ABSTRACTS
OF ORAL PRESENTATIONS
DEVELOPMENT AND USE OF DOUBLED
HAPLOIDS IN OATS
Taing Aung
Cereal Research Center, Agriculture
and Agri-Food Canada, 195 Dafoe Road, Winnipeg, MB, Canada, R3T 2M9
Various doubled haploid production procedures
such as several wide crossing methods and anther culture techniques have
been developed and these methods have provided opportunities in establishing
doubled haploid lines and highly homozygous breeding populations for rice,
barley, and wheat. Hitherto, there has been no report on the development
of an efficient procedure to generate a large population of doubled haploids
in oat (Avena sativa L) . Since the development of the method based
on the utilization of maize pollen to generate haploid and doubled haploid
plants in wheat, the procedures have been applied by many wheat geneticists
and breeders in producing doubled haploid lines. Successful production
of haploid (H) and doubled haploid (DH) oat through the use of maize pollen
has been reported by Rines and Dahleen in 1990. An oat doubled haploid
program was initiated at Cereal Research Center (CRC) in the fall of 1996
following the successful completion of the establishment of the wheat doubled
haploid production system. Application of maize pollen to fertilize oat
female gametophytes and subsequent treatment with 2,4-D, a method very
similar to the one we used in our wheat doubled haploid program, has resulted
in the production of 213 haploid plants from nine oat cultivars.
Nine genotypes of cultivated oat ( AC
Marie, AC Preakness, Calibre, Dal, Derby, Dumont, Riel, Robert and SunII
) were used in our project. A dwarf genotype of maize (1-1.2 m. tall) that
can be grown under normal growth cabinet conditions was developed at from
the F3 generation of a triple cross involving Seneca 60, Manitoba-Dwarf/Sweet
and Indian Flint corn and the synthetic maize genotype so produced was
designated as SDF 1. The oat genotypes were hand-emasculated and were pollinated
with freshly shed pollen of the synthetic maize genotype SDF 1. The pollinated
florets were sprayed with 2,4-D (1mg/L) solution, embryos were dissected
from 15-day old caryopses and the embryo rescue producers were performed
in Gamborg5 media. Haploid plantlets developed from the embryos were transferred
to12.7cm pots and the plants at three-tiller stage were treated with colchicine.
Approximately 30 % of the plants were treated with colchicine to induce
chromosome doubling. Mitotic chromosome counts and chromosome pairing analysis
at meiosis were carried out. The haploid plants of AC Preakness, Dumont
and SunII were used as the pistillate parent and hybridized to their respective
hexaploid cultivars as pollen parents with the aim to generate aneuploid
plants.
A total of 319 haploid seedlings with
fully developed leaves and that did not express abnormal morphological
features were generated and transferred to soil in 12.7cm pots, out of
which 213 (67 %) developed into normal plants. Out of 213 normal haploid
plants 63 plants were treated with colchicine at three-tiller-stage and
54 mixoploid {a plant with a mixture of chromosome doubled tillers and
non-doubled tillers} plants were generated (86% of the plants survived
the colchicine treatment). Of the remaining non-treated 150 haploid plants,
144 (96 %) reached full maturity with normal morphological features. All144
haploid plants were small, about 50% the size of their respective hexaploid
parents but the haploid plants tillered profusely (over 50 tillers were
recorded at the time of harvest in about 40% of the plants). The mixoploid
plants (54 plants) produced less tillers than the haploids, but several
colchicine-doubled tillers produced large fertile florets.
Record on fertility of 139 mixoploid
and haploid plants of four cultivars (68 plants of AC Preakness, 37 plants
of Dumont, 22 plants of Derby and 12 plants of Dal ) is presented in this
paper. Out of the 35 colchicine treated haploid plants 32 plants (91%)
produced 3-13 fertile tillers and produced more than 30 seeds per plant,
two plants (6%) produced less than 30 seeds, and one plant (3%) had no
seed. Among the non-treated 104 haploid plants, only 14 (13%) produced
more than 30 seeds, 34 (33%) produced less than 30 seeds and 56 (54%) had
no seed. Seeds harvested from the fully fertile tillers of the mixoploid
plants were as large as those produced on normal hexaploid plants, and
the plants generated from these seeds were normal, vigorous and all the
tillers were fully fertile with 42 chromosomes, indicating that doubled
haploid (DH) plants were generated. A total of 10 haploid plants and 11
DH plants were involved in mitotic and meiotic analyses. Mitotic
chromosome counts revealed that two maize chromosomes were detected in
the regular haploid chromosome complements (2n=21+ 2 maize) in two of the
ten haploid plants, the remaining eight were haploids with regular 21 chromosomes
with no indication of the presence of maize chromosome. Somatic chromosome
number of all the 11 DH plants was 42, with no discernable irregular chromosomes
and no maize chromosomes were detected. At the metaphase stage of meiosis
in eight haploid plants 21 univalents were formed and no chiasmate association
was observed.. Chromosome pairing at meiosis in all the DH plants was normal
with 21 bivalents. A total of 13 hybrid seeds were generated from crosses
involving haploid plants (2n=21) and euploid plants (2n=42) plants of AC
Preakness, Dumont and SunII. Two plants of SunII were hypoaneuploids with
41 chromosomes and one plant was a euploid (2n=42). One hypoaneuploid was
isolated from five plants developed from seeds harvested from SunII haploid
plants.
