Srihari BJ, Jacob SM, Sundaravalli A, Lakshmi Devi B, Samuel NM; International Conference on AIDS (15th : 2004 : Bangkok, Thailand).
Int Conf AIDS. 2004 Jul 11-16; 15: abstract no. C12676.
Department of Experimental Medicine, TN Dr MGR Medical University, Guindy, Chennai, India
Background: High maternal viral load and low CD4 counts were associated with perinatal transmission. The role of virus determinats in MTCT has not been well established yet. So far there is no clear evidence for subtype specific variation in virulence or transmission; hence it is proposed to assess the role of HIV-1 subtype in MTCT in Indian setting. Methods: 62 HIV-1 seropositive pregnant women blood samples were collected in a sterile 2ml EDTA vacutainer tube from Institute of Obstetrics and Gynecology (IOG), and Raja Sir Ramasamy Mudaliar (RSRM) lying in Hospital, Chennai. These samples were transported to the Laboratory of DEM immediately after collection. HIV-1 subtyping was performed by both env and gag Heteroduplex Mobility Analysis (HMA) as per standard protocol. Total Lymphocyte counts were enumerated by Cell counter. The TLC values were correlated with CD4 with the cutoff value of 1800 cells/ul of TLC to 350 CD4 T cells/cmm. Results: The transmission rate at birth in the study group was 8.06% (5/62). The CD4 T cell count ranged between 350-933cells/cmm and 77-1205cells/cmm in transmitting and non transmitting mothers respectively. There was no significant difference in CD4 T cell counts between transmitting and non transmitting mothers (P=0.2402). Subtype C was present in 28/62 samples (23 non transmitters and 5 transmitters) initially tested. env sub-subtype C3-Indian strain (88.23%) (15/17) and gag sub-subtype C2 (DRC strain) (54.54%) (6/11) was predominant among these women. No recombinants or other than subtype C was identified in the study group. The infected infants at birth were found to have the same maternal subtype. Conclusion: In conclusion, our preliminary analysis of subtyping by HMA (Heteroduplex Mobility Analysis) of transmitting mothers and their infants had no difference in their subtypes. These samples will be further sequenced to assess the role of viral variants if any in relation to MTCT among subtype C infected women.
Publication Types:
Keywords:
- CD4 Lymphocyte Count
- Disease Transmission, Vertical
- Female
- Genes, env
- Genes, gag
- HIV-1
- Heteroduplex Analysis
- Humans
- India
- Infant
- Parturition
- Pregnancy
- Viral Load
- genetics
- transmission
Other ID:
UI: 102277646
From Meeting Abstracts