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Library:19975 (dbEST ID)
ZM_BFc
Library Description
| Organism: | Zea mays |
| Cultivar: | B73 |
| Developmental stage: | mixed |
| Tissue: | mixed |
| Vector: | pCMV.SPORT-6.1 |
| Vector type: | Plasmid |
| Cloning site 1: | EcoRI |
| Cloning site 2: | NotI |
| Lab host: | DH10B T1 phage resistant |
Maize Full length cDNA library constructed by Invitrogen from multiple tissues: 7 day seedling, 7 day seedling with 2 day salt treatment, 4 hour 45 C heat shock, 24 hour 10 C treatment, 4 hour UV-B treatment, 2 day osmotic stress, 7 day etiolated seedling and Juvenile leaf. Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; This is a Gateway compatible vector, permitting clone movement to new vector backbones for expression in diverse host cells using recombination rather than restriction enzymes. poly(A)+ mRNA was prepared by Invitrogen, and equimolar amounts of RNA from each tissue samples were mixed together for selection of mRNA with a 5' cap. After synthesis of cDNA, a normalization step was conducted against the mixture of RNA sources.
Source: Yeisoo Yu, The University of Arizona
Gene Content Analysis
108,696 ESTs from this library were grouped into 16,702 UniGene entries (putative genes) [UniGene build #74, 02-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 338 | 3110 |  |
Zm.98861 | Transcribed locus, moderately similar to NP_001062188.1 Os08g0506400 [Oryza sativa (japonica cultivar-group)]. |
| 323 | 2972 | |
Zm.95856 | Clone 304431 unknown mRNA. |
| 311 | 2861 | |
Zm.95991 | Clone 244046 mRNA sequence. |
| 289 | 2659 | |
Zm.72523 | Clone 263807 unknown mRNA. |
| 243 | 2236 |  |
Zm.84075 | Clone 360267 unknown mRNA. |
| 237 | 2180 | |
Zm.99238 | Clone 358812 unknown mRNA. |
| 204 | 1877 | |
Zm.39046 | Clone 373525 mRNA sequence. |
| 197 | 1812 | |
Zm.39708 | Transcribed locus, moderately similar to NP_001067630.1 Os11g0255300 [Oryza sativa (japonica cultivar-group)]. |
| 193 | 1776 | |
Zm.13263 | Clone 1599221 unknown mRNA. |
| 192 | 1766 | |
Zm.96704 | Clone 275171 mRNA sequence. |
| 189 | 1739 | |
Zm.76482 | Hypothetical protein LOC100191595 (LOC100191595), mRNA. |
| 136 | 1251 |  |
Zm.4914 | Clone 259593 unknown mRNA. |
| 133 | 1224 | |
Zm.67083 | Clone 1559151 mRNA sequence. |
| 123 | 1132 | |
Zm.84151 | Transcribed locus, moderately similar to NP_001061677.1 Os08g0376300 [Oryza sativa (japonica cultivar-group)]. |
| 122 | 1122 | |
Zm.93868 | Clone 1469309 mRNA sequence. |
| 122 | 1122 | |
Zm.28626 | Clone 391751 unknown mRNA. |
| 120 | 1104 | |
Zm.9197 | Clone 1000184 unknown mRNA. |
| 114 | 1049 | |
Zm.67861 | Clone 261026 unknown mRNA. |
| 113 | 1040 | |
Zm.79670 | Transcribed locus, moderately similar to NP_001065789.1 Os11g0155500 [Oryza sativa (japonica cultivar-group)]. |
| 109 | 1003 | |
Zm.94911 | Clone 1532585 mRNA sequence. |
| 109 | 1003 | |
Zm.28379 | Hypothetical protein LOC100192462 (LOC100192462), mRNA. |
