I- BeLPT ANALYSIS USING LAV ID= 0271
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Treatment Grp Well Counts Residuals
FIT* CV-mad
day5 controls 1220 2391 1774 947 1453.8 34.9 -18 50 20 -43
day5 controls 1499 1568 1410 1131 1453.8 34.9 3 8 -3 -25
day5 controls 969 2265 1743 728 1453.8 34.9 -41 44 18 -69
day5 be1 1777 1890 1702 1885 1830.2 5.3 -3 3 -7 3
day5 be10 3368 7221 1473 3097 3229.7 70.8 4 80 -79 -4
day5 be100 3631 3655 2452 1634 2983.8 34.2 20 20 -20 -60
day7 controls 3616 17410 3989 3144 3018.0 84.5 18 175 28 4
day7 controls 669 1257 1497 4460 3018.0 84.5 -151 -88 -70 39
day7 controls 2897 4174 1366 1152 3018.0 84.5 -4 32 -79 -96
day7 be1 1670 2186 629 1264 1452.9 46.9 14 41 -84 -14
day7 be10 330 598 254 264 295.2 22.4 11 71 -15 -11
day7 be100 3611 4436 14452 14892 8006.8 103.7 -80 -59 59 62
PHA 102156 44221 59346 51090 55063.5 25.2 62 -22 7 -7
CONA 115673 104146 252237 159421 135796.6 36.4 -16 -27 62 16
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*FIT = Fitted Value = exp[ median(log z)] for each treatment group
Coefficient of Variation-MAD (CV-MAD) and residuals are in L% units
II- STIMULATION INDICES (SI)
Day 5 Day 7 Positve Controls
D5be1 D5be10 D5be100 D7be1 D7be10 D7be100 PHA CONA
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SI 1.26 2.22 2.05 0.48 0.10 2.65 37.88 93.41
LogSI 0.23 0.80 0.72 -0.73 -2.32 0.98 3.63 4.54
SLsi 1.00 3.48 3.13 -1.25 -3.98 1.67 15.83 19.76
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NOTE SLsi= LogSI/StERR is Standardized LogSI
Large POSITIVE value ( GT 2.5 ) Indicate POSTIVE Response
Large NEGATIVE value ( LT -2.5 ) Indicate Cell Killing
III- SUMMARY STATISTICS PHITILDE (Coefficient of Variation)*
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Overall: 0.385
Day 5 Control: 0.349 Day 5 Treated: 0.23 Day 5 Pooled: 0.319
Day 7 Control: 0.845 Day 7 Treated: 0.855 Day 7 Pooled: 0.811
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* Phitilde is median absolute deviation(MAD) estimate of the standard
deviation on log scale (corresponds to Coefficient of Variation on original scale)
Exhibit A4. LAV Report for BeLPT Assay 271