RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pollachius virens
FDA Seafood List Common Name: Pollock
FDA Seafood List Market Name: Pollock
Unique IEF Gel Code: 19960118gel02
Unique Web Code: aph03Srr
Tissue Aliquot Code: pollvire A96-002 #05a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	15	2	0.0501532	4	0.365531	8.6	5.3	4.55	14.9	4	0.0516976
2	5.2	114	15	0.0424944	5	0.370394	8.7	5.4	5.2	114.3	5.3	0.0395777
3	5.85	223	29.3	0.0350522	5	0.287942	6.8	4.2	5.85	222.9	4.9	0.0330332
4	6.55	333	43.8	0.0259307	12	0.462671	10.8	6.7	6.55	330.1	11.9	0.0219631
5	7.35	426	56	0.041348	5	0.313607	7.4	4.5	7.35	425.1	4.8	0.0368143
6	8.15	543	71.4	0.0582633	7	0.67512	15.8	9.8	8.15	542.9	7.4	0.051591
7	8.45	607	79.8	0.0608204	6	0.640831	15	9.3	8.45	607.4	6.2	0.0581493
8	8.65	663	87.1	0.0401057	4	0.319803	7.5	4.6	8.65	661.3	3.8	0.0472708
9	9.3	736	96.7	0.0478346	11	0.829062	19.4	12	9.3	734.3	10.9	0.0430833

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.601	24	3.2	0.0937622	8	1.386	10.3	6.9	4.601	24.1	8.4	0.0927579
2	4.743	45	5.9	0.0405896	6	0.434591	3.2	2.2	4.743	48.6	6.4	0.0382306
3	5.122	101	13.3	0.0508852	15	1.30596	9.7	6.5	5.122	94.6	14.6	0.0504242
4	5.249	121	15.9	0.0193842	2	0.0291568	0.2	0.1	5.249	121.3	2	0.0082026
5	5.342	137	18	0.283328	4	1.93614	14.4	9.7	5.342	137.4	4	0.27489
6	5.457	157	20.6	0.13218	4	0.880502	6.5	4.4	5.457	156.9	3.9	0.128216
7	5.642	189	24.8	0.0130933	2	0.0547753	0.4	0.3	5.642	189.4	2.4	0.0126605
8	5.723	203	26.7	0.0193515	3	0.083019	0.6	0.4	5.723	202.6	3	0.0154367
9	5.912	235	30.9	0.01895	3	0.091584	0.7	0.5	5.912	234.3	3.3	0.0155618
10	6.024	253	33.2	0.233867	4	1.47126	10.9	7.4	6.024	253.6	3.6	0.233522
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.124	269	35.3	0.146729	6	1.54116	11.5	7.7	6.124	268.8	6.3	0.138897
12	6.223	285	37.5	0.105046	3	0.581667	4.3	2.9	6.223	284.1	3.1	0.10487
13	6.286	295	38.8	0.027085	3	0.143752	1.1	0.7	6.286	294.7	3	0.0272157
14	6.317	300	39.4	0.0475431	3	0.239747	1.8	1.2	6.317	300.6	2.9	0.0468577
15	6.391	312	41	0.0189986	10	0.497653	3.7	2.5	6.391	321.2	9.7	0.0288375
16	6.478	326	42.8	0.104828	3	0.385579	2.9	1.9	6.478	325.6	2.6	0.0833433
17	6.696	354	46.5	0.0090657	6	0.0791337	0.6	0.4	6.696	353.9	5.7	0.0078057
18	9.043	706	92.8	0.0580797	11	1.11304	8.3	5.6	9.043	710.2	10.6	0.0592298
19	9.205	724	95.1	0.100274	7	1.19608	8.9	6	9.205	724.5	7.5	0.0900733

