RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ruvettus(*) pretiosus(*)
FDA Seafood List Common Name: Oilfish(*)
FDA Seafood List Market Name: Oilfish(*)
Unique IEF Gel Code: 19950830gel05
Unique Web Code: oih02Srr
Tissue Aliquot Code: ruvepret H95-002 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	126	14	0.0441571	4	0.294422	16.5	9.2	4.55	126.8	4.2	0.0396532
2	5.2	223	24.9	0.0249014	4	0.162401	9.1	5.1	5.2	222.4	4.2	0.0217584
3	5.85	322	35.9	0.0315035	4	0.190256	10.7	5.9	5.85	321.6	3.6	0.0299987
4	6.55	443	49.4	0.0256171	6	0.261487	14.7	8.2	6.55	442.4	6.1	0.0239989
5	7.35	530	59.1	0.0108889	7	0.113168	6.4	3.5	7.35	530.3	7.1	0.0089585
6	8.15	659	73.5	0.0180765	6	0.160347	9	5	8.15	658.2	6.1	0.0148561
7	8.45	733	81.7	0.0151162	11	0.244334	13.7	7.6	8.45	730.6	10.5	0.0131113
8	8.65	786	87.6	0.0171659	6	0.173075	9.7	5.4	8.65	786.2	6	0.0163339
9	9.3	856	95.4	0.0163394	7	0.180981	10.2	5.7	9.3	854.8	7.3	0.0139065

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.958	40	4.5	0.0363434	5	0.323068	2.5	2.1	3.958	38.8	5.1	0.035647
2	4.418	108	12.1	0.19684	4	1.29925	9.9	8.5	4.418	108.4	3.7	0.198058
3	5.805	318	35.5	0.0156336	4	0.107602	0.8	0.7	5.805	319.1	4.2	0.0144455
4	5.88	330	36.8	0.010437	3	0.054537	0.4	0.4	5.88	328.2	2.9	0.0107301
5	5.945	341	38.1	0.281313	3	1.32499	10.1	8.7	5.945	341.5	2.6	0.285289
6	6.022	354	39.5	0.115792	3	0.666266	5.1	4.4	6.022	354.9	3.4	0.110822
7	6.111	369	41.2	0.120461	3	0.730501	5.6	4.8	6.111	369.7	3.4	0.119631
8	6.164	378	42.2	0.126485	3	0.641175	4.9	4.2	6.164	377.7	3.1	0.116491
9	6.212	386	43.1	0.0290156	7	0.342892	2.6	2.2	6.212	385.5	6.7	0.0288803
10	6.307	402	44.9	0.0638137	2	0.252723	1.9	1.7	6.307	402	2.5	0.0581871
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.366	412	46	0.0305205	7	0.341504	2.6	2.2	6.366	412	6.7	0.0286729
12	6.473	430	48	0.0178857	3	0.0889955	0.7	0.6	6.473	429.4	3	0.0166624
13	6.577	446	49.8	0.0997658	9	1.85363	14.2	12.2	6.577	448.6	8.5	0.122363
14	6.658	455	50.8	0.298811	3	1.31748	10.1	8.6	6.658	456	3.5	0.212939
15	6.767	467	52.1	0.335013	3	1.62626	12.4	10.7	6.767	466.3	2.7	0.338178
16	6.875	479	53.5	0.184382	3	0.88637	6.8	5.8	6.875	479.4	2.6	0.190159
17	7.11	505	56.4	0.0424338	3	0.236099	1.8	1.5	7.11	505.3	3.4	0.0388508
18	7.759	597	66.6	0.0914624	7	0.980103	7.5	6.4	7.759	595.9	6.6	0.0834644

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	129	14.4	0.034149	4	0.221533	14.6	7.9	4.55	129.4	3.7	0.0335239
2	5.2	224	25	0.020346	4	0.128638	8.5	4.6	5.2	223.6	3.9	0.0186703
3	5.85	328	36.6	0.0252336	4	0.148557	9.8	5.3	5.85	328.4	3.5	0.0238137
4	6.55	443	49.5	0.0256727	7	0.314012	20.7	11.2	6.55	441.5	7.5	0.023637
5	7.35	533	59.6	0.0085812	6	0.0694045	4.6	2.5	7.35	532.4	5.7	0.0068354
6	8.15	660	73.7	0.0139509	5	0.13388	8.8	4.8	8.15	659.3	5.4	0.0140776
7	8.45	728	81.3	0.016206	9	0.217686	14.4	7.7	8.45	727.6	8.7	0.0140788
8	8.65	784	87.6	0.014886	7	0.173418	11.5	6.2	8.65	784	7.1	0.0137905
9	9.3	853	95.3	0.0155292	4	0.106587	7	3.8	9.3	852.9	4.2	0.0144616

