Assembly of the Mitochondrial Membrane System. Purification of a Mitochondrial Product of the ATPase

Proc Natl Acad Sci U S A. 1973 November; 70(11): 3155–3159.

PMCID: PMC427191

Assembly of the Mitochondrial Membrane System. Purification of a Mitochondrial Product of the ATPase^*

Marcelino F. Sierra and Alexander Tzagoloff

St. John's University, New York, N.Y. 11439

The Public Health Research Institute of the City of New York, Inc., New York, N.Y. 10016

^* This paper is no. XI of a series. The preceding paper in this series is ref. 16.

This article has been cited by other articles in PMC.

Abstract

A low-molecular-weight protein synthesized in yeast mitochondria was purified. The protein was identified as a component of the rutamycin-sensitive ATPase. The amino-acid composition of the purified protein shows an extremely large preponderance of nonpolar residues, which may account for its solubility in chloroform-methanol. Indirect evidence suggests that this component is involved in the conferral of rutamycin sensitivity and may also have a function in the assembly of the ATPase complex.

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Selected References

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