Fritz Matzk in1996 also had reported the
production of oat doubled haploid plants through the application of maize,
pearl millet and eastern gamagrass pollen, and 4 haploid plants were generated
from 21823 florets of oat pollinated (0.018% success). Matzk concluded
from his results along with those reported by Rines and Dahleen in 1990,
that the oat x maize system or other crosses cannot be used effectively
for haploid or doubled haploid production in oat. The efficiency of our
method in oat doubled haploid production (fully developed haploid plants
per 100 pollinated florets) for nine oat cultivars was on average 1.2%.
AC Preakness appears to be the best genotype for producing oat doubled
haploids ( 2.6 %). Riel performed poorly with less than 0.2%, showing that
different oat genotypes exhibit different response to the maize pollen-mediated
double haploid production procedure. Spontaneous chromosome doubling in
oat haploid plants was reported by Rines et al. in 1996, and seeds were
harvested from these plants. Our data indicated that most oat haploid plants
are basically sterile and when seeds were produced, the number of seeds
produced was very low in most plants (33% produced <30 seeds/plant and
54% did not produce any seed). Treating the haploid plants with colchicine
to induce chromosome doubling is an essential step in oat double haploid
program since 86 % of the haploid plants survived the treatment and 91
% of the plants that survived produced sufficient amount of seeds (>30
seeds/plant). In comparison, only 13 % of the non-treated haploid plants
produced sufficient amount of seeds (>30 seeds/plant) which was probably
due to spontaneous chromosome doubling in some tillers.
Conclusion
1. In certain oat genotypes an appreciable
number of doubled haploid (300+ ) can be produced in 12 months time by
one full time technician using two growth cabinets.
2. There is still room for improvement
in our oat doubled haploid production system.
3. For some oat genotypes maize pollen
mediated double haploid production system can be used in breeding programs.
Acknowledgements
The author thanks Drs. Scott Duguid
and Douglas Brown for their valuable advice and also for sharing their
resources. Funding support for this project from all the members of The
Oat Consortium, Canada, is gratefully acknowledged.
OAT TYPESs - WHERETO?
P. D. Brown and S.D. Duguid
Cereal Research Centre, Agriculture
& Agri-Food Canada, 195 Dafoe Road
Winnipeg, MB, Canada R3T 2M9
Oat has been a valued crop for hundreds
of years. Initially, the seed, eaten as a gruel, was a major component
of the human diet. It evolved to an animal feed and was particularly important
during the era when horse power was used for transportation and industry.
Oat straw, oat forage and the fact that oat fits into the crop rotation
has also given value to the oat crop. In recent years, however, the popularity
of oat as a component in human diets has increased. It is likely that within
the next 10-25 years oat will continue to have increased value in the human
diet.
To continue to be grown, oat must be economically
competitive relative to other crops. The question that must be asked is
"what should we do to keep oat competitive?" In most cases, the foundation
of a good oat already exists - all that remains is to make small but important
improvements in the plant type, kernel type or chemical composition of
the seed.
Several agronomic improvements would increase
the value of oat. Increases in yield potential should continue. In areas
where the growing season is short, the numbers of days from planting to
maturity should be decreased, ideally with no sacrifice in yield. Lodging
resistance must be improved; it is likely that in areas where the oat straw
is of limited value, semidwarf oat will be released and grown. Ongoing
improvements to the disease resistance of the oat plant will also maintain
its competitiveness.
Several changes to the oat kernel morphology
would also be desirable. A short plump kernel would improve seed density,
test weight, and increase transportation efficiencies. For industrial processing,
a uniform kernel shape of all primary and secondary kernels would improve
efficiency and, hence, value. Unless a valuable use for the hulls could
be found, it would be desirable to reduce the hull content. Because value
is a perception, a bright white coloured hull would be desirable. In some
markets, a naked oat, especially a hairless naked oat, will be in demand.
