Collins LA, Franzblau SG; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1997 May 4-8; 97: 555 (abstract no. U70).
GWL Hansen's Disease Center, Baton Rouge, LA.
In response to the need for rapid, inexpensive, high throughput assays for anti-mycobacterial drug screening, we evaluated a microbroth dilution assay performed in 96-well microplates which uses Alamar blue reagent for determination of growth. Minimum inhibitory concentrations (MICs) of 30 antimicrobial agents against Mycobacterium tuberculosis H37Rv, M. tuberculosis H37Ra and M. avium were determined in the microplate Alamar Blue assay (MABA) using both visual and fluorometric readings and compared to MICs determined in the BACTEC 460. For all three mycobacterial strains, there was 1 dilution difference between MABA and BACTEC median MICs of four replicate experiments for 25-27 of the 30 antimicrobics. Overall, MICs determined either visually or fluorometrically in MABA were highly correlated with those determined in the BACTEC 460 system and visual and fluorometric MABA MICs were highly correlated with each other. Differences in the MICs between assays were only notable with thiacetazone and clarithromycin. MICs of rifampin, rifabutin and clarithromycin were consistently lower for H37Ra compared to H37Rv in all assays but were similar for most other drugs. M. tuberculosis H37Ra appears to be a suitable surrogate for the more virulent H37Rv strain in primary screening of compounds for anti-tuberculosis activity. MABA is sensitive, rapid, inexpensive, non-radiometric and offers the potential for screening, with or without analytical instrumentation, large numbers of antimicrobial compounds against slow-growing mycobacteria.
Publication Types:
Keywords:
- Anti-Infective Agents
- Biological Assay
- Clarithromycin
- Drug Evaluation, Preclinical
- Indicators and Reagents
- Microbial Sensitivity Tests
- Mycobacterium
- Mycobacterium avium
- Mycobacterium tuberculosis
- Oxazines
- Rifampin
- Tuberculosis
- Xanthenes
- resazurin
Other ID:
UI: 102235479
From Meeting Abstracts