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Research Project:
Indian Ricegrass and Needle and Thread Grass Genetic Diversity Assessment in the Grand Staircase Escalante National Monument
Location: Forage and Range Research
2008 Annual Report
1a.Objectives (from AD-416)
Characterize the genetic diversity, geographic provenance, population structure and geographic and genetic correlations of natural populations of Indian Ricegrass and Needle and Thread Grass from the Grand Staircase Escalante National Monument and other locations in the western United States.
1b.Approach (from AD-416)
Genetic studies of wildland collections of Indian Ricegrass and Needle and Thread Grass from within the Grand Staircase Escalante National Monument (12-15 accessions), from adjacent areas (10-12 accessions), and from distant collections throughout the Great Basin (20-22 accessions), as well as two developed releases of Indian Ricegrass will be conducted. Analysis of Amplified Fragment Length Polymorphism (AFLP) will be used to estimate the genetic variation within and between accessions of each of these species. AFLP profiles for a total of 384 individual plants (8 plants from each of 48 accessions) will be generated using 4-6 selective amplification primer pairs for each species. Geographic provenance will be characterized with ArcGIS v9.1, genetic diversity assessed with Leonard's similarity index, population structure assessed with Analysis of Molecular Variance (AMOVA) and diversity estimated as per Excoffier et al., geographic and genetic correlations will be characterized with Mantel's Test using NTSYS-pc, and dendrogram construction with PAUP.
3.Progress Report
Seed from the Grand Staircase Escalante National Monument was collected by the BLM biologist during June, 2008 and was shipped to the FRRL at that time. Work will begin work in September, 2008 to clean and germinate the seed which was collected. DNA extraction and AFLP profiling will begin immediately after plant tissue is available. Monitoring mmethods used include emials, conference calls and written reports.
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Last Modified: 03/16/2009
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