Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Blue Crab  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Callinectes sapidus
FDA Seafood List Common Name: Blue Crab
FDA Seafood List Market Name: Crab, Blue
Unique IEF Gel Code: 19970218gel02
Unique Web Code: cbh02Srr
Tissue Aliquot Code: callsapi K96-013 Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55202.60.080452540.5003844.83.54.55203.60.0773997
25.210914.20.12115140.8622048.26.15.2109.84.30.113689
35.8522128.70.25844931.4512913.810.25.85220.73.20.255298
46.5536647.50.12829481.6161315.411.46.55364.17.80.116998
56.8539751.60.097988240.6472216.24.66.85397.640.0920647
67.3546460.30.16744931.003159.67.17.35463.63.40.168181
78.15585760.12788161.3546612.99.58.15585.36.30.121287
88.4565284.70.17338161.784271712.68.45651.460.168986
98.6570691.70.12069640.769387.35.48.65705.53.70.117671
109.375898.40.11766120.5127924.93.69.3757.62.40.122085
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.554202.60.030816240.2307951.50.94.55419.74.30.0300889
24.639324.20.038196630.2057761.30.84.63932.42.90.0395639
34.746476.10.064147840.4362232.71.74.74646.73.90.063555
44.854628.10.020584440.15811210.64.85462.14.50.0200389
54.911709.10.11648220.461472.91.84.91170.22.20.116124
65.0619111.80.078215491.21017.64.75.06192.59.30.0731867
75.182108140.057615110.943695.93.75.182108.910.90.0488836
85.33513317.30.17272661.90215127.45.335134.45.90.181289
95.38914218.40.44485753.5506922.413.85.3891434.80.415602
105.50316120.90.006319610.01748250.10.15.503161.51.50.0066187
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.5817422.60.075129930.3637892.31.45.581742.70.075699
125.65218624.20.041897930.1919141.20.75.652186.52.60.0422659
135.72419825.70.027097520.1068340.70.45.724198.32.20.0276886
145.75420326.40.032818520.115680.70.45.754202.820.0327953
155.80221127.40.015495220.05185180.30.25.802211.21.80.015922
165.97124431.70.13422651.007166.33.95.971243.24.80.118429
176.0125232.70.14450130.6842534.32.76.01252.62.80.140277
186.058262340.076280120.2824271.81.16.058261.92.20.0712593
196.17528637.10.12188661.1109874.36.175285.46.30.0988386
206.20829338.10.16294320.6225973.92.46.208293.52.30.150402
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.26230439.50.12305240.9960636.33.96.262302.74.30.129714
226.3331841.30.1121230.5842943.72.36.33317.42.90.114536
236.38332942.70.038210930.2197141.40.96.383328.830.0410163
246.75838650.10.034127270.4088182.61.66.758388.57.20.0320602
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55182.30.054816340.3330194.43.34.5518.63.50.0531051
25.211114.40.10256340.7368429.77.45.21114.20.0990174
35.8521628.10.22993331.14031511.45.85216.32.80.232267
46.5536046.80.083031191.213215.912.16.55357.29.30.0733269
56.8539451.20.058948540.3670984.83.76.85393.83.70.0553809
67.3546360.20.096117540.6236158.26.27.35462.53.60.0967481
78.1558475.90.087165660.94058512.49.48.15583.46.10.087359
88.4565284.80.11304961.1603315.211.68.45651.45.90.11153
98.6570591.70.088469230.5373387.15.48.65704.33.50.0878604
109.3754980.12050930.5565047.35.69.3753.72.50.12469
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.591253.20.030449120.1152490.80.54.59125.320.0324151
24.642334.30.013572630.08504970.60.34.64234.23.40.0141054
34.7254660.038272230.2307291.70.94.72546.23.40.0379827
44.86678.70.06837420.2661421.914.86672.10.0699006
54.9958811.40.023309750.2198011.60.94.99588.75.30.0232195
65.0599812.70.028786150.2226431.60.95.05997.14.90.0258105
75.212015.60.059610650.484333.51.95.2120.24.90.0554264
85.269131170.17495231.020567.445.269130.63.30.175726