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	18	2.4	0.0461693	5	0.411345	8.6	5.9	4.55	17.4	5.1	0.0451105
2	5.2	111	14.6	0.0545092	4	0.347697	7.3	5	5.2	111.4	4	0.0493446
3	5.85	227	29.8	0.0273178	6	0.281053	5.9	4	5.85	228.1	6.1	0.0259577
4	6.55	342	44.9	0.0229317	8	0.282666	5.9	4.1	6.55	341.3	7.6	0.0210673
5	7.35	430	56.5	0.0204644	12	0.447894	9.4	6.4	7.35	436.3	12	0.0211462
6	8.15	549	72.1	0.0484311	6	0.474281	9.9	6.8	8.15	548.7	6.2	0.0431821
7	8.45	613	80.6	0.0851866	7	0.957503	20.1	13.8	8.45	611.1	6.7	0.0802676
8	8.65	662	87	0.0509929	5	0.404885	8.5	5.8	8.65	662.2	4.7	0.0486099
9	9.3	733	96.3	0.0970331	7	1.16162	24.4	16.7	9.3	733.4	6.9	0.0945144

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	20	2.6	0.0087896	3	0.04117	0.3	0.3	4.55	19.6	2.7	0.0086169
2	4.633	32	4.2	0.0368319	6	0.444564	3.7	3.4	4.633	33.7	6.5	0.0388632
3	4.744	48	6.3	0.0412717	11	0.732571	6.1	5.5	4.744	49	10.7	0.0387742
4	5.006	86	11.3	0.0188638	10	0.298373	2.5	2.3	5.006	88	9.6	0.017554
5	5.2	114	15	0.0132742	4	0.0908674	0.8	0.7	5.2	114.5	4	0.0128043
6	5.3	132	17.3	0.110953	6	1.20179	10	9.1	5.3	133.4	6.1	0.110451
7	5.406	151	19.8	0.0387781	11	0.597645	5	4.5	5.406	151	10.5	0.0319867
8	5.85	231	30.4	0.0411587	6	0.325661	2.7	2.5	5.85	231.9	6.4	0.028576
9	6.033	260	34.2	0.126668	5	1.1001	9.2	8.3	6.033	260.1	4.8	0.129596
10	6.135	276	36.3	0.100181	6	1.10098	9.2	8.3	6.135	275.3	6.3	0.0990797
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.242	293	38.5	0.059363	4	0.420389	3.5	3.2	6.242	292.5	3.9	0.0611984
12	6.305	303	39.8	0.0072736	4	0.0666911	0.6	0.5	6.305	306.3	3.9	0.0097732
13	6.343	309	40.6	0.0153404	2	0.0423158	0.4	0.3	6.343	309.9	2.2	0.0110184
14	6.419	321	42.2	0.0086231	7	0.120839	1	0.9	6.419	322.5	7	0.0097649
15	6.495	333	43.8	0.0737422	5	0.632672	5.3	4.8	6.495	333.2	5.2	0.0684085
16	7.35	436	57.3	0.0150798	6	0.145137	1.2	1.1	7.35	435	6.4	0.0128684
17	8.15	555	72.9	0.031866	24	1.34518	11.2	10.1	8.15	557	24	0.031623
18	8.45	618	81.2	0.0588809	8	0.672347	5.6	5.1	8.45	617.5	7.7	0.0490936
19	8.65	669	87.9	0.0274044	4	0.180242	1.5	1.4	8.65	669.8	3.8	0.0266719
20	9.206	724	95.1	0.0869303	17	2.45041	20.4	18.5	9.206	720.5	17.3	0.0800241

