NCI_CGAP_Lu25

Library Description

Organism:	Homo sapiens
Developmental stage:	adult
Organ:	lung
Tissue:	bronchioalveolar carcinoma
Vector:	pAMP1
Vector type:	plasmid (ampicillin resistant)
Lab host:	DH10B

mRNA made from lung carcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified.

Source: Robert Strausberg, Ph.D.

Gene Content Analysis

800 ESTs from this library were grouped into 170 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
82	102500	Hs.111903	Fc fragment of IgG, receptor, transporter, alpha.
55	68750	Hs.485158	SAM pointed domain containing ets transcription factor.
38	47500	Hs.510635	Immunoglobulin heavy constant gamma 1 (G1m marker).
30	37500	Hs.494614	BAT2 domain containing 1.
29	36250	Hs.449585	Immunoglobulin lambda locus.
28	35000	Hs.437277	Sequestosome 1.
21	26250	Hs.446522	Ribosomal protein L14.
17	21250	Hs.355264	Cytochrome b-561.
15	18750	Hs.567699	Transcribed locus, strongly similar to NP_003964.3 ribosomal protein L14 [Homo sapiens].
15	18750	Hs.501578	Mitochondrial GTPase 1 homolog (S. cerevisiae).
13	16250	Hs.415342	Transcription factor 25 (basic helix-loop-helix).
13	16250	Hs.328801	Transcribed locus.
12	15000	Hs.55016	EPS8-like 2.
11	13750	Hs.677030	Hypothetical LOC728875.
10	12500	Hs.517331	Chromosome 21 open reading frame 2.
10	12500	Hs.314359	Eukaryotic translation initiation factor 3, subunit K.
10	12500	Hs.310781	Chromosome 5 open reading frame 45.
10	12500	Hs.148656	Glucuronidase, beta/ immunoglobulin lambda-like polypeptide 1 pseudogene.
9	11250	Hs.547271	Hypothetical protein LOC727820.
9	11250	Hs.546288	Ribosomal protein S9.
9	11250	Hs.44017	Mitochondrial ribosomal protein L41.
9	11250	Hs.410970	Myosin, light chain 5, regulatory.
8	10000	Hs.484188	ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1.
7	8750	Hs.699841	Transcribed locus, moderately similar to XP_001116723.1 PREDICTED: immunoglobulin heavy constant alpha 2 (A2m marker) [Macaca mulatta].
7	8750	Hs.656457	CDNA FLJ37852 fis, clone BRSSN2014513.
7	8750	Hs.6396	Jumping translocation breakpoint.
7	8750	Hs.158688	Eukaryotic translation initiation factor 5B.
7	8750	Hs.107911	ATP-binding cassette, sub-family B (MDR/TAP), member 6.
6	7500	Hs.666899	Transcribed locus.
6	7500	Hs.521056	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2.
6	7500	Hs.418175	Vacuolar protein sorting 28 homolog (S. cerevisiae).
6	7500	Hs.380953	Ribosomal protein L38.
6	7500	Hs.375217	Ring finger protein 31.
5	6250	Hs.712977	Transcribed locus.
5	6250	Hs.654427	Dihydroorotate dehydrogenase.
5	6250	Hs.654258	Transcribed locus.
5	6250	Hs.645535	Gamma-glutamyltransferase 1.
5	6250	Hs.516157	Methionine adenosyltransferase II, alpha.
5	6250	Hs.454906	Gamma-glutamyltransferase light chain 3.
5	6250	Hs.430502	Transcribed locus, moderately similar to XP_544349.2 PREDICTED: similar to cAMP-specific 3,5-cyclic phosphodiesterase 4D (DPDE3) (PDE43) [Canis familiaris].
5	6250	Hs.333388	Eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein).
4	5000	Hs.709576	Hypothetical protein LOC100131998.
4	5000	Hs.668387	Transcribed locus.
