Effect of the Fluorescein to Protein Ratio on Anti-Treponema pallidum Conjugates

Appl Microbiol. 1969 November; 18(5): 806–809.

PMCID: PMC378092

Effect of the Fluorescein to Protein Ratio on Anti-Treponema pallidum Conjugates

Susan M. Mothershed

Venereal Disease Research Laboratory, Venereal Disease Branch, State and Community Services Division, National Communicable Disease Center, Atlanta, Georgia 30333

Abstract

The effect of using different labeling techniques and various amounts of fluorescein-isothiocyanate was tested with several pools of antisera to Treponema pallidum. Optimal labeling occurred in all serum pools at a fluorescien to protein ratio of 15. The use of pure dye and the dialysis method of labeling is recommended as the method of choice because of its effectiveness in the reduction of nonspecific staining.

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Selected References

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