Idziorek T, Klatzmann D; International Conference on AIDS.

Int Conf AIDS. 1991 Jun 16-21; 7: 103 (abstract no. W.A.1044).

Hopital de la Pitie, Paris, France

OBJECTIVE: To construct and analyze the anti-HIV properties of CD4-coated red blood cells. METHODS: We have previously described an efficient chemical method to cross-link soluble CD4 to red blood cells (Biochem. Biophys. Acta, 1991, 1062, p 39-45). Here we have analyzed the anti-HIV properties of CD4 red blood cells (CD4-RBC) using classical binding and infectivity assays. RESULTS: We demonstrate that CD4-RBC, but not intact RBC, specifically inhibit in a dose-dependent manner the binding and infection by HIV of CD4+ target cells. DISCUSSION AND CONCLUSIONS: On a CD4-molar basis, this inhibition is approximately 10 times more efficient with CD4-RBC than with soluble CD4. These results suggest that CD4-RBC might be a promising anti-HIV therapeutic agent provided they retain the long life span of RBC.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Anti-HIV Agents
• Antigens, CD4
• Erythrocytes
• HIV
• HIV Core Protein p24
• HIV Envelope Protein gp120
• HIV Infections
• HIV Seropositivity
• Recombinant Proteins
• SK&F 106528
• immunology

Other ID:

• 3104491

UI: 102192236

From Meeting Abstracts