4	5000	Hs.654864	HOP homeobox.
4	5000	Hs.654816	Jumonji domain containing 2B.
4	5000	Hs.654602	Syntaxin 5.
4	5000	Hs.632041	Exosome component 4.
4	5000	Hs.584921	Ceroid-lipofuscinosis, neuronal 6, late infantile, variant.
4	5000	Hs.536663	Integrin, beta 5.
4	5000	Hs.509140	Bromodomain adjacent to zinc finger domain, 1A.
4	5000	Hs.504062	Pleckstrin homology-like domain, family B, member 1.
4	5000	Hs.437178	Acyl-Coenzyme A dehydrogenase, very long chain.
4	5000	Hs.356545	Chromosome 17 open reading frame 89.
4	5000	Hs.193832	G patch domain containing 4.
4	5000	Hs.163113	Chromosome 17 open reading frame 62.
3	3750	Hs.713960	Transcribed locus, strongly similar to NP_000137.2 ferritin, light polypeptide [Homo sapiens].
3	3750	Hs.604622	Transcribed locus.
3	3750	Hs.562227	Heparan sulfate proteoglycan 2.
3	3750	Hs.499484	Similar to ribosomal protein S19.
3	3750	Hs.410378	FK506 binding protein 7.
3	3750	Hs.387208	Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed.
3	3750	Hs.3530	FUS interacting protein (serine/arginine-rich) 1.
3	3750	Hs.250615	Cytochrome P450, family 2, subfamily A, polypeptide 6.
3	3750	Hs.217079	Transcribed locus.
3	3750	Hs.156608	Transcribed locus.
3	3750	Hs.118183	Transcribed locus, strongly similar to NP_001026886.1 oligonucleotide/oligosaccharide-binding fold containing 2A [Homo sapiens].
3	3750	Hs.104476	Transmembrane protein 125.
2	2500	Hs.76716	Inter-alpha (globulin) inhibitor H3.
2	2500	Hs.75528	Guanine nucleotide binding protein-like 2 (nucleolar).
2	2500	Hs.712617	Polymerase (RNA) III (DNA directed) polypeptide H (22.9kD).
2	2500	Hs.709835	Transcribed locus.
2	2500	Hs.709182	Cell division cycle 2-like 2 (PITSLRE proteins).
2	2500	Hs.687825	Transcribed locus.
2	2500	Hs.687823	Transcribed locus.
2	2500	Hs.680921	Transcribed locus.
2	2500	Hs.668523	Transcribed locus.
2	2500	Hs.668416	Transcribed locus.
2	2500	Hs.666907	Transcribed locus.
2	2500	Hs.666892	Transcribed locus.
2	2500	Hs.604208	Transcribed locus.
2	2500	Hs.595072	Translocase of outer mitochondrial membrane 22 homolog (yeast).
2	2500	Hs.561402	Transcribed locus.
2	2500	Hs.549850	Transcribed locus.
2	2500	Hs.534307	Cyclin D3.
2	2500	Hs.528713	Kinesin family member C2.
2	2500	Hs.525557	Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1.
2	2500	Hs.524690	Peptidylprolyl isomerase E (cyclophilin E).
2	2500	Hs.522090	Galactose-1-phosphate uridylyltransferase.
2	2500	Hs.519672	Treacher Collins-Franceschetti syndrome 1.
2	2500	Hs.516948	Papillary renal cell carcinoma (translocation-associated).
2	2500	Hs.511952	Transcribed locus.
2	2500	Hs.460298	Polymerase (RNA) III (DNA directed) polypeptide E (80kD).
2	2500	Hs.406620	Ribosomal protein S10.
2	2500	Hs.374257	ST3 beta-galactoside alpha-2,3-sialyltransferase 1.
2	2500	Hs.371720	Spleen tyrosine kinase.
2	2500	Hs.371617	Polycomb group ring finger 2.
2	2500	Hs.353175	1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta).
2	2500	Hs.336994	Metastasis suppressor 1.
2	2500	Hs.269654	Transcribed locus.
2	2500	Hs.21590	Chromosome 7 open reading frame 64.