BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract

RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice:  statistical significance is estimated under the assumption that the
equivalent of one entire reading frame in the query sequence codes for protein
and that significant alignments will involve only coding reading frames.

Query=  A01A12_CONSENSUS
        (586 letters)
  Translating both strands of query sequence in all 6 reading frames

Database:  nr
           625,274 sequences; 197,782,623 total letters.


     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 2 Sequences     : less than 2 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 573  94 |===============================================
   6310 479  79 |=======================================
   3980 400 113 |========================================================
   2510 287  85 |==========================================
   1580 202  55 |===========================
   1000 147  38 |===================
    631 109  36 |==================
    398  73  16 |========
    251  57  20 |==========
    158  37  10 |=====
    100  27   7 |===
   63.1  20   8 |====
   39.8  12   4 |==
   25.1   8   1 |:
   15.8   7   1 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 6  <<<<<<<<<<<<<<<<<
   10.0   6   1 |:
   6.31   5   1 |:
   3.98   4   0 |
   2.51   4   3 |=


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|8843774|dbj|BAA97322.1|(AB020754) gene_id:MYN8.6~s... +1   512  5.4e-54   2
gi|7469320|pir||S75332fibrillin - Synechocystis sp. (... +1    84  0.82      1
gi|5020103|gb|AAD38023.1|AF148219_1(AF148219) fibrill... +1    83  0.87      1
gi|7489044|pir||T07125plastid-lipid-associated protei... +1    80  0.87      1
gi|6018307|gb|AAF01813.1|AF187532_9(AF187532) nogalon... +1    76  0.991     1
gi|4139097|gb|AAD03693.1|(AF084554) fibrillin [Brassi... +1    80  0.9992    1



Locally-aligned regions (HSPs) with respect to query sequence:

Locus_ID                Frame 3 Hits
gi|8843774             |                                    ______________
                        __________________________________________________
Query sequence:        |            |            |            |           | 196
                       0           50          100          150


Locus_ID                Frame 1 Hits
gi|8843774             |_________________________________                 
gi|7469320             |              _________                           
gi|5020103             |         ______________                           
gi|7489044             |           ___________________                    
gi|6018307             |                 _________________                
gi|4139097             |          _________________                       
                        __________________________________________________
Query sequence:        |            |            |            |           | 196
                       0           50          100          150


Use the  and  icons to retrieve links to Entrez:
E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).

Use the  icon (if present) to retrieve links to the Sequence Retrieval System (SRS).

Use the  icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .

Use the  icon (if present) to retrieve links to the Protein Data Bank database.


 >gi|8843774|dbj|BAA97322.1|  (AB020754) gene_id:MYN8.6~similar to unknown
            protein~sp|P29618 [Arabidopsis thaliana]
            Length = 670

Frame  3 hits (HSPs):                                           _____     
Frame  1 hits (HSPs):                                ___________          
                        __________________________________________________
Database sequence:     |           |          |          |          |     | 670
                       0         150        300        450        600

  Plus Strand HSPs:

 Score = 512 (180.2 bits), Expect = 5.4e-54, Sum P(2) = 5.4e-54
 Identities = 92/131 (70%), Positives = 109/131 (83%), Frame = +1

Query:     1 LRHPFLCGPRWRVVPSMDIIRWGLGSTAMRIREKYIYRQPQQSRLAHFIDLMXMLNPHPQ 180
             L+HPFLCGPRWRV PSMDIIRWGLGSTA++I E+YIYR PQ+ RLAHFI LM MLNP+P+
Sbjct:   394 LKHPFLCGPRWRVAPSMDIIRWGLGSTAVKISEEYIYRMPQRQRLAHFIGLMEMLNPYPK 453

Query:   181 PRNWLELLPGKWRLLYSTGKHVGLTLRQPPLRVLVSDVHLTVTRESKLKDN--LSFGSDI 354
             P  WLELLPG+WRLLYSTGKH+GLTLRQP  R L+ +VHLT+TR S+  +N  LSF SDI
Sbjct:   454 PNCWLELLPGRWRLLYSTGKHIGLTLRQPSTRALIGNVHLTITRASESINNTSLSFTSDI 513

Query:   355 GFSVMIGQDWP 387
              F+ +  +DWP
Sbjct:   514 RFTAITSKDWP 524

 Score = 73 (25.7 bits), Expect = 5.4e-54, Sum P(2) = 5.4e-54
 Identities = 21/54 (38%), Positives = 25/54 (46%), Frame = +3

Query:   429 SLFTLXSGXRLYLKQDXTTX-KFFFGPSTMFEALLXNLRAXNGKRY-PFXGFPS 584
             S F L +G RLYLK++     KF  G     E L   L     K+  PF  FPS
Sbjct:   538 SQFRLIAGKRLYLKEEKKNIGKFSMGEPDAEEGLAEKLETEKWKKVVPFKEFPS 591


 >gi|7469320|pir||S75332  fibrillin - Synechocystis sp. (strain PCC 6803)
            >gi|1652323|dbj|BAA17246.1| (D90904) fibrillin [Synechocystis sp.]
            Length = 202

Frame  1 hits (HSPs):              _________                              
                        __________________________________________________
Database sequence:     |            |           |           |            || 202
                       0           50         100         150          200

  Plus Strand HSPs:

 Score = 84 (29.6 bits), Expect = 1.7, P = 0.82
 Identities = 17/36 (47%), Positives = 23/36 (63%), Frame = +1

