Evaluation of Herbaceous Biomass Crops in the Northern Great Plains: Results and Discussion: Part 2

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Chemical Composition of Perennial Species

Chemical composition of perennial biomass species as influenced by environments is presented in Table 4. Many chemical components had a significant year X site interaction indicating that the chemical composition can be expected to vary with site and production year. The significant interaction was caused by changes in relative ranking of a component among years. For example, the ash concentration was highest at Glenfield Good in 1989, 1991, and 1992, but lowest in 1990. The data suggest that it is impossible to accurately predict the chemical composition of a perennial biomass species at a particular site and year by other than a "ballpark" figure.

Nitrogen fertilization had little effect on the chemical composition of perennial biomass species in the first year of production (Table 5), and had several significant effects at only Glenfield Good and Carrington irrigated in 1990. Significant differences by N level were detected in about half of the comparisons of chemical components within sites in 1991 and 1992, but a consistent trend for N level effects across sites was evident only for N concentration (data not shown). Where significant N effects were detected, ash, ADF, TNC, and cellulose concentrations generally decreased with increasing N level and NDF, ADL, N, and hemicellulose increased. Even where significant differences in chemical composition occurred, relative differences were small for all but N concentration, indicating that N fertilization has its greatest effect on biomass yield and only minor effects on the chemical composition. Nitrogen effects within 1991 and 1992 were discussed by Meyer et al. (1993).Cool-season grass species/mixtures at Prosper generally differed little in chemical composition when meaned across years and N levels, the "ballpark" estimate (Table 6, Fig. 3). This is not too surprising when the makeup of field plots was evaluated. The three cool-season mixtures generally had few legume components and/or were dominated by intermediate wheatgrass. This caused bromegrass and brome-alfalfa, and intermediate wheatgrass, intermediate/western wheatgrasses, and the CRP

Figure 1. Part 1

Figure 3. Part 2

mixture to be quite similar in chemical composition and act more like two treatments than five. Still, reed canarygrass, bromegrass, and brome-alfalfa generally had higher ash and N concentrations than the other species/mixtures, but NDF, ADF, hemicellulose, and cellulose concentrations were very similar. Crested wheatgrass had a higher TNC and a lower ash content than most other cool-season species/mixtures. Bromegrass and brome-alfalfa were slightly higher in ADL.

Switchgrass and big bluestem [only isolated comparisons, Meyer et al. (1993)], warm-season species, had significantly greater NDF and hemicellulose and less ash, N, and ADL concentrations than most cool-season species/mixtures (Fig. 3, Table 6). These species were the major cause of significant species differences in chemical composition where included in the treatments (especially at Prosper). These differences were expected and follow known differences between warm- and cool-season grasses.

Table 4. Chemical composition of perennial biomass species as influenced by site and year (meaned across species and N levels).

Chemical component†
Site Ash NDF ADF ADL N TNC HEMI CELL
-----------------------------g kg-1 dry weight-------------------------
1989
Prosper 90 698 438 62 17.0 58 260 374
Glenfield Good 156 613 375 53 22.0 52 238 322
Leonard 115 684 453 57 17.0 93 231 395
Glenfield Poor 144 627 363 45 21.0 68 264 318
Carrington (irrigated) 89 638 437 68 10.0 -- 201 374
1990
Prosper 108 683 455 63 17.0 73 228 392
Hettinger 110 659 399 48 18.0 69 260 351
Glenfield Good 81 667 430 51 11.0 107 237 379
Leonard H1‡ 109 685 449 58 16.0 88 236 391
Leonard H2‡ 111 653 341 37 23.0 -- 312 304
Glenfield Poor 80 658 419 50 12.0 67 239 369
Carrington (irrigated) 84 639 434 55 13.0 111 205 379
1991
Prosper 100 662 412 56 15.0 75 250 356
Hettinger 108 649 381 48 18.1 82 268 333
Glenfield Good 144 658 403 48 13.0 93 255 354
Leonard 84 686 440 57 10.6 82 246 384
Glenfield Poor 109 664 402 49 12.7 79 262 353
Carrington (irrigated) 95 641 433 53 9.4 92 208 380
1992
Prosper 90 629 389 43 16.2 71 240 346
Glenfield Good 115 618 384 40 14.9 50 234 343
Leonard 90 658 409 44 11.4 72 250 365
Glenfield Poor 103 615 385 39 15.0 66 231 346
Carrington (irrigated) 98 607 380 40 11.3 90 219 348


† NDF=neutral-detergent fiber, ADF=acid-detergent fiber, ADL=acid-detergent lignin, N=nitrogen, TNC=total nonstructural carbohydrates, HEMI=hemicellulose (NDF-ADF), and CELL=cellulose (ADF-ADL).
‡ H1=Harvest 1, H2=Harvest 2.

