Connor R, Mohri H, Daar E, Ho DD; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 156 (abstract no. W.A.1256).
The Aaron Diamond AIDS Research Center, New York, New York, USA
OBJECTIVE: To evaluate the functional properties and molecular heterogeneity of a series of biologic clones of HIV-1 simultaneously isolated from an individual. METHODS: Biologic clones of HIV-1 were obtained by limiting dilution culture of PBMC from HIV-1 seropositive individuals. A total of 27 HIV-1 clones from three different patients (MD, JSH and N70) were evaluated for replication kinetics, host cell range and cytopathicity in primary cultures of PBMC and macrophages, and in several T cell lines (H9, MOLT-4, CEM, HPB-ALL). RESULTS: All HIV-1 clones isolated by this method were infectious in PBMC cultures. Clones from patient JSH showed markedly different replication kinetics in PBMC and macrophage cultures. Several clones replicated rapidly to high titer, while others were characterized by slow infection kinetics. HIV-1 clones from JSH were generally able to infect T cell lines, although some distinct differences were observed depending on the clone and the target cell used. All HIV-1 clones from JSH were capable of inducing syncytia formation. By contrast, all HIV-1 clones from patient MD had similar patterns of replication in PBMC and macrophage cultures, and were unable to infect T-cell lines or induce syncytia formation. Currently 10 infectious clones from patient N70 are under similar evaluation. Several HIV-1 clones from patient JSH with divergent biological activities were selected for further molecular characterization, including the determination of the primary sequence of env. CONCLUSIONS: This approach provides an effective means for the isolation and characterization of infectious HIV-1 clones, and may be useful in elucidating the structural determinants responsible for the observed biological heterogeneity.
Publication Types:
Keywords:
- Base Sequence
- Cell Line
- Clone Cells
- Genes, env
- HIV-1
- Humans
- Macrophages
- T-Lymphocytes
- genetics
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102192464
From Meeting Abstracts