| US 7,476,507 B2 | ||
| Assay for porcine circovirus production | ||
| Patrice Allibert, Athens, Ga. (US); Lionel Pierre Christian Cupillard, Bougoin Jallieu (France); and Jean Reyes, Lyons (France) | ||
| Assigned to Merial Limited, Duluth, Ga. (US) | ||
| Filed on Apr. 11, 2008, as Appl. No. 12/101,512. | ||
| Application 12/101512 is a division of application No. 11/403497, filed on Apr. 13, 2006, granted, now 7,358,075. | ||
| Claims priority of provisional application 60/670892, filed on Apr. 13, 2005. | ||
| Prior Publication US 2008/0241821 A1, Oct. 02, 2008 | ||
| Int. Cl. C12Q 1/68 (2006.01) | ||
| U.S. Cl. 435—6 | 4 Claims |
| 1. A method for monitoring circovirus production in a host cell culture infected with circovirus, the method comprising:
a) monitoring expression of ORF1 and/or ORF2 in adherent and non-adherent host cells using a fluorescent antibody cell sorting
(FACS)-based method; and
b) plotting expression of ORF1 and/or ORF2 over time; thereby monitoring circovirus production in the host cell culture.
|