The chemical composition of the oat kernel,
including factors such as the amount and quality of the oil, protein, starch
and beta glucan will also be altered in different breeding programs. These
components will influence the manufacturing characteristics of the oat
kernel to add value to the oat crop.
To speculate about future oat types without
having wild dreams would be an injustice. It would be good to develop oat
varieties with increased drought tolerance or increased moisture tolerance
or reduced fertilizer dependency. Perhaps a perennial oat could be developed.
Discovering new end uses for the oat plant or oat seed would increase its
demand and value. An exciting new food could markedly increase demand.
Could oat be used as a crude oil substitute, or could it be used in the
housing industry? Oat is already being used in the fashion/cosmetic industry
but with some collaborative research, additional novel uses in this area
might be found.
To develop these new oat types 10 years
from now requires that the crosses be made immediately. To develop new
types in 25 years requires that the germplasm for these changes now be
identified for incorporation into breeding programs.
PHYSICAL DETERMINATION OF OAT QUALITY
Douglas C. Doehlert
USDA-ARS Wheat Quality Laboratory,
Harris Hall, North Dakota State University
Fargo, ND 58105 USA
Oat quality is defined by its end use.
Different end uses dictate different quality specifications. Overall, oat
quality is measured from the test weight, groat percentage, whole kernel
characteristics (size, color, uniformity), and groat characteristics (size,
hardness, uniformity, composition). Test weight is by far the most commonly
used oat quality parameter. The ease and simplicity of the test weight
measurement, along with its correlation with groat percentage, make it
an important test for oat quality evaluation. Although it is designed to
test the density of an oat kernel, the shape of the kernel also affects
test weight in a way not related to quality parameters. Image analysis
is developing as an alternative to test weight, where digitized images
of oat kernels, along with their mass can provide kernel density measurements
highly correlated with groat percentage and test weight. Although many
other types of information can be derived from image analysis, it requires
more capital equipment and time to process and is unlikely to replace test
weight in the near future. Groat percentage represents the economic yield
from a given sample of oat, but its value is strongly affected by the method
used to measure it. Errors in groat percentage can occur from hulls remaining
after dehulling, over aspiration to remove hulls, or from excessive breakage
of groats during dehulling. Oat whole kernel size can be evaluated from
thousand kernel weights, sieving on slotted sieves, or by image analysis.
An advantage of image analysis is that uniformity of kernel size is also
evaluated. Hull color is of relatively little importance, except in some
specialty markets, and is evaluated visually. Groat size can be measured
by thousand kernel weight, image analysis, or by an automated kernel analyzer.
Both the automatic kernel analyzer and image analysis provide uniformity
information, although in different units. Resistance to groat breakage
during dehulling is defined as groat hardness and is a relatively new quality
factor. Groat hardness can be measured directly from breakage after dehulling,
by an automated kernel analyzer, or by bran yield. Because kernel hardness
controls particle size in flour after grinding, higher bran yields indicate
harder groats. Economically important components of groat composition include
starch, protein, oil, and beta-glucans. Oat protein is nutritionally complete,
and higher concentrations of protein are frequently considered desirable.
Lower oil concentrations are usually desired for milling because of the
perception that this may provide better shelf life for products. Higher
oil oat products may have improved flavor and have fatty acid profiles
more desirable for stability. Beta-glucan is responsible for the cholesterol
lowering effect in oat and higher concentrations are frequently desired
for food applications. Each chemical component can be determined by a variety
of specific chemical means. Near Infrared Reflectance (NIR) is frequently
used as a fast means to estimate many chemical components simultaneously.
Care needs to be taken with NIR analyses to check some results by independent
means to assure reliability of calibrations. Any quality measurement will
vary from laboratory to laboratory. Measurements on new material should
be compared relative to cultivars of known character for improved reliability.
OAT PRODUCTION IN ZERO-TILLAGE
SYSTEMS
Martin H. Entz
Associate Professor, Department of
Plant Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
m_entz@umanitoba.ca
Renewed interest in oat production in the eastern Canadian prairies
has increased demand for technical information. One question regards the
suitability of oats to zero-tillage production systems. This paper reviews
oat productivity under zero-tillage and explores how the zero-tillage soil
environment affects growth and development of oat plants. Oat grain yields
under zero-tillage are comparable with those in tilled systems. In one
Manitoba study, oat yields averaged 4334 kg/ha under zero-tillage vs. 4288
kg/ha in a tilled system. The yield advantage of zero-tillage increases
as water becomes more limiting. The success of zero-tillage oat production
appears to be limited more by indirect factors such as weeds (especially
wild oats) and high levels of oat crop residue, rather than by physiological
responses of oats to zero-tillage per se. Studies in western Canada
and elsewhere have shown that weeds are strongly influenced by crop rotation;
crop rotation is often more important than tillage system in determining
wild oat infestations. The influence of various annual crop rotations on
wild oat and other weeds will be discussed. Forage leys provide excellent
wild oat control, especially when used in conjunction with zero-tillage.
