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Library:10272 (dbEST ID)
Zea mays sperm cell cDNA library
Library Description
| Organism: | Zea mays |
| Cultivar: | A188 |
| Cell type: | sperm |
| Vector: | Clontech Triplex2 |
RNA was isolated from fluorescent-activated cell sorted sperm cells. A cDNA library was constructed by oligo dT priming using the Clontech SMART cDNA synthesis kit. The cDNAs were directionally cloned.
Source: McCormick, S., USDA/ARS
Gene Content Analysis
3,719 ESTs from this library were grouped into 1,895 UniGene entries (putative genes) [UniGene build #74, 02-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 65 | 17478 |  |
Zm.72560 | Hypothetical protein LOC100193571 (LOC100193571), mRNA. |
| 32 | 8604 |  |
Zm.14699 | Clone 1475336 unknown mRNA. |
| 28 | 7529 |  |
Zm.72713 | Clone 1479657 mRNA sequence. |
| 27 | 7260 | |
Zm.23696 | Transcribed locus. |
| 24 | 6453 | |
Zm.92368 | Clone 1216993 unknown mRNA. |
| 23 | 6184 |  |
Zm.98408 | Transcribed locus. |
| 23 | 6184 | |
Zm.26525 | Transcribed locus, weakly similar to NP_173344.2 ATP-binding region, ATPase-like domain-containing protein-related [Arabidopsis thaliana]. |
| 22 | 5916 | |
Zm.82375 | Clone 1281897 unknown mRNA. |
| 22 | 5916 | |
Zm.13940 | Clone 1705111 unknown mRNA. |
| 21 | 5647 | |
Zm.8152 | Hypothetical protein LOC100192746 (LOC100192746), mRNA. |
| 20 | 5378 | |
Zm.72628 | Clone 450965 unknown mRNA. |
| 19 | 5109 | |
Zm.1886 | Clone 210163 mRNA sequence. |
| 18 | 4840 |  |
Zm.93678 | Clone 322935 mRNA sequence. |
| 18 | 4840 | |
Zm.61926 | Transcribed locus. |
| 18 | 4840 | |
Zm.23253 | Histone H4 (H4C14), mRNA. |
| 17 | 4571 | |
Zm.92327 | Clone 345067 mRNA sequence. |
| 16 | 4302 | |
Zm.98399 | Clone 211924 mRNA sequence. |
| 16 | 4302 | |
Zm.117490 | Clone 1372150 unknown mRNA. |
| 15 | 4033 | |
Zm.80987 | Clone 240531 unknown mRNA. |
| 14 | 3764 | |
Zm.98223 | Transcribed locus, weakly similar to NP_001050780.1 Os03g0648500 [Oryza sativa (japonica cultivar-group)]. |
| 14 | 3764 | |
Zm.91576 | Transcribed locus. |
| 13 | 3496 |  |
Zm.89390 | Clone 242964 unknown mRNA. |
| 13 | 3496 | |
Zm.83587 | Clone 990656 unknown mRNA. |
| 13 | 3496 | |
Zm.82895 | Clone 1466688 unknown mRNA. |
| 13 | 3496 | |
Zm.7142 | Clone 1673628 unknown mRNA. |
| 13 | 3496 | |
Zm.4862 | Clone 1471315 mRNA sequence. |
| 13 | 3496 | |
Zm.27212 | Hypothetical protein LOC100191158 (LOC100191158), mRNA. |
| 12 | 3227 | |
Zm.93841 | Clone 228467 unknown mRNA. |
| 12 | 3227 | |
Zm.92448 | Clone 325241 mRNA sequence. |
| 12 | 3227 | |
Zm.5568 | Clone 264035 unknown mRNA. |
| 12 | 3227 | |
Zm.3981 | Clone 1690092 unknown mRNA. |
| 12 | 3227 | |
Zm.2588 | Clone 219146 unknown mRNA. |
| 12 | 3227 | |
Zm.14720 | Transcribed locus. |
| 11 | 2958 | |
Zm.94216 | Clone 208509 unknown mRNA. |
| 11 | 2958 | |
Zm.88297 | Clone 1277475 unknown mRNA. |
| 11 | 2958 | |
Zm.83692 | Clone 1583992 unknown mRNA. |
| 11 | 2958 | |
Zm.50315 | Transcribed locus. |
| 11 | 2958 | |
Zm.122712 | Hypothetical protein LOC100194198 (LOC100194198), mRNA. |
| 10 | 2689 | |
Zm.98398 | Transcribed locus, weakly similar to NP_191346.1 AHUS5 (SUMO CONJUGATION ENZYME 1); ubiquitin-protein ligase [Arabidopsis thaliana]. |
| 10 | 2689 | |
Zm.6061 | Hypothetical protein LOC100192928 (LOC100192928), mRNA. |
| 10 | 2689 | |
Zm.23357 | Transcribed locus. |
| 10 | 2689 | |
Zm.16236 | Hypothetical protein LOC100191422 (LOC100191422), mRNA. |
| 10 | 2689 | |
Zm.16065 | Clone 323328 mRNA sequence. |
| 10 | 2689 | |
Zm.14811 | Clone 282287 unknown mRNA. |
| 9 | 2420 |  |
Zm.96548 | Hypothetical protein LOC100194095 (LOC100194095), mRNA. |
| 9 | 2420 | |
Zm.93383 | Transcribed locus, moderately similar to NP_001061665.1 Os08g0374200 [Oryza sativa (japonica cultivar-group)]. |