Query:   166 NPHPQPRNWLELLPGKWRLLYSTGKHVGLTLRQPPL 273
             NPHP+P     LL G WRLLY++ + + L L + PL
Sbjct:    47 NPHPKPLQEKNLLDGNWRLLYTSSQSI-LGLNRLPL 81


 >gi|5020103|gb|AAD38023.1|AF148219_1  (AF148219) fibrillin [Nostoc sp. PCC 8009]
            Length = 194

Frame  1 hits (HSPs):        ______________                               
                        __________________________________________________
Database sequence:     |            |            |            |           | 194
                       0           50          100          150

  Plus Strand HSPs:

 Score = 83 (29.2 bits), Expect = 2.1, P = 0.87
 Identities = 23/52 (44%), Positives = 29/52 (55%), Frame = +1

Query:   115 QPQQSRLAHFIDLMXMLNPHPQPRNWLELLPGKWRLLYSTGKHVGLTLRQPP 270
             Q +Q+ LA    L   LNP P+P     LL G WRLLY+T K + L L + P
Sbjct:    23 QQKQAILAAIARLED-LNPTPRPVEATNLLDGNWRLLYTTSKAL-LNLDRVP 72


 >gi|7489044|pir||T07125  plastid-lipid-associated protein PAP - tomato
            (fragment) >gi|2632090|emb|CAA75658.1| (Y15490)
            Plastid-lipid-Associated Protein [Lycopersicon esculentum]
            Length = 146

Frame  1 hits (HSPs):                       ____________________________  
                        __________________________________________________
Database sequence:     |                |                |                | 146
                       0               50              100

  Plus Strand HSPs:

 Score = 80 (28.2 bits), Expect = 2.1, P = 0.87
 Identities = 25/78 (32%), Positives = 40/78 (51%), Frame = +1

Query:   136 AHFIDLMXML---NPHPQPRNWLELLPGKWRLLYSTGKHVGLTL-RQPPLRVLVSDVHLT 303
             A  ++L+  L   NP+P P   L LL GKW L Y++   +  +L R   L V V ++  T
Sbjct:    62 AEIVELITQLESKNPNPAPTEALTLLNGKWILAYTSFSGLFPSLSRGNLLLVRVEEISQT 121

Query:   304 VTRES-KLKDNLSFGSDI 354
             +  ES  ++D+  F   +
Sbjct:   122 IDSESFTVQDSAVFAGPL 139


 >gi|6018307|gb|AAF01813.1|AF187532_9  (AF187532) nogalonic acid methyl ester
            cyclase [Streptomyces nogalater]
            Length = 134

Frame  1 hits (HSPs):                          __________________________ 
                        __________________________________________________
Database sequence:     |                  |                 |             | 134
                       0                 50               100

  Plus Strand HSPs:

 Score = 76 (26.8 bits), Expect = 4.7, P = 0.99
 Identities = 20/69 (28%), Positives = 29/69 (42%), Frame = +1

Query:   208 GKWRLLYST--GKHVGLTLRQPPL--RVLVSDVHLTVTRESKLKDNLSFGSDIGFSVMIG 375
             G W   Y    G+HVG  +  PP   R     VHL    + K++D+  +    G    +G
Sbjct:    64 GPWVKAYLVLYGRHVGRLVGMPPTDRRFSGEQVHLMRIVDGKIRDHRDWPDFQGTLRQLG 123

Query:   376 QDWPP*QSW 402
               WP  + W
Sbjct:   124 DPWPDDEGW 132


 >gi|4139097|gb|AAD03693.1|  (AF084554) fibrillin [Brassica napus]
            Length = 237

Frame  1 hits (HSPs):          ______________                             
                        __________________________________________________
Database sequence:     |          |         |          |         |        | 237
                       0         50       100        150       200

  Plus Strand HSPs:

 Score = 80 (28.2 bits), Expect = 7.1, P = 1.0
 Identities = 22/66 (33%), Positives = 36/66 (54%), Frame = +1

Query:   124 QSRLAHFIDLMXMLNPHPQPRNWLELLPGKWRLLYST--GKHVGLTLRQPPLRVLVSDVH 297
             ++ ++  I  +   NP+P P   L LL GKW L+Y++  G    L+ R  PL V V ++ 
Sbjct:    35 RAEISELITQLESKNPNPAPNEALFLLNGKWILVYTSFVGLFPLLSRRISPL-VKVDEIS 93

Query:   298 LTVTRES 318
              T+  +S
Sbjct:    94 QTIDSDS 100


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.95

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.336   0.144   0.494  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.353   0.156   0.580  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.338   0.151   0.532  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.333   0.145   0.453  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.347   0.151   0.570  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.351   0.158   0.586  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      194       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   +2      0      195       184       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   +1      0      195       184       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -1      0      195       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -2      0      195       185       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -3      0      194       182       10.  76 3  12 22  0.12    34
                                                    31  0.12    37


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  6
  No. of states in DFA:  600 (59 KB)
  Total size of DFA:  225 KB (256 KB)
  Time to generate neighborhood:  0.01u 0.01s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  187.35u 1.07s 188.42t  Elapsed: 00:00:35
  Total cpu time:  187.38u 1.10s 188.48t  Elapsed: 00:00:35
  Start:  Mon Oct  1 16:06:57 2001   End:  Mon Oct  1 16:07:32 2001


Annotated Domains Database:   March 14, 2000 

Release Date:  March 14, 2000