The role of perennial and annual forages in oat rotations will be reviewed.
Oats produce more non-grain residue than most other small grain cereal
crops. In one Manitoba study, straw residue (not including chaff) averaged
5750 kg/ha for oats, 4540 kg/ha for wheat and 3900 kg/ha for barley. Under
dry conditions, this residue helps conserve soil moisture and thereby increases
productivity of the system. However under wetter conditions, this residue
can cause problems for crop seeding the following year, especially under
zero-tillage. Semidwarf lines included in the study produced straw residue
levels similar to the tall genotypes, and therefore did not provide a solution
to the residue problem. Management approaches for dealing with oat residues
in zero-tillage systems include straw baling, straw burning, or seeding
a residue "tolerant" crop such as field pea or fababean after oats.
COOPERATIVE APPROACHES TO RESEARCH, PRODUCTION, PROCESSING AND
MARKETING - THE PROCESSOR'S PERSPECTIVE
D.G. Goslin
The Quaker Oats Company of Canada Limited
All of us are in sales whether we sell goods such as cars, oatmeal,
seed or services such as scientific research, computer services or access
to government services. If what we sell meets the needs of our customer/consumer
we will be successful, and will be adequately compensated for the goods
or services we provide.
The oat processor has many customers including
the consumer, the retailer and also the shareholders of the Company. Each
of these client groups has a specific set of needs which must be met in
order that your business may grow and prosper. The consumer wants
a product that is competitively priced, has an appealing flavour/texture,
is safe to consume, and meets their nutrition and health needs. The retailer
wants a product that is competitively priced, meets their customer's needs
and therefore generates volume through their stores, and one that is supported
with effective marketing programs. The shareholder must achieve
an adequate return on their investment, and in recent years they are becoming
much more interested in corporate philosophy and a company's record on
social and environmental issues.
As the major input for all oat processors, the oat has a major impact
on how successful we are in meeting the needs of our customers. The processor
can control some of the factors which impact on customer satisfaction,
but the balance required to meet the needs of all our customers and be
successful requires that all of the factors be under control. Many of these
factors are outside the direct control of the processor, and that is where
the "cooperative approaches to -----" will allow us all to be successful
in meeting the needs of our customers.
TRENDS IN OAT PATHOLOGY
D. E. Harder
Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe
Road, Winnipeg, MB, R3T 2M9
One could list about 75 specific maladies affecting oat, ranging from
symptoms caused by infectious diseases to environmental/nutritional or
physiological factors. Any of these, although they may be rare, could seriously
affect oat production given the appropriate circumstances. This paper will
highlight some of the more common problems that may occur in North America,
the current status of some of the more important diseases where there is
on-going research, and efforts to provide solutions.
While some projects in oat pathology research are still carried out
at various institutions in North America, research involving oat diseases
generally has declined along with the decrease in oat production. Most
research currently involves crown rust, stem rust and barley yellow dwarf,
with some presence retained in the smuts and powdery mildew. Almost all
of this effort involves disease resistance - searches for new and more
effective or more durable sources of resistance, molecular markers, and/or
evaluation of resistance in breeding programs.
One of the more pressing problems at present is crown rust. The causal
fungus has shown enormous variability in virulence, and this variability
appears to be increasing. In some years recently a different pathotype
has been identified for each of about two field collections (125 pathotypes
from 260 collections in 1995). A large number of resistance genes have
been isolated from wild Avena sp. since the mid 1960s, but virulence
to all of these genes has been detected. In many cases virulence was detected
before the genes were knowingly deployed. This raises some questions as
to the origin of virulence in this and probably other rusts. The alternate
sexual host for crown rust, Rhamnus cathartica, is widespread in
the Great Plains region, and most likely contributes to "stirring the pot"
of virulence in this fungus. The result has been a rapid loss of resistance,
and when combined with high disease levels in some years recently, there
have been serious concerns about potential losses in yield and quality.