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.653	35	4.6	0.0738422	12	1.53274	11.3	8.9	4.653	35.1	11.9	0.0725282
2	4.803	57	7.5	0.0422451	9	0.555961	4.1	3.2	4.803	56.3	8.5	0.036733
3	4.981	83	10.9	0.0225611	4	0.159359	1.2	0.9	4.981	83	4.5	0.0200449
4	5.166	110	14.5	0.0438686	10	0.701558	5.2	4.1	5.166	109.1	9.8	0.0405452
5	5.302	133	17.5	0.0152835	4	0.0966362	0.7	0.6	5.302	134.3	3.8	0.0142604
6	5.403	151	19.8	0.224261	5	2.00902	14.8	11.6	5.403	150.9	5.2	0.218556
7	5.511	170	22.3	0.0903045	5	0.793478	5.8	4.6	5.511	169.6	5.4	0.0830872
8	5.697	203	26.7	0.0158937	3	0.0626926	0.5	0.4	5.697	202.5	3.2	0.0110613
9	5.76	214	28.1	0.0078932	4	0.0578852	0.4	0.3	5.76	213.9	4.4	0.0073392
10	6.057	263	34.6	0.20571	4	1.44829	10.7	8.4	6.057	263.5	3.9	0.20844
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.152	278	36.5	0.105894	6	1.14303	8.4	6.6	6.152	278.9	6.3	0.102106
12	6.259	295	38.8	0.107904	3	0.497402	3.7	2.9	6.259	294.5	2.6	0.107211
13	6.353	310	40.7	0.0465859	5	0.321852	2.4	1.9	6.353	309.4	4.6	0.0394693
14	6.422	321	42.2	0.0176384	7	0.163445	1.2	0.9	6.422	321.7	7.2	0.0128803
15	6.498	333	43.8	0.0983194	4	0.628448	4.6	3.6	6.498	333.4	3.9	0.0911145
16	9.082	712	93.6	0.107772	13	2.4785	18.3	14.3	9.082	708.6	12.7	0.110141
17	9.193	723	95	0.115921	6	0.922552	6.8	5.3	9.193	723.5	6.2	0.0842833

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	20	2.6	0.0477264	5	0.396671	9.2	6.3	4.55	20.2	4.8	0.0467431
2	5.2	116	15.2	0.0380124	6	0.356308	8.2	5.7	5.2	117	5.6	0.0361481
3	5.85	229	30.1	0.0271281	8	0.35205	8.1	5.6	5.85	228.7	8.1	0.0245303
4	6.55	341	44.8	0.0270194	9	0.409901	9.5	6.5	6.55	338.8	9.1	0.0253006
5	7.35	436	57.3	0.0295855	6	0.293242	6.8	4.7	7.35	434.9	5.9	0.0278958
6	8.15	559	73.5	0.0513606	7	0.575803	13.3	9.2	8.15	557.5	6.7	0.0481556
7	8.45	621	81.6	0.0767681	7	0.951921	22	15.2	8.45	618.7	7	0.0769995
8	8.65	669	87.9	0.0574089	4	0.446016	10.3	7.1	8.65	668.8	4.2	0.0593128
9	9.3	734	96.5	0.0387573	9	0.548827	12.7	8.8	9.3	735	8.5	0.0363164

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 83

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.600 5.200 8.700 5.850 6.800 6.550 10.800 7.350 7.400 8.150 15.800 8.450 15.000 8.650 7.500 9.300 19.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.601 10.300 4.743 3.200 5.122 9.700 5.249 0.200 5.342 14.400 5.457 6.500 5.642 0.400 5.723 0.600 5.912 0.700 6.024 10.900 6.124 11.500 6.223 4.300 6.286 1.100 6.317 1.800 6.391 3.700 6.478 2.900 6.696 0.600 9.043 8.300 9.205 8.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 19 Return to Lane Navigator
4.550 8.600 5.200 7.300 5.850 5.900 6.550 5.900 7.350 9.400 8.150 9.900 8.450 20.100 8.650 8.500 9.300 24.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 0.300 4.633 3.700 4.744 6.100 5.006 2.500 5.200 0.800 5.300 10.000 5.406 5.000 5.850 2.700 6.033 9.200 6.135 9.200 6.242 3.500 6.305 0.600 6.343 0.400 6.419 1.000 6.495 5.300 7.350 1.200 8.150 11.200 8.450 5.600 8.650 1.500 9.206 20.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 20 Return to Lane Navigator
4.653 11.300 4.803 4.100 4.981 1.200 5.166 5.200 5.302 0.700 5.403 14.800 5.511 5.800 5.697 0.500 5.760 0.400 6.057 10.700 6.152 8.400 6.259 3.700 6.353 2.400 6.422 1.200 6.498 4.600 9.082 18.300 9.193 6.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 17 Return to Lane Navigator
4.550 9.200 5.200 8.200 5.850 8.100 6.550 9.500 7.350 6.800 8.150 13.300 8.450 22.000 8.650 10.300 9.300 12.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.