R = Retrieve links to Related sequences (neighbors).
Use the 
icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the 
icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the 
icon (if present) to retrieve links to the Protein Data Bank database.
WARNING: Descriptions of 1 database sequences were not reported due to the
limiting value of parameter V = 50.
>gi|9294296|dbj|BAB02198.1| (AP001298) contains similarity to alpha
galactosidase~gene_id:F20C19.10 [Arabidopsis thaliana]
Length = 676
Frame 2 hits (HSPs): ___________
Frame 1 hits (HSPs): __
__________________________________________________
Database sequence: | | | | | | 676
0 150 300 450 600
Plus Strand HSPs:
Score = 551 (194.0 bits), Expect = 2.1e-54, Sum P(2) = 2.1e-54
Identities = 103/137 (75%), Positives = 117/137 (85%), Frame = +2
Query: 17 SLFILLFLCSESVSSQNV-SESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRL 193
++F ++F + S+ S ++ + S QQ AS PPRGWNSYDSFCWTISE EFLQSAEIVS+RL
Sbjct: 15 TVFFIIF--NLSIFSLSIEARSRQQHASFPPRGWNSYDSFCWTISEAEFLQSAEIVSKRL 72
Query: 194 HDHGYEYVVVDYLWYRRKVEGAYHDSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQ 373
HGY+YVVVDYLWYR+KVEGAY DSLGFDVIDEWGR+ PDPGRWPSS GKGFTEVA +
Sbjct: 73 LPHGYQYVVVDYLWYRKKVEGAYVDSLGFDVIDEWGRLHPDPGRWPSSRGGKGFTEVAEK 132
Query: 374 VHSMGLKFGIHVMRGIST 427
VH MGLKFGIHVM GIST
Sbjct: 133 VHRMGLKFGIHVMGGIST 150
Score = 38 (13.4 bits), Expect = 2.1e-54, Sum P(2) = 2.1e-54
Identities = 8/9 (88%), Positives = 8/9 (88%), Frame = +1
Query: 427 IHVMRGIST 453
IHVM GIST
Sbjct: 142 IHVMGGIST 150
>gi|10174488|dbj|BAB05589.1| (AP001513) BH1870~unknown conserved protein in
others [Bacillus halodurans]
Length = 432
Frame 2 hits (HSPs): ______________
__________________________________________________
Database sequence: | | | | 432
0 150 300
Plus Strand HSPs:
Score = 273 (96.1 bits), Expect = 1.6e-22, P = 1.6e-22
Identities = 48/116 (41%), Positives = 76/116 (65%), Frame = +2
Query: 74 ESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVE 253
E + A PP GWNS+D + +++EEE L +AE ++ L +G+EY+VVD WY
Sbjct: 2 EVNRLSALTPPMGWNSWDCYGASVTEEEVLGNAEYMANHLKKYGWEYIVVDIQWYEPTAN 61
Query: 254 GAYHDSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGI 421
+ ++ +DE+GR++P R+PS++NG GF +++ +H +GLKFGIH+MRGI
Sbjct: 62 SSAYNPFAPLCMDEYGRLLPATNRFPSAKNGAGFKPLSDAIHDLGLKFGIHIMRGI 117
>gi|4091877|gb|AAC99325.1| (AF061331) alpha galactosidase precursor
[Saccharopolyspora erythraea]
Length = 428
Frame 2 hits (HSPs): ___________
__________________________________________________
Database sequence: | | | | 428
0 150 300
Plus Strand HSPs:
Score = 111 (39.1 bits), Expect = 5.5e-10, Sum P(2) = 5.5e-10
Identities = 22/50 (44%), Positives = 30/50 (60%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLWY 238
A PP GWNS++SF I E +A+ +V + D GY+YVVVD W+
Sbjct: 44 ARTPPMGWNSWNSFGCDIDERLIRDTADALVGSGMRDAGYQYVVVDDCWF 93
Score = 71 (25.0 bits), Expect = 5.5e-10, Sum P(2) = 5.5e-10
Identities = 20/47 (42%), Positives = 26/47 (55%), Frame = +2
Query: 266 DSLGFDVI-DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIH 406
D FD D G + +P R+PS G +A+ VHS GLKFGI+
Sbjct: 89 DDCWFDPQRDPQGNLRANPERFPS-----GIRALADYVHSRGLKFGIY 131
>gi|7706787|gb|AAB35252.2| (S79440) alpha-galactosidase [Mortierella vinacea]
Length = 417
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 417
0 150 300
Plus Strand HSPs:
Score = 110 (38.7 bits), Expect = 1.0e-09, Sum P(2) = 1.0e-09
Identities = 24/63 (38%), Positives = 35/63 (55%), Frame = +2
Query: 68 VSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLWYRR 244
V S A P GWN+++ + I E+ L +A+ IVS L D+GY YVV+D W +
Sbjct: 18 VLASNNGLAITPQMGWNTWNKYGCNIDEQLILDAAKAIVSSGLKDYGYNYVVIDDCWQKN 77
Query: 245 KVE 253
+ E
Sbjct: 78 ERE 80
Score = 69 (24.3 bits), Expect = 1.0e-09, Sum P(2) = 1.0e-09
Identities = 14/41 (34%), Positives = 24/41 (58%), Frame = +2
Query: 305 MVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
++PDP ++P +G + + +H+MGLK GI+ G T
Sbjct: 85 LLPDPTKFP-----RGMKPLVDDIHAMGLKVGIYSSAGTLT 120
>gi|5326775|emb|CAB46229.1| (Y18586) alpha-galactosidase [Aspergillus niger]
Length = 443
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 443
0 150 300
Plus Strand HSPs:
Score = 150 (52.8 bits), Expect = 6.8e-09, P = 6.8e-09
Identities = 36/109 (33%), Positives = 63/109 (57%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSA-EIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDSLG 277
P GWNS++++ I ++ + +A E+V+ L D GYEY+ +D W V+ + +
Sbjct: 27 PALGWNSWNAYSCDIDADKIVTAANEVVNLGLKDLGYEYINIDDCW---SVKSGRNTTTK 83
Query: 278 FDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
R++PDP ++P+ G + VA+QVH++GLK GI+ G++T
Sbjct: 84 --------RIIPDPDKFPN-----GISGVADQVHALGLKLGIYSSAGLTT 120
>gi|7435458|pir||JC5558 alpha-galactosidase (EC 3.2.1.22) II precursor -
Mortierella vinacea >gi|3777481|dbj|BAA33931.1| (AB018691)
alpha-galactosidase [Mortierella vinacea]
Length = 396
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 396
0 150 300
Plus Strand HSPs:
Score = 140 (49.3 bits), Expect = 6.8e-08, P = 6.8e-08
Identities = 37/112 (33%), Positives = 60/112 (53%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLWYRRKVEGAYHD 268
A P GWNS++ + ++E + +A +VS L D GY Y+ +D W + H
Sbjct: 27 AKTPQMGWNSWNKYQCNVNETVIINTANAMVSSGLKDLGYHYINIDDCW-------SLHQ 79
Query: 269 SLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D + R+ PDP ++P+ G + VA++VH++GLKFGI+ G +T
Sbjct: 80 R---DNTTQ--RIAPDPTKFPN-----GISGVASKVHALGLKFGIYSDAGTNT 122
>gi|9188530|dbj|BAA99555.1| (AB030209) alpha-galactosidase [Zygosaccharomyces
mrakii]
Length = 470
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | | 470
0 150 300 450
Plus Strand HSPs:
Score = 109 (38.4 bits), Expect = 1.1e-07, Sum P(2) = 1.1e-07
Identities = 25/75 (33%), Positives = 43/75 (57%), Frame = +2
Query: 20 LFILLFLCSESV--SSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQR- 190
+F LL L S ++ ++ VS S P GWN++++F ++E+ L +A+ +S+
Sbjct: 1 MFSLLLLTSTALVETALGVSPSYNGLGLTPQMGWNNWNTFACNVTEQLLLGTADRISELG 60
Query: 191 LHDHGYEYVVVDYLW 235
L D GY YV++D W
Sbjct: 61 LKDVGYNYVILDDCW 75
Score = 52 (18.3 bits), Expect = 1.1e-07, Sum P(2) = 1.1e-07
Identities = 13/40 (32%), Positives = 20/40 (50%), Frame = +2