Previous optimism that a large pool of resistance genes was available into
the future has been short lived. Numerous Iberian wild oat accessions have
shown resistance in greenhouse tests, and there may be useful untapped
resistance available in this collection. Much of this resistance, however,
occurs in the lower ploidy species, thus it is more difficult to access.
The other approach which is attracting attention is to look for more durable
resistance, such as adult plant resistance or the "slow rusting" phenomenon.
Neither of these approaches has yet had a practical benefit in oat breeding,
but efforts are being made to better characterize these resistances. Molecular
techniques involving tracking genes in complex crosses, quantitative trait
analysis, and doubled haploids may assist in ultimately improving the durability
of crown rust resistance.
The stem rust situation is in sharp contrast to that of crown rust.
The Great Plains stem rust population has been very stable for over thirty
years, with only a few pathotypes predominating. Currently used resistance
sources, primarily gene Pg13, have remained effective in the prairie
region. This gene, however, is not useful in eastern regions. The effectiveness
of Pg13 may be protected by continued use of gene Pg2. The
gene Pga complex occurs quite extensively in Mexican germplasm,
and virulence to this gene has been detected in collections from the prairie
region in each of the past four years. This could cause a problem if gene
Pga should become more widely deployed. Should Pg13 fail,
there is excellent resistance available in Pg16 and Pg10.
Gene Pg16, however, with its apparent adherent A. barbata
chromatin, is associated with some yield problems Gene Pg10 has
shown moderate resistance in seedling tests, and has shown adequate resistance
in field tests. There is no known virulence to this gene in North America.
This gene also appears to enhance the effectiveness of other Pg
genes, and should be a useful addition to the resistance pool.
Barley yellow dwarf (BYD) may be more serious than is often recognized.
This disease is widespread virtually everywhere that oat is grown. Although
moderately to severely infected plants are obvious to the informed observer,
infections are often missed, or may be attributed to other causes under
field conditions. Although foliar symptoms may not always be clearly apparent,
infection by the causal virus may have profound effects on root mass. This
exacerbates the effects of the disease, particularly under stressed conditions,
such as drought. The only practical means to reduce the effects of BYD
virus infection is to provide cultivars with increased tolerance. True
resistance appears to play only a minor role. Tolerance, where plants are
able to function better under disease conditions, varies considerably among
various oat cultivars or germplasm lines. Improved performance through
increased tolerance under BYD stress has been the result of an incremental
process, involving several interacting genetic factors. Further incremental
improvements continue to be made. One of the constraints to breeding for
improved performance has been the availability of reliable small scale
indoor tests for use on earlier generation material. By equalizing and
optimizing the physical environment and using high disease pressure, it
has been shown that plant height and panicle mass are reliable predictors
of field performance. By applying these techniques at the F2
stage, plants that performed better than either of the selected tolerant
parents have been identified, indicating transgressive segregation. A further
benefit is that the improved performance in plants selected by this test
is not BYD specific, but is a more generalized phenomenon, thus these selections
should show improved yields in response to other disease or environmental
stresses.
NUTRITION FOR HUMANS AND ANIMALS
David M. Peterson
Cereal Crops Research Unit, Agricultural Research Service, United
States Department of Agriculture, Madison, Wisconsin USA, and Department
of Agronomy, University of Wisconsin-Madison
Oat is recognized as a nutritious cereal for humans and livestock.
Oat has a high percentage of relatively well balanced protein and is high
in oil and dietary fiber. It is a good source of certain vitamins and minerals,
although for prepared breakfast cereals, fortification is usually practiced.
The soluble fiber (b-glucan) of oat is known
to reduce cholesterol in humans, but also can cause some problems as a
livestock feed, especially for young poultry. Plant breeders are working
to manipulate the b-glucan levels of oat to
create special purpose cultivars.
The high oil concentration in oat, averaging about 6%, is a source
of high energy but can lead to flavor problems in processed oat. Breeders
have succeeded in almost tripling oil levels, and these high-oil genotypes
could be developed into adapted cultivars if a demand for their use develops.
Entries in the National Small Grains Collection, maintained at Aberdeen,
Idaho, are being evaluated for their concentrations of protein, oil, and
b-glucan. The data are entered into the GRIN database.
The current interest in dietary antioxidants has led to research characterizing
the antioxidant activity and the spectrum of antioxidant compounds in oat,
including phenolics and tocols. The potential for increasing antioxidant
activity is yet to be explored.
The development of new, higher yielding hulless cultivars may lead
to new uses for oat as food and feed. Hulless oat has higher energy for
livestock feed. The potential of a high-oil, hulless oat for aquiculture
should be investigated. Exploratory work has addressed the feasibility
of malting hulless oat for a food malt product.
