.5e-14, P = 5.5e-14 Identities = 35/90 (38%), Positives = 53/90 (58%), Frame = +2 Query: 236 PPWLKPLLKESFFVQCKLHADSHKSECNMLLLGLYEWPLCSLCLAHHKDHRAIQIXSPHT 415 PPWL P+L+ ++F+ C +HA S+KSECNM L CS CL +H++HR +QI Sbjct: 15 PPWLIPMLRANYFIPCSIHAASNKSECNMFCLDCSSEAFCSYCLLNHRNHRVLQIRRSSY 74 Query: 416 MMLIRXSXIQKVFGLTGVQPTLSTAQGXVF 505 ++R + IQK ++ VQ + + VF Sbjct: 75 HNVVRVNEIQKYIDISCVQTYIINSARIVF 104
>gi|4510423|gb|AAD21509.1| (AC006929) unknown protein [Arabidopsis thaliana] Length = 135 Frame 2 hits (HSPs): ______________________________ __________________________________________________ Database sequence: | | | | 135 0 50 100 Plus Strand HSPs: Score = 129 (45.4 bits), Expect = 1.6e-07, P = 1.6e-07 Identities = 27/80 (33%), Positives = 40/80 (50%), Frame = +2 Query: 236 PPWLKPLLKESFFVQCKLHADSHKSECNMLLLGLYEWPLCSLCLAH-HKDHRAIQIXSPH 412 P WL+ LL+ +FF C H ++ ++ECNM L C C + H DH +QI Sbjct: 4 PKWLEGLLRTNFFSICPRHRETPRNECNMFCLSCQNAAFCFYCRSSFHIDHPVLQIRRSS 63 Query: 413 TMMLIRXSXIQKVFGLTGVQ 472 ++R S I+ + GVQ Sbjct: 64 YHDVVRVSEIENALDIRGVQ 83
>gi|11280631|pir||T47878 hypothetical protein T4C21.80 - Arabidopsis thaliana >gi|7329677|emb|CAB82671.1| (AL162295) putative protein [Arabidopsis thaliana] Length = 245 Frame 2 hits (HSPs): ____________________ __________________________________________________ Database sequence: | | | | | | 245 0 50 100 150 200 Plus Strand HSPs: Score = 125 (44.0 bits), Expect = 9.5e-07, P = 9.5e-07 Identities = 29/95 (30%), Positives = 46/95 (48%), Frame = +2 Query: 221 DDNRWPPWLKPLLKESFFVQCKLHADSHKSECNMLLLGLYEWPLCSLCLAHHKDHRAIQI 400 + +P WL+ LLK+ FF C H D K+E N+L + +C CL+ H HR +QI Sbjct: 2 ESGEFPAWLEVLLKDKFFNACLDHEDDKKNEKNILCIDCC-LTICPHCLSSHTSHRLLQI 60 Query: 401 XSPHTMMLIRXSXIQKVFGLTGVQPTLSTAQGXVF 505 ++R K+ + +QP + + VF Sbjct: 61 RRYVYRDVLRVEDGSKLMDCSLIQPYTTNSSKVVF 95
>gi|9755373|gb|AAF98180.1|AC000107_3 (AC000107) F17F8.4 [Arabidopsis thaliana] Length = 241 Frame 2 hits (HSPs): ___________________ __________________________________________________ Database sequence: | | | | | | 241 0 50 100 150 200 Plus Strand HSPs: Score = 124 (43.7 bits), Expect = 1.2e-06, P = 1.2e-06 Identities = 29/86 (33%), Positives = 47/86 (54%), Frame = +2 Query: 236 PPWLKPLLKESFFVQCKLHADSHKSECNMLLLGLYEWPLCSLCLAHHKDHRAIQIXSPHT 415 P WL+ L+ E+FF C +H KSE N+ L L +C CL H+ H +Q+ Sbjct: 19 PAWLEGLMAETFFSSCGIHETRRKSEKNVFCL-LCCLSVCPHCLPSHRSHPLLQVRRYVY 77 Query: 416 MMLIRXSXIQKVFGLTGVQP-TLSTAQ 493 ++R S ++K+ + VQP T++ A+ Sbjct: 78 HDVVRLSDLEKLIDCSYVQPYTINGAK 104