Query: 299 GRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
G +VPD ++P G VA+ +H L FG++ G
Sbjct: 83 GSLVPDLNKFPH-----GMKYVADHLHDQDLLFGMYSSAG 117
>gi|1170911|sp|P41946|MEL5_YEAST ALPHA-GALACTOSIDASE 5 PRECURSOR (MELIBIASE 5)
(ALPHA-D-GALACTOSIDE GALACTOHYDROLASE 5) >gi|1084808|pir||S50311
alpha-galactosidase (EC 3.2.1.22) MEL5 - yeast (Saccharomyces
cerevisiae) (strain CBS4411) >gi|547464|emb|CAA85740.1| (Z37511)
alpha-galactosidase [Saccharomyces cerevisiae]
Length = 471
Frame 2 hits (HSPs): _____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | 471
0 150 300 450
__________________
Annotated Domains:
BLOCKS BL00512A: Alpha-galactosidase proteins. 24..44
BLOCKS BL00512B: Alpha-galactosidase proteins. 65..75
BLOCKS BL00512C: Alpha-galactosidase proteins. 79..121
BLOCKS BL00512D: Alpha-galactosidase proteins. 131..153
Entrez glycosylation site: POTENTIAL. 105
Entrez glycosylation site: POTENTIAL. 175
Entrez glycosylation site: POTENTIAL. 270
Entrez glycosylation site: POTENTIAL. 370
Entrez glycosylation site: POTENTIAL. 403
Entrez glycosylation site: POTENTIAL. 422
Entrez glycosylation site: POTENTIAL. 435
Entrez glycosylation site: POTENTIAL. 454
PRINTS 1: Glycosyl hydrolase family 24..43
PRINTS 2: Glycosyl hydrolase family 60..75
PRINTS 3: Glycosyl hydrolase family 100..121
PRINTS 4: Glycosyl hydrolase family 134..151
PRINTS MELIBIASE1: Melibiase motif I - 2 150..165
PRINTS 5: Glycosyl hydrolase family 166..184
PRINTS MELIBIASE2: Melibiase motif II - 2 213..232
PRINTS 6: Glycosyl hydrolase family 253..272
PRINTS 7: Glycosyl hydrolase family 274..295
PRINTS MELIBIASE3: Melibiase motif III - 2 326..342
PRINTS MELIBIASE4: Melibiase motif IV - 2 351..367
PRINTS MELIBIASE5: Melibiase motif V - 2 374..397
PRINTS MELIBIASE6: Melibiase motif VI - 2 400..422
PRINTS MELIBIASE7: Melibiase motif VII - 2 428..444
PRINTS MELIBIASE8: Melibiase motif VIII - 2 449..469
PRODOM PD018118: MEL1(1) MEL2(1) MEL6(1) 1..22
PRODOM PD002572: AGAL(6) O13295(2) O94221(2) 24..425
PRODOM PD008767: 427..470
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 65..80
__________________
Plus Strand HSPs:
Score = 109 (38.4 bits), Expect = 2.8e-07, Sum P(2) = 2.8e-07
Identities = 30/91 (32%), Positives = 45/91 (49%), Frame = +2
Query: 23 FILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQR-LHD 199
F L C+ VS S P GW+S+++F +SE+ L +A+ +S L D
Sbjct: 4 FYFLTACTTLKGVFGVSPSYNGLGLTPQMGWDSWNTFACDVSEQLLLDTADRISDLGLKD 63
Query: 200 HGYEYVVVDYLWYRRKVEGAYHDSLGFDVIDE 295
GY+YV++D W + DS GF V D+
Sbjct: 64 MGYKYVILDDCW------SSGRDSDGFLVADK 89
Score = 48 (16.9 bits), Expect = 2.8e-07, Sum P(2) = 2.8e-07
Identities = 14/43 (32%), Positives = 22/43 (51%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
D G +V D ++P NG G VA+ +H+ FG++ G
Sbjct: 80 DSDGFLVADKHKFP---NGMG--HVADHLHNNSFLFGMYSSAG 117
>gi|3821271|emb|CAA08915.1| (AJ009956) alpha-galactosidase 1 [Penicillium
simplicissimum]
Length = 435
Frame 2 hits (HSPs): ___________
__________________________________________________
Database sequence: | | | | 435
0 150 300
Plus Strand HSPs:
Score = 133 (46.8 bits), Expect = 4.6e-07, P = 4.6e-07
Identities = 36/110 (32%), Positives = 58/110 (52%), Frame = +2
Query: 98 IPPRGWNSYDSFCWTISEEEFLQSA-EIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDSL 274
+P GWNS+++F + + + +A E+V L D GYEYV +D W V+ + +
Sbjct: 28 LPALGWNSWNAFGCDVDSTKIMTAANEMVHLGLKDLGYEYVNIDDCW---SVKNTRNSTT 84
Query: 275 GFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
R++PD ++P G + VA+QVH +GLK GI+ G +T
Sbjct: 85 Q--------RIIPDTQKFPD-----GISGVADQVHQLGLKIGIYSSAGETT 122
>gi|7492381|pir||T39118 probable alpha-galactosidase - fission yeast
(Schizosaccharomyces pombe) >gi|6224598|emb|CAB60017.1| (AL132779)
putative alpha-galactosidase [Schizosaccharomyces pombe]
Length = 436
Frame 2 hits (HSPs): ______________
__________________________________________________
Database sequence: | | | | 436
0 150 300
Plus Strand HSPs:
Score = 131 (46.1 bits), Expect = 7.6e-07, P = 7.6e-07
Identities = 40/138 (28%), Positives = 66/138 (47%), Frame = +2
Query: 5 LSSISLFILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVS 184
+S ++ F L+FL + +V S P GWNS++ + I E L +A+ +
Sbjct: 4 ISFLNCFFLVFLF---LFFSDVHGSYNGLGLKPQMGWNSWNKYACDIDESIILNNAKAIK 60
Query: 185 QR-LHDHGYEYVVVDYLWYRRKVEGAYHDSLGFDVIDEWGRMVPDPGRWPSSENGKGFTE 361
+ L D GYEY+V+D W + + GR+ +P ++P NG G
Sbjct: 61 EEGLLDLGYEYIVMDDCWSKHERNATT------------GRLEANPDKFP---NGIG--S 103
Query: 362 VANQVHSMGLKFGIHVMRG 418
+A ++H MG KFG++ G
Sbjct: 104 MAKKLHDMGFKFGMYSSAG 122
>gi|2570148|dbj|BAA22992.1| (AB008367) alpha-galactosidase [Penicillium
purporogenum]
Length = 439
Frame 2 hits (HSPs): ___________
__________________________________________________
Database sequence: | | | | 439
0 150 300
Plus Strand HSPs:
Score = 125 (44.0 bits), Expect = 3.4e-06, P = 3.4e-06
Identities = 34/110 (30%), Positives = 58/110 (52%), Frame = +2
Query: 98 IPPRGWNSYDSFCWTISEEEFLQSA-EIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDSL 274
+P GWNS++++ ++E + + +A ++ + L GY+YV +D W V+
Sbjct: 29 LPALGWNSWNAYGCNVNETKIVTAATKLNTTGLQALGYQYVNIDDCW---SVKS------ 79
Query: 275 GFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
G D + R++P+P +P+ NG A QVH +GLK GI+ G T
Sbjct: 80 GRDNVTN--RIIPNPDTFPNGING-----TAQQVHDLGLKIGIYSSAGYET 123
>gi|113501|sp|P04824|MEL1_YEAST ALPHA-GALACTOSIDASE 1 PRECURSOR (MELIBIASE 1)
(ALPHA-D-GALACTOSIDE GALACTOHYDROLASE 1) >gi|67494|pir||GBBYAG
alpha-galactosidase (EC 3.2.1.22) MEL1 precursor - yeast
(Saccharomyces cerevisiae) >gi|3920|emb|CAA26888.1| (X03102)
precursor [Saccharomyces cerevisiae] >gi|171926|gb|AAA34770.1|
(M10604) pre-alpha galactosidase (melibiase) [Saccharomyces
pastorianus] >gi|5257486|gb|AAD41358.1|AF152092_1 (AF152092)
alpha-galactosidase [Cloning vector pGB-MEL1]
Length = 471
Frame 2 hits (HSPs): _____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | 471
0 150 300 450
__________________
Annotated Domains:
BLOCKS BL00512A: Alpha-galactosidase proteins. 24..44
BLOCKS BL00512B: Alpha-galactosidase proteins. 65..75
BLOCKS BL00512C: Alpha-galactosidase proteins. 79..121