Possible new developments that might add nutritional value to oat are
the introduction of genes for low phytic acid or waxy starch. This will
be more difficult to accomplish in hexaploid oat than in diploid cereals.
THE CHANGING CANADIAN SCENE: MARKET FACTORS BEHIND THE SHIFT
IN OAT PROCESSING TO WESTERN CANADA
Trevor Pizzey
Can-Oat Milling, Portage-La-Prairie, Manitoba
Human consumption and oat processing has seen rapid growth in Western
Canada since 1980. A number of market factors have contributed to this
concentration of value-added processing on the Prairies:
* a shifting raw material production region
* declining processing costs relative to traditional processing areas
* an evolving processed goods market
* increasing regional byproduct value
* changes to North American transportation fundamentals
Although economic factors will continue to change, the current global
trade environment is such that the concentration of human consumption oat
processing on the Prairies can be expected to continue.
FRACTIONATION AND END USES
Mark J. Redmond
Ceapro Inc., Edmonton, Alberta, Canada
After centuries of oat milling, the basic concept of dry milling is
being challenged by new and innovative separation technologies. New technologies
have introduced new products and oat extracts have found applications in
new and non-traditional markets. These markets include functional foods,
nutraceuticals, cosmetics, and consumer health products.
For the last twenty years, developments have been led by three teams
based in the United States, Finland, and Canada. Team members in each case
represented members of federally funded institutions, academia, and industry.
Process patents have been issued protecting the core of each activity and
will be presented in overview and compared (1,2,3).
The new fractionation processes have been employed with various degrees
of success and oat extracts have entered the marketplace. As with all new
products, the adoption of the oat extracts is following the typical product
life cycle, and early stage sales have been slow. However, the potential
for strong growth is now apparent, and the need for further advanced processing
facilities is under review in Europe. The presentation will highlight the
technological and business advances in oat processing.
References:
1. Bell et al (1976) U.S. Patent 4,089,848
2. Collins and Paton (1992) U.S. Patent 5,169,660
3. Lehtomäki (1992) 5,106,640
TECHNICAL WAYS TO NEW AND BETTER VARIETIES--THE BREEDERS' VIEW
Deon Stuthman
Dept. of Agronomy and Plant Genetics, University of Minnesota,
St. Paul, MN
As we approach entering the next century, it is appropriate and indeed
prudent to reflect upon what we have observed and learned from almost a
century of oat production, and oat improvement research. Since shortly
after the middle of this century, we have observed a decline in area in
the US devoted to the production of oat grain. Some people attribute much
of that decline to the elimination of literal horse power by the tractor.
However, I believe we have more horses in the US today than ever before.
Another major change in oat production is that 50 years ago, most oats
were grown with a minimum of preplant tillage. Today most are planted with
a drill into a nearly ideal seed bed. A half century ago the oat crop did
not have the assistance of broad-leaf herbicides to battle those weeds.
What have we learned about oat variety improvement since it was begun
in earnest some 80 years ago? Why do we still have rust resistant genotypes
succumb to new virulences of the pathogen, sometimes very soon after the
new variety is released? Yes, we have made some major gains in grain quality
and lodging resistance (if the soil fertility is modest), but we have also
done a lot of "reinventing the wheel", especially for crown rust resistance.
We have increased grain yield dramatically when measured in experimental
plots on Experiment Stations, but what about on many farmer fields? Has
the "state/province average oat grain yield" kept pace with the yield increases
measured on Experiment Stations? If not, why not? Have we really asked
the proper research questions to elicit answers useful to commercial oat
producers?
Do any of these observations have anything to do with my being frequently
asked, "Why don't oats of today yield like they used to"? Have we in the
process of conducting breeding yield trials in a garden with many of the
weeds eliminated, lost some useful seedling/vegetative vigor? Have we sacrificed
some ecological fitness along the way?
I have been asked to give a breeder's view on ways to new and better
varieties. Because the two speakers following me will address contemporary
technologies, most of my comments will be directed to different varieties
which are better suited to current and anticipated situations and how we
might develop them. I would also preface my remarks by a comment made in
this very city little more than one year ago at the AOA annual meeting.
A major Canadian oat producer who some of you know quite well said, "Oats
is a poor man's crop". The validity of that statement is illustrated with
a quote for an early spring crop report from one of our county educators
in Northwest Minnesota (Pennington County).
