Jones D, Taborda MA, Taborda M, Lupo SH, Fay OH; International Conference on AIDS.

Int Conf AIDS. 1998; 12: 847 (abstract no. 42368).

Center of Technology in Public Health, Rosario, Argentina.

OBJECTIVE: To study to response to oxidative stress testing the activities of superoxide dismutase (SOD) and Glutatione peroxidase (Gpx) in HIV patients related to CD4/CD8 rate. MATERIALS AND METHODS: 21 patients HIV(+) were included. Mononuclear cells isolated by Fycoll-Hypaque gradient separation; lysed by sonication; and activity of Cu-Zn SOD (by kinetic method Ransod), Gpx (by kinetic methods Ransel) and total proteins (TP) (Pirogallol red Randox) were tested. The CD4 and CD8 counts were performed by flow citometry (ORTHO-D). Patients were classified in Group 1 CD4/CD8 < or = 0.1 (n = 6) Group 20.1 < CD4/CD8 < or = 0.4 (n = 7) and Group 3 CD4/CD8 > 0.4 (n = 8) RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: 1) SOD/TP and GPx/TP showed significant difference among groups (p < 0.05). 2) The increment of SOD in the group 1 could be explained by an adaptive response induce by a higher exposure to Reactive oxygen species. 3) The low level of GPx in the same group, could be the result of an oxidative equilibrium quantitatively different between activation and inactivation of the SOD. More extensive study should be done in order to confirm our results.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Animals
• Antigens, CD4
• Antigens, CD8
• Antioxidants
• Argentina
• HIV Infections
• HIV Seropositivity
• Humans
• Oxidants
• Oxidation-Reduction
• Oxidative Stress
• Reactive Oxygen Species
• Superoxide Dismutase
• blood
• immunology

Other ID:

• 98404352

UI: 102231166

From Meeting Abstracts