BLOCKS BL00512D: Alpha-galactosidase proteins. 131..153
DOMO DM01493: ALPHA-GALACTOSIDASE 11..425
Entrez glycosylation site: POTENTIAL. 105
Entrez glycosylation site: POTENTIAL. 175
Entrez glycosylation site: POTENTIAL. 270
Entrez glycosylation site: POTENTIAL. 370
Entrez glycosylation site: POTENTIAL. 403
Entrez glycosylation site: POTENTIAL. 413
Entrez glycosylation site: POTENTIAL. 422
Entrez glycosylation site: POTENTIAL. 435
Entrez glycosylation site: POTENTIAL. 454
PRINTS 1: Glycosyl hydrolase family 24..43
PRINTS 2: Glycosyl hydrolase family 60..75
PRINTS 3: Glycosyl hydrolase family 100..121
PRINTS 4: Glycosyl hydrolase family 134..151
PRINTS MELIBIASE1: Melibiase motif I - 2 150..165
PRINTS 5: Glycosyl hydrolase family 166..184
PRINTS MELIBIASE2: Melibiase motif II - 2 213..232
PRINTS 6: Glycosyl hydrolase family 253..272
PRINTS 7: Glycosyl hydrolase family 274..295
PRINTS MELIBIASE3: Melibiase motif III - 2 326..342
PRINTS MELIBIASE4: Melibiase motif IV - 2 351..367
PRINTS MELIBIASE5: Melibiase motif V - 2 374..397
PRINTS MELIBIASE6: Melibiase motif VI - 2 400..422
PRINTS MELIBIASE7: Melibiase motif VII - 2 428..444
PRINTS MELIBIASE8: Melibiase motif VIII - 2 449..469
PRODOM PD018118: MEL1(1) MEL2(1) MEL6(1) 1..22
PRODOM PD002572: AGAL(6) O13295(2) O94221(2) 24..425
PRODOM PD008767: 427..470
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 65..80
__________________
Plus Strand HSPs:
Score = 107 (37.7 bits), Expect = 3.8e-06, Sum P(2) = 3.8e-06
Identities = 29/91 (31%), Positives = 44/91 (48%), Frame = +2
Query: 23 FILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQR-LHD 199
F L C VS S P GW+++++F +SE+ L +A+ +S L D
Sbjct: 4 FYFLTACISLKGVFGVSPSYNGLGLTPQMGWDNWNTFACDVSEQLLLDTADRISDLGLKD 63
Query: 200 HGYEYVVVDYLWYRRKVEGAYHDSLGFDVIDE 295
GY+Y+++D W + DS GF V DE
Sbjct: 64 MGYKYIILDDCW------SSGRDSDGFLVADE 89
Score = 48 (16.9 bits), Expect = 3.8e-06, Sum P(2) = 3.8e-06
Identities = 14/43 (32%), Positives = 22/43 (51%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
D G +V D ++P NG G VA+ +H+ FG++ G
Sbjct: 80 DSDGFLVADEQKFP---NGMG--HVADHLHNNSFLFGMYSSAG 117
>gi|1170910|sp|P41945|MEL2_YEAST ALPHA-GALACTOSIDASE 2 PRECURSOR (MELIBIASE 2)
(ALPHA-D-GALACTOSIDE GALACTOHYDROLASE 2) >gi|1084807|pir||S50310
alpha-galactosidase (EC 3.2.1.22) MEL2 - yeast (Saccharomyces
cerevisiae) (strain VKM Y-1830) >gi|547462|emb|CAA85737.1| (Z37508)
Alpha-Galactosidase [Saccharomyces cerevisiae]
Length = 471
Frame 2 hits (HSPs): _____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | 471
0 150 300 450
__________________
Annotated Domains:
BLOCKS BL00512A: Alpha-galactosidase proteins. 24..44
BLOCKS BL00512B: Alpha-galactosidase proteins. 65..75
BLOCKS BL00512C: Alpha-galactosidase proteins. 79..121
BLOCKS BL00512D: Alpha-galactosidase proteins. 131..153
Entrez glycosylation site: POTENTIAL. 105
Entrez glycosylation site: POTENTIAL. 175
Entrez glycosylation site: POTENTIAL. 270
Entrez glycosylation site: POTENTIAL. 370
Entrez glycosylation site: POTENTIAL. 403
Entrez glycosylation site: POTENTIAL. 413
Entrez glycosylation site: POTENTIAL. 422
Entrez glycosylation site: POTENTIAL. 435
Entrez glycosylation site: POTENTIAL. 454
PRINTS 1: Glycosyl hydrolase family 24..43
PRINTS 2: Glycosyl hydrolase family 60..75
PRINTS 3: Glycosyl hydrolase family 100..121
PRINTS 4: Glycosyl hydrolase family 134..151
PRINTS MELIBIASE1: Melibiase motif I - 2 150..165
PRINTS 5: Glycosyl hydrolase family 166..184
PRINTS MELIBIASE2: Melibiase motif II - 2 213..232
PRINTS 6: Glycosyl hydrolase family 253..272
PRINTS 7: Glycosyl hydrolase family 274..295
PRINTS MELIBIASE3: Melibiase motif III - 2 326..342
PRINTS MELIBIASE4: Melibiase motif IV - 2 351..367
PRINTS MELIBIASE5: Melibiase motif V - 2 374..397
PRINTS MELIBIASE6: Melibiase motif VI - 2 400..422
PRINTS MELIBIASE7: Melibiase motif VII - 2 428..444
PRINTS MELIBIASE8: Melibiase motif VIII - 2 449..469
PRODOM PD018118: MEL1(1) MEL2(1) MEL6(1) 1..22
PRODOM PD002572: AGAL(6) O13295(2) O94221(2) 24..425
PRODOM PD008767: 427..470
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 65..80
__________________
Plus Strand HSPs:
Score = 107 (37.7 bits), Expect = 3.8e-06, Sum P(2) = 3.8e-06
Identities = 29/91 (31%), Positives = 44/91 (48%), Frame = +2
Query: 23 FILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQR-LHD 199
F L C VS S P GW+++++F +SE+ L +A+ +S L D
Sbjct: 4 FYFLTACISLKGVFGVSPSYNGLGLTPQMGWDNWNTFACDVSEQLLLDTADRISDLGLKD 63
Query: 200 HGYEYVVVDYLWYRRKVEGAYHDSLGFDVIDE 295
GY+Y+++D W + DS GF V DE
Sbjct: 64 MGYKYIILDDCW------SSGRDSDGFLVADE 89
Score = 48 (16.9 bits), Expect = 3.8e-06, Sum P(2) = 3.8e-06
Identities = 14/43 (32%), Positives = 22/43 (51%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
D G +V D ++P NG G VA+ +H+ FG++ G
Sbjct: 80 DSDGFLVADEQKFP---NGMG--HVADHLHNNSFLFGMYSSAG 117
>gi|631915|pir||S45453 alpha-galactosidase (EC 3.2.1.22) MEL precursor - yeast
(Zygosaccharomyces cidri) >gi|407012|gb|AAA35280.1| (L24957)
alpha-galactosidase [Zygosaccharomyces cidri]
Length = 469
Frame 2 hits (HSPs): _____________
Annotated Domains: ______________________________________________
__________________________________________________
Database sequence: | | | | | 469
0 150 300 450
__________________
Annotated Domains:
DOMO DM01493: ALPHA-GALACTOSIDASE 10..424
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 64..79
__________________
Plus Strand HSPs:
Score = 103 (36.3 bits), Expect = 2.6e-05, Sum P(2) = 2.6e-05
Identities = 26/71 (36%), Positives = 37/71 (52%), Frame = +2
Query: 23 FILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHD 199
F LF S V + S S P GW++++SF ++ EE L +AE IV L D
Sbjct: 5 FFALFFSSTDVLA--ASPSYNGLGLTPQMGWDNWNSFGCSVKEELLLGTAEKIVKLGLKD 62
Query: 200 HGYEYVVVDYLW 235
GY Y+++D W
Sbjct: 63 LGYNYIILDDCW 74
Score = 53 (18.7 bits), Expect = 2.6e-05, Sum P(2) = 2.6e-05
Identities = 13/40 (32%), Positives = 21/40 (52%), Frame = +2
Query: 299 GRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
G ++ D ++P G VA Q+H+ LKFG++ G
Sbjct: 82 GSLLADDSKFPH-----GMKYVAEQLHNSQLKFGMYSSAG 116
>gi|1246856|emb|CAA64760.1| (X95506) alpha-galactosidase MEL [Saccharomyces
sp.]
