UM0061

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

194 ESTs from this library were grouped into 129 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
18	92784		Hs.284244	Fibroblast growth factor 2 (basic).
12	61856		Hs.713943	Transcribed locus, strongly similar to NP_056962.2 ornithine decarboxylase antizyme inhibitor [Homo sapiens].
9	46392		Hs.449909	Ribosomal protein SA.
4	20619		Hs.533624	H3 histone, family 3A.
4	20619		Hs.459940	Lipopolysaccharide-induced TNF factor.
3	15464		Hs.80545	Ribosomal protein L37.
3	15464		Hs.473838	Proteasome (prosome, macropain) assembly chaperone 1.
3	15464		Hs.2057	Uridine monophosphate synthetase.
2	10309		Hs.718407	Homeodomain interacting protein kinase 2.
2	10309		Hs.713967	Low density lipoprotein receptor.
2	10309		Hs.654404	Major histocompatibility complex, class I, C.
2	10309		Hs.645643	Transcribed locus, strongly similar to XP_867599.1 PREDICTED: similar to 40S ribosomal protein SA (p40) (34/67 kDa laminin receptor) (Colon carcinoma laminin-binding protein) (NEM/1CHD4) (Multidrug resistance-associated protein MGr1-Ag) isoform 3 [Canis familiaris].
2	10309		Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
2	10309		Hs.533712	RNA binding motif protein 4.
2	10309		Hs.529303	Actin related protein 2/3 complex, subunit 2, 34kDa.
2	10309		Hs.524390	Tubulin, alpha 1b.
2	10309		Hs.514581	Actin, gamma 1.
2	10309		Hs.510635	Immunoglobulin heavy constant gamma 1 (G1m marker).
2	10309		Hs.497118	Cytochrome c oxidase subunit VIa polypeptide 1.
2	10309		Hs.458917	Secretory carrier membrane protein 2.
2	10309		Hs.444931	Mediator complex subunit 17.
2	10309		Hs.380897	AKT interacting protein.
2	10309		Hs.189772	Chaperonin containing TCP1, subunit 2 (beta).
2	10309		Hs.153961	ARP1 actin-related protein 1 homolog A, centractin alpha (yeast).
2	10309		Hs.150107	Baculoviral IAP repeat-containing 6.
1	5155		Hs.76277	Receptor accessory protein 6.
1	5155		Hs.718441	LUC7-like 2 (S. cerevisiae).
1	5155		Hs.715537	Transducin (beta)-like 1 X-linked receptor 1.
1	5155		Hs.715064	Transcribed locus.
1	5155		Hs.714700	WW domain containing adaptor with coiled-coil.
1	5155		Hs.713764	Transcribed locus, strongly similar to XP_777977.1 hypothetical protein CNBA4460 [Cryptococcus neoformans var. neoformans B-3501A].
1	5155		Hs.710953	Transcribed locus, strongly similar to NP_000602.1 CD59 antigen preproprotein [Homo sapiens].
1	5155		Hs.709235	Keratin 6B.
1	5155		Hs.708359	Transcribed locus, strongly similar to NP_001755.1 CD1B antigen precursor [Homo sapiens].
1	5155		Hs.708294	Transcribed locus.
1	5155		Hs.702563	Transcribed locus.
1	5155		Hs.701182	Transcribed locus.
1	5155		Hs.699822	Immunoglobulin superfamily containing leucine-rich repeat.
1	5155		Hs.698104	Transcribed locus.
1	5155		Hs.68864	Lipase, member H.
1	5155		Hs.669058	CDNA FLJ14364 fis, clone HEMBA1000918.
1	5155		Hs.654949	PEST proteolytic signal containing nuclear protein.
1	5155		Hs.654600	Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor).
1	5155		Hs.650962	CDNA FLJ27020 fis, clone SLV06144.
1	5155		Hs.647094	Fas-activated serine/threonine kinase.
1	5155		Hs.644306	Tropomyosin 3.
1	5155		Hs.643498	Chromosome 6 open reading frame 106.
1	5155		Hs.640434	Cytoskeleton associated protein 2.