>gi|5441885|dbj|BAA82383.1| (AP000367) Similar to Arabidopsis thaliana BAC genomic sequence. (AL021889) [Oryza sativa] Length = 306 Frame 2 hits (HSPs): _______ ________ __________________________________________________ Database sequence: | | | | | | | | 306 0 50 100 150 200 250 300 Plus Strand HSPs: Score = 73 (25.7 bits), Expect = 1.4, Sum P(2) = 0.74 Identities = 14/34 (41%), Positives = 17/34 (50%), Frame = +2 Query: 230 RWPPWLKPLLKESFFVQCKLHADSHKSECNMLLL 331 R P WL+ LL FF C H ++ECN L Sbjct: 17 REPAWLRSLLGARFFEACAAHRGMSRNECNQYCL 50 Score = 49 (17.2 bits), Expect = 1.4, Sum P(2) = 0.74 Identities = 12/44 (27%), Positives = 21/44 (47%), Frame = +2 Query: 374 HKDHRAIQIXSPHTMMLIRXSXIQKVFGLTGVQPTLSTAQGXVF 505 H+ HR +Q+ ++R S +++ LT VQ + VF Sbjct: 85 HR-HRVVQVRRSSYHNVVRVSELERTLDLTRVQTYVINRDRVVF 127 Parameters: filter=none matrix=BLOSUM62 V=50 B=50 E=10 gi H=1 sort_by_pvalue echofilter ctxfactor=5.97 Query ----- As Used ----- ----- Computed ---- Frame MatID Matrix name Lambda K H Lambda K H Std. 0 BLOSUM62 0.318 0.135 0.401 +3 0 BLOSUM62 0.318 0.135 0.401 0.339 0.140 0.444 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a +2 0 BLOSUM62 0.318 0.135 0.401 0.325 0.137 0.430 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a +1 0 BLOSUM62 0.318 0.135 0.401 0.352 0.152 0.565 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -1 0 BLOSUM62 0.318 0.135 0.401 0.348 0.151 0.499 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -2 0 BLOSUM62 0.318 0.135 0.401 0.346 0.150 0.525 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -3 0 BLOSUM62 0.318 0.135 0.401 0.350 0.151 0.541 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a Query Frame MatID Length Eff.Length E S W T X E2 S2 +3 0 168 165 10. 75 3 12 22 0.10 34 30 0.10 37 +2 0 169 165 10. 75 3 12 22 0.10 34 30 0.10 37 +1 0 169 165 10. 75 3 12 22 0.10 34 30 0.10 37 -1 0 169 166 10. 75 3 12 22 0.10 34 30 0.10 37 -2 0 169 165 10. 75 3 12 22 0.10 34 30 0.10 37 -3 0 168 165 10. 75 3 12 22 0.10 34 30 0.10 37 Statistics: Database: /usr/local/dot5/sl_home/beauty/seqdb/blast/nr Title: nr Release date: unknown Posted date: 4:06 PM CST Feb 28, 2001 Format: BLAST # of letters in database: 197,782,623 # of sequences in database: 625,274 # of database sequences satisfying E: 11 No. of states in DFA: 593 (58 KB) Total size of DFA: 203 KB (256 KB) Time to generate neighborhood: 0.02u 0.00s 0.02t Elapsed: 00:00:00 No. of threads or processors used: 6 Search cpu time: 163.39u 1.09s 164.48t Elapsed: 00:00:28 Total cpu time: 163.42u 1.11s 164.53t Elapsed: 00:00:28 Start: Mon Oct 1 23:41:44 2001 End: Mon Oct 1 23:42:12 2001
Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000