Length = 471
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | | 471
0 150 300 450
Plus Strand HSPs:
Score = 106 (37.3 bits), Expect = 2.7e-05, Sum P(2) = 2.7e-05
Identities = 22/73 (30%), Positives = 43/73 (58%), Frame = +2
Query: 23 FILLFLCSESVSSQNVSESEQQQASIPPR-GWNSYDSFCWTISEEEFLQSAEIVSQ-RLH 196
+I LF+ + +V+ S + P+ GW+++++F +SE+ L +A+ +S+ L
Sbjct: 3 YIYLFITAAAVTGALGSSPSYNGLGLTPQMGWDNWNTFACDVSEQLLLNTADRISEIGLK 62
Query: 197 DHGYEYVVVDYLW 235
D GY+YV++D W
Sbjct: 63 DLGYKYVILDDCW 75
Score = 45 (15.8 bits), Expect = 2.7e-05, Sum P(2) = 2.7e-05
Identities = 11/40 (27%), Positives = 19/40 (47%), Frame = +2
Query: 299 GRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
G +V D ++P+ G VA +H+ FG++ G
Sbjct: 83 GTLVADKNKFPN-----GMDHVARHLHNNNFLFGMYSSAG 117
>gi|7481304|pir||T36472 probable secreted alpha-galactosidase - Streptomyces
coelicolor >gi|5824093|emb|CAB54169.1| (AL110470) probable secreted
alpha-galactosidase [Streptomyces coelicolor A3(2)]
Length = 680
Frame 2 hits (HSPs): _________
__________________________________________________
Database sequence: | | | | | | 680
0 150 300 450 600
Plus Strand HSPs:
Score = 118 (41.5 bits), Expect = 3.7e-05, P = 3.7e-05
Identities = 39/122 (31%), Positives = 58/122 (47%), Frame = +2
Query: 71 SESEQQQASIPPRGWNSYDSFCWTISEEEFLQS-AEIVSQRLHDHGYEYVVVDYLWYRRK 247
S +Q A PP G+N+++S T +EF +S + ++ + G + +Y+
Sbjct: 48 SSGHEQLALTPPMGFNNWNS---THCRDEFNESMVKGIADLFVERGLKDAGYEYV----- 99
Query: 248 VEGAYHDSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D D GR+VPDP R+P+ G VA+ VHS GLKFGI+ G T
Sbjct: 100 ---NLDDCWALPERDADGRLVPDPKRFPN-----GIEAVADYVHSKGLKFGIYTSAGTKT 151
Query: 428 FMS 436
S
Sbjct: 152 CSS 154
>gi|2146852|pir||S74221 alpha-galactosidase (EC 3.2.1.22) I precursor - fungus
(Trichoderma reesei) >gi|1580816|emb|CAA93244.1| (Z69253)
alpha-galactosidase [Hypocrea jecorina]
Length = 444
Frame 2 hits (HSPs): __________
Annotated Domains: ___
__________________________________________________
Database sequence: | | | | 444
0 150 300
__________________
Annotated Domains:
PROSITE EF_HAND: EF-hand calcium-binding domain. 52..64
__________________
Plus Strand HSPs:
Score = 115 (40.5 bits), Expect = 4.2e-05, P = 4.2e-05
Identities = 31/80 (38%), Positives = 45/80 (56%), Frame = +2
Query: 98 IPPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLWYRR--KVEG--AY 262
+P GWNS++++ I E +FL +AE IVS L D GY YV +D W + +V+G A
Sbjct: 38 VPSLGWNSWNAYHCDIDESKFLSAAEVIVSSGLLDAGYNYVNIDDCWSMKDGRVDGHIAV 97
Query: 263 HDSLGFDVIDEWGRMVPDPG 322
+ + D ID + V D G
Sbjct: 98 NTTRFPDGIDGLAKKVHDLG 117
>gi|1170912|sp|P41947|MEL6_YEAST ALPHA-GALACTOSIDASE 6 PRECURSOR (MELIBIASE 6)
(ALPHA-D-GALACTOSIDE GALACTOHYDROLASE 6) >gi|1084809|pir||S50312
alpha-galactosidase (EC 3.2.1.22) MEL6 - yeast (Saccharomyces
cerevisiae) (strain CBS4411) >gi|547466|emb|CAA85739.1| (Z37510)
alpha-galactosidase [Saccharomyces cerevisiae]
Length = 471
Frame 2 hits (HSPs): _____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | 471
0 150 300 450
__________________
Annotated Domains:
BLOCKS BL00512A: Alpha-galactosidase proteins. 24..44
BLOCKS BL00512B: Alpha-galactosidase proteins. 65..75
BLOCKS BL00512C: Alpha-galactosidase proteins. 79..121
BLOCKS BL00512D: Alpha-galactosidase proteins. 131..153
Entrez glycosylation site: POTENTIAL. 105
Entrez glycosylation site: POTENTIAL. 175
Entrez glycosylation site: POTENTIAL. 270
Entrez glycosylation site: POTENTIAL. 370
Entrez glycosylation site: POTENTIAL. 403
Entrez glycosylation site: POTENTIAL. 422
Entrez glycosylation site: POTENTIAL. 435
Entrez glycosylation site: POTENTIAL. 454
PRINTS 1: Glycosyl hydrolase family 24..43
PRINTS 2: Glycosyl hydrolase family 60..75
PRINTS 3: Glycosyl hydrolase family 100..121
PRINTS 4: Glycosyl hydrolase family 134..151
PRINTS MELIBIASE1: Melibiase motif I - 2 150..165
PRINTS 5: Glycosyl hydrolase family 166..184
PRINTS MELIBIASE2: Melibiase motif II - 2 213..232
PRINTS 6: Glycosyl hydrolase family 253..272
PRINTS 7: Glycosyl hydrolase family 274..295
PRINTS MELIBIASE3: Melibiase motif III - 2 326..342
PRINTS MELIBIASE4: Melibiase motif IV - 2 351..367
PRINTS MELIBIASE5: Melibiase motif V - 2 374..397
PRINTS MELIBIASE6: Melibiase motif VI - 2 400..422
PRINTS MELIBIASE7: Melibiase motif VII - 2 428..444
PRINTS MELIBIASE8: Melibiase motif VIII - 2 449..469
PRODOM PD018118: MEL1(1) MEL2(1) MEL6(1) 1..22
PRODOM PD002572: AGAL(6) O13295(2) O94221(2) 24..425
PRODOM PD008767: 427..470
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 65..80
__________________
Plus Strand HSPs:
Score = 104 (36.6 bits), Expect = 5.3e-05, Sum P(2) = 5.3e-05
Identities = 29/91 (31%), Positives = 44/91 (48%), Frame = +2
Query: 23 FILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQR-LHD 199
F L C VS S P GW+++++F +SE+ L +A+ +S L D
Sbjct: 4 FYFLTACISLKGVFGVSPSYNGLGLTPQMGWDNWNTFACDVSEQLLLDTADRISDLGLKD 63
Query: 200 HGYEYVVVDYLWYRRKVEGAYHDSLGFDVIDE 295
GY+YV++D W + DS GF V D+
Sbjct: 64 MGYKYVILDDCW------SSGRDSDGFLVADK 89
Score = 48 (16.9 bits), Expect = 5.3e-05, Sum P(2) = 5.3e-05
Identities = 14/43 (32%), Positives = 22/43 (51%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
D G +V D ++P NG G VA+ +H+ FG++ G
Sbjct: 80 DSDGFLVADKHKFP---NGMG--HVADHLHNNSFLFGMYSSAG 117
>gi|10178280|emb|CAC08338.1| (AL392174) alpha-galactosidase-like protein
[Arabidopsis thaliana]
Length = 410
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 410
0 150 300
Plus Strand HSPs:
Score = 91 (32.0 bits), Expect = 5.5e-05, Sum P(2) = 5.5e-05
Identities = 18/46 (39%), Positives = 27/46 (58%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW 235
PP GWNS++ F I E+ ++A+ +V+ L GY YV +D W
Sbjct: 54 PPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNIDDCW 99
Score = 69 (24.3 bits), Expect = 5.5e-05, Sum P(2) = 5.5e-05
Identities = 19/46 (41%), Positives = 23/46 (50%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G +VP +PS G VA+ VHS GLK GI+ G T
Sbjct: 105 DSKGSLVPKKSTFPS-----GIKAVADYVHSKGLKLGIYSDAGYFT 145
>gi|7435462|pir||T10860 alpha-galactosidase (EC 3.2.1.22) - kidney bean
>gi|927577|gb|AAA73964.1| (U12927) alpha-galactosidase [Phaseolus
vulgaris]
Length = 425
Frame 2 hits (HSPs): ___________
__________________________________________________
Database sequence: | | | | 425
0 150 300
Plus Strand HSPs:
Score = 93 (32.7 bits), Expect = 5.9e-05, Sum P(2) = 5.9e-05
Identities = 18/46 (39%), Positives = 29/46 (63%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW 235
PP GWNS++ F I+E+ ++A+ +VS L GY+Y+ +D W
Sbjct: 71 PPMGWNSWNHFSCNINEDLIRETADAMVSTGLAALGYQYINIDDCW 116
Score = 66 (23.2 bits), Expect = 5.9e-05, Sum P(2) = 5.9e-05
Identities = 17/46 (36%), Positives = 22/46 (47%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G +VP +PS G +A+ VH GLK GI+ G T
Sbjct: 122 DSQGNLVPKASTFPS-----GMKALADYVHKKGLKLGIYSDAGTQT 162
>gi|113497|sp|P14749|AGAL_CYATE ALPHA-GALACTOSIDASE PRECURSOR (MELIBIASE)
(ALPHA-D-GALACTOSIDE GALACTOHYDROLASE) >gi|99880|pir||S07472
alpha-galactosidase (EC 3.2.1.22) precursor - guar
>gi|18292|emb|CAA32772.1| (X14619) alpha-galactosidase