 

Table 5.Significance of nitrogen effect on chemical composition of perennial biomass species at six North Dakota sites in 1989-92 (meaned across species).

Chemical component
Site Ash NDF ADF ADL N TNC HEMI CELL
1989
Prosper * NS NS NS NS NS NS NS
Glenfield Good NS NS NS NS NS NS NS NS
Leonard NS NS NS NS NS NS NS NS
Glenfield Poor NS NS NS NS NS * NS NS
Carrington irrigated NS NS NS NS NS -- NS NS
1990
Prosper NS NS NS NS NS NS NS NS
Hettinger NS NS NS NS NS NS NS NS
Glenfield Good NS * NS * * * * *
Leonard NS NS NS NS NS * NS NS
Glenfield Poor NS NS NS NS * NS NS *
Carrington irrigated NS NS * * * NS NS *
1991
Prosper NS NS NS * * * NS *
Hettinger NS * * * * NS NS *
Glenfield Good * NS * NS * NS * *
Leonard NS NS NS NS NS NS NS NS
Glenfield Poor * NS * NS * * * *
Carrington irrigated * * NS NS * NS NS NS
1992
Prosper NS NS * * * NS NS *
Glenfield Good * NS * NS * NS * *
Leonard NS NS NS * * NS NS NS
Glenfield Poor * NS NS NS * NS NS NS
Carrington irrigated * * NS NS * NS * NS


* Significant at P< 0.05.
NDF=neutral-detergent fiber, ADF=acid-detergent fiber, ADL=acid-detergent lignin, N=nitrogen, TNC=total nonstructural carbohydrates, HEMI=hemicellulose (NDF-ADF), and CELL=cellulose (ADF-ADL).

Cool-season grass species/mixtures at sites other than Prosper also differed little in chemical composition when meaned across years and N levels (Table 6). Crested wheatgrass generally was the lowest species/mixtures in ash, cellulose, and ADF concentrations across sites. Bromegrass and brome- alfalfa generally were highest of the cool-season species/mixtures in ash and N concentrations.

Table 6.Chemical composition of several perennial biomass species at six North Dakota sites (meaned across years and 3 or 4 N levels).

Chemical component†
Species Ash NDF ADF ADL N TNC HEMI CELL
---------------------------g kg-1 dry weight-----------------------
Prosper (Site 1)
Bromegrass 100 659 423 60 16.7 63 236 363
Intermediate wheatgrass(I) 92 665 426 53 12.7 69 239 373
Crested wheatgrass 80 654 405 60 16.1 81 250 345
I & western wheatgrass 98 671 429 52 15.4 62 242 377
CRP mixture 92 665 427 53 15.6 69 238 374
Brome-alfalfa 105 655 420 64 19.2 59 235 357
Switchgrass‡ 70 723 413 47 10.0 82 310 366
Reed canarygrass‡ 130 662 416 51 20.0 67 246 365
Hettinger (Site 2)
Bromegrass 121 660 399 47 20.4 54 261 352
Intermediate wheatgrass 86 663 385 51 16.1 98 278 334
Crested wheatgrass 126 632 386 49 21.9 61 246 337
Brome-alfalfa 103 650 388 46 17.1 79 262 341
Glenfield Good (Site 3)
Bromegrass 131 643 402 48 17.2 60 241 355
Intermediate wheatgrass 120 636 397 48 14.4 83 239 350
Crested wheatgrass 95 639 398 54 12.1 94 242 344
I & western wheatgrass 128 644 391 44 14.6 76 253 348
CRP mixture 129 632 400 47 14.5 73 232 353
Brome-alfalfa 143 636 398 48 18.0 63 239 350
Leonard (Site 4)
Bromegrass‡ 89 661 425 54 12.8 87 236 371
Intermediate wheatgrass 108 659 428 48 14.6 83 232 379
Crested wheatgrass 94 661 425 58 13.9 97 236 367
I & western wheatgrass 101 670 429 48 13.8 86 241 380
CRP mixture 106 686 453 54 14.1 70 233 399
Brome-alfalfa 107 666 438 57 15.5 88 229 380
Switchgrass‡ 76 738 455 59 8.7 92 283 395
Reed canarygrass‡ 105 668 433 48 13.8 63 235 386
Glenfield Poor (Site 5)
Bromegrass 122 643 395 46 17.2 55 248 349
Intermediate wheatgrass 101 638 395 44 14.0 70 243 352
Crested wheatgrass 95 626 382 52 13.6 85 245 330
I & western wheatgrass 113 642 385 41 15.1 78 258 344
CRP mixture 108 632 390 44 15.2 72 241 347
Brome-alfalfa 110 665 405 49 15.8 55 260 356
Carrington irrigated (Site 6)
Bromegrass 95 637 427 51 10.8 99 210 376
Intermediate wheatgrass 88 627 417 49 10.5 100 209 368
Crested wheatgrass 74 641 405 56 10.2 103 236 349
I & western wheatgrass 91 631 425 49 9.6 99 206 377
CRP mixture 94 627 437 61 12.6 96 190 376
Brome-alfalfa 101 625 423 55 11.9 94 202 368