| 9 | 2420 | |
Zm.92928 | Transcribed locus. |
| 9 | 2420 | |
Zm.81385 | Clone 356603 mRNA sequence. |
| 9 | 2420 | |
Zm.68067 | Clone 1495633 unknown mRNA. |
| 9 | 2420 | |
Zm.4017 | Clone 1468539 mRNA sequence. |
| 9 | 2420 | |
Zm.20505 | Transcribed locus, moderately similar to NP_001130134.1 hypothetical protein LOC100191228 [Zea mays]. |
| 9 | 2420 | |
Zm.122798 | Hypothetical protein LOC100193596 (LOC100193596), mRNA. |
| 9 | 2420 | |
Zm.118022 | Clone 286804 unknown mRNA. |
| 8 | 2151 | |
Zm.97552 | Clone 220102 unknown mRNA. |
| 8 | 2151 | |
Zm.94081 | Clone 321571 unknown mRNA. |
| 8 | 2151 | |
Zm.93597 | General regulatory factor1 (grf1), mRNA. |
| 8 | 2151 | |
Zm.303 | Clone 1565332 unknown mRNA. |
| 8 | 2151 | |
Zm.16484 | Clone 287893 unknown mRNA. |
| 8 | 2151 | |
Zm.14714 | Clone 1479406 mRNA sequence. |
| 8 | 2151 | |
Zm.122836 | Clone 1449648 unknown mRNA. |
| 7 | 1882 | |
Zm.94306 | Clone 425162 unknown mRNA. |
| 7 | 1882 | |
Zm.8981 | Clone 460796 mRNA sequence. |
| 7 | 1882 | |
Zm.82273 | Clone 1627027 unknown mRNA. |
| 7 | 1882 | |
Zm.80185 | Clone 705811 mRNA sequence. |
| 7 | 1882 | |
Zm.79729 | Clone 326168 unknown mRNA. |
| 7 | 1882 | |
Zm.78971 | Clone 276596 mRNA sequence. |
| 7 | 1882 | |
Zm.72659 | Clone 1280021 unknown mRNA. |
| 7 | 1882 | |
Zm.67998 | Hypothetical protein LOC100194137 (LOC100194137), mRNA. |
| 7 | 1882 | |
Zm.67339 | Clone 896746 mRNA sequence. |
| 7 | 1882 | |
Zm.356 | Clone 207854 unknown mRNA. |
| 7 | 1882 | |
Zm.26689 | Transcribed locus. |
| 7 | 1882 | |
Zm.2405 | Hypothetical protein LOC100191373 (LOC100191373), mRNA. |
| 7 | 1882 | |
Zm.1793 | Clone 1618987 mRNA sequence. |
| 7 | 1882 | |
Zm.16357 | Transcribed locus, weakly similar to NP_001050456.1 Os03g0439900 [Oryza sativa (japonica cultivar-group)]. |
| 7 | 1882 | |
Zm.14979 | Clone 297332 unknown mRNA. |
| 7 | 1882 | |
Zm.121942 | Transcribed locus. |
| 7 | 1882 | |
Zm.121794 | Clone 237372 unknown mRNA. |
| 7 | 1882 | |
Zm.118894 | Clone 502363 unknown mRNA. |
| 6 | 1613 | |
Zm.95876 | Clone 360344 mRNA sequence. |
| 6 | 1613 | |
Zm.92737 | Clone 1703035 unknown mRNA. |
| 6 | 1613 | |
Zm.84669 | Clone 208113 unknown mRNA. |
| 6 | 1613 | |
Zm.82440 | Clone 311732 unknown mRNA. |
| 6 | 1613 | |
Zm.82266 | Clone 1472828 mRNA sequence. |
| 6 | 1613 | |
Zm.70836 | Clone 1281534 unknown mRNA. |
| 6 | 1613 | |
Zm.66675 | Clone 1399641 unknown mRNA. |
| 6 | 1613 | |
Zm.5005 | Clone 1475520 mRNA sequence. |
| 6 | 1613 | |
Zm.466 | Clone 1471454 mRNA sequence. |
| 6 | 1613 | |
Zm.28530 | Clone 492463 mRNA sequence. |
| 6 | 1613 | |
Zm.28123 | Clone 236691 unknown mRNA. |
| 6 | 1613 | |
Zm.2793 | Clone 1280405 unknown mRNA. |
| 6 | 1613 | |
Zm.123244 | Clone 225624 unknown mRNA. |
| 6 | 1613 | |
Zm.118991 | Transcribed locus. |
| 6 | 1613 | |
Zm.118240 | Hypothetical protein LOC100193705 (LOC100193705), mRNA. |
| 5 | 1344 |  |
Zm.96237 | Transcribed locus. |
| 5 | 1344 | |
Zm.95925 | Clone 1453272 unknown mRNA. |
| 5 | 1344 | |
Zm.94704 | Clone 495027 unknown mRNA. |
| 5 | 1344 | |
Zm.93807 | Hypothetical protein LOC100192503 (LOC100192503), mRNA. |
| 5 | 1344 | |
Zm.93683 | Clone 277006 unknown mRNA. |
| 5 | 1344 | |
Zm.92521 | Hypothetical protein LOC100192845 (LOC100192845), mRNA. |
| 5 | 1344 | |
Zm.87312 | Clone 492601 mRNA sequence. |
Source: NCBI UniGene
EST Sequences
5,174 EST sequences from this library have been submitted to the public sequence database as of 02-Dec-2008.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- pollen
Source: NCBI Clone Registry
References
Sperm cells of Zea mays have a complex complement of mRNAs. Engel ML, et al., Plant J 34, 697-707 (2003).