preproprotein [Cyamopsis tetragonoloba]
Length = 411
Frame 2 hits (HSPs): _____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | 411
0 150 300
__________________
Annotated Domains:
BLOCKS BL00512A: Alpha-galactosidase proteins. 50..70
BLOCKS BL00512B: Alpha-galactosidase proteins. 91..101
BLOCKS BL00512C: Alpha-galactosidase proteins. 106..148
BLOCKS BL00512D: Alpha-galactosidase proteins. 159..181
DOMO DM01493: ALPHA-GALACTOSIDASE 37..409
Entrez glycosylation site: POTENTIAL. 32
Entrez glycosylation site: POTENTIAL. 145
Entrez glycosylation site: POTENTIAL. 352
Entrez active site: POTENTIAL. 319
PRINTS 1: Glycosyl hydrolase family 50..69
PRINTS 2: Glycosyl hydrolase family 86..101
PRINTS 3: Glycosyl hydrolase family 127..148
PRINTS 4: Glycosyl hydrolase family 162..179
PRINTS 5: Glycosyl hydrolase family 189..207
PRINTS 6: Glycosyl hydrolase family 254..273
PRINTS 7: Glycosyl hydrolase family 275..296
PRODOM PD137378: AGAL_CYATE 1..33
PRODOM PD002572: AGAL(6) O13295(2) O94221(2) 35..409
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 91..107
__________________
Plus Strand HSPs:
Score = 88 (31.0 bits), Expect = 9.7e-05, Sum P(2) = 9.7e-05
Identities = 22/63 (34%), Positives = 33/63 (52%), Frame = +2
Query: 77 SEQQQASIPPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW--YRRK 247
+E PP GWNS++ F I+E ++A+ +VS L GY+Y+ +D W R
Sbjct: 48 AENGLGQTPPMGWNSWNHFGCDINENVVRETADAMVSTGLAALGYQYINLDDCWAELNRD 107
Query: 248 VEG 256
EG
Sbjct: 108 SEG 110
Score = 71 (25.0 bits), Expect = 9.7e-05, Sum P(2) = 9.7e-05
Identities = 18/46 (39%), Positives = 24/46 (52%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G MVP+ +PS G +A+ VHS GLK G++ G T
Sbjct: 107 DSEGNMVPNAAAFPS-----GIKALADYVHSKGLKLGVYSDAGNQT 147
>gi|7435459|pir||T06388 alpha-galactosidase (EC 3.2.1.22) - soybean
>gi|927575|gb|AAA73963.1| (U12926) alpha galactosidase [Glycine
max]
Length = 422
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 422
0 150 300
Plus Strand HSPs:
Score = 93 (32.7 bits), Expect = 0.00015, Sum P(2) = 0.00015
Identities = 18/46 (39%), Positives = 28/46 (60%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW 235
PP GWNS++ F I E+ ++A+ +VS L GY+Y+ +D W
Sbjct: 68 PPMGWNSWNHFACNIKEDLIRETADAMVSTGLAALGYQYINIDDCW 113
Score = 62 (21.8 bits), Expect = 0.00015, Sum P(2) = 0.00015
Identities = 17/46 (36%), Positives = 22/46 (47%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G +VP +PS G +A+ VH GLK GI+ G T
Sbjct: 119 DSKGNLVPKASTFPS-----GMKALADYVHKNGLKLGIYSDAGNQT 159
>gi|6429221|dbj|BAA86883.1| (AB027130) alpha-galactosidase [Torulaspora
delbrueckii]
Length = 474
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | | 474
0 150 300 450
Plus Strand HSPs:
Score = 99 (34.8 bits), Expect = 0.00017, Sum P(2) = 0.00017
Identities = 24/75 (32%), Positives = 38/75 (50%), Frame = +2
Query: 11 SISLFILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAE-IVSQ 187
+ SL + L + V VS S P GWN++++F +SE+ L + + I +
Sbjct: 3 NFSLLTSIVLLASKVVG--VSPSYNGLGLTPQMGWNNWNTFACNVSEDLLLSTVDRIAAL 60
Query: 188 RLHDHGYEYVVVDYLW 235
L D GY YV++D W
Sbjct: 61 GLRDIGYHYVILDDCW 76
Score = 53 (18.7 bits), Expect = 0.00017, Sum P(2) = 0.00017
Identities = 15/51 (29%), Positives = 23/51 (45%), Frame = +2
Query: 266 DSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
D D D G +VPD ++P+ G VA+ +H FG++ G
Sbjct: 73 DDCWSDGRDSDGMLVPDSTKFPN-----GMKHVADYLHGKDFLFGMYSSAG 118
>gi|2492782|sp|Q42656|AGAL_COFAR ALPHA-GALACTOSIDASE PRECURSOR (MELIBIASE)
(ALPHA-D-GALACTOSIDE GALACTOHYDROLASE) >gi|11264279|pir||T50781
alpha-galactosidase (EC 3.2.1.22) [imported] - coffee
>gi|504489|gb|AAA33022.1| (L27992) alpha-galactosidase [Coffea
arabica]
Length = 378
Frame 2 hits (HSPs): _____________
Annotated Domains: _________________________________________________
__________________________________________________
Database sequence: | | | | 378
0 150 300
__________________
Annotated Domains:
BLOCKS BL00512A: Alpha-galactosidase proteins. 18..38
BLOCKS BL00512B: Alpha-galactosidase proteins. 59..69
BLOCKS BL00512C: Alpha-galactosidase proteins. 74..116
BLOCKS BL00512D: Alpha-galactosidase proteins. 127..149
Entrez active site: POTENTIAL. 287
PRINTS 1: Glycosyl hydrolase family 18..37
PRINTS 2: Glycosyl hydrolase family 54..69
PRINTS 3: Glycosyl hydrolase family 95..116
PRINTS 4: Glycosyl hydrolase family 130..147
PRINTS 5: Glycosyl hydrolase family 157..175
PRINTS 6: Glycosyl hydrolase family 222..241
PRINTS 7: Glycosyl hydrolase family 243..264
PRODOM PD002572: AGAL(6) O13295(2) O94221(2) 12..376
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 59..75
__________________
Plus Strand HSPs:
Score = 86 (30.3 bits), Expect = 0.00024, Sum P(2) = 0.00024
Identities = 17/46 (36%), Positives = 29/46 (63%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW 235
PP GWNS++ F + E+ ++A+ +VS+ L GY+Y+ +D W
Sbjct: 24 PPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCW 69
Score = 69 (24.3 bits), Expect = 0.00024, Sum P(2) = 0.00024
Identities = 18/46 (39%), Positives = 23/46 (50%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G +VP +PS G +A+ VHS GLK GI+ G T
Sbjct: 75 DSQGNLVPKGSTFPS-----GIKALADYVHSKGLKLGIYSDAGTQT 115
>gi|10140702|gb|AAG13536.1|AC023240_9 (AC023240) putative alpha-galactosidase
[Oryza sativa]
Length = 413
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 413
0 150 300
Plus Strand HSPs:
Score = 108 (38.0 bits), Expect = 0.00043, P = 0.00043
Identities = 37/112 (33%), Positives = 54/112 (48%), Frame = +2
Query: 92 ASIPPRGWNSYDSF-CWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLWYRRKVEGAYH 265
A+ PP GWNS++ F C E+ ++A+ +VS L GY+YV +D W +
Sbjct: 44 ATAPPMGWNSWNHFACDGNGEDVIRETADALVSTGLAAAGYKYVNIDDCWAEPQ-----R 98
Query: 266 DSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
DS G +V + +P G +A+ VHS GLK GI+ G T
Sbjct: 99 DSKG--------NLVANKKTFPH-----GIKALADYVHSKGLKLGIYSDAGFKT 139
>gi|9971577|dbj|BAB12570.1| (AB039671) alpha-galactosidase [Oryza sativa subsp.
japonica]
Length = 417
Frame 2 hits (HSPs): ____________ ______
__________________________________________________
Database sequence: | | | | 417
0 150 300
Plus Strand HSPs:
Score = 88 (31.0 bits), Expect = 0.00066, Sum P(2) = 0.00066
Identities = 20/57 (35%), Positives = 33/57 (57%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW--YRRKVEGAY 262
P GWNS++ F I+E+ ++A+ +V+ L GY+YV +D W Y R +G +
Sbjct: 64 PQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAEYSRDSQGNF 120
Score = 63 (22.2 bits), Expect = 0.00066, Sum P(2) = 0.00066
Identities = 17/46 (36%), Positives = 23/46 (50%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G VP+ +PS G +A+ VH+ GLK GI+ G T
Sbjct: 115 DSQGNFVPNRQTFPS-----GIKALADYVHAKGLKLGIYSDAGSQT 155
Score = 36 (12.7 bits), Expect = 0.36, Sum P(2) = 0.30
Identities = 11/38 (28%), Positives = 14/38 (36%), Frame = +2
Query: 281 DVIDEWGRMVP---DPGRW-----PSSENGKGFTEVAN 370
D+ D WG M + +W P N EV N
Sbjct: 240 DIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGN 277
>gi|82899|pir||JQ1021 alpha-galactosidase (EC 3.2.1.22) - yeast (Saccharomyces