"We will be backing away from wheat and barley this year on a fairly
large scale. Those folks who have operating money are switching to soybeans
and canola. Those folks who don't, are planting oats and flax. They won't
use fertilizer and very little chemical and that will keep the cost to
a minimum."
Now that I have your attention, but perhaps not your agreement, I am
going to ask you to join me in some futuristic plant breeding thinking.
Martin Apple, Executive Director of the Council of Scientific Society Presidents,
compared what I am asking you to do to watching Channel 3 on his TV setup.
Channel 1 on his TV deals with the next year, channel 2 looks ahead 5 years
and channel 3 addresses 30-50 years from now. Given the time it takes after
a cross is made to produce a new variety when all goes well, some channel
3 thinking about new kinds of oat varieties and about new methodologies
to develop them is certainly in order.
As I was revising my five year research plan two years ago, I adopted
several quite novel breeding objectives. First the variety development
component of my project is now focused on oat varieties that will fit into
a more ecologically sound cropping system. That means among other things
that my selections should perform better in the rotations used by the growers
I am serving and with less purchased inputs thereby reducing cost of production,
but without lowering production.
Specifically, I am looking for more durable disease (especially crown
rust) resistance, for genotypes which are more competitive with weeds (especially
grassy weeds), and for genotypes which will establish adequate stands when
planted with no pre-plant tillage.
We are well on our way toward durable crown rust resistance--you will
see some evidence later this week. Although few if any North American oat
growers actually expend resources to protect their oat crop from rust,
they experience indirect increased costs because of rust in terms of reduced
gains in grain yield because of the continued efforts required for traditional
types of rust resistance.
We have made a few crosses between putative competitive and non-competitive
plants, enough to know that achieving quicker and more dense canopy cover
through selection is feasible. We have also seeded one of our on-farm yield
trials with a no-till drill. So far, some years no-till planting works
better than others, but several Brazilian oat breeding programs seed all
of their nurseries no-till because that is how Brazilian farmers grow oats,
quite successfully I might add.
Regarding crop rotations, the Brazilian situation offers us another
lesson about oat production possibilities. Much of the oats grown for grain
there is part of a double cropping system. Oats have the dual advantage
relative to wheat of being resistant to both aluminum toxicity and take-all
disease and being earlier. Thus, now that there are oat varieties available
which are earlier and also tolerate well no-till planting (which also saves
time between crops), oats are used in front of soybeans instead of wheat.
Anecdotally, oats (compared to wheat) also reduce the herbicide requirements
for the soybean crop when the soybeans are planted no-till, yet another
illustration of the usefulness of a holistic analysis of how to improve
the oat crop instead of looking at it as an isolated enterprise.
Now I want to shift to increasing the value of the oat products that
we do produce. Perhaps the greatest value of oats in the next century will
continue to be as a food ingredient. For that end use, the value of the
grain can be increased in one of at least two ways. First, the oat grain
will increase in value when it is more efficiently milled or processed.
For oats, dehulling efficiency is principally a physical consideration
and of particular importance is uniformity of grain size and perhaps shape.
All else being equal, grain of uniform size will dehull more efficiently
than grain of varying size and thus have higher milling yield. One big
contributor to uneven size is tertiary kernels.
The second way to increase the value of oat as a human food ingredient
is to increase the desired components (e.g. soluble fiber) and decrease
the "junk", i.e., fat. Both of these considerations should be obvious.
In addition, lowering the fat content also has the possible advantage of
extending product shelf-life. Much has been made about the benefits of
soluble fiber in oat relative to heart health; however, there are likely
other more subtle but equally valuable constituents which at the moment
are not well defined. Therefore, given the health consciousness of consumers,
we probably should be joining forces with the medical research community
to identify and then increase such constituents. There may also be yet
undefined, but highly valuable industrial uses for oat byproducts.
In summary, regarding breeding objectives, my suggestions are two-fold:
first, reduce the cost of production by lowering the need for purchased
inputs while holding productivity constant, and second, increase the value
of what is produced by making the grain a more useful food ingredient.
Now a few examples of how we are attempting to achieve two of these
objectives which should contribute to reduced cost of production. We are
pursuing three approaches to incorporate more effective rate-reducing crown
rust resistance (likely more durable) into our potential varieties. We
are utilizing our recurrent selection (RS) methodologies on our RS population
improved for grain yield to also increase its rate-reducing rust resistance.
The poster at these meetings by Castell et al. describes the effort to
date. We are also crossing selected parents from that RS effort with lines
which are evaluated in our yield trials and which have been selected (after
crown rust inoculation at heading) because they lack an effective major
resistance gene, but do have noticeably less rust at maturity. We sometimes
refer to this kind of effort with our RS germ plasm as "mainstreaming".