| 106 | 975 | |
Zm.122129 | Clone 365873 unknown mRNA. |
| 105 | 966 | |
Zm.28264 | Clone 1533455 unknown mRNA. |
| 105 | 966 | |
Zm.14436 | Clone 219143 unknown mRNA. |
| 103 | 948 | |
Zm.94125 | Clone 336441 mRNA sequence. |
| 103 | 948 | |
Zm.2980 | Hypothetical protein LOC100193833 (LOC100193833), mRNA. |
| 102 | 938 | |
Zm.96652 | Clone 1217895 unknown mRNA. |
| 102 | 938 | |
Zm.66863 | Clone 1553967 mRNA sequence. |
| 100 | 920 | |
Zm.96834 | Clone 1062559 unknown mRNA. |
| 100 | 920 | |
Zm.94354 | Hypothetical protein LOC100191512 (LOC100191512), mRNA. |
| 100 | 920 | |
Zm.83223 | Transcribed locus, moderately similar to NP_001050281.1 Os03g0391400 [Oryza sativa (japonica cultivar-group)]. |
| 98 | 902 | |
Zm.87300 | Clone 262173 unknown mRNA. |
| 96 | 883 |  |
Zm.76433 | Hypothetical protein LOC100194154 (LOC100194154), mRNA. |
| 94 | 865 | |
Zm.83830 | Hypothetical protein LOC100191664 (LOC100191664), mRNA. |
| 93 | 856 | |
Zm.95951 | Inorganic phosphate transporter 3 (LOC732717), mRNA. |
| 93 | 856 | |
Zm.75792 | Clone 304053 mRNA sequence. |
| 92 | 846 | |
Zm.711 | Clone 233631 unknown mRNA. |
| 91 | 837 | |
Zm.96034 | Clone 324137 mRNA sequence. |
| 91 | 837 | |
Zm.36627 | Clone 402586 mRNA sequence. |
| 91 | 837 | |
Zm.17186 | Transcribed locus, moderately similar to NP_001046080.1 Os02g0178800 [Oryza sativa (japonica cultivar-group)]. |
| 90 | 828 | |
Zm.76901 | Clone 280800 unknown mRNA. |
| 90 | 828 | |
Zm.122051 | Clone 1470154 mRNA sequence. |
| 88 | 810 | |
Zm.77684 | Hypothetical protein LOC100191963 (LOC100191963), mRNA. |
| 87 | 800 | |
Zm.97214 | Clone 1544719 mRNA sequence. |
| 87 | 800 | |
Zm.66834 | Clone 262130 unknown mRNA. |
| 86 | 791 | |
Zm.9524 | Clone 365900 unknown mRNA. |
| 86 | 791 | |
Zm.93641 | Clone 1545969 unknown mRNA. |
| 86 | 791 | |
Zm.84442 | Clone 372168 mRNA sequence. |
| 86 | 791 | |
Zm.1035 | Clone 293322 mRNA sequence. |
| 85 | 782 | |
Zm.97162 | Transcription factor MYC7E. |
| 85 | 782 | |
Zm.84438 | Clone 413439 mRNA sequence. |
| 85 | 782 | |
Zm.83189 | Clone 1632418 mRNA sequence. |
| 85 | 782 | |
Zm.6914 | Clone 1560980 unknown mRNA. |
| 85 | 782 | |
Zm.1133 | Hypothetical protein LOC100191400 (LOC100191400), mRNA. |
| 84 | 773 | |
Zm.9391 | Clone 319665 mRNA sequence. |
| 84 | 773 | |
Zm.83039 | Clone 336035 unknown mRNA. |
| 83 | 764 | |
Zm.99602 | Transcribed locus, weakly similar to NP_001054807.1 Os05g0180300 [Oryza sativa (japonica cultivar-group)]. |
| 83 | 764 | |
Zm.38958 | Clone 1627572 mRNA sequence. |
| 82 | 754 | |
Zm.97495 | Transcribed locus, moderately similar to NP_001061787.1 Os08g0411300 [Oryza sativa (japonica cultivar-group)]. |
| 82 | 754 | |
Zm.93694 | Clone 1290437 unknown mRNA. |
| 82 | 754 | |
Zm.39055 | Clone 485217 unknown mRNA. |
| 81 | 745 | |
Zm.76775 | Clone 1696655 mRNA sequence. |
| 80 | 736 | |
Zm.97469 | Transcribed locus, strongly similar to NP_001061048.1 Os08g0160500 [Oryza sativa (japonica cultivar-group)]. |
| 79 | 727 | |
Zm.28638 | Transcribed locus, moderately similar to NP_001049067.1 Os03g0165300 [Oryza sativa (japonica cultivar-group)]. |
| 78 | 718 | |
Zm.95502 | Transcribed locus, moderately similar to NP_001042808.1 Os01g0297200 [Oryza sativa (japonica cultivar-group)]. |
| 78 | 718 | |
Zm.93748 | Hypothetical protein LOC100191584 (LOC100191584), mRNA. |
| 78 | 718 | |
Zm.67304 | Clone 229106 unknown mRNA. |
| 78 | 718 | |
Zm.36969 | Transcribed locus, weakly similar to NP_001065981.1 Os12g0114100 [Oryza sativa (japonica cultivar-group)]. |
| 77 | 708 | |
Zm.6278 | Clone 441778 mRNA sequence. |
| 76 | 699 | |
Zm.39727 | Full-length cDNA clone ZM_BFc0080C07. |
| 75 | 690 | |
Zm.84651 | Clone 299035 unknown mRNA. |
| 75 | 690 | |
Zm.82219 | Clone 221619 unknown mRNA. |
| 75 | 690 | |
Zm.19224 | Hypothetical protein LOC100191905 (LOC100191905), mRNA. |
| 75 | 690 | |
Zm.10036 | Clone 1560885 unknown mRNA. |
| 74 | 681 | |
Zm.96942 | Clone 374566 mRNA sequence. |
| 74 | 681 | |
Zm.81309 | Clone 272654 mRNA sequence. |
| 74 | 681 | |
Zm.28637 | Transcribed locus, moderately similar to NP_001054362.1 Os04g0691800 [Oryza sativa (japonica cultivar-group)]. |
| 73 | 672 | |
Zm.96522 | Clone 295593 unknown mRNA. |
| 73 | 672 | |
Zm.95607 | Clone 1696939 mRNA sequence. |
| 72 | 662 | |
Zm.98779 | Transcribed locus, moderately similar to NP_001051653.1 Os03g0808900 [Oryza sativa (japonica cultivar-group)]. |
| 72 | 662 | |
Zm.7 | Clone 1474197 mRNA sequence. |
| 72 | 662 | |
Zm.39723 | Clone 1559413 unknown mRNA. |
| 71 | 653 | |
Zm.93878 | Clone 240632 unknown mRNA. |
| 71 | 653 | |
Zm.76018 | Transcribed locus, weakly similar to NP_001050282.1 Os03g0391700 [Oryza sativa (japonica cultivar-group)]. |
| 71 | 653 | |
Zm.119002 | Hypothetical protein LOC100191940 (LOC100191940), mRNA. |
| 71 | 653 | |
Zm.1087 | Clone 1556801 unknown mRNA. |
| 70 | 644 | |
Zm.93926 | Clone 294726 unknown mRNA. |
| 70 | 644 | |
Zm.77025 | Hypothetical protein LOC100191539 (LOC100191539), mRNA. |
| 70 | 644 | |
Zm.32162 | Hypothetical protein LOC100194153 (LOC100194153), mRNA. |
| 70 | 644 | |
Zm.295 | Clone 278664 unknown mRNA. |
| 70 | 644 | |
Zm.122395 | Clone 220044 unknown mRNA. |
| 69 | 635 | |
Zm.94540 | Transcribed locus. |
| 69 | 635 | |
Zm.72637 | Clone 1279753 unknown mRNA. |
| 69 | 635 | |
Zm.67921 | Clone 1470257 mRNA sequence. |
| 69 | 635 | |
Zm.6459 | Clone 1705674 mRNA sequence. |
| 68 | 626 | |
Zm.93959 | Full-length cDNA clone ZM_BFb0198N01. |
| 68 | 626 | |
Zm.86924 | Clone 299836 unknown mRNA. |
| 68 | 626 | |
Zm.119548 | Clone 504579 unknown mRNA. |
| 67 | 616 | |
Zm.93693 | Clone 226119 mRNA sequence. |
| 67 | 616 | |
Zm.28732 | Clone 306228 mRNA sequence. |
Source: NCBI UniGene
EST Sequences
120,616 EST sequences from this library have been submitted to the public sequence database as of 02-Dec-2008.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- normalized
Source: NCBI Clone Registry