cerevisiae) (strain carlsbergensis) >gi|171924|gb|AAA34769.1|
(M58484) alpha-galactosidase [Saccharomyces pastorianus]
Length = 471
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | | 471
0 150 300 450
Plus Strand HSPs:
Score = 99 (34.8 bits), Expect = 0.00084, Sum P(2) = 0.00084
Identities = 24/75 (32%), Positives = 42/75 (56%), Frame = +2
Query: 20 LFIL-LFLCSESVSSQNVSESEQQQASIPPR-GWNSYDSFCWTISEEEFLQSAEIVSQ-R 190
+F+L LF +VS S + P+ GW+++++F +SE+ L +A+ +S+
Sbjct: 1 MFLLYLFTSFAAVSGVLGSSPSYNGLGLTPQMGWDNWNTFACDVSEQLLLDTADRISEIG 60
Query: 191 LHDHGYEYVVVDYLW 235
L D GY YV++D W
Sbjct: 61 LKDLGYTYVILDDCW 75
Score = 46 (16.2 bits), Expect = 0.00084, Sum P(2) = 0.00084
Identities = 11/40 (27%), Positives = 21/40 (52%), Frame = +2
Query: 299 GRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
G +V D ++P+ G + VA+ +H+ FG++ G
Sbjct: 83 GTLVADKEKFPN-----GMSHVADHLHNNNFLFGMYSSAG 117
>gi|631001|pir||S45522 alpha-N-acetylgalactosaminidase - chicken
>gi|435037|gb|AAA16614.1| (L18754) alpha-N-acetylgalactosaminidase
[Gallus gallus]
Length = 405
Frame 2 hits (HSPs): _____________
Annotated Domains: ________________________________________________
__________________________________________________
Database sequence: | | | | 405
0 150 300
__________________
Annotated Domains:
DOMO DM01493: ALPHA-GALACTOSIDASE 1..386
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 54..70
__________________
Plus Strand HSPs:
Score = 102 (35.9 bits), Expect = 0.0093, P = 0.0092
Identities = 36/109 (33%), Positives = 55/109 (50%), Frame = +2
Query: 80 EQQQASIPPRGWNSYDSF-C---------WTISEEEFLQSAE-IVSQRLHDHGYEYVVVD 226
E A PP GW +++ F C ISE F++ A+ I + GY+Y+ +D
Sbjct: 2 ENGLARTPPMGWLAWERFRCNVNCREDPRQCISEMLFMEMADRIAEDGWRELGYKYINID 61
Query: 227 YLWYRRKVEGAYHDSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIH 406
W ++ D GR+VPDP R+P +G +A+ VH+ GLK I+
Sbjct: 62 DCWAAKQR-------------DAEGRLVPDPERFP-----RGIKALADYVHARGLKLDIY 103
>gi|631000|pir||S43413 alpha-N-acetylgalactosamindase - chicken
Length = 405
Frame 2 hits (HSPs): _____________
Annotated Domains: ________________________________________________
__________________________________________________
Database sequence: | | | | 405
0 150 300
__________________
Annotated Domains:
DOMO DM01493: ALPHA-GALACTOSIDASE 1..386
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 54..70
__________________
Plus Strand HSPs:
Score = 102 (35.9 bits), Expect = 0.0093, P = 0.0092
Identities = 36/109 (33%), Positives = 55/109 (50%), Frame = +2
Query: 80 EQQQASIPPRGWNSYDSF-C---------WTISEEEFLQSAE-IVSQRLHDHGYEYVVVD 226
E A PP GW +++ F C ISE F++ A+ I + GY+Y+ +D
Sbjct: 2 ENGLARTPPMGWLAWERFRCNVNCREDPRQCISEMLFMEMADRIAEDGWRELGYKYINID 61
Query: 227 YLWYRRKVEGAYHDSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIH 406
W ++ D GR+VPDP R+P +G +A+ VH+ GLK I+
Sbjct: 62 DCWAAKQR-------------DAEGRLVPDPERFP-----RGIKALADYVHARGLKLDIY 103
>gi|105289|pir||A33265 alpha-N-acetylgalactosaminidase (EC 3.2.1.49) precursor,
splice form 1 - human >gi|189053|gb|AAA59902.1| (M29276)
alpha-N-acetylgalactosaminidase [Homo sapiens]
Length = 358
Frame 2 hits (HSPs): ________________
Annotated Domains: ____ ___
__________________________________________________
Database sequence: | | | | 358
0 150 300
__________________
Annotated Domains:
PROSITE ALPHA_GALACTOSIDASE: Alpha-galactosidase 71..86
PROSITE LEUCINE_ZIPPER: Leucine zipper pattern. 2..23
__________________
Plus Strand HSPs:
Score = 100 (35.2 bits), Expect = 0.016, P = 0.016
Identities = 34/115 (29%), Positives = 51/115 (44%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDSLGF 280
PP GW +++ F I+ +E +S++L + + D W D
Sbjct: 26 PPMGWLAWERFRCNINCDE--DPKNCISEQLFMEMADRMAQDG-WRDMGYTYLNIDDCWI 82
Query: 281 DVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGISTFMS*EG 445
D GR++PDP R+P G +A+ VHS+GLK GI+ G T M G
Sbjct: 83 GGRDASGRLMPDPKRFPH-----GIPFLADYVHSLGLKLGIYADMGNFTCMGYPG 132
>gi|4557781 ref|NP_000253.1| alpha-N-acetylgalactosaminidase precursor;
Acetylgalactosaminidase, alpha-N- (alpha-galactosidase B) [Homo
sapiens] >gi|11418199 ref|XP_010024.1|
alpha-N-acetylgalactosaminidase precursor [Homo sapiens]
>gi|127801|sp|P17050|NAGA_HUMAN ALPHA-N-ACETYLGALACTOSAMINIDASE
PRECURSOR (ALPHA-GALACTOSIDASE B) >gi|105291|pir||A35485
alpha-N-acetylgalactosaminidase (EC 3.2.1.49) precursor, splice
form 2 - human >gi|178248|gb|AAA51677.1| (M62783)
alpha-N-acetylgalactosaminidase [Homo sapiens]
>gi|189055|gb|AAA36351.1| (M38083) alpha-N-acetylgalactosaminidase
[Homo sapiens] >gi|1513067|gb|AAB06718.1| (M59199)
alpha-N-acetylgalactosaminidase [Homo sapiens]
>gi|5578716|emb|CAB41237.1| (Z99716) bK250D10.5
(alpha-N-acetylgalactosaminidase) [Homo sapiens]
>gi|12652693|gb|AAH00095.1|AAH00095 (BC000095)
N-acetylgalactosaminidase, alpha- [Homo sapiens]
Length = 411
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 411
0 150 300
Plus Strand HSPs:
Score = 100 (35.2 bits), Expect = 0.020, P = 0.020
Identities = 34/115 (29%), Positives = 51/115 (44%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDSLGF 280
PP GW +++ F I+ +E +S++L + + D W D
Sbjct: 26 PPMGWLAWERFRCNINCDE--DPKNCISEQLFMEMADRMAQDG-WRDMGYTYLNIDDCWI 82
Query: 281 DVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGISTFMS*EG 445
D GR++PDP R+P G +A+ VHS+GLK GI+ G T M G
Sbjct: 83 GGRDASGRLMPDPKRFPH-----GIPFLADYVHSLGLKLGIYADMGNFTCMGYPG 132
>gi|10944324|gb|AAG24510.1|AF246262_1 (AF246262) alpha-galactosidase
[Phanerochaete chrysosporium]
Length = 438
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 438
0 150 300
Plus Strand HSPs:
Score = 100 (35.2 bits), Expect = 0.022, P = 0.022
Identities = 31/112 (27%), Positives = 59/112 (52%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDS 271
A P GWN+++ F ISE+ L +A+ + L + + D+ + ++ +H +
Sbjct: 26 AITPQMGWNTWNHFGCDISEDTILSAAQAI---LASN-----LTDFGYEYVIMDDCWH-A 76
Query: 272 LGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D ++ G V DP ++P+ G ++++++H+MGLKFGI+ G T
Sbjct: 77 PSRD--NQTGAPVADPSKFPN-----GIKDLSDKIHAMGLKFGIYSDAGTYT 121
>gi|3396057|gb|AAC28851.1| (AF079458) alpha-N-acetylgalactosaminidase [Mus
musculus]
Length = 415
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 415
0 150 300
Plus Strand HSPs:
Score = 98 (34.5 bits), Expect = 0.042, P = 0.041
Identities = 37/115 (32%), Positives = 53/115 (46%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYH--DSL 274
PP GW +++ F I E +S+RL + + D W R + Y D
Sbjct: 26 PPMGWLAWERFRCNIDCVE--DPKNCISERLFMEMADRLAQDG-W--RDLGYVYLNIDDC 80
Query: 275 GFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGISTFMS*EG 445
D GR++PDP R+P +G F +A+ HS+GLK GI+ G T M G
Sbjct: 81 WIGGRDASGRLIPDPKRFP---HGIAF--LADYAHSLGLKLGIYEDMGKMTCMGYPG 132
>gi|1246850|emb|CAA64759.1| (X95505) alpha-galactosidase MEL [Saccharomyces
paradoxus]
Length = 471
Frame 2 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | 471
0 150 300 450
Plus Strand HSPs:
Score = 98 (34.5 bits), Expect = 0.050, P = 0.049
Identities = 23/65 (35%), Positives = 37/65 (56%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAEIVSQ-RLHDHGYEYVVVDYLWYRRKVEGAYHDSLG 277