1	5155		Hs.632603	Transcribed locus, strongly similar to NP_990627.1 H3 histone, family 3B (H3.3B) [Gallus gallus].
1	5155		Hs.629638	Deoxyribonuclease I.
1	5155		Hs.621461	Interleukin 23, alpha subunit p19.
1	5155		Hs.614588	Transcribed locus, strongly similar to NP_001013145.1 transgelin 2 [Rattus norvegicus].
1	5155		Hs.604915	Transcribed locus.
1	5155		Hs.593499	Transcribed locus.
1	5155		Hs.593318	Transcribed locus, strongly similar to NP_000055.2 complement component 3 precursor [Homo sapiens].
1	5155		Hs.591663	Eomesodermin homolog (Xenopus laevis).
1	5155		Hs.591610	Oligonucleotide/oligosaccharide-binding fold containing 2A.
1	5155		Hs.567943	40S ribosomal protein SA pseudogene.
1	5155		Hs.546259	H3 histone, family 3A.
1	5155		Hs.533771	Jumonji domain containing 8.
1	5155		Hs.532359	Ribosomal protein L5.
1	5155		Hs.531713	COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis).
1	5155		Hs.530823	COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis).
1	5155		Hs.524590	RAB21, member RAS oncogene family.
1	5155		Hs.523238	Nucleolar and coiled-body phosphoprotein 1.
1	5155		Hs.521008	STEAP family member 4.
1	5155		Hs.518774	Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase.
1	5155		Hs.518099	Chromosome 3 open reading frame 38.
1	5155		Hs.517546	Oxysterol binding protein 2.
1	5155		Hs.517216	Phosphoprotein enriched in astrocytes 15.
1	5155		Hs.515247	Janus kinase 3 (a protein tyrosine kinase, leukocyte).
1	5155		Hs.514821	Chemokine (C-C motif) ligand 5.
1	5155		Hs.514465	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d.
1	5155		Hs.513851	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide.
1	5155		Hs.509226	FK506 binding protein 3, 25kDa.
1	5155		Hs.508848	Heterogeneous nuclear ribonucleoprotein C (C1/C2).
1	5155		Hs.50425	Prostaglandin E synthase 3 (cytosolic).
1	5155		Hs.502875	V-rel reticuloendotheliosis viral oncogene homolog A (avian).
1	5155		Hs.500598	TBC1 domain family, member 12.
1	5155		Hs.497599	Tryptophanyl-tRNA synthetase.
1	5155		Hs.49427	GEM interacting protein.
1	5155		Hs.494173	Annexin A1.
1	5155		Hs.484241	Clathrin, light chain (Lcb).
1	5155		Hs.481571	Ubiquinol-cytochrome c reductase hinge protein.
1	5155		Hs.471104	NOP58 ribonucleoprotein homolog (yeast).
1	5155		Hs.471040	Chromosome 2 open reading frame 69.
1	5155		Hs.449585	Immunoglobulin lambda locus.
1	5155		Hs.446374	Prefoldin subunit 6.
1	5155		Hs.445977	General transcription factor IIIA.
1	5155		Hs.440475	Succinate dehydrogenase complex, subunit A, flavoprotein (Fp).
1	5155		Hs.439480	RNA binding motif protein 6.
1	5155		Hs.439463	AE binding protein 1.
1	5155		Hs.436578	Polymerase (RNA) II (DNA directed) polypeptide F.
1	5155		Hs.436568	CD74 molecule, major histocompatibility complex, class II invariant chain.
1	5155		Hs.431317	Golgi reassembly stacking protein 2, 55kDa.
1	5155		Hs.419640	Parkinson disease (autosomal recessive, early onset) 7.
1	5155		Hs.407368	Transcribed locus.
1	5155		Hs.402752	TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa.
1	5155		Hs.38972	Tetraspanin 1.
1	5155		Hs.375217	Ring finger protein 31.

Source: NCBI UniGene

EST Sequences

296 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• uterus
• adult
• bulk

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).