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.915	49	5.5	0.0067738	7	0.0830115	1.4	1.1	3.915	47.4	7.4	0.0063133
2	4.433	120	13.4	0.0714218	4	0.456167	7.6	6.1	4.433	119.8	3.5	0.0727347
3	4.55	136	15.2	0.023346	4	0.165852	2.8	2.2	4.55	136.6	4.4	0.0214405
4	5.2	225	25.2	0.0142892	4	0.0963658	1.6	1.3	5.2	224.4	4.1	0.0132102
5	5.85	329	36.8	0.0112102	4	0.069964	1.2	0.9	5.85	328.6	3.7	0.0105347
6	5.944	344	38.5	0.0809863	3	0.391197	6.5	5.3	5.944	344.6	2.7	0.0812158
7	6.031	358	40	0.0399109	3	0.227323	3.8	3.1	6.031	358.5	3.4	0.0380356
8	6.137	375	41.9	0.0434351	3	0.244843	4.1	3.3	6.137	374.4	3.3	0.0422702
9	6.188	383	42.8	0.0437145	4	0.265031	4.4	3.6	6.188	382.8	3.7	0.0408512
10	6.238	391	43.7	0.013507	6	0.13749	2.3	1.9	6.238	392.8	6.3	0.0123844
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.344	408	45.6	0.0281065	3	0.124931	2.1	1.7	6.344	408.6	2.7	0.0259625
12	6.412	419	46.9	0.0205152	5	0.164043	2.7	2.2	6.412	419.3	5.4	0.017154
13	6.55	441	49.3	0.0171194	3	0.0765784	1.3	1	6.55	438.4	2.9	0.0148396
14	6.664	455	50.9	0.0378914	13	1.02727	17.1	13.8	6.664	460.7	13.1	0.0443401
15	6.746	465	52	0.151578	3	0.654825	10.9	8.8	6.746	466.1	3.5	0.10604
16	6.836	476	53.2	0.203233	3	0.93095	15.5	12.5	6.836	476.3	2.6	0.202423
17	6.958	491	54.9	0.0796609	3	0.337726	5.6	4.5	6.958	490.8	2.5	0.0754483
18	7.179	518	57.9	0.0152992	4	0.0853079	1.4	1.1	7.179	517.3	3.7	0.0130781
19	7.35	539	60.3	0.0092018	5	0.0663381	1.1	0.9	7.35	537.8	4.8	0.0078604
20	7.76	603	67.4	0.0219429	5	0.198579	3.3	2.7	7.76	602.5	5.1	0.0221266
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	740	82.8	0.0100467	6	0.0908637	1.5	1.2	8.45	737.9	6.5	0.0079182
22	8.65	787	88	0.0079958	4	0.0577032	1	0.8	8.65	785.6	4	0.0081251
23	9.3	855	95.6	0.0071882	5	0.047158	0.8	0.6	9.3	853.2	4.6	0.0057332