P GW+++++F +SE+ L +A+ +S L D GY+YV++D W + D G
Sbjct: 30 PQMGWDNWNTFACNVSEDLLLNTADRISDIGLKDLGYKYVILDDCW------SSGRDEDG 83
Query: 278 FDVIDE 295
F V DE
Sbjct: 84 FLVADE 89
>gi|10944326|gb|AAG24511.1|AF246263_1 (AF246263) alpha-galactosidase
[Phanerochaete chrysosporium]
Length = 438
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 438
0 150 300
Plus Strand HSPs:
Score = 97 (34.1 bits), Expect = 0.062, P = 0.060
Identities = 32/112 (28%), Positives = 60/112 (53%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKV-EGAYHD 268
A P GWN+++ F ISE+ L +A+ + + ++ + D L Y + + +H
Sbjct: 26 AITPQMGWNTWNHFGCDISEDTILSAAQAI---IANN-----LTD-LGYEYVIMDDCWH- 75
Query: 269 SLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
+ D ++ G V DP ++P+ G ++++++H+MGLKFGI+ G T
Sbjct: 76 APSRD--NQTGAPVADPAKFPN-----GIKDLSDKIHAMGLKFGIYSDAGTYT 121
>gi|10312171|gb|AAG16693.1| (AF289080) alpha-galactosidase [Lycopersicon
esculentum]
Length = 380
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 380
0 150 300
Plus Strand HSPs:
Score = 71 (25.0 bits), Expect = 0.25, Sum P(2) = 0.22
Identities = 16/46 (34%), Positives = 24/46 (52%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW 235
P GW+S++ F I E +A+ +V L GYEY+ +D W
Sbjct: 54 PQMGWSSWNHFGCNIDENIIKGTADAMVHTGLASLGYEYINIDDCW 99
Score = 59 (20.8 bits), Expect = 0.25, Sum P(2) = 0.22
Identities = 16/46 (34%), Positives = 21/46 (45%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G MV +PS G +A+ VH GLK G++ G T
Sbjct: 105 DSQGNMVAKGSTFPS-----GIKALADYVHGKGLKLGVYSDAGTQT 145
>gi|6164944|gb|AAF04591.1|AF191823_1 (AF191823) alpha-galactosidase
[Lycopersicon esculentum]
Length = 409
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 409
0 150 300
Plus Strand HSPs:
Score = 71 (25.0 bits), Expect = 0.30, Sum P(2) = 0.26
Identities = 16/46 (34%), Positives = 24/46 (52%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLW 235
P GW+S++ F I E +A+ +V L GYEY+ +D W
Sbjct: 54 PQMGWSSWNHFGCNIDENIIKGTADAMVHTGLASLGYEYINIDDCW 99
Score = 59 (20.8 bits), Expect = 0.30, Sum P(2) = 0.26
Identities = 16/46 (34%), Positives = 21/46 (45%), Frame = +2
Query: 290 DEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D G MV +PS G +A+ VH GLK G++ G T
Sbjct: 105 DSQGNMVAKGSTFPS-----GIKALADYVHGKGLKLGVYSDAGTQT 145
>gi|7305089 ref|NP_038491.1| galactosidase, alpha; alpha-galactosidase [Mus
musculus] >gi|1703210|sp|P51569|AGAL_MOUSE ALPHA-GALACTOSIDASE A
PRECURSOR (MELIBIASE) (ALPHA-D-GALACTOSIDE GALACTOHYDROLASE)
(ALPHA-D-GALACTOSIDASE A) >gi|2118040|pir||JC4522
alpha-galactosidase (EC 3.2.1.22) A precursor - mouse
>gi|945409|gb|AAA74453.1| (L46651) alpha-D-galactosidase A [Mus
musculus] >gi|1141788|gb|AAA96749.1| (U34071) alpha-galactosidase A
[Mus musculus] >gi|1335898|gb|AAC52583.1| (U50715)
alpha-galactosidase A [Mus musculus] >gi|1335900|gb|AAC52584.1|
(U50716) alpha-galactosidase A [Mus musculus]
>gi|1666701|gb|AAB47244.1| (U58105) alpha-D-galactosidase A [Mus
musculus]
Length = 419
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 419
0 150 300
Plus Strand HSPs:
Score = 90 (31.7 bits), Expect = 0.52, P = 0.41
Identities = 34/112 (30%), Positives = 48/112 (42%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDS 271
A P GW ++ F + +E + +S++L E +V D W + D
Sbjct: 37 ARTPTMGWLHWERFMCNLDCQE--EPDACISEQLFMQMAELMVSDG-WRDAGYDYLCIDD 93
Query: 272 LGF-DVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D GR+ DP R+PS G +AN VHS GLK GI+ G T
Sbjct: 94 CWMAPERDSKGRLQADPQRFPS-----GIKHLANYVHSKGLKLGIYADVGNKT 141
>gi|6679006 ref|NP_032695.1| N-acetyl galactosaminidase, alpha [Mus musculus]
>gi|3327016|emb|CAA11703.1| (AJ223966)
alpha-N-acetylgalactosaminidase [Mus musculus]
Length = 415
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 415
0 150 300
Plus Strand HSPs:
Score = 89 (31.3 bits), Expect = 0.69, P = 0.50
Identities = 36/115 (31%), Positives = 51/115 (44%), Frame = +2
Query: 101 PPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYH--DSL 274
PP GW +++ F I E +S L + + D W R + Y D
Sbjct: 26 PPMGWLAWERFRCNIDCVE--DPKNCISDGLFMEMADRLAQDG-W--RDLGYVYLNIDDC 80
Query: 275 GFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGISTFMS*EG 445
D GR++PDP R+P +G F +A+ HS+GLK GI+ G T M G
Sbjct: 81 WIGGRDASGRLIPDPKRFP---HGIAF--LADYAHSLGLKLGIYEDMGKMTCMGYPG 132
>gi|178246|gb|AAA51676.1| (M13571) alpha-galactosidase A precursor (EC
3.2.1.22) [Homo sapiens] >gi|3808178|dbj|BAA34059.1| (D00039)
alpha-galactosidase A [Homo sapiens]
Length = 403
Frame 2 hits (HSPs): ______________
__________________________________________________
Database sequence: | | | | 403
0 150 300
Plus Strand HSPs:
Score = 88 (31.0 bits), Expect = 0.87, P = 0.58
Identities = 34/112 (30%), Positives = 48/112 (42%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDS 271
A P GW ++ F + +E S +S++L E +V + W E D
Sbjct: 11 ARTPTMGWLHWERFMCNLDCQEEPDSC--ISEKLFMEMAELMVSEG-WKDAGYEYLCIDD 67
Query: 272 LGF-DVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D GR+ DP R+P G ++AN VHS GLK GI+ G T
Sbjct: 68 CWMAPQRDSEGRLQADPQRFPH-----GIRQLANYVHSKGLKLGIYADVGNKT 115
>gi|4504009 ref|NP_000160.1| galactosidase, alpha [Homo sapiens]
>gi|113499|sp|P06280|AGAL_HUMAN ALPHA-GALACTOSIDASE A PRECURSOR
(MELIBIASE) (ALPHA-D-GALACTOSIDE GALACTOHYDROLASE)
(ALPHA-D-GALACTOSIDASE A) >gi|2144478|pir||GBHUA
alpha-galactosidase (EC 3.2.1.22) A precursor - human
>gi|31756|emb|CAA32617.1| (X14448) alpha-D-galactosidase A [Homo
sapiens] >gi|757912|emb|CAA29232.1| (X05790) alpha-galactosidase
[Homo sapiens] >gi|1684916|gb|AAB64203.1| (U78027)
alpha-D-galactosidase A [Homo sapiens] >gi|5911813|emb|CAB55878.1|
(AL035422) dJ164F3.2 (galactosidase, alpha) [Homo sapiens]
>gi|226967|prf||1612342A alpha galactosidase [Homo sapiens]
Length = 429
Frame 2 hits (HSPs): _____________
__________________________________________________
Database sequence: | | | | 429
0 150 300
Plus Strand HSPs:
Score = 88 (31.0 bits), Expect = 0.95, P = 0.61
Identities = 34/112 (30%), Positives = 48/112 (42%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDS 271
A P GW ++ F + +E S +S++L E +V + W E D
Sbjct: 37 ARTPTMGWLHWERFMCNLDCQEEPDSC--ISEKLFMEMAELMVSEG-WKDAGYEYLCIDD 93
Query: 272 LGF-DVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D GR+ DP R+P G ++AN VHS GLK GI+ G T
Sbjct: 94 CWMAPQRDSEGRLQADPQRFPH-----GIRQLANYVHSKGLKLGIYADVGNKT 141
>gi|11419728 ref|XP_010108.1| galactosidase, alpha [Homo sapiens]
Length = 449
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 449
0 150 300
Plus Strand HSPs:
Score = 88 (31.0 bits), Expect = 1.0, P = 0.64
Identities = 34/112 (30%), Positives = 48/112 (42%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAEIVSQRLHDHGYEYVVVDYLWYRRKVEGAYHDS 271
A P GW ++ F + +E S +S++L E +V + W E D
Sbjct: 57 ARTPTMGWLHWERFMCNLDCQEEPDSC--ISEKLFMEMAELMVSEG-WKDAGYEYLCIDD 113
Query: 272 LGF-DVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRGIST 427
D GR+ DP R+P G ++AN VHS GLK GI+ G T
Sbjct: 114 CWMAPQRDSEGRLQADPQRFPH-----GIRQLANYVHSKGLKLGIYADVGNKT 161
>gi|207854|gb|AAA72859.1| (M35837) alpha-amylase/alpha-galactosidase fusion
protein [synthetic construct]
Length = 65