Progeny from these kinds of crosses exposed to natural crown rust infection
at Palmerston North, New Zealand last winter had significantly less rust
than fully susceptible types.
Because these rate-reducing resistance genes are difficult to transfer
from one genotype to another, we are using two different approaches to
mark them molecularly to facilitate transfer. One approach will be to use
the model of DeKoeyer et al. and measure change in marker gene frequency
following cycles of RS for resistance and then test those markers whose
frequency changes form a pattern similar to the pattern of increase of
rate-reducing resistance.
A second approach is described in a poster by Chen et al. at these
meetings. Briefly, a set of recombinant inbred lines (RILs) from a cross
between 'Noble', a susceptible variety, and MN 841801, a line with a high
level of rate-reducing resistance, were inoculated in the field and the
greenhouse and then evaluated for rust development. These results will
be used to map the rate-reducing rust resistance genes in this population.
Regarding the increased competitiveness-with-weeds objective, we selected
contrasting sibs from several crosses growing in our field nurseries this
summer. These pairs will be crossed and progeny (beginning in the F2 generation
and ultimately RILs) will be evaluated for plant architecture that will
more quickly produce a closed canopy. These results will be used to identify
putative markers which, if actually linked to desired genes which produce
more competitive ability, can be used to select plants with the desired
phenotypes. Contrastingly, less competitive genotypes will also be selected
because they are useful as companion crops when attempting to establish
forage stands.
Eventually we may also cross the more competitive genotypes with some
from Brazil which appear to have some kind of allelopathic effects, at
least when grown preceding soybeans. Because of the subtleties of these
two traits, molecular markers may well be a necessity to make reasonable
progress.
In summary, oat breeders, geneticists and others who are interested
in advancing the oat crop should remain open to various possibilities of
increasing the fortunes of the crop. Thank you.
WHY DO FARMERS GROW OATS?
S. H. Weaver
The Quaker Oats Company, 617 West Main Street, Barrington, IL USA
60010
Farmers grow oats for money. The revenue that they gain may come in
a number of different ways. Oats may be grown as a cash crop for feed or
milling purposes or they may be grown as forage for livestock. In either
case, the oats have to generate sufficient returns or farmers will not
grow them. Farmers like to spread their risks and work loads by planting
several different crops. Oats are sometimes used in rotations and risk
management plans. Oats are also useful to break disease cycles in other
grains, such as take-all in wheat. Recently, oats have served as an alternative
in areas where scab is a problem in wheat and barley. Occasionally, the
situation is such that operating money is tight and farmers can and will
grow oats because they are perceived to be a low input crop. Low inputs
usually result in low income.
World oat production declined by 24% during the past ten years. The
decline was caused by lack of demand, low net returns per hectare, and
government programs. Relative to other feed grains, oats are expensive
and do not have the same feeding value. The demand for oats as food increased
slightly for the same period. The increase in food demand does not replace
the decrease in feed demand. The North American Free Trade Agreement (NAFTA),
General Agreement on Trade and Tariffs (GATT), the European Economic Community
and the Mercosur have affected world oat production and trade. Government
subsidies for raw commodities and transportation will decrease over the
next several years and that will be beneficial to oats in the short term.
However, the decline in oat production will continue into the future until
an economic equilibrium is met.
Assuming that oat prices remain constant or at least constant, relative
to other grains, how are cash farmers going to improve their net return
per hectare? They have to be more efficient and improve grain yields. At
constant prices, higher yields per hectare equate to higher net returns.
Consequently, oat breeders are working very hard to deliver higher yielding,
disease resistant varieties to farmers. From 1987 to 1997, the area planted
to oats in the US has declined by 70%. Government programs unfavorable
to oats, competition from other crops, Canadian rail subsidies, declining
feed demand, and diseases, especially crown rust, have contributed to the
sharp reduction in area planted to oats. During the same period, oat production
in the US has declined by almost 50%. The continual release of new, high
yielding varieties buffered the production decline in the US. Some of the
same forces that have caused sharp declines in the US are at work in Argentina.
Canadian oat production has increased slightly to feed the demand and
loss of production of oats in the US and South America. Transportation
subsidies and government controlled wheat prices have given Canadian producers
the incentive required to grow more oats. The 1997 North American oat production
was estimated at 6.2 million metric tons. Projections for 1998 are 6.3
million metric tons. Price stability may indicate that the supply is remaining
much higher than the demand. When the supply and demand come closer together,
more price volatility might be expected.
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