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.893	44	4.9	0.0306567	5	0.301674	2.8	2.2	3.893	45.2	5.5	0.0311837
2	4.445	123	13.8	0.178935	4	1.19137	10.9	8.5	4.445	122.9	3.7	0.179575
3	5.771	318	35.6	0.0103876	3	0.0534946	0.5	0.4	5.771	318.2	3	0.0101271
4	5.83	327	36.6	0.0037272	3	0.0194752	0.2	0.1	5.83	328.1	3.2	0.0034806
5	5.916	341	38.1	0.272546	3	1.22422	11.2	8.8	5.916	341.3	2.6	0.263384
6	6.001	355	39.7	0.109384	4	0.67798	6.2	4.9	6.001	354.8	3.9	0.0976068
7	6.097	371	41.5	0.101579	3	0.536992	4.9	3.8	6.097	371.4	3.1	0.0987898
8	6.152	380	42.5	0.111499	3	0.552546	5.1	4	6.152	379.7	3.2	0.0969418
9	6.23	393	44	0.0134788	8	0.25554	2.3	1.8	6.23	387.6	7.7	0.0186648
10	6.309	406	45.4	0.0624511	3	0.270769	2.5	1.9	6.309	406	2.6	0.0581455
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.375	417	46.6	0.0203962	8	0.293143	2.7	2.1	6.375	418	7.8	0.0211236
12	6.502	438	49	0.0187094	3	0.0842116	0.8	0.6	6.502	437.8	2.9	0.0164619
13	6.608	453	50.7	0.0783708	8	1.43472	13.1	10.3	6.608	458.4	7.9	0.102618
14	6.708	465	52	0.253488	2	0.676105	6.2	4.8	6.708	465.4	1.8	0.21179
15	6.807	477	53.4	0.313321	2	1.21082	11.1	8.7	6.807	477.2	2.2	0.314397
16	6.931	492	55	0.19871	3	0.938026	8.6	6.7	6.931	491.6	2.6	0.203508
17	7.147	518	57.9	0.0414601	4	0.255356	2.3	1.8	7.147	518.1	3.5	0.0407104
18	7.771	609	68.1	0.0827742	7	0.954001	8.7	6.8	7.771	607.8	6.7	0.0797864

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	140	15.7	0.0333246	5	0.287762	15.7	9.1	4.55	140.4	5.1	0.0317544
2	5.2	237	26.5	0.0129594	8	0.166403	9.1	5.2	5.2	236.1	7.9	0.0118667
3	5.85	331	37	0.0189537	5	0.162477	8.9	5.1	5.85	331.7	5	0.0184039
4	6.55	451	50.4	0.0277815	8	0.361216	19.7	11.4	6.55	449.2	7.9	0.025657
5	7.35	546	61.1	0.0099216	7	0.105002	5.7	3.3	7.35	544.4	7	0.0084338
6	8.15	674	75.4	0.0191855	7	0.211302	11.5	6.7	8.15	673.9	7.2	0.0166426
7	8.45	737	82.4	0.0185093	9	0.269004	14.7	8.5	8.45	737.4	8.9	0.0170312
8	8.65	795	88.9	0.0154943	5	0.135132	7.4	4.3	8.65	793.2	5.3	0.0144632
9	9.3	859	96.1	0.0145542	6	0.13123	7.2	4.1	9.3	858.3	5.8	0.0128434

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 86

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 16.500 5.200 9.100 5.850 10.700 6.550 14.700 7.350 6.400 8.150 9.000 8.450 13.700 8.650 9.700 9.300 10.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
3.958 2.500 4.418 9.900 5.805 0.800 5.880 0.400 5.945 10.100 6.022 5.100 6.111 5.600 6.164 4.900 6.212 2.600 6.307 1.900 6.366 2.600 6.473 0.700 6.577 14.200 6.658 10.100 6.767 12.400 6.875 6.800 7.110 1.800 7.759 7.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator
4.550 14.600 5.200 8.500 5.850 9.800 6.550 20.700 7.350 4.600 8.150 8.800 8.450 14.400 8.650 11.500 9.300 7.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
3.915 1.400 4.433 7.600 4.550 2.800 5.200 1.600 5.850 1.200 5.944 6.500 6.031 3.800 6.137 4.100 6.188 4.400 6.238 2.300 6.344 2.100 6.412 2.700 6.550 1.300 6.664 17.100 6.746 10.900 6.836 15.500 6.958 5.600 7.179 1.400 7.350 1.100 7.760 3.300 8.450 1.500 8.650 1.000 9.300 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 23 Return to Lane Navigator
3.893 2.800 4.445 10.900 5.771 0.500 5.830 0.200 5.916 11.200 6.001 6.200 6.097 4.900 6.152 5.100 6.230 2.300 6.309 2.500 6.375 2.700 6.502 0.800 6.608 13.100 6.708 6.200 6.807 11.100 6.931 8.600 7.147 2.300 7.771 8.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 18 Return to Lane Navigator
4.550 15.700 5.200 9.100 5.850 8.900 6.550 19.700 7.350 5.700 8.150 11.500 8.450 14.700 8.650 7.400 9.300 7.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Oilfish(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.