Frame 2 hits (HSPs): ___________________________________________
__________________________________________________
Database sequence: | | | | | 65
0 20 40 60
Plus Strand HSPs:
Score = 67 (23.6 bits), Expect = 1.0, P = 0.65
Identities = 16/55 (29%), Positives = 26/55 (47%), Frame = +2
Query: 11 SISLFILLFLCSESVSSQNVSESEQQQASIPPRGWNSYDSFCWTISEEEFLQSAE 175
S L ++ L S+ S +E PP GWNS++ F I+E ++A+
Sbjct: 10 SFRLVLMCTLLFVSLPITKTSAAENGLGQTPPMGWNSWNHFGCDINENVVRETAD 64
>gi|11275340|dbj|BAB18272.1| (AB038520) alpha-galactosidase [Saccharomyces
kluyveri]
Length = 416
Frame 2 hits (HSPs): __________
__________________________________________________
Database sequence: | | | | 416
0 150 300
Plus Strand HSPs:
Score = 68 (23.9 bits), Expect = 1.2, Sum P(2) = 0.69
Identities = 17/45 (37%), Positives = 24/45 (53%), Frame = +2
Query: 131 FCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLWYRRKVEGAY 262
F +SE+ L ++E IV L D GY+YVV+D W + Y
Sbjct: 1 FACDVSEDLLLGTSEKIVKWGLKDLGYKYVVLDDCWSSGRNSSGY 45
Score = 57 (20.1 bits), Expect = 1.2, Sum P(2) = 0.69
Identities = 13/40 (32%), Positives = 21/40 (52%), Frame = +2
Query: 299 GRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIHVMRG 418
G +VPD ++P +G V + +H GL FG++ G
Sbjct: 44 GYLVPDSKKFP-----RGMKHVGDSLHDEGLLFGMYSSAG 78
>gi|11264291|pir||T47748 alpha-galactosidase-like protein - Arabidopsis
thaliana >gi|7572929|emb|CAB87430.1| (AL163763)
alpha-galactosidase-like protein [Arabidopsis thaliana]
Length = 434
Frame 2 hits (HSPs): _________
__________________________________________________
Database sequence: | | | | 434
0 150 300
Plus Strand HSPs:
Score = 87 (30.6 bits), Expect = 1.3, P = 0.74
Identities = 27/81 (33%), Positives = 41/81 (50%), Frame = +2
Query: 92 ASIPPRGWNSYDSFCWTISEEEFLQSAE-IVSQRLHDHGYEYVVVDYLWYRRKVEGAYHD 268
A P GWNS++ F I+E ++A+ +VS L D GY +V +D W
Sbjct: 67 ARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCW----------S 116
Query: 269 SLGFDVIDEWGRMVPDPGRWPS 334
+L + D G++VP P +PS
Sbjct: 117 NL---LRDSEGQLVPHPETFPS 135
>gi|7504116|pir||T33837 hypothetical protein F54A5.3b - Caenorhabditis elegans
>gi|3886078|gb|AAC78223.1| (AF106584) F54A5.3b gene product
[Caenorhabditis elegans]
Length = 81
Frame -3 hits (HSPs): ______________________
__________________________________________________
Database sequence: | | | | | | 81
0 20 40 60 80
Minus Strand HSPs:
Score = 65 (22.9 bits), Expect = 1.8, P = 0.83
Identities = 13/36 (36%), Positives = 20/36 (55%), Frame = -3
Query: 310 NHPPPFINHIKSERIMISALYFPSIPEVIHYNILVS 203
N P F ++S + +SA Y S+P V N++VS
Sbjct: 3 NVEPSFAEELRSSGVSLSATYLGSVPVVESINVMVS 38
>gi|7504117|pir||T33838 hypothetical protein F54A5.3c - Caenorhabditis elegans
>gi|3886079|gb|AAC78224.1| (AF106584) F54A5.3c gene product
[Caenorhabditis elegans]
Length = 52
Frame -3 hits (HSPs): ___________________________________
__________________________________________________
Database sequence: | | | | 52
0 20 40
Minus Strand HSPs:
Score = 65 (22.9 bits), Expect = 1.8, P = 0.83
Identities = 13/36 (36%), Positives = 20/36 (55%), Frame = -3
Query: 310 NHPPPFINHIKSERIMISALYFPSIPEVIHYNILVS 203
N P F ++S + +SA Y S+P V N++VS
Sbjct: 3 NVEPSFAEELRSSGVSLSATYLGSVPVVESINVMVS 38
>gi|5733875|gb|AAD49763.1|AC007932_11 (AC007932) F11A17.11 [Arabidopsis
thaliana]
Length = 75
Frame -2 hits (HSPs): _______________________
__________________________________________________
Database sequence: | | | | | 75
0 20 40 60
Minus Strand HSPs:
Score = 63 (22.2 bits), Expect = 3.0, P = 0.95
Identities = 13/33 (39%), Positives = 19/33 (57%), Frame = -2
Query: 353 ILSHFQKKATFLGQEPSSPIHQSHQIRENHDKR 255
I + F+K ATF Q + H + + RE H+KR
Sbjct: 8 IATPFKKAATFFNQPQQTNRHANAKAREEHEKR 40
>gi|7302937|gb|AAF58008.1| (AE003807) CG7997 gene product [Drosophila
melanogaster]
Length = 384
Frame 2 hits (HSPs): ______________
__________________________________________________
Database sequence: | | | | 384
0 150 300
Plus Strand HSPs:
Score = 83 (29.2 bits), Expect = 3.5, P = 0.97
Identities = 33/103 (32%), Positives = 53/103 (51%), Frame = +2
Query: 110 GWNSYDSF-CWT---------ISEEEFLQSAEI-VSQRLHDHGYEYVVVDYLWYRRKVEG 256
GW S++ F C T ISE+ F + A++ VS+ D GYEYV++D W + +
Sbjct: 2 GWMSWERFRCITDCKLYPDECISEKLFQRHADLLVSEGYADAGYEYVIIDDCWLEKNRD- 60
Query: 257 AYHDSLGFDVIDEWGRMVPDPGRWPSSENGKGFTEVANQVHSMGL--KFGIHVMRG 418
++ ++VPD R+P+ N + NQ GL +G + G
Sbjct: 61 -----------NDTQKLVPDRKRFPNGLNALS-DHIHNQGLKFGLYQDYGTNTCAG 104
>gi|7297618|gb|AAF52871.1| (AE003627) CG5731 gene product [Drosophila
melanogaster]
Length = 386
Frame 2 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 386
0 150 300
Plus Strand HSPs:
Score = 65 (22.9 bits), Expect = 5.7, Sum P(2) = 1.0
Identities = 14/36 (38%), Positives = 23/36 (63%), Frame = +2
Query: 299 GRMVPDPGRWPSSENGKGFTEVANQVHSMGLKFGIH 406
G++V D R+P+ G +++ +HS GLKFGI+
Sbjct: 63 GKLVADRKRFPN-----GIKALSDYIHSRGLKFGIY 93
Score = 53 (18.7 bits), Expect = 5.7, Sum P(2) = 1.0
Identities = 18/56 (32%), Positives = 26/56 (46%), Frame = +2
Query: 110 GWNSYDSF-CWT---------ISEEEFLQSAEIV-SQRLHDHGYEYVVVDYLWYRR 244
GW S++ F C T ISE+ F +IV + GYEY+ +D W +
Sbjct: 2 GWLSWERFRCNTDCVNDPDNCISEQLFQTMTDIVVADGYASVGYEYINIDDCWLEK 57
WARNING: HSPs involving 1 database sequences were not reported due to the
limiting value of parameter B = 50.
Parameters:
filter=none
matrix=BLOSUM62
V=50
B=50
E=10
gi
H=1
sort_by_pvalue
echofilter
ctxfactor=5.99
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
Std. 0 BLOSUM62 0.318 0.135 0.401
+3 0 BLOSUM62 0.318 0.135 0.401 0.354 0.159 0.546
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+2 0 BLOSUM62 0.318 0.135 0.401 0.325 0.139 0.446
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+1 0 BLOSUM62 0.318 0.135 0.401 0.381 0.171 0.694
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-1 0 BLOSUM62 0.318 0.135 0.401 0.350 0.152 0.524
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-2 0 BLOSUM62 0.318 0.135 0.401 0.345 0.150 0.478
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-3 0 BLOSUM62 0.318 0.135 0.401 0.342 0.152 0.518
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+3 0 150 150 10. 74 3 12 22 0.092 34
30 0.12 36
+2 0 150 149 10. 74 3 12 22 0.092 34
30 0.12 36
+1 0 151 150 10. 74 3 12 22 0.092 34
30 0.12 36
-1 0 151 150 10. 74 3 12 22 0.092 34
30 0.12 36
-2 0 150 150 10. 74 3 12 22 0.092 34
30 0.12 36
-3 0 150 150 10. 74 3 12 22 0.092 34
30 0.12 36
Statistics:
Database: /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
Title: nr
Release date: unknown
Posted date: 4:06 PM CST Feb 28, 2001
Format: BLAST
# of letters in database: 197,782,623
# of sequences in database: 625,274
# of database sequences satisfying E: 51
No. of states in DFA: 593 (58 KB)
Total size of DFA: 182 KB (192 KB)
Time to generate neighborhood: 0.01u 0.01s 0.02t Elapsed: 00:00:00
No. of threads or processors used: 6
Search cpu time: 152.38u 0.98s 153.36t Elapsed: 00:00:29
Total cpu time: 152.43u 1.05s 153.48t Elapsed: 00:00:29
Start: Wed Feb 6 11:43:55 2002 End: Wed Feb 6 11:44:24 2002
WARNINGS ISSUED: 2
Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000