BT0407

Library Description

Organism:	Homo sapiens
Developmental stage:	Adult
Organ:	breast
Vector:	puc18
Vector type:	Plasmid
Cloning site 1:	SmaI
Cloning site 2:	SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

368 ESTs from this library were grouped into 187 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
32	86957	Hs.378103	Ribosomal protein S5.
16	43478	Hs.571186	Transcribed locus, strongly similar to NP_033121.1 ribosomal protein S5 [Mus musculus].
13	35326	Hs.269577	Protein tyrosine phosphatase, receptor type, A.
11	29891	Hs.532359	Ribosomal protein L5.
8	21739	Hs.715525	Thioredoxin interacting protein.
8	21739	Hs.657121	Spermatogenesis associated 13.
7	19022	Hs.495229	Ubiquitin related modifier 1 homolog (S. cerevisiae).
6	16304	Hs.181301	Cathepsin S.
5	13587	Hs.502770	Tumor suppressing subtransferable candidate 1.
5	13587	Hs.405987	Nucleolar complex associated 2 homolog (S. cerevisiae).
5	13587	Hs.108029	SH3 domain binding glutamic acid-rich protein like.
4	10870	Hs.707724	Transcribed locus, strongly similar to NP_000961.2 ribosomal protein L6 [Homo sapiens].
4	10870	Hs.369762	Enolase superfamily member 1.
4	10870	Hs.282975	Carboxylesterase 2 (intestine, liver).
4	10870	Hs.181368	PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae).
3	8152	Hs.593928	Farnesyl-diphosphate farnesyltransferase 1.
3	8152	Hs.591852	ADAM metallopeptidase domain 9 (meltrin gamma).
3	8152	Hs.533721	KIAA0284.
3	8152	Hs.527105	Heterogeneous nuclear ribonucleoprotein D-like.
3	8152	Hs.515003	Chromosome 19 open reading frame 6.
3	8152	Hs.513666	Chromosome 16 open reading frame 70.
3	8152	Hs.505154	Family with sequence similarity 60, member A.
3	8152	Hs.470907	Adenylate kinase 2.
3	8152	Hs.430551	IQ motif containing GTPase activating protein 1.
3	8152	Hs.180877	H3 histone, family 3B (H3.3B).
3	8152	Hs.18069	Legumain.
3	8152	Hs.159028	Butyrophilin, subfamily 2, member A1.
3	8152	Hs.119598	Ribosomal protein L3.
3	8152	Hs.11923	Yip1 domain family, member 1.
2	5435	Hs.718590	CDNA FLJ43445 fis, clone OCBBF2031366.
2	5435	Hs.718541	Heat shock protein 90kDa alpha (cytosolic), class B member 3 (pseudogene).
2	5435	Hs.709642	Neuroblastoma breakpoint family member 6-like protein.
2	5435	Hs.705889	Transcribed locus.
2	5435	Hs.656146	Hypothetical LOC441242.
2	5435	Hs.632370	Ubiquitination factor E4B (UFD2 homolog, yeast).
2	5435	Hs.613105	Transcribed locus.
2	5435	Hs.600465	Transcribed locus.
2	5435	Hs.592121	Rabaptin, RAB GTPase binding effector protein 1.
2	5435	Hs.534322	Major histocompatibility complex, class II, DR beta 1.
2	5435	Hs.530331	Pyruvate dehydrogenase (lipoamide) alpha 1.
2	5435	Hs.523739	Nuclear RNA export factor 1.
2	5435	Hs.521530	Ash2 (absent, small, or homeotic)-like (Drosophila).
2	5435	Hs.517438	Activating signal cointegrator 1 complex subunit 2.
2	5435	Hs.514870	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1.
2	5435	Hs.512461	G protein-coupled receptor 107.
2	5435	Hs.509718	Zinc finger protein 318.
2	5435	Hs.508113	Olfactomedin 4.
2	5435	Hs.503178	Spectrin, beta, non-erythrocytic 1.
2	5435	Hs.489354	Mucin 3A, cell surface associated.
2	5435	Hs.468478	Forkhead box N2.
2	5435	Hs.467807	Lysosomal protein transmembrane 4 alpha.
2	5435	Hs.459211	A kinase (PRKA) anchor protein 13.
2	5435	Hs.445890	Cornichon homolog 4 (Drosophila).
2	5435	Hs.439370	Methylthioribose-1-phosphate isomerase homolog (S. cerevisiae).
2	5435	Hs.439200	KIAA0090.
2	5435	Hs.432760	Capping protein (actin filament) muscle Z-line, beta.
2	5435	Hs.414473	Transmembrane protein 63B.
2	5435	Hs.377416	Engulfment and cell motility 3.
2	5435	Hs.323489	Pentatricopeptide repeat domain 3.
2	5435	Hs.25601	Chromodomain helicase DNA binding protein 3.
2	5435	Hs.173524	KIAA0430.
2	5435	Hs.1501	Syndecan 2.
2	5435	Hs.137556	Protocadherin 21.
2	5435	Hs.119387	Transmembrane protein 63A.
2	5435	Hs.118262	Calcium channel, voltage-dependent, L type, alpha 1C subunit.
1	2717	Hs.89643	Transketolase.
1	2717	Hs.82916	Chaperonin containing TCP1, subunit 6A (zeta 1).
1	2717	Hs.81328	Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha.
1	2717	Hs.75724	Coatomer protein complex, subunit beta 2 (beta prime).
1	2717	Hs.718404	Bladder cancer associated protein.
1	2717	Hs.716972	Transcribed locus.
1	2717	Hs.715499	Transmembrane 4 L six family member 1.
1	2717	Hs.715493	Zinc finger protein 587.
1	2717	Hs.714302	Vesicle-associated membrane protein 8 (endobrevin).
1	2717	Hs.709251	Transcribed locus, strongly similar to XP_001476854.1 PREDICTED: hypothetical protein [Mus musculus].
1	2717	Hs.700599	Wolf-Hirschhorn syndrome candidate 1-like 1.
1	2717	Hs.696153	Ring finger protein 170.
1	2717	Hs.689683	Transcribed locus, strongly similar to XP_854919.1 PREDICTED: similar to Heat shock protein HSP 90-beta (HSP 84) (Tumor specific transplantation 84 kDa antigen) (TSTA) [Canis familiaris].
1	2717	Hs.687373	Transcribed locus.
1	2717	Hs.678937	Transcribed locus.
1	2717	Hs.677658	Transcribed locus.
1	2717	Hs.675831	CDNA FLJ31233 fis, clone KIDNE2004579.
1	2717	Hs.675830	CDNA FLJ25538 fis, clone CBR09241.
1	2717	Hs.669724	Transcribed locus, strongly similar to NP_036454.1 LSM5 homolog, U6 small nuclear RNA associated isoform a [Homo sapiens].
1	2717	Hs.668919	Transcribed locus.
1	2717	Hs.667531	CDNA FLJ12415 fis, clone MAMMA1003015.
1	2717	Hs.661470	GRB2-related adaptor protein-like.
1	2717	Hs.658218	CDNA clone IMAGE:5275753.
1	2717	Hs.656849	RANBP2-like and GRIP domain containing 1.
1	2717	Hs.656630	Transcribed locus.
1	2717	Hs.655248	Lysophosphatidylcholine acyltransferase 3.
1	2717	Hs.655162	Zinc finger, CCHC domain containing 6.
1	2717	Hs.654379	CD151 molecule (Raph blood group).
1	2717	Hs.653859	Ets homologous factor.
1	2717	Hs.647041	General transcription factor II, i.
1	2717	Hs.644105	Transcribed locus.
1	2717	Hs.643683	Integral membrane protein 2B.
1	2717	Hs.634040	E74-like factor 2 (ets domain transcription factor).
1	2717	Hs.632685	TruB pseudouridine (psi) synthase homolog 2 (E. coli).
1	2717	Hs.632368	Exosome component 10.