95.33314118.30.42570953.8004827.5155.333141.84.90.437243
105.465162210.007511320.03235560.20.15.465161.42.50.0073391
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.54117422.60.049923730.2542991.815.541174.42.90.0497643
125.62318724.30.014822930.07298250.50.35.623186.92.60.0155973
135.71720226.20.01948140.1226660.90.55.717200.540.017438
145.85223290.15815630.7542945.535.85223.12.70.159962
155.95124331.60.10247140.5613584.12.25.951242.83.60.0869519
165.99625232.70.050917120.1589721.20.65.996252.51.70.0518405
176.046262340.014155820.04654750.30.26.046262.71.50.0174151
186.17228737.30.053541740.3287272.41.36.172286.23.80.0491574
196.20829438.20.083449920.3556532.61.46.208293.82.40.0820541
206.25830439.50.057964540.4469333.21.86.258302.84.30.0587955
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.32331741.20.052204830.2459711.816.323316.82.60.0525272
226.55362470.0560675100.8902956.43.56.55359.79.90.0509611
236.8539250.90.044130880.4872663.51.96.85394.47.70.0358715
247.3546760.60.060777440.4387253.21.77.35465.94.10.0601375
258.1558776.20.038664770.4681123.41.88.15587.47.30.0359954
268.4565585.10.094923871.137678.24.58.456546.90.0936866
278.6570791.80.04338630.2387881.70.98.6570730.0445745
289.375598.10.065994430.3286382.41.39.3754.72.70.068886
PeakCounter: 28
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.528151.90.013730630.06563140.50.34.52814.52.60.0142394
24.615283.60.052146740.2954942.41.24.61529.13.90.0426954
34.702415.30.092139991.36883115.44.70240.79.50.0816793
44.822597.70.10783330.5325864.32.14.82258.53.10.0982253
54.9698110.50.04601290.7699846.234.96982.39.40.0461387
65.0569412.20.01762630.06467360.50.35.056962.80.0132543
75.246123160.14346741.083078.74.35.246122.24.20.147006
85.3213517.50.36232163.9404631.715.65.32134.85.90.379285
95.45415720.40.006682530.02595420.20.15.454157.92.50.0058212
105.5417122.20.039871830.2191721.80.95.54171.63.20.0390555
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.626185240.020766920.07439510.60.35.626184.820.020682
125.72420126.10.021384360.2022791.60.85.724199.76.50.0175954
135.91223430.40.058430130.307672.51.25.912234.82.90.0599479
145.94224031.20.11944530.5830044.72.35.942240.52.90.112863
155.98624932.30.083910920.3009562.41.25.986249.32.10.0799991
166.03625933.60.023705920.07062620.60.36.036259.21.70.0235188
176.14928236.60.062597440.3932123.21.66.149281.43.80.0587025
186.17928837.40.099460320.4318223.51.76.179288.52.50.0979953
196.23329938.80.080540450.6282545.12.56.2332984.70.0759282
206.29831240.50.072745330.3710831.56.298312.32.90.0724896
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.36732642.30.017458870.1931431.60.86.3673296.70.0162699
226.60836947.90.021473650.1739721.40.76.608369.74.60.0211181
236.80238950.50.060156630.3263782.61.36.8023893.10.0585212
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55182.30.075206440.448755.244.5518.13.50.0713315
25.211114.40.10116850.8352939.77.55.2111.54.90.0967733
35.8522028.60.26150431.2530814.611.25.852202.70.261032
46.5536447.30.090973571.0730312.59.66.553627.30.0829833
56.8539651.40.069738640.4382755.13.96.85396.53.80.0657247
67.3546460.30.11717440.7295148.56.57.35464.23.50.116505
78.1558676.10.1019661.1336913.210.18.15585.86.30.101433
88.4565484.90.15061561.5028317.513.48.45653.55.80.146339
98.6570791.80.10064540.6088027.15.48.65706.53.50.0971036
109.375698.20.13292820.5657496.65.19.3755.52.30.136478
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 105
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 28 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln