Testing Information

Testing Status of Agents at NTP

Isoprene

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http://ntp.niehs.nih.gov/go/TS-M200028

CASRN: 78-79-5

Formula: C5 H8

Synonyms/Common Names:

  • 1,3-BUTADIENE, 2-METHYL- (9CI)

Known Uses:

MONOMER AND COMONOMER FOR ELASTOMERS, PREPARED FROM TURPENTINE, PETROLEUM PRODUCTS (MERCK 1989)

Short-Term Toxicity

  • 2-WEEK (Inhalation)  (C61825) Completed 
    • TOX-31 (NTIS No. PB95-226486)  (Toxicity Peer Review 11/17/1993)
      Toxicity Studies of Isoprene (CAS No. 78-79-5) Administered by Inhalation to F344/N Rats and B6C3F1Mice
    • Rats: F344/N; Mice: B6C3F1
  • 26-WEEK (Inhalation)  (C61825C) Report Complete 
    • TOX-31 (NTIS No. PB95-226486)  (Toxicity Peer Review 11/17/1993)
      Toxicity Studies of Isoprene (CAS No. 78-79-5) Administered by Inhalation to F344/N Rats and B6C3F1Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: MALES ONLY; R&M: 0, 70, 220, 700, 2200, 7000 PPM
  • 13-WEEK (Inhalation)  (C61825) Report Complete 
    • TOX-31 (NTIS No. PB95-226486)  (Toxicity Peer Review 11/17/1993)
      Toxicity Studies of Isoprene (CAS No. 78-79-5) Administered by Inhalation to F344/N Rats and B6C3F1Mice
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0,70,220,700,2200,7000 PPM

Long-Term Carcinogenicity

  • 2-YEAR (Inhalation)  (C61825B) Report Complete 
    • TR-486 (NTIS No. PB2000-101651)  (Peer Review 12/10/1997)
      Toxicology and Carcinogenesis Studies of Isoprene (CAS No. 78-79-5) in F344/N Rats (Inhalation Studies)
    • Rats: F344/N
    • Carcinogenesis Results
      • Male Rats Clear Evidence 
      • Female Rats Some Evidence 
    • Dose: R: 0, 220, 700, OR 7000 PPM; 50/SEX/GROUP

Special Studies

  • Absorption Disposition Metabolism Elimination (Inhalation)  (S0227) Completed 
    • Citation: DAHL ET AL. THE FATE OF ISOPRENE INHALED BY RATS: COMPARISON TO BUTADIENE. TOXICOL & APPL PHARMACOLOGY 89:237-248,1987. BOND ET AL. DISPOSITION OF INHALED ISOPRENE IN B6C3F1 MICE. TOXICOL & APPL PHARMACOLOGY 107:494-503,1991. DAHL ET AL. SPECIES DIFFERENCES IN THE METABOLISM & DISPOSITION OF INHALED 1,3-BUTADIENE & ISOPRENE. ENVIRON HLTH PERSPECECTIVES 86:65-69,1990
    • M Rats: F344/N
  • Absorption Disposition Metabolism Elimination (Intraperitoneal Injection)  (S0460) Completed 
    • Citation: BIRNBAUM, ET.AL., CHARACTERIZATION OF HEMOGLOBIN ADDUCT FORMATION IN MICE & RATS AFTER ADMINSTRATION OF [14C]BUTADIENE OR [14C]ISOPRENE, TOXICOL & APPL PHARM 100:86-95,1989.
    • M Rats: Sprague Dawley; M Mice: B6C3F1
    • Dose: 0.3, 3.0, 300, 1000 OR 3000 UMOL [14C]ISOPRENE
  • Metabolism (Intraperitoneal Injection)  (S0338) Completed 
    • M Rats: F344/N; M Mice: B6C3F1
    • Dose: PILOT 1: 33.2 MG/KG MOUSE; 66.6 MG/KG RAT; PILOT 2: 66.2 MG/KG RAT; STUDY A: 66.4 MG/KG RAT; STUDY CM: 68.4 MG/KG MOUSE.
  • Modeling  (S0648) Completed 

    Genetic Toxicology

    • Chromosome Aberrations  (A63977) Completed 
      • Mice
      • Chromosome Aberation  Negative (Nonstandard Protocol) 
    • In Vitro Cytogenetics (CA/SCE)  (032841) Completed 
      • Chromosome Aberation Negative 
      • Sister Chromatid Exchange Negative 
    • Micronucleus  (A28566) Completed 
      • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
      • Mice: B6C3F1
      • Male Positive 
      • Female Positive 
    • Micronucleus  (A60010) Completed 
      • Male Positive 
      • Female Positive 
    • Micronucleus  (A63977) Completed 
      • Mice: B6C3F1
      • Male Positive 
    • Salmonella  (453451) Completed 
      • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
      •  Negative 
    • Sister Chromatid Exchanges  (A63977) Completed 
      • Mice
      • Sister Chromatid  Positive (Nonstandard Protocol) 
    • Sister Chromatid Exchanges  (C78795) Completed 
      • Citation: Tice, R.R., Boucher, R., Luke, C.A., Paquette, D.F., Melnick, R.L., and Shelby, M.D. Chloroprene and isoprene: Cytogenetic studies in mice. Mutagenesis. Vol. 3 (1988) 141-146
      • Mice
      • Sister Chromatid  Positive 

    Organ Systems Toxicity

    • GD 6 - 17 FOR MICE Conventional Teratology (Inhalation)  (TER88045) Completed 
      • F Mice: Swiss
      • Mice Maternal Positive 
      • Mice Fetal Positive 
      • Dose: 0, 280, 1400, 7000 PPM
    • GD 6-19 Conventional Teratology (Inhalation)  (TER88142) Completed 
      • Rats: Sprague Dawley
      • Rats Maternal Negative 
      • Rats Fetal Negative 
      • Dose: 0, 280, 1400, 7000 PPM

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    Web page last updated on January 22, 2009