† NDF=neutral-detergent fiber, ADF=acid-detergent fiber, ADL=acid-detergent lignin, N=nitrogen, TNC=total nonstructural carbohydrates, HEMI=hemicellulose (NDF-ADF), and CELL=cellulose (ADF-ADL).
‡ Mean has only three years included.

Species differences in chemical composition within years and sites frequently were detected (Table 7) in contrast to when meaned across years (Table 6). These differences have been discussed previously by Meyer et al. (1990, 1992, 1993).

The yield of chemical components (biomass yield times concentration of each component) of perennial biomass species at six sites in 1989-92 is presented in Table 8. The absolute yields were very variable among sites and years. For example, yields of NDF ranged form 0.70 to 6.29 Mg ha-1 across years and sites, or a 9-fold difference. Likewise, TNC ranged from 0.08 to 1.07 Mg ha-1 and ADL ranged from 0.5 to 0.59 Mg ha-1. Most chemical component yields were primarily dependent on biomass yields; although, the small differences detected in chemical composition among species/mixtures did at times contribute to yield differences (data not presented).

Corn Grain Yields

Corn for grain was included in these experiments as an environmental check. Grain yield ranged from 9.2 to 10.9 Mg ha-1 at the Carrington irrigated site across the 3 test years (Table 9), about that expected at this site. Grain yields at the dryland sites were much more variable. For example, grain yields at Prosper ranged from 2.0 Mg ha-1 in the 1988 dry year to 11.0 Mg ha-1 on fallow in 1990. Leonard had a very similar range to that of Prosper, but grain yields were significantly lower in 1991 and 1992 at Leonard than at Prosper. Grain yields at Glenfield were at best disappointing. Grain did not set at Hettinger in 1988, 1990, and 1991 due to droughty conditions (Table 1). Grain yields were not obtained at Glenfield Good and Poor due to an undetected zinc deficiency in 1988 and 1989.

Nitrogen level and cropping system (fallow vs. wheat) management intensities rarely were significant (Table 9), in part due to the few degrees of freedom to test the cropping system effects, but also due to variable yields. Prosper in 1988 and Leonard in 1991 were the only exceptions where significance was detected.

Biomass Yield of Annuals

Biomass yield of annual herbaceous crops as affected by cropping system at two sites is presented in Table 10. Biomass yields averaged 1 to 1.2 Mg ha-1 greater on fallow than on recrop wheat land at the two sites. Biomass yields were not significantly affected by the cropping system in 1991 and 1992 at both sites, but were greater on fallow than on recropped wheat land in 1990 at Leonard. Biomass yields were unaffected by the cropping system at Glenfield Poor in 1991, but were higher from recrop than from fallow at Glenfield Good in 1991 (Table 11). The reason for this last response is unclear.

Nitrogen levels above 50 kg N ha-1, the lowest level used, generally did not affect the biomass yield of annual herbaceous crops at most sites or years (Table 12). Likewise, the N level X species interaction generally was nonsignificant; therefore, species results are presented at 50 kg N ha-1.

Biomass yields meaned across years of annual species at 50 kg N ha-1 as affected by site and cropping system is presented in Figure 4. Maximum biomass yield (16.9 Mg ha-1) was obtained with forage sorghum at Carrington during 1988-91. Likewise, forage sorghum has been the highest yielding annual species on dryland averaging 16.2 Mg ha-1 at Prosper on fallow during 1988-92 and the highest yielding species on recrop or fallow at Prosper, Leonard, and Glenfield Good. Sorghum X sudan has been only slightly lower yielding than forage sorghum at the high-moisture sites and higher yielding at Hettinger and Glenfield Poor, the droughty sites. This result follows what we anticipated when these experiments were planned. Foxtail millet generally has been the lowest yielding species at most sites, but may have a place where a short-season crop is needed.

Table 7. Significance of species on chemical composition of perennial biomass species at six North Dakota sites in 1989-92.

Chemical component†
Site Ash NDF ADF ADL N TNC HEMI CELL
1989
Prosper * * NS * * NS NS NS
Glenfield Good * * * * * * NS *
Leonard NS NS * NS NS NS NS *
Glenfield Poor NS NS NS * * NS NS NS
Carrington irrigated * * * * * -- * *
1990
Prosper * * * * * * NS *
Hettinger NS NS * NS * * * NS
Glenfield Good * NS * * * * NS *
Leonard NS * NS * NS NS NS NS
Glenfield Poor * NS NS * NS NS NS NS
Carrington irrigated * * NS * NS * NS NS
1991
Prosper * * * * * * * *
Hettinger * * * * * * * *
Glenfield Good NS * NS NS * NS NS NS
Leonard * * NS NS NS * * *
Glenfield Poor NS * NS * NS NS NS NS
Carrington irrigated * NS * * NS NS NS *
1992
Prosper * * * * * NS NS *
Glenfield Good NS * NS * * NS * NS
Leonard * * * NS NS * NS *
Glenfield Poor NS NS NS NS NS * * NS
Carrington irrigated * * * * NS NS NS *


† NDF=neutral-detergent fiber, ADF=acid-detergent fiber, ADL=acid-detergent lignin, N=nitrogen, TNC=total nonstructural carbohydrates, HEMI=hemicellulose (NDF-ADF), and CELL=cellulose (ADF-ADL).

Kochia has been the most surprising species. Kochia and sweetclover were initially included as a volunteering species, but it became clear that kochia has allelopathic compound(s), which are autotoxic to the kochia plant. Biomass yield of volunteering kochia was less than half that of annually seeded kochia (data not presented). Therefore, volunteering kochia was deleted and annually seeded kochia included instead. In addition, sweetclover was not volunteering adequately so this treatment was discontinued also.

Table 8. Yield of eight chemical components of perennial biomass species at six North Dakota sites in 1989-1992 (meaned across species and N levels).

Chemical component†
Site Ash NDF ADF ADL N TNC HEMI CELL
---------------------------------Mg ha-1-----------------------------
1989
Prosper 0.60 4.85 3.07 0.43 0.120 0.50 1.78 2.64
Glenfield Good 0.30 1.22 0.74 0.10 0.043 0.10 0.47 0.64
Leonard 1.03 6.13 4.06 0.52 0.155 0.83 2.07 3.54
Glenfield Poor 0.15 0.70 0.41 0.05 0.022 0.08 0.29 0.36
Carrington (irrigated) 0.79 5.72 3.92 0.59 0.090 --- 1.80 3.33
1990
Prosper 0.75 4.74 3.15 0.43 0.12 0.51 1.59 2.72
Hettinger 0.28 1.70 1.03 0.12 0.04 0.19 0.67 0.91
Glenfield Good 0.51 4.31 2.78 0.33 0.07 0.67 1.53 2.44
Leonard 1.01 6.29 4.15 0.54 0.15 1.02 2.14 3.61
Glenfield Poor 0.40 3.37 2.14 0.25 0.06 0.34 1.23 1.88
Carrington (irrigated) 0.80 6.10 4.14 0.52 0.12 1.07 1.96 3.61
1991
Prosper 0.66 4.50 2.80 0.38 0.095 0.51 1.70 2.41
Glenfield Good 0.44 2.05 1.25 0.15 0.040 0.29 0.80 1.10
Leonard 0.53 4.37 2.81 0.38 0.067 0.53 1.56 2.45
Glenfield Poor 0.31 1.92 1.16 0.14 0.037 0.23 0.76 1.02
Carrington (irrigated) 0.55 3.72 2.52 0.31 0.054 0.53 1.20 2.21
1992
Prosper 0.55 3.98 2.46 0.27 0.101 0.43 1.52 2.18
Glenfield Good 0.29 1.64 1.02 0.11 0.040 0.14 0.63 0.91
Leonard 0.64 4.85 3.03 0.32 0.083 0.50 1.82 2.70
Glenfield Poor 0.22 1.37 0.86 0.09 0.034 0.15 0.51 0.77
Carrington (irrigated) 0.48 3.03 1.94 0.20 0.058 0.44 1.09 1.74


† NDF=neutral-detergent fiber, ADF=acid-detergent fiber, ADL=acid-detergent lignin, N=nitrogen, TNC=total nonstructural carbohydrates, HEMI=hemicellulose (NDF-ADF), and CELL=cellulose (ADF-ADL).

Biomass yields of annually seeded kochia have been nearly equal to forage sorghum at Prosper and the highest yielding species at Hettinger (Fig. 4, Table 11). As a weedy species commonly found in the northern Great Plains, this species appears well adapted to droughty environments. However, it did not perform well at Leonard; although, biomass yields reported at many sites may have been reduced by inappropriate harvest dates. See the section on maturity effects latter in this report. In addition, poor stand establishment in 1991 resulted in either no observation or reduced yields from the low plant density. It is clear that additional work is needed on use of kochia as an annually seeded biomass species.

Table 9. Grain yield of corn by N level and cropping system at six North Dakota sites in 1988-92.

Cropping Nitrogen level (kg ha-1)
Site System 50 100 200 LSD (0.05) CV
-----Mg ha-1 @ 155 g kg-1 moisture----- --%--
1988
Prosper Recrop 2.0 4.2 3.9 0.9 11.2
Leonard Fallow 3.0 2.7 2.8 NS 14.3
Carrington Irrigated 10.2 9.9 10.3 NS 12.2
1989
Prosper Wheat 5.9 4.4 4.3 NS 20.8
Fallow 5.9 5.6 5.3
Hettinger Wheat 6.8 7.9 6.7 NS 71.2
Fallow 4.1 3.0 5.1
Leonard Wheat 3.8 3.9 4.7 NS 16.9
Fallow 5.1 4.2 5.2
1990
Prosper Wheat 10.4 9.6 9.9 NS 11.7
Fallow 10.2 11.0 11.0
Glenfield Good Fallow 9.3 9.0 9.6 NS 9.1
Leonard Wheat 7.8 7.5 7.6 NS 9.9
Fallow 9.3 10.8 9.4
Glenfield Poor Fallow 5.9 5.4 6.4 NS 11.8
Carrington Irrigated 10.9 9.2 9.5 NS 21.1
1991
Prosper Wheat 7.7 8.7 8.6 NS 15.7
Fallow 7.9 8.7 8.6
Glenfield Good Wheat 3.6 3.5 4.2 NS 23.7
Fallow 4.3 3.6 3.5
Leonard Wheat 4.8 5.3 4.6 2.8 18.6
Fallow 4.8 6.4 7.4
Glenfield Poor Wheat 3.7 3.6 3.7 NS 20.3
Fallow 2.6 2.6 2.4
Carrington Irrigated 9.7 10.6 10.3 NS 5.8
1992
Prosper Wheat 8.1 7.7 9.8 NS 9.4
Fallow 7.4 9.4 8.4
Glenfield Good Wheat 2.8 2.8 2.8 NS 21.2
Leonard Wheat 5.5 5.5 5.1 NS 14.8
Fallow 5.1 5.4 5.5

 

Table 10. Biomass yield of annual herbaceous crops as affected by cropping system at two sites in North Dakota (mean of species and N levels).

Cropping system
Site Year Fallow Wheat LSD (0.05)
-------------Mg ha-1-------------
Prosper 1989 12.0 10.6 *
1990 19.8 17.6 *
1991 15.3 15.1 NS
1992 14.4 13.5 NS
Mean 15.4 14.2
Leonard 1989 7.8 8.4 NS
1990 16.1 12.1 *
1991 14.5 12.8 NS
1992 6.9 7.8 NS
Mean 11.3 10.3

The highest biomass yield recorded was 23.3 Mg ha-1 for forage sorghum grown at Prosper on fallow in 1990 (Table 11). Species effects within years have been discussed previously (